Dear Editor, I am Dr Xiao-Yu Yang, from the Center of Clinical Reproductive Medicine in the First Affiliated Hospital at the NanJing Medical University, Nanjing, China. We present here a case report of a globo- zoosp...Dear Editor, I am Dr Xiao-Yu Yang, from the Center of Clinical Reproductive Medicine in the First Affiliated Hospital at the NanJing Medical University, Nanjing, China. We present here a case report of a globo- zoospermic patient whose partner became pregnant after intracyto- plasmic sperm injection (ICSI) with assisted oocyte activation (AOA).展开更多
The International Society of Reproductive Genetics(ISRG)assembled a workgroup made up of clinicians,clinical laboratory directors,and scientists for the purpose of creating the guidelines for preimplantation genetic t...The International Society of Reproductive Genetics(ISRG)assembled a workgroup made up of clinicians,clinical laboratory directors,and scientists for the purpose of creating the guidelines for preimplantation genetic testing(PGT).The most up-to-date information and clinical insights for the optimal PGT practice were incorporated in these guidelines.Recommendations are provided for embryologists,medical geneticists,clinical laboratorians,and other healthcare providers to improve the wellbeing of patients seeking assisted reproductive treatment and their offspring.展开更多
有序排列、取向生长的钙钛矿纳米线阵列的合成通常需要复杂的工艺条件和精密的仪器设备,这不利于其广泛应用.本文提出了一种纳米通道限域生长的方法来制备钙钛矿纳米线阵列.利用场发射扫描电子显微镜、原子力显微镜和X射线衍射等分析手...有序排列、取向生长的钙钛矿纳米线阵列的合成通常需要复杂的工艺条件和精密的仪器设备,这不利于其广泛应用.本文提出了一种纳米通道限域生长的方法来制备钙钛矿纳米线阵列.利用场发射扫描电子显微镜、原子力显微镜和X射线衍射等分析手段,表征了纳米线阵列的形貌和晶体结构,证明了该方法可用于在各种衬底上制备大面积的具有取向生长特点的不同种类的钙钛矿纳米线阵列.基于MAPb I3纳米线阵列所制备的光电探测器光响应优异,响应度和比探测率分别为20.56 A W^(-1)和4.73×10^(12)Jones.此外,由于所制备的一维钙钛矿纳米线结构的各向异性,该光电探测器还实现了对于偏振光的探测,其偏振比为2.2.展开更多
Infertility seriously endangers the reproductive health of women at childbearing age.It is defined as the failure to achieve successful pregnancy after 1 year or more of regular unprotected intercourse.Broadly defined...Infertility seriously endangers the reproductive health of women at childbearing age.It is defined as the failure to achieve successful pregnancy after 1 year or more of regular unprotected intercourse.Broadly defined,infertility includes two aspects-failure to conceive or have a live birth.This guideline only addressed content relevant to the former.It was proposed by the gynecological endocrine group of the Chinese Society of Obstetrics and Gynecology,Chinese Medical Association,based on relevant guidelines of the World Health Organization,the American Society for Reproductive Medicine,the National Institute for Health and Clinical Excellence,as well as the clinical practice in China.The guideline was reviewed by experts and doctors from medical institutions at all levels,which is applicable to the diagnosis of infertility by physicians in obstetrics,gynecology,and andrology at various medical institutions nationwide.展开更多
Objective To conduct a Meta-analysis of studies that compared the efficacies of mild ovarian stimulation and conventional long GnRH agonist protocol in patients under- going IVF or intracytoplasmic sperm injection (I...Objective To conduct a Meta-analysis of studies that compared the efficacies of mild ovarian stimulation and conventional long GnRH agonist protocol in patients under- going IVF or intracytoplasmic sperm injection (ICSI). Methods Meta-analysis was performed. All studies were published by July 2012 with data related to outcomes following mild ovarian stimulation compared with the conventional protocol. Odds ratios (ORs) and weighted/standardized mean difference (WMD/SMD) from individual study were pooled in fixed and random effect models. Main outcome measure was the efficacy of mild ovarian stimulation. Results Six articles were included in this Meta-analysis. The number of oocytes retrieved was lower, the cycle cancellation rate was higher and the incidence of ovarian hyperstimulation syndrome (OHSS) was lower in the mild stimulation group than in conventional ovarian stimulation group. Clinical pregnancy rates were similar in both mild and conventional stimulation groups. Conclusions The level of evidence supporting the use of mild stimulation protocols in IVF is low, considering the fewer oocytes retrieved and the higher rates of cycle cancellation.展开更多
Objective To investigate the neuroprotective effects and the mechanism of this protection of raloxifene (RLX), a selective estrogen receptor modulator.Methods MTT assay and flow cytometry with annexin V-FITC/PI stai...Objective To investigate the neuroprotective effects and the mechanism of this protection of raloxifene (RLX), a selective estrogen receptor modulator.Methods MTT assay and flow cytometry with annexin V-FITC/PI staining were performed to evaluate the neuroprotective effects of RLX on Aβ25-35-induced toxicity. The potential mechanisms were studied by Western blotting in cultured rat pheochromocytoma cells (PC12 cells).Results RLX(1 000 nmol/L), in combination with Aβ25-35 (30 llmol/L), increased the cell viability (P 〈0.001), and reduced the number of apoptotic cells (P 〈0.05). RLX attenuated Aβ25-35-induced loss of △ψm (P 〈0.01). The changing of △ψm was similar to the variation of