The fundamental transverse mode(TEM00)is preferable for experimental and theoretical study on the laser-induced retinal injury effect,for it can produce the minimal retinal image andestablish the most strict laser saf...The fundamental transverse mode(TEM00)is preferable for experimental and theoretical study on the laser-induced retinal injury effect,for it can produce the minimal retinal image andestablish the most strict laser safety standards.But actually lasers with higher order mode werefrequently used in both earlier and recent studies.Generally higher order mode leads to largerretinal spot size and so higher damage threshold,but there are few quantitative analyses on thisproblem.In this paper,a four-surface schematic eye model is established for human and macaque.The propagation of 532-nm laser in schematic eye is analyzed by the ABCD law of Gaussianoptics.It is shown that retinal spot size increases with laser transverse mode order.For relativelower mode order,the retinal spot diameter will not exceed the minimum laser-induced retinallesion(25~30μm in diameter),and so has little effect on retinal damage threshold.While forhigher order mode,the larger'retinal spot requires more energy to induce injury and so the damage threshold increases.When beam divergence is lowered,the retinal spot size decreasescorrespondingly,so the efect of mode order can be compensated.The retinal spot size of macaqueis slightly smaller than that of human and the ratio bet ween them is independent of mode order.We conclude that the laser mode order has significant influence on retinal spot size but limitedinfluence on the retinal injury ffect.展开更多
The chromosomal position effect can significantly affect the transgene expression,which may provide an efficient strategy for the inauguration of alien genes in new hosts,but has been less explored rationally.The bact...The chromosomal position effect can significantly affect the transgene expression,which may provide an efficient strategy for the inauguration of alien genes in new hosts,but has been less explored rationally.The bacterium Myxococcus xanthus harbors a large circular high-GC genome,and the position effect in this chassis may result in a thousand-fold expression variation of alien natural products.In this study,we conducted transposon insertion at TA sites on the M.xanthus genome,and used enrichment and dilution indexes to respectively appraise high and low expression potentials of alien genes at insertion sites.The enrichment sites are characteristically distributed along the genome,and the dilution sites are overlapped well with the horizontal transfer genes.We experimentally demonstrated the enrichment sites as high expression integration sites(HEISs),and the dilution sites unsuitable for gene integration expression.This work highlights that HEISs are the plug-and-play sites for efficient expression of integrated genes.展开更多
基金the support from the National Nature Science Foundation of China(NSFC),the grant number is 61275194.
文摘The fundamental transverse mode(TEM00)is preferable for experimental and theoretical study on the laser-induced retinal injury effect,for it can produce the minimal retinal image andestablish the most strict laser safety standards.But actually lasers with higher order mode werefrequently used in both earlier and recent studies.Generally higher order mode leads to largerretinal spot size and so higher damage threshold,but there are few quantitative analyses on thisproblem.In this paper,a four-surface schematic eye model is established for human and macaque.The propagation of 532-nm laser in schematic eye is analyzed by the ABCD law of Gaussianoptics.It is shown that retinal spot size increases with laser transverse mode order.For relativelower mode order,the retinal spot diameter will not exceed the minimum laser-induced retinallesion(25~30μm in diameter),and so has little effect on retinal damage threshold.While forhigher order mode,the larger'retinal spot requires more energy to induce injury and so the damage threshold increases.When beam divergence is lowered,the retinal spot size decreasescorrespondingly,so the efect of mode order can be compensated.The retinal spot size of macaqueis slightly smaller than that of human and the ratio bet ween them is independent of mode order.We conclude that the laser mode order has significant influence on retinal spot size but limitedinfluence on the retinal injury ffect.
基金the National Key Research and Development Program of China(2021YFC2101000)the National Natural Science Foundation of China(32301220).
文摘The chromosomal position effect can significantly affect the transgene expression,which may provide an efficient strategy for the inauguration of alien genes in new hosts,but has been less explored rationally.The bacterium Myxococcus xanthus harbors a large circular high-GC genome,and the position effect in this chassis may result in a thousand-fold expression variation of alien natural products.In this study,we conducted transposon insertion at TA sites on the M.xanthus genome,and used enrichment and dilution indexes to respectively appraise high and low expression potentials of alien genes at insertion sites.The enrichment sites are characteristically distributed along the genome,and the dilution sites are overlapped well with the horizontal transfer genes.We experimentally demonstrated the enrichment sites as high expression integration sites(HEISs),and the dilution sites unsuitable for gene integration expression.This work highlights that HEISs are the plug-and-play sites for efficient expression of integrated genes.
