Pathological scarring and scleroderma,which are the most common conditions of skin fibrosis,pathologically manifest as fibroblast proliferation and extracellular matrix(ECM)hyperplasia.Fibroblast proliferation and ECM...Pathological scarring and scleroderma,which are the most common conditions of skin fibrosis,pathologically manifest as fibroblast proliferation and extracellular matrix(ECM)hyperplasia.Fibroblast proliferation and ECM hyperplasia lead to fibrotic tissue remodeling,causing an exaggerated and prolonged wound-healing response.The pathogenesis of these diseases has not been fully clarified and is unfortunately accompanied by exceptionally high medical needs and poor treatment effects.Currently,a promising and relatively low-cost treatment has emerged-adipose-derived stem cell(ASC)therapy as a branch of stem cell therapy,including ASCs and their derivatives-purified ASC,stromal vascular fraction,ASC-conditioned medium,ASC exosomes,etc.,which are rich in sources and easy to obtain.ASCs have been widely used in therapeutic settings for patients,primarily for the defection of soft tissues,such as breast enhancement and facial contouring.In the field of skin regeneration,ASC therapy has become a hot research topic because it is beneficial for reversing skin fibrosis.The ability of ASCs to control profibrotic factors as well as anti-inflammatory and immunomodulatory actions will be discussed in this review,as well as their new applications in the treatment of skin fibrosis.Although the long-term effect of ASC therapy is still unclear,ASCs have emerged as one of the most promising systemic antifibrotic therapies under development.展开更多
Microbial constituents naturally inhabiting the gastrointestinal tract may influence the homeostasis of the gut environment.The presence or overabundance of some bacterial taxa has been reported to be associated with ...Microbial constituents naturally inhabiting the gastrointestinal tract may influence the homeostasis of the gut environment.The presence or overabundance of some bacterial taxa has been reported to be associated with complex diseases,and the metabolites of certain bacteria may contribute to diverse disorders by influencing signaling pathways.Therefore,the study of gut microbial population has emerged as a crucial field and a new potential area of clinical significance. Advances in the methods of microbiota analysis have shed light upon the details including species diversity,microfloral activities as well as the entire gut microbiota.Nevertheless,comprehensive reviews on this subject are still limited.For elucidating the appropriate selection strategy of the methods to address a particular research question,we comprehensively reviewed the continuously improving technologies,classical to newly developed,and dissected their relative advantages and drawbacks.In addition,aiming at the rapidly advancing next-generation sequencing,we enumerated the improvements in mainstream platforms and made the horizontal and vertical comparison among them.Additionally,we demonstrated the four main-omics methods,which may provide further mechanistic insights into the role of microbiota,to propel phylotyping analysis to functional analysis.展开更多
基金Supported by National Natural Science Foundation of China,No.81772103 and No.82172234Shanghai Clinical Research Center of Plastic and Reconstructive Surgery supported by Science and Technology Commission of Shanghai Municipality,China,No.22MC1940300。
文摘Pathological scarring and scleroderma,which are the most common conditions of skin fibrosis,pathologically manifest as fibroblast proliferation and extracellular matrix(ECM)hyperplasia.Fibroblast proliferation and ECM hyperplasia lead to fibrotic tissue remodeling,causing an exaggerated and prolonged wound-healing response.The pathogenesis of these diseases has not been fully clarified and is unfortunately accompanied by exceptionally high medical needs and poor treatment effects.Currently,a promising and relatively low-cost treatment has emerged-adipose-derived stem cell(ASC)therapy as a branch of stem cell therapy,including ASCs and their derivatives-purified ASC,stromal vascular fraction,ASC-conditioned medium,ASC exosomes,etc.,which are rich in sources and easy to obtain.ASCs have been widely used in therapeutic settings for patients,primarily for the defection of soft tissues,such as breast enhancement and facial contouring.In the field of skin regeneration,ASC therapy has become a hot research topic because it is beneficial for reversing skin fibrosis.The ability of ASCs to control profibrotic factors as well as anti-inflammatory and immunomodulatory actions will be discussed in this review,as well as their new applications in the treatment of skin fibrosis.Although the long-term effect of ASC therapy is still unclear,ASCs have emerged as one of the most promising systemic antifibrotic therapies under development.
文摘Microbial constituents naturally inhabiting the gastrointestinal tract may influence the homeostasis of the gut environment.The presence or overabundance of some bacterial taxa has been reported to be associated with complex diseases,and the metabolites of certain bacteria may contribute to diverse disorders by influencing signaling pathways.Therefore,the study of gut microbial population has emerged as a crucial field and a new potential area of clinical significance. Advances in the methods of microbiota analysis have shed light upon the details including species diversity,microfloral activities as well as the entire gut microbiota.Nevertheless,comprehensive reviews on this subject are still limited.For elucidating the appropriate selection strategy of the methods to address a particular research question,we comprehensively reviewed the continuously improving technologies,classical to newly developed,and dissected their relative advantages and drawbacks.In addition,aiming at the rapidly advancing next-generation sequencing,we enumerated the improvements in mainstream platforms and made the horizontal and vertical comparison among them.Additionally,we demonstrated the four main-omics methods,which may provide further mechanistic insights into the role of microbiota,to propel phylotyping analysis to functional analysis.
