Indicators are the basis for judging the working performance of exhaust hood and capture performance are usually used as the only indicator.An evaluation index system including three factors of cooking oil fumes(COF)i...Indicators are the basis for judging the working performance of exhaust hood and capture performance are usually used as the only indicator.An evaluation index system including three factors of cooking oil fumes(COF)instantaneous capture,health risk impact and thermal comfort was proposed to assess the comprehensive performance of exhaust hood in the present study.The primary capture efficiency(PCE)of formaldehyde,the PCE of particulate matter with the diameter less than or equal to 2.5μm(PM_(2.5)),the incremental lifetime cancer risk(ILCR)of formaldehyde,the ILCR of PM_(2.5)and the predicted mean vote(PMV),which can all be quantified with the aid of computational fluid dynamics(CFD),were selected as the indicators.And the analytic hierarchy process(AHP)method was introduced to perform the comprehensive performance evaluation of exhaust hood.The performance of two exhaust hood structures(grille and orifice type)with three exhaust rates(3000,4000,and 5000 m^(3)/h)in two cooking zones of a university canteen kitchen were evaluated.The result showed that the reduction of ILCR of COF exposure is the most important to the performance of exhaust hood.The comprehensive performance of orifice exhaust hood with exhaust rate of 4000 and 5000 m^(3)/h are optimal;the orifice exhaust hood with exhaust of 3000 m^(3)/h and grille exhaust hood with exhaust rate of 5000 m^(3)/h are moderate;the grille exhaust hood with exhaust rate of 3000 and 4000 m^(3)/h are low.Decision-making priorities based on comprehensive and individual performance are not exactly the same in the two cooking zones.It is necessary to use the index system to evaluate the comprehensive performance of exhaust hood that considers the impact on human health and thermal comfort.展开更多
Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-savi...Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-saving.However,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension culture.In this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing processes.The hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process alone.Furthermore,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to differentiate.However,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D culture.The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing processes.Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in hASCs.We discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility.展开更多
The affiliations of several authors were modified during the proofing stage at the request of corresponding author Akon Higuchi,without notifying the involved authors and the Editorial Office.After communication with ...The affiliations of several authors were modified during the proofing stage at the request of corresponding author Akon Higuchi,without notifying the involved authors and the Editorial Office.After communication with all authors,all parties involved agreed to publish this erratum to correct the affiliations and authors.展开更多
基金supported by the National Key R&D Program of China(No.2017YFC0211502).
文摘Indicators are the basis for judging the working performance of exhaust hood and capture performance are usually used as the only indicator.An evaluation index system including three factors of cooking oil fumes(COF)instantaneous capture,health risk impact and thermal comfort was proposed to assess the comprehensive performance of exhaust hood in the present study.The primary capture efficiency(PCE)of formaldehyde,the PCE of particulate matter with the diameter less than or equal to 2.5μm(PM_(2.5)),the incremental lifetime cancer risk(ILCR)of formaldehyde,the ILCR of PM_(2.5)and the predicted mean vote(PMV),which can all be quantified with the aid of computational fluid dynamics(CFD),were selected as the indicators.And the analytic hierarchy process(AHP)method was introduced to perform the comprehensive performance evaluation of exhaust hood.The performance of two exhaust hood structures(grille and orifice type)with three exhaust rates(3000,4000,and 5000 m^(3)/h)in two cooking zones of a university canteen kitchen were evaluated.The result showed that the reduction of ILCR of COF exposure is the most important to the performance of exhaust hood.The comprehensive performance of orifice exhaust hood with exhaust rate of 4000 and 5000 m^(3)/h are optimal;the orifice exhaust hood with exhaust of 3000 m^(3)/h and grille exhaust hood with exhaust rate of 5000 m^(3)/h are moderate;the grille exhaust hood with exhaust rate of 3000 and 4000 m^(3)/h are low.Decision-making priorities based on comprehensive and individual performance are not exactly the same in the two cooking zones.It is necessary to use the index system to evaluate the comprehensive performance of exhaust hood that considers the impact on human health and thermal comfort.
基金the Researchers supporting Project number(RSP-2019/98)King Saud University,Riyadh,Saudi Arabia。
文摘Human mesenchymal stem cells,such as human adipose-derived stem cells(hASCs),are typically cultured on a two-dimensional(2 D)monolayer material surface,on which 2 D culturing methods are easily performed and time-saving.However,hASCs usually suffer from decreased pluripotency and differentiation ability when cultured with a 2 D monolayer culturing method compared to hASCs cultured with a three-dimensional(3 D)culturing method,such as suspension culture.In this study,we evaluated whether the pluripotency and differentiation ability of hASCs can be reversibly changed during sequential cultivation with 2 D and 3 D culturing processes.The hASCs cultivated with a 3 D culturing process after 2 D culture showed at least 2-fold enhanced pluripotency(Sox2,Nanog,and OCT4)compared with that of hASCs cultured with the 2 D culture process alone.Furthermore,hASCs obtained from the 3 D culture process expressed increased levels of differentiation markers of chondrocytes and osteoblasts compared with hASCs obtained from the 2 D culture process when hASCs were induced to differentiate.However,their pluripotency and differentiation ability were extensively reduced when hASCs were shifted from 3 D culture to 2 D culture and vice versa,which indicates that hASCs show reversibility in terms of their pluripotency and differentiation ability depending on their environment in 2 D and 3 D culture.The reversibility of pluripotency and differentiation ability were found to last for at least 5 passages in culture during the alternative and sequential culture of cells with 2 D and 3 D culturing processes.Our study revealed the importance of the culture microenvironment in maintaining the pluripotency and differentiation ability of hASCs,which may reduce the effects of the aging process in hASCs.We discuss whether the environment of stem cell culture(i.e.,2 D or 3 D cultivation)can affect stem cell fate in terms of pluripotency and differentiation reversibility.
文摘The affiliations of several authors were modified during the proofing stage at the request of corresponding author Akon Higuchi,without notifying the involved authors and the Editorial Office.After communication with all authors,all parties involved agreed to publish this erratum to correct the affiliations and authors.
