To the Editor:Identifying the pathogen from clinical samples is crucial for the diagnosis of a newly emergent infectious disease,such as 2019 novel coronavirus(2019-nCoV).which has posed great threats to global public...To the Editor:Identifying the pathogen from clinical samples is crucial for the diagnosis of a newly emergent infectious disease,such as 2019 novel coronavirus(2019-nCoV).which has posed great threats to global public health.[1]In some cases,despite the positive epidemiological,clinical,and radiographic evidence,coronavirus disease 2019(COVID-19)diagnosis can still be restricted by inconclusive polymerase chain reaction(PCR)results.[2]For samples of suspicious patients,where the fluorescence quantitative PCR(FQ-PCR)results in a few respiratory tract specimens were inconclusive,high-throughput sequencing(HTS)can be an effective confirmation method.Here,we report our experience of applying HTS to confirm a suspected 2019-nCoV infection.展开更多
基金This work was supported by grants from the National Key Research and Development Program of China(Nos.2018YFC1200100 and 2018YFC1311900)science research project of the Guangdong Province(No.2019B030316028)Guangzhou Medical University High-level University Clinical Research and Cultivation Program(Nos.[2017]159 and 160).
文摘To the Editor:Identifying the pathogen from clinical samples is crucial for the diagnosis of a newly emergent infectious disease,such as 2019 novel coronavirus(2019-nCoV).which has posed great threats to global public health.[1]In some cases,despite the positive epidemiological,clinical,and radiographic evidence,coronavirus disease 2019(COVID-19)diagnosis can still be restricted by inconclusive polymerase chain reaction(PCR)results.[2]For samples of suspicious patients,where the fluorescence quantitative PCR(FQ-PCR)results in a few respiratory tract specimens were inconclusive,high-throughput sequencing(HTS)can be an effective confirmation method.Here,we report our experience of applying HTS to confirm a suspected 2019-nCoV infection.
