Chinese herbal medicines(CHMs)are playing important roles in the treatment of diseases and human health care throughout the world.However,the extrinsic harmful residues have been threatening the therapies by destroy...Chinese herbal medicines(CHMs)are playing important roles in the treatment of diseases and human health care throughout the world.However,the extrinsic harmful residues have been threatening the therapies by destroying CHMs qualtiy which hinders the international trades of CHMs.This review summarized the extrinsic harmful residues contaminating CHMs in types,detection methods,national and international regulations,and made safety evaluations according to their contaminated levels,aiming to establish understanding among nations or organizations and provide references for the formulation or amendment of relative regulations.展开更多
Objectives:An enzyme-linked immunosorbent assay(ELISA)and colloidal gold-based immunochromatographic(ICG)strip assay will be developed for the rapid and high-throughput detection of atrazine(ATZ)in medicinal herbs.Met...Objectives:An enzyme-linked immunosorbent assay(ELISA)and colloidal gold-based immunochromatographic(ICG)strip assay will be developed for the rapid and high-throughput detection of atrazine(ATZ)in medicinal herbs.Methods:A monoclonal antibody against ATZ was obtained after the immunization of mice,cell fusion,and hybridoma screening,and the antibody was used to develop direct competitive ELISA(dcELISA)and the ICG strip assay.Results:Both dcELISA and ICG strip methods were established,optimized,and validated for the detection of ATZ in Salviae miltiorrhizae radix et rhizome,Astragali radix,and Isatidis radix.dcELISA had a half-maximum inhibition concentration of 10.56 ng/m L(Salviae miltiorrhizae radix et rhizome),7.6 ng/m L(Astragali radix),and 8.15 ng/m L(Isatidis radix).The limit of detection(LOD)of the ICG strip assay was 12.5 ng/mL(Salviae miltiorrhizae radix et rhizome),12.5 ng/mL(Astragali radix),and 6.25 ng/mL(Isatidis radix)in different herb matrices.Due to the recognition characteristics of the monoclonal antibody for the pesticides ATZ,propazine,sebuthylazine,and prometryn,the detection results of real samples by the two immunoassays were confirmed by liquid chromatography–tandem mass spectrometry,which proved the accuracy and reliability of the established methods.Conclusion:The proposed dcELISA and ICG strip methods were suitable for the rapid,convenient,and high-throughput detection of ATZ in these medicinal herbs.展开更多
基金supported by CAMS Innovation Fund for Medical Sciences (No. 2016-I2M-1-012, 2017-I2M-1-013)National Natural Science Foundation of China (No. 81573595, 81703699)National Project for Standardization of Chinese Materia Medica (No. ZYBZH-Y-JIN-34)
文摘Chinese herbal medicines(CHMs)are playing important roles in the treatment of diseases and human health care throughout the world.However,the extrinsic harmful residues have been threatening the therapies by destroying CHMs qualtiy which hinders the international trades of CHMs.This review summarized the extrinsic harmful residues contaminating CHMs in types,detection methods,national and international regulations,and made safety evaluations according to their contaminated levels,aiming to establish understanding among nations or organizations and provide references for the formulation or amendment of relative regulations.
基金supported by the National Natural Science Foundation of China(No.81573595)CAMS Innovation Fund for Medical Sciences(2017-I2M-1-013)High-End Foreign Experts Project(No.G20190001644)。
文摘Objectives:An enzyme-linked immunosorbent assay(ELISA)and colloidal gold-based immunochromatographic(ICG)strip assay will be developed for the rapid and high-throughput detection of atrazine(ATZ)in medicinal herbs.Methods:A monoclonal antibody against ATZ was obtained after the immunization of mice,cell fusion,and hybridoma screening,and the antibody was used to develop direct competitive ELISA(dcELISA)and the ICG strip assay.Results:Both dcELISA and ICG strip methods were established,optimized,and validated for the detection of ATZ in Salviae miltiorrhizae radix et rhizome,Astragali radix,and Isatidis radix.dcELISA had a half-maximum inhibition concentration of 10.56 ng/m L(Salviae miltiorrhizae radix et rhizome),7.6 ng/m L(Astragali radix),and 8.15 ng/m L(Isatidis radix).The limit of detection(LOD)of the ICG strip assay was 12.5 ng/mL(Salviae miltiorrhizae radix et rhizome),12.5 ng/mL(Astragali radix),and 6.25 ng/mL(Isatidis radix)in different herb matrices.Due to the recognition characteristics of the monoclonal antibody for the pesticides ATZ,propazine,sebuthylazine,and prometryn,the detection results of real samples by the two immunoassays were confirmed by liquid chromatography–tandem mass spectrometry,which proved the accuracy and reliability of the established methods.Conclusion:The proposed dcELISA and ICG strip methods were suitable for the rapid,convenient,and high-throughput detection of ATZ in these medicinal herbs.