Evolutionary timescale of chalcidoid wasps inferred from over one hundred mitochondrial genomes

Chalcidoidea is one of the most biologically diverse groups among Hymenoptera.Members are characterized by extraordinary parasitic lifestyles and extensive host ranges,among which several species attack plants or serve as pollinators.However,higher-level chalcidoid relationships remain controversial.Here,we performed mitochondrial phylogenomic analyses for major clades(18out of 25 families)of Chalcidoidea based on 139 mitochondrial genomes.The compositional heterogeneity and conflicting backbone relationships in Chalcidoidea were assessed using various datasets and tree inferences.Our phylogenetic results supported the monophyly of 16families and polyphyly of Aphelinidae and Pteromalidae.Our preferred topology recovered the relationship(Mymaridae+(Signiphoridae+Leucospidae)+(Chalcididae+((Perilampidae+Eucharitidae)+remaining Chalcidoidea))).The monophyly of Agaonidae and Sycophaginae was rejected,while the gall-associated((Megastigmidae+Ormyridae)+(Ormocerinae+Eurytomidae))relationship was supported in most results.A six-gene inversion may be a synapomorphy for most families,whereas other derived gene orders may introduce confusion in phylogenetic signals at deeper nodes.Dating estimates suggested that Chalcidoidea arose near the Jurassic/Cretaceous boundary and that two dynamic shifts in diversification occurred during the evolution of Chalcidoidea.We hypothesized that the potential codiversification between chalcidoids and their hosts may be crucial for accelerating the diversification of Chalcidoidea.Ancestral state reconstruction analyses supported the hypothesis that gallinducers were mainly derived from parasitoids of gallinducers,while other gall-inducers were derived from phytophagous groups.Taken together,these findings advance our understanding of mitochondrial genome evolution in the major interfamilial phylogeny of Chalcidoidea.

Effect of 0.5 mass% Cu Addition on Ductility and Magnetic Properties of Fe-6.5Si Alloy

The effect of adding 0.5mass% Cu on ductility and magnetic properties of Fe-6.5Si（mass%）alloy was investigated.The alloys with and without 0.5mass% Cu addition were warm rolled into thin sheets of thickness no more than 0.3mm at temperature below 600 ℃.It was found that the alloy with 0.5mass% Cu addition was more easily warm rolled than Cu-free alloy.Tensile tests were carried out to further investigate this phenomenon,which confirmed that the ductility of the alloy with 0.5mass% Cu addition was significantly higher than that of Cu-free alloy at 550 ℃.Based on the results of transmission electron microscopy analysis,the ductility increase of the alloy with 0.5mass% Cu addition was attributed to the effect of Cu on the promotion of dynamic recovery and suppression of long-range order in the alloy during warm rolling process.It was also observed that the iron loss was lower and inductance was higher for the alloy with 0.5 mass% Cu addition.Thus,it can be concluded that adding a suitably small amount of Cu would not only increase the ductility of Fe-6.5Si alloy at warm rolling temperatures but also improve its magnetic properties.