Plants can synthesize a wide range of terpenoids in response to various environmental cues.However,the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive.In th...Plants can synthesize a wide range of terpenoids in response to various environmental cues.However,the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive.In this study,we employed single-cell RNA sequencing to comprehensively characterize the transcriptome profile of cotton leaves and established a hierarchical transcriptional network regulating cellspecific terpenoid production.We observed substantial expression levels of genes associated with the biosynthesis of both volatile terpenes(such asβ-caryophyllene andβ-myrcene)and non-volatile gossypol-type terpenoids in secretory glandular cells.Moreover,two novel transcription factors,namely GoHSFA4a and GoNAC42,are identified to function downstream of the Gossypium PIGMENT GLAND FORMATION genes.Both transcription factors could directly regulate the expression of terpenoid biosynthetic genes in secretory glandular cells in response to developmental and environmental stimuli.For convenient retrieval of the single-cell RNA sequencing data generated in this study,we developed a user-friendly web server.Our findings not only offer valuable insights into the precise regulation of terpenoid biosynthesis genes in cotton leaves but also provide potential targets for cotton breeding endeavors.展开更多
The reactive electrophilic species(RES), typically the molecules bearing α,β-unsaturated carbonyl group, are widespread in living organisms and notoriously known for their damaging effects. Many of the mycotoxins re...The reactive electrophilic species(RES), typically the molecules bearing α,β-unsaturated carbonyl group, are widespread in living organisms and notoriously known for their damaging effects. Many of the mycotoxins released from phytopathogenic fungi are RES and their contamination to cereals threatens food safety worldwide. However, due to their high reactivity, RES are also used by host organisms to synthesize specific metabolites. The evolutionary conserved glyoxalase(GLX) system scavenges the cytotoxic α-oxoaldehydes that bear RES groups, which cause host disorders and diseases. In cotton, a specialized enzyme derived from glyoxalase I(GLXI) through gene duplications and named as specialized GLXI(SPG), acts as a distinct type of aromatase in the gossypol pathway to transform the RES intermediates into the phenolic products. In this review, we briefly introduce the research progress in understanding the RES, especially the RES-type mycotoxins, the GLX system and SPG, and discuss their application potential in detoxification and synthetic biology.展开更多
基金the National Key R&D Program of China(2022YFF1001400)to J.-Q.H.the National Natural Science Foundation of China(32388201)to X.-Y.C.+5 种基金the Chinese Academy of Sciences(XDB27020207)to X.-Y.C.the Foundation of Youth Innovation Promotion Association of the Chinese Academy of Sciences to J.-Q.H.the Young Elite Scientists Sponsorship Program by CAST(2019QNRC001)to J.-Q.H.the Yunnan Revitalization Talent Support Program"Top Team"Project(202305AT350001)to X.-Y.C.Winall Hi-tech Seed Co.,Ltd.(GMLM2023)to X.-Y.C.the National Natural Science Foundation of China(32172041)to W.G.
文摘Plants can synthesize a wide range of terpenoids in response to various environmental cues.However,the specific regulatory mechanisms governing terpenoid biosynthesis at the cellular level remain largely elusive.In this study,we employed single-cell RNA sequencing to comprehensively characterize the transcriptome profile of cotton leaves and established a hierarchical transcriptional network regulating cellspecific terpenoid production.We observed substantial expression levels of genes associated with the biosynthesis of both volatile terpenes(such asβ-caryophyllene andβ-myrcene)and non-volatile gossypol-type terpenoids in secretory glandular cells.Moreover,two novel transcription factors,namely GoHSFA4a and GoNAC42,are identified to function downstream of the Gossypium PIGMENT GLAND FORMATION genes.Both transcription factors could directly regulate the expression of terpenoid biosynthetic genes in secretory glandular cells in response to developmental and environmental stimuli.For convenient retrieval of the single-cell RNA sequencing data generated in this study,we developed a user-friendly web server.Our findings not only offer valuable insights into the precise regulation of terpenoid biosynthesis genes in cotton leaves but also provide potential targets for cotton breeding endeavors.
基金the financial support from the National Natural Science Foundation of China(31690092,31788103 to X.Chen and 31872666 to X.Fang)the Ministry of Agriculture of China(2016ZX08010002-005 to X.Shangguan)+1 种基金the Chinese Academy of Sciences(QYZDY-SSW-SMC026 and 153D31KYSB20160074 to X.Chen)the Young Elite Scientists Sponsorship Program by CAST(2019QNRC001)。
文摘The reactive electrophilic species(RES), typically the molecules bearing α,β-unsaturated carbonyl group, are widespread in living organisms and notoriously known for their damaging effects. Many of the mycotoxins released from phytopathogenic fungi are RES and their contamination to cereals threatens food safety worldwide. However, due to their high reactivity, RES are also used by host organisms to synthesize specific metabolites. The evolutionary conserved glyoxalase(GLX) system scavenges the cytotoxic α-oxoaldehydes that bear RES groups, which cause host disorders and diseases. In cotton, a specialized enzyme derived from glyoxalase I(GLXI) through gene duplications and named as specialized GLXI(SPG), acts as a distinct type of aromatase in the gossypol pathway to transform the RES intermediates into the phenolic products. In this review, we briefly introduce the research progress in understanding the RES, especially the RES-type mycotoxins, the GLX system and SPG, and discuss their application potential in detoxification and synthetic biology.
