腹腔镜下全结肠系膜切除对右半结肠癌根治术患者手术效果及恢复情况的影响

目的探讨腹腔镜下全结肠系膜切除(CME)对右半结肠癌患者的手术效果及恢复情况的影响。方法选取2015年1月-2017年12月右半结肠癌患者70例,依据随机数字表分为腹腔镜组和开腹组,每组35例。腹腔镜组行腹腔镜下CME治疗,开腹组行开腹CME手术治疗,比较两组患者的治疗结果及恢复情况。结果两组患者术后皮质醇(COR)、促肾上腺皮质激素(ACTH)、肾上腺素(E)均明显高于术前,腹腔镜组术后COR、ACTH、E明显低于开腹组,差异均有统计学意义(P<0.05);两组患者的手术时间、淋巴结清扫数和复发率比较,差异均无统计学意义(P>0.05),腹腔镜组术中出血量、排气时间、下床时间、住院时间和并发症率明显少于开腹组,差异均有统计学意义(P<0.05)。结论腹腔镜下CME与开腹术式的治疗效果一致,且可有效减少患者的手术创伤、减轻应激反应,有利于减少患者术后并发症、缩短术后恢复时间,对于右半结肠癌患者应优先选择腹腔镜CME术式进行治疗。

Novel assays for quality evaluation of XueBiJing:Quality variability of a Chinese herbal injection for sepsis management

XueBiJing is an intravenous five-herb injection used to treat sepsis in China.The study aimed to develop a liquid chromatography-tandem mass spectrometry(LC-MS/MS)-or liquid chromatography-ultraviolet(LC-UV)-based assay for quality evaluation of XueBiJing.Assay development involved identifying marker constituents to make the assay therapeutically relevant and building a reliable one-point calibrator for monitoring the various analytes in parallel.Nine marker constituents from the five herbs were selected based on XueBiJing's chemical composition,pharmacokinetics,and pharmacodynamics.A selectivity test(for“similarity of response”)was developed to identify and minimize interference by nontarget constituents.Then,an intercept test was developed to fulfill“linearity through zero”for each analyte(absolute ratio of intercept to C response,<2%).Using the newly developed assays,we analyzed samples from 33 batches of XueBiJing,manufactured over three years,and found small batch-to-batch variability in contents of the marker constituents(4.1%-14.8%),except for senkyunolide I(26.5%).

Diffuse uterine leiomyomatosis: A case report and review of literature

BACKGROUND Diffuse uterine leiomyomatosis(DUL)is a benign uterine smooth muscle neoplasm with unknown etiology.Since DUL is rarely reported,knowledge regarding it is limited.The rate of early diagnosis is low,and DUL is often misdiagnosed as common multiple uterine leiomyomas before surgery.CASE SUMMARY A 27-year-old patient with no sexual activity presented to the Emergency Department of our hospital complaining of heavy vaginal bleeding.She had a history of uterine fibroids and menorrhagia.Pelvic examination showed a regularly enlarged uterus,similar in size to that associated with a 4-mo pregnancy.Pelvic magnetic resonance imaging(MRI)revealed numerous multiple uterine fibroids,and a transabdominal myomectomy(TM)was performed.Intraoperative exploration revealed that the myometrium was full of myoma nodules of variable sizes.Over 50 leiomyomas were removed.The pathology report confirmed leiomyoma.The patient was discharged and received a gonadotropin-releasing hormone analog(3.75 mg)for 6 mo.Ten months after surgery,the patient presented to the hospital again for abnormal uterine bleeding.MRI showed an irregular mass with a diameter of 5.2 cm without sharp demarcation in the uterine cavity.Submucosal leiomyoma was considered first,and the patient underwent a hysteroscopic myomectomy plus hymen repair.Intraoperative exploration showed that there were several leiomyomatosis masses in the cavity.Postoperative pathological exam-ination confirmed submucosal leiomyoma and necrotic and generative tissue.Although the menstrual cycle was still irregular,the patient did not have symptoms of menorrhagia for a period of 28 mo after the second surgery.CONCLUSION Individuals with DUL are easily misdiagnosed due to the lack of specific manifestations of this disease.MRI is helpful for early identification and preoperative evaluation.There is currently no unified method of diagnosis.For women who want to preserve fertility,conservative surgery should be made an option.When TM is chosen,a modified new myomectomy should be considered to avoid the drawbacks of traditional TM.

