目的基于人群资料构建肾肉瘤样癌预后预测模型,进行个体化预后评估。方法筛选2004至2016年美国Surveillance、Epidemiology及End Results数据库中的984例肾肉瘤样癌患者作为研究对象,采用单因素和多因素Fine and Gray和Cox比例风险回归...目的基于人群资料构建肾肉瘤样癌预后预测模型,进行个体化预后评估。方法筛选2004至2016年美国Surveillance、Epidemiology及End Results数据库中的984例肾肉瘤样癌患者作为研究对象,采用单因素和多因素Fine and Gray和Cox比例风险回归模型确定肾肉瘤样癌的独立预后风险因素,并用Nomogram构建预测模型,同时运用Calibration检测预测一致性。结果所有患者进行了根治性肾切除术,中位总生存期为11个月,多因素竞争风险模型回归分析提示肿瘤直径(HR=1.03,95%CI:1.01~1.06,P<0.001)、肿瘤T分期(T3相对T1;HR=1.56,95%CI:1.17~2.08,P<0.001)、N分期(HR=1.70,95%CI:1.43~2.04,P<0.001)、M分期(HR=2.09,95%CI:1.78~2.46,P<0.001)是肉瘤样癌预后的独立预测因素。根据其结果建立Nomogram模型,该模型预测能力较好(C-index=0.763)。结论成功构建肉瘤样癌肿瘤特异性死亡风险预后预测模型,为该类疾病提供个体化预后预测信息,有一定临床实际应用价值;同时该研究存在一定的局限性,需要外部数据进行验证。展开更多
Background Neuroblastoma （NB） is one of the most common malignant solid tumors of childhood.It is still not clear whether the apoptosis of tumor cells or the non-tumor cells contributes to the increase of concentrat...Background Neuroblastoma （NB） is one of the most common malignant solid tumors of childhood.It is still not clear whether the apoptosis of tumor cells or the non-tumor cells contributes to the increase of concentration of cytochrome c （Cyt c） in the serum of the cancer patients.The aim of this research was to identify the source of the Cyt c in the serum when the tumor grows up by subcutaneous inoculation of human NB cells into nude mice.Methods We subcutaneously inoculated human NB cells （KP-N-NS） into nude mice and collected the sera of tumor-bearing mice （n=14） and control mice （n=25） 4 weeks later in order to screen for and identify differentially expressed proteins in the serum.Differentially expressed proteins in the serum were screened by surface-enhanced laser desorption/ionization-time-of-fiight （SELDI-TOF） mass spectrometry.Results The relative intensity of a protein having a mass-to-charge ratio （m/z） of 11 609 was 3338.37±3410.85 in the tumor group and 59.84±40.74 in the control group,indicating that the expression level of this protein in the tumor group was 55.8 times higher than that in the control group.Serum proteins were separated and purified by high-performance liquid chromatography （HPLC）.Liquid chromatography coupled with tandem mass spectrometry （LC-MS/MS） was performed to produce peptide mass fingerprints （PMFs）.Spectrum analysis and a database search revealed that the highly expressed protein （m/z=11605.4） from the serum of tumor-bearing mice was the mouse Cyt c.Conclusions Increased concentration of Cyt c in the serum of tumor-bearing nude mice might be partially attributed to the secretion of this protein by non-tumor cells.展开更多
Background Wilms＇ tumor （nephroblastoma） is a cancer of the kidneys that occurs typically in children and rarely in adults. Early diagnosis is very important for the treatment and prognosis of the disease. The aim ...Background Wilms＇ tumor （nephroblastoma） is a cancer of the kidneys that occurs typically in children and rarely in adults. Early diagnosis is very important for the treatment and prognosis of the disease. The aim of our study was to discover and identify potential non-invasive and convenient biomarkers for the diagnosis of Wilms＇ tumor. Methods Nude mice were used to construct a Wilms＇ tumor model by injecting nephroblastoma cells into their bilateral abdomen. We collected 94 serum samples from mice consisting of 45 samples with Wilms＇ tumor and 49 controls. The serum proteomic profiles of the samples were analyzed via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. The candidate biomarkers were purified by high-performance liquid chromatography, identified by liquid chromatography-mass spectrometry, and validated using ProteinChip immunoassays. Results We finally retrieved two differential proteins （mlz 4509.2; 6207.9）, which were identified as apolipoprotein A-II and polyubiquitin, respectively. The expression of apolipoprotein A-II was higher in the Wilms＇ tumor group than in the control group （P 〈0.01）. By contrast, the expression of polyubiquitin was lower in the Wilms＇ tumor group than in the control group. Conclusion Apolipoprotein A-II and polyubiquitin may be used as potential biomarkers for nephroblastoma in children, and the analysis of apolipoprotein A-II may help diagnose and treat Wilms＇ tumor.展开更多