apoptosis. PD98059 (inhibitor of ERK1/2) inhibited the effects of RLX on cell viability and phosphorylation of cleaved caspase-9. No significant difference of cell viability or phosphorylation of cleaved caspase-9 had been found when PC12 cells were incubated with SB203580 (inhibitor of p38MAPK) or SP600125 (inhibitor of JNK). Afl25.35 induced a time-dependent phosphorylation of p38MAPK and JNK. In PC12 cells treated solely with RLX, ERK1/2 was activated (P〈0.01). In PC12 cells treated with Aβ25-35 and RLX, Aβ2545-induced phosphorylation of p38MAPK and JNK were inhibited (P〈0.01 and P〈0.001, respectively).Conclusion RLX inhibited Af125.35-induced cell apoptosis by activating the ERK1/2 pathway in PC12 cells. RLX also attenuated Aβ25-35-induced activation of p38MAPK and JNK. The mitochondria pathway Was involved in this inhibitory effect.展开更多
Asthenozoospermia is a common cause of decreased male fertility, and several factors can inhibit or prevent sperm motility, including ultrastructural defects of the sperm flagellum) An ultrastructural defect leading ...Asthenozoospermia is a common cause of decreased male fertility, and several factors can inhibit or prevent sperm motility, including ultrastructural defects of the sperm flagellum) An ultrastructural defect leading to severe asthenozoospermia is a syndrome called dysplasia of the fibrous sheath (DFS).展开更多
Objective:The transcriptional profile of cumulus cells(CCs)during oocyte maturation provides information for predicting oocyte developmental competence.Our previous study using a mouse model indicated that there were ...Objective:The transcriptional profile of cumulus cells(CCs)during oocyte maturation provides information for predicting oocyte developmental competence.Our previous study using a mouse model indicated that there were nine different genes related to oocyte development potential expressed in CCs during oocyte maturation.The purpose of this study was to elucidate whether gene expression levels of CCs during oocyte maturation are associated with oocyte developmental competence.Methods:The human CCs collected from each oocyte were divided into two groups after tracking depending on whether or not they developed to the blastocyst stage:(1)the oocytes were developed to blastocyst stage after fertilization(B+)and(2)the oocytes were not developed to blastocyst stage after fertilization(B−).The expression levels of the nine selected genes(ARRB1,ATP2C1,CDH5,CNTNAP1,LGR4,MKLN1,RHOBTB1,SIX2,and SMC2)were examined.CCs were obtained from 29 women who were undergoing intracytoplasmic sperm injection treatment cycles.Quantitative reverse transcriptase-polymerase chain reaction analysis was performed on cumulus masses collected before insemination.Each sample was run three times.Statistically significant differences in mRNA expression of the target genes in independent samples were evaluated by two-tailed Student’s t-test,and P<0.05 was considered significantly different.Results:There were significant differences in the mRNA expression levels of ARRB1(P=0.016),LGR4(P=0.025),and SMC2(P=0.013)between the groups B+and B−.Gene expression of ARRB1,LGR4,and SMC2 in CCs is related to blastocyst development.Conclusions:Analysis of expression of ARRB1,LGR4,and SMC2 genes in CCs as biomarkers may provide predictive information on oocyte developmental competence before insemination and fertilization.展开更多
Objective To identify efficient predictors of clinical outcomes of in vitro fertilization (IVF). Methods Two hundred and one women undergoing their first IVF cycle were measured for antral follicle count (AFC) and...Objective To identify efficient predictors of clinical outcomes of in vitro fertilization (IVF). Methods Two hundred and one women undergoing their first IVF cycle were measured for antral follicle count (AFC) and endometrial thickness via ultrasound. On day 2 or 3 of the menstrual cycle and the day of human chorionic gonadotropin administration, serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, and progesterone were determined. The difference of ovarian response and clinical pregnancy rates were measured. Results Basal FSH was the single best predictor of ovarian response in women 〈35 years old before the initiation of treatment, with an area under the receiver operating characteristic curve (A UC) of 0. 752. For women ≥ 35 years old, none of parameters was a single good predictor of lVF pregnancy. For women older than 35 years, adding body mass index (BMI) with AFC and basal FSH improved prediction of ovarian response (A UC=0. 859). Prediction of clinical pregnancy for women ≥ 35 years was improved either by combining only AFC and basal FSH (AUC=0. 757) or adding BMI to the combined AFC and FSH (AUC=0. 722). Conclusion Combining AFC, basal FSH, and BMI was the best predictor of ovarian response and clinical pregnancy after IVF in women ≥ 35 years.展开更多
文摘Dear Editor, I am Dr Xiao-Yu Yang, from the Center of Clinical Reproductive Medicine in the First Affiliated Hospital at the NanJing Medical University, Nanjing, China. We present here a case report of a globo- zoospermic patient whose partner became pregnant after intracyto- plasmic sperm injection (ICSI) with assisted oocyte activation (AOA).