Alcohol promotes renal fibrosis by activating Nox-mediated DNA methylation of Smad7

OBJECTIVE Alcohol is mainly metabolized through liver and excreted by kidney in the body.Kidney damage has been considered as the secondary to liver injury and kidney dysfunction is common in hospitalized patients with severe alcoholic hepatitis.Both acute and chronic alcoholism accumulation can compromise kidney function,although alcoholic kidney disease has drawn much more attention recently,the methodology for establishing the in vivo and in vitro alcoholic renal fibrosis models are still lacking,and the underlying mechanisms are to be determined.METHODS and RESULTS Mice were feed with a liquid diet containing alcohol for 4 weeks,8 weeks and 12 weeks respectively,results of Masson′ s Trichrome staining showed that kidney fibrosis peaked in 8-week model group,which consistent with the results of albumin assay,Western blot,immunostaining and real-time PCR of collagen I and α-SMA.In vitro study also confirmed that ethanol upregulated the level of fibrotic index.es,including collagen I and α-SMA,in tubular epithelial cells(HK2 cells).Additionally,both in vivo and in vitro studies showed that Smad7 was decreased and Smad3 was highly activated.Then we further detected the underlying mechanisms by which alcohol induced the imbalance of Smad7 and Smad3.Results of Genome-wide methylation sequencing found DNA methylation of Smad7 in the alcoholic fibrosis kidney,which may be mainly mediated by DNA methyltransferase 1(DNMT1),because knock.down of DNMT1,but not DNMT2 and 3,largely restored Smad7 level in ethanol-treated HK2 cells.Con.sequently,we found that NADPH Oxidases(nox)-mediated oxidative stress is the major force upregu.lating DNMT1,since knockdown of Nox2 and 4 could both decrease DNMT1 while rebalancing Smad7/Smad3 axis,and thereby relieved ethanol-induced fibrotic response in HK2 cells.More importantly,intraperitoneal injection of apocynin,an inhibitor of NADPH oxidoreductase,attenuated renal fibrosis in alcoholic kidney fibrosis mouse model.CONCLUSION By establishing the novel in vivo and in vitro models,we found that through activating oxidative stress-induced DNA methylation of Smad7,alcohol induces renal fibrosis by breaking the balance between Smad7 and Smad3.Elimination of Nox-mediated oxidative stress may be a potential therapy for treatment of long-term alcohol abuse-induced kidney fibrosis.

A novel Nance-Horan syndrome mutation identified by next-generation sequencing in a Chinese family

AIM: To identify the disease-causing mutation in a fourgeneration Chinese family diagnosed with Nance-Horan syndrome(NHS). METHODS: A Chinese family, including four affected patients and four healthy siblings, was recruited. All family members received ophthalmic examinations with medical histories provided. Targeted next-generation sequencing approach was conducted on the two affected males to screen for their disease-causing mutations. RESULTS: Two male family members diagnosed with NHS manifested bilateral congenital cataracts microcornea, strabismus and subtle facial and dental abnormalities, while female carriers presented posterior Y-sutural cataracts. A novel frameshift mutation(c.3916_3919 del) in the NHS gene was identified. This deletion was predicted to alter the reading frame and generate a premature termination codon after a new reading frame. CONCLUSION: The study discovers a new frameshift mutation in a Chinese family with NHS. The findings broaden the spectrum of NHS mutations that can cause NHS in Chinese patients.

钴-钼双金属碳化物修饰隔膜强化锂硫电池中多硫化锂的吸附和催化转化

本文报道了一种钴-钼双金属碳化物(Co3Mo3C)催化材料用于修饰锂硫电池隔膜,强化多硫化锂的化学吸附和催化转化.所组装的电池表现出优异的电化学性能,即使在8.0 mg cm^-2的硫面积负载量条件下,面积比容量仍高达6.8 mA h cm^-2.理论计算结果表明,相比于单一金属碳化物Mo2C,双金属碳化物Co3Mo3C具有更多的活性位点,更利于化学固定多硫化锂,并催化多硫化锂间相互转化;同时,Ni3Mo3C和Fe3Mo3C亦表现出类似的高催化活性.本研究对高性能锂硫电池关键催化材料的设计具有一定的指导意义.

Enhanced simultaneous absorption of NO_(x) and SO_(2) in oxidation-reduction-absorption process with a compounded system based on Na_(2)SO_(3)

Oxidation of sulfite and competitive absorption existed in Na_(2)SO_(3) solution for simultaneous removal of NO_(x) and SO_(2),inhibited the long-term high-efficiency when used for practical applications.A matching strategy was developed to solve these problems.Antioxidants combination was used to retard the oxidation of antioxidant and enhance inhibition of S(IV)(tetravalent sulfur)oxidation.Hydroquinone(HQ)and sodium thiosulfate(ST)showed a positive synergistic effect on inhibition of S(IV)oxidation.When SO_(2) concentration was 500 and 2000 ppmV,the addition of 0.1 wt.%HQ and 1 wt.%ST decreased the percentage of S(IV)oxidized by oxygen by over 30%and 40%,respectively.Alkali(Na_(2)CO_(3))alleviated the competitive absorption between NO_(x) and SO_(2).Moreover,Na_(2)CO_(3) exhibited an enhancement effect on the absorption of NO_(x) and SO_(2) when coupled with anti-oxidants.While the increase of oxygen pressure accelerated the oxidation of S(IV),the anti-oxidants can retard the oxidation.The measurement of pH suggested the removal efficiency of NO_(x) highly depended on SO_(3)^(2-) concentration rather than pH.The further investigation of the mechanism suggested the match effect was related to the interaction between ST and the intermediate product of HQ.The match strategy holds a potential for application of SO_(3)^(2-) to denitration.