文摘目的基于人群资料构建肾肉瘤样癌预后预测模型,进行个体化预后评估。方法筛选2004至2016年美国Surveillance、Epidemiology及End Results数据库中的984例肾肉瘤样癌患者作为研究对象,采用单因素和多因素Fine and Gray和Cox比例风险回归模型确定肾肉瘤样癌的独立预后风险因素,并用Nomogram构建预测模型,同时运用Calibration检测预测一致性。结果所有患者进行了根治性肾切除术,中位总生存期为11个月,多因素竞争风险模型回归分析提示肿瘤直径(HR=1.03,95%CI:1.01~1.06,P<0.001)、肿瘤T分期(T3相对T1;HR=1.56,95%CI:1.17~2.08,P<0.001)、N分期(HR=1.70,95%CI:1.43~2.04,P<0.001)、M分期(HR=2.09,95%CI:1.78~2.46,P<0.001)是肉瘤样癌预后的独立预测因素。根据其结果建立Nomogram模型,该模型预测能力较好(C-index=0.763)。结论成功构建肉瘤样癌肿瘤特异性死亡风险预后预测模型,为该类疾病提供个体化预后预测信息,有一定临床实际应用价值;同时该研究存在一定的局限性,需要外部数据进行验证。
文摘Background Neuroblastoma （NB） is one of the most common malignant solid tumors of childhood.It is still not clear whether the apoptosis of tumor cells or the non-tumor cells contributes to the increase of concentration of cytochrome c （Cyt c） in the serum of the cancer patients.The aim of this research was to identify the source of the Cyt c in the serum when the tumor grows up by subcutaneous inoculation of human NB cells into nude mice.Methods We subcutaneously inoculated human NB cells （KP-N-NS） into nude mice and collected the sera of tumor-bearing mice （n=14） and control mice （n=25） 4 weeks later in order to screen for and identify differentially expressed proteins in the serum.Differentially expressed proteins in the serum were screened by surface-enhanced laser desorption/ionization-time-of-fiight （SELDI-TOF） mass spectrometry.Results The relative intensity of a protein having a mass-to-charge ratio （m/z） of 11 609 was 3338.37±3410.85 in the tumor group and 59.84±40.74 in the control group,indicating that the expression level of this protein in the tumor group was 55.8 times higher than that in the control group.Serum proteins were separated and purified by high-performance liquid chromatography （HPLC）.Liquid chromatography coupled with tandem mass spectrometry （LC-MS/MS） was performed to produce peptide mass fingerprints （PMFs）.Spectrum analysis and a database search revealed that the highly expressed protein （m/z=11605.4） from the serum of tumor-bearing mice was the mouse Cyt c.Conclusions Increased concentration of Cyt c in the serum of tumor-bearing nude mice might be partially attributed to the secretion of this protein by non-tumor cells.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 81071782） and the Institution of Higher Education Specialized Research Fund for Doctor （No. 200804590004）.
文摘Background Wilms＇ tumor （nephroblastoma） is a cancer of the kidneys that occurs typically in children and rarely in adults. Early diagnosis is very important for the treatment and prognosis of the disease. The aim of our study was to discover and identify potential non-invasive and convenient biomarkers for the diagnosis of Wilms＇ tumor. Methods Nude mice were used to construct a Wilms＇ tumor model by injecting nephroblastoma cells into their bilateral abdomen. We collected 94 serum samples from mice consisting of 45 samples with Wilms＇ tumor and 49 controls. The serum proteomic profiles of the samples were analyzed via surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. The candidate biomarkers were purified by high-performance liquid chromatography, identified by liquid chromatography-mass spectrometry, and validated using ProteinChip immunoassays. Results We finally retrieved two differential proteins （mlz 4509.2; 6207.9）, which were identified as apolipoprotein A-II and polyubiquitin, respectively. The expression of apolipoprotein A-II was higher in the Wilms＇ tumor group than in the control group （P 〈0.01）. By contrast, the expression of polyubiquitin was lower in the Wilms＇ tumor group than in the control group. Conclusion Apolipoprotein A-II and polyubiquitin may be used as potential biomarkers for nephroblastoma in children, and the analysis of apolipoprotein A-II may help diagnose and treat Wilms＇ tumor.
