Clinical significance and potential application of cuproptosis-related genes in gastric cancer

BACKGROUND Worldwide,gastric cancer(GC)is a common lethal solid malignancy with a poor prognosis.Cuproptosis is a novel type of cell death mediated by protein lipoylation and may be related to GC prognosis.AIM To offer new insights to predict GC prognosis and provide multiple therapeutic targets related to cuproptosis-related genes(CRGs)for future therapy.METHODS We collected data from several public data portals,systematically estimated the expression level and prognostic values of CRGs in GC samples,and investigated related mechanisms using public databases and bioinformatics.RESULTS Our results revealed that FDX1,LIAS,and MTF1 were differentially expressed in GC samples and exhibited important prognostic significance in The Cancer Genome Atlas(TCGA)cohort.We constructed a nomogram model for overall survival and disease-specific survival prediction and validated it via calibration plots.Mecha-nistically,immune cell infiltration and DNA methylation prominently affected the survival time of GC patients.Moreover,protein-protein interaction network,KEGG pathway and gene ontology enrichment analyses demonstrated that FDX1,LIAS,MTF1 and related proteins play key roles in the tricarboxylic acid cycle and cuproptosis.Gene Expression Omnibus database validation showed that the expression levels of FDX1,LIAS,and MTF1 were consistent with those in the TCGA cohort.Top 10 perturbagens has been filtered CONCLUSION In conclusion,FDX1,LIAS,and MTF1 could serve as potential prognostic biomarkers for GC patients and provide novel targets for immunotarget therapy.

Signet ring cell carcinoma hidden beneath large pedunculated colorectal polyp:A case report

BACKGROUND Large pedunculated colorectal polyps are not frequent among colonic polyps.We present a clinical case of a large pedunculated colorectal polyp with signet ring cell cancer infiltrating the submucosa and lymph node invasion in a patient who ultimately underwent additional surgery.Clinicians should attach importance to pedunculated colorectal polyps and choose the most appropriate therapy.CASE SUMMARY A 52-year-old female farmer underwent routine screening colonoscopy and denied constipation,diarrhea,hematochezia,or other gastrointestinal symptoms.Her past medical history and general biochemical examination results were unremarkable.During the colonoscopy,a 25-mm pedunculated polyp in the sigmoid colon was identified.The superficial epithelium was macroscopically congestive,rough,and granular,showing characteristic features of adenoma.We first ligated the root of the pedunculated polyp using nylon loops as well as a titanium clip.Histopathological examination revealed high-grade intraepithelial neoplasia of the tumor surface and a negative margin with signet ring cell adenocarcinoma infiltrating the submucosal layer.The deepest infiltration was approximately 0.9 cm from the tumor surface and 0.55 cm from the stratum basale.We performed radical resection of the left colon with lymph node dissection after two weeks.The lesion was completely resected,and pathological assessment revealed signet ring cell adenocarcinoma infiltrating the submucosal layer as well as lymph node invasion(stage PT1N1M0 and grade IIIA in pathological grading,NRAS-,BRAF V600E-,KRAS-).CONCLUSION This case highlights the importance of paying attention to the malignancy of large pedunculated polyps.Polyps or adenomas removed via endoscopy must be evaluated histologically.Even if adenomas may be fragile,endoscopy doctors should still remove polyps as completely as possible and choose perpendicular sections through the stalk and base to fix by formaldehyde solution.