以新鲜紫皮洋葱为原料,采用热水浸提法提取洋葱低聚糖并对提取物进行分离研究。通过单因素实验及正交试验确定洋葱低聚糖的最佳提取工艺,利用薄层层析法(TLC检测)和质谱(MS)分析检测提取物,Bio gel P-2生物胶层析对糖分进行分离。结果表...以新鲜紫皮洋葱为原料,采用热水浸提法提取洋葱低聚糖并对提取物进行分离研究。通过单因素实验及正交试验确定洋葱低聚糖的最佳提取工艺,利用薄层层析法(TLC检测)和质谱(MS)分析检测提取物,Bio gel P-2生物胶层析对糖分进行分离。结果表明:紫皮洋葱低聚糖的最佳提取工艺为料液比1∶5(g/m L)、提取温度70℃、提取时间80 min,在此提取工艺下,所提取到的低聚糖含量为3.92 mg/g。TLC检测分析和MS检测表明,紫皮洋葱提取物中有多种聚合度的糖类物质存在,HPLC分析显示,紫皮洋葱低聚糖主要组成为蔗果低聚糖,β-呋喃果糖苷酶能较好的对其水解,且凝胶层析结果表明Bio gel P-2凝胶可以对紫皮洋葱低聚糖进行初步分离。展开更多
For the stability requirement of numerical resultants, the mathematical theory of classical mixed methods are relatively complex. However, generalized mixed methods are automatically stable, and their building process...For the stability requirement of numerical resultants, the mathematical theory of classical mixed methods are relatively complex. However, generalized mixed methods are automatically stable, and their building process is simple and straightforward. In this paper, based on the seminal idea of the generalized mixed methods, a simple, stable, and highly accurate 8-node noncompatible symplectic element(NCSE8) was developed by the combination of the modified Hellinger-Reissner mixed variational principle and the minimum energy principle. To ensure the accuracy of in-plane stress results, a simultaneous equation approach was also suggested. Numerical experimentation shows that the accuracy of stress results of NCSE8 are nearly the same as that of displacement methods, and they are in good agreement with the exact solutions when the mesh is relatively fine. NCSE8 has advantages of the clearing concept, easy calculation by a finite element computer program, higher accuracy and wide applicability for various linear elasticity compressible and nearly incompressible material problems. It is possible that NCSE8 becomes even more advantageous for the fracture problems due to its better accuracy of stresses.展开更多
文摘以新鲜紫皮洋葱为原料,采用热水浸提法提取洋葱低聚糖并对提取物进行分离研究。通过单因素实验及正交试验确定洋葱低聚糖的最佳提取工艺,利用薄层层析法(TLC检测)和质谱(MS)分析检测提取物,Bio gel P-2生物胶层析对糖分进行分离。结果表明:紫皮洋葱低聚糖的最佳提取工艺为料液比1∶5(g/m L)、提取温度70℃、提取时间80 min,在此提取工艺下,所提取到的低聚糖含量为3.92 mg/g。TLC检测分析和MS检测表明,紫皮洋葱提取物中有多种聚合度的糖类物质存在,HPLC分析显示,紫皮洋葱低聚糖主要组成为蔗果低聚糖,β-呋喃果糖苷酶能较好的对其水解,且凝胶层析结果表明Bio gel P-2凝胶可以对紫皮洋葱低聚糖进行初步分离。
基金the National Key Scientific Research Project,China(Nos.2018YFC1901601,2018YFC1901602)the Fundamental Research Funds for the Central Universities of Central South University,China(No.2021zzts0307)+2 种基金the National Natural Science Foundation of China(No.51804340)the Innovation-driven Plan of Central South University,China(No.2018CX036)the Collaborative Innovation Center for Clean and Efficient Utilization of Strategic Metal Mineral Resources,China,and Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources,China(No.2018TP1002).
基金supported by the National Natural Science Foundations of China (Grant 11502286)
文摘For the stability requirement of numerical resultants, the mathematical theory of classical mixed methods are relatively complex. However, generalized mixed methods are automatically stable, and their building process is simple and straightforward. In this paper, based on the seminal idea of the generalized mixed methods, a simple, stable, and highly accurate 8-node noncompatible symplectic element(NCSE8) was developed by the combination of the modified Hellinger-Reissner mixed variational principle and the minimum energy principle. To ensure the accuracy of in-plane stress results, a simultaneous equation approach was also suggested. Numerical experimentation shows that the accuracy of stress results of NCSE8 are nearly the same as that of displacement methods, and they are in good agreement with the exact solutions when the mesh is relatively fine. NCSE8 has advantages of the clearing concept, easy calculation by a finite element computer program, higher accuracy and wide applicability for various linear elasticity compressible and nearly incompressible material problems. It is possible that NCSE8 becomes even more advantageous for the fracture problems due to its better accuracy of stresses.