基金National Key Research and Development Program of China(2021YFC2700701,2021YFC2701002,2020YFA0804000,2018YFC1004901)National Natural Science Foundation of China(82171677,81901495,82088102,81971344,82171686,82071661)+6 种基金Clinical Research Project of Shanghai Municipal Health Commission(202140110)CAMS Innovation Fund for Medical Sciences(2019-I2M-5-064)International Science and Technology Collaborative Fund of Shanghai(18410711800)Collaborative Innovation Program of Shanghai Municipal Health Commission(2020CXJQ01)Shanghai Municipal Commission of Science and Technology Program(21Y21901002,22S31901500)Clinical Research Plan of SHDC(SHDC2020CR1008A)Shanghai Frontiers Science Research Base of Reproduction and Development,and Shanghai"Science and Technology Innovation Action Plan"Hong Kong,Macao,and Taiwan Science and Technology Cooperation Project(19410760100)
文摘The International Society of Reproductive Genetics(ISRG)assembled a workgroup made up of clinicians,clinical laboratory directors,and scientists for the purpose of creating the guidelines for preimplantation genetic testing(PGT).The most up-to-date information and clinical insights for the optimal PGT practice were incorporated in these guidelines.Recommendations are provided for embryologists,medical geneticists,clinical laboratorians,and other healthcare providers to improve the wellbeing of patients seeking assisted reproductive treatment and their offspring.
基金supported by the National Natural Science Foundation of China (NSFC, 62074048)the Fundamental Research Funds for the Central Universities (PA2020GDKC0014, JZ2020HGTB0051, and JZ2018HGXC0001)the Open Foundation of Anhui Provincial Key Laboratory of Advanced Functional Materials and Devices (4500-411104/011)
文摘有序排列、取向生长的钙钛矿纳米线阵列的合成通常需要复杂的工艺条件和精密的仪器设备,这不利于其广泛应用.本文提出了一种纳米通道限域生长的方法来制备钙钛矿纳米线阵列.利用场发射扫描电子显微镜、原子力显微镜和X射线衍射等分析手段,表征了纳米线阵列的形貌和晶体结构,证明了该方法可用于在各种衬底上制备大面积的具有取向生长特点的不同种类的钙钛矿纳米线阵列.基于MAPb I3纳米线阵列所制备的光电探测器光响应优异,响应度和比探测率分别为20.56 A W^(-1)和4.73×10^(12)Jones.此外,由于所制备的一维钙钛矿纳米线结构的各向异性,该光电探测器还实现了对于偏振光的探测,其偏振比为2.2.
文摘Infertility seriously endangers the reproductive health of women at childbearing age.It is defined as the failure to achieve successful pregnancy after 1 year or more of regular unprotected intercourse.Broadly defined,infertility includes two aspects-failure to conceive or have a live birth.This guideline only addressed content relevant to the former.It was proposed by the gynecological endocrine group of the Chinese Society of Obstetrics and Gynecology,Chinese Medical Association,based on relevant guidelines of the World Health Organization,the American Society for Reproductive Medicine,the National Institute for Health and Clinical Excellence,as well as the clinical practice in China.The guideline was reviewed by experts and doctors from medical institutions at all levels,which is applicable to the diagnosis of infertility by physicians in obstetrics,gynecology,and andrology at various medical institutions nationwide.
基金supported by grants from the Medical Research Council(No.XK02 200904)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Objective To conduct a Meta-analysis of studies that compared the efficacies of mild ovarian stimulation and conventional long GnRH agonist protocol in patients under- going IVF or intracytoplasmic sperm injection (ICSI). Methods Meta-analysis was performed. All studies were published by July 2012 with data related to outcomes following mild ovarian stimulation compared with the conventional protocol. Odds ratios (ORs) and weighted/standardized mean difference (WMD/SMD) from individual study were pooled in fixed and random effect models. Main outcome measure was the efficacy of mild ovarian stimulation. Results Six articles were included in this Meta-analysis. The number of oocytes retrieved was lower, the cycle cancellation rate was higher and the incidence of ovarian hyperstimulation syndrome (OHSS) was lower in the mild stimulation group than in conventional ovarian stimulation group. Clinical pregnancy rates were similar in both mild and conventional stimulation groups. Conclusions The level of evidence supporting the use of mild stimulation protocols in IVF is low, considering the fewer oocytes retrieved and the higher rates of cycle cancellation.
基金supported by the "Six Talents Peak" of Jiangsu Province,China 973 Program(No.2007CB944005)Science and Technology Development Foundation of Nanjing Medical University(No.2011NJMU152)
文摘Objective To investigate the neuroprotective effects and the mechanism of this protection of raloxifene (RLX), a selective estrogen receptor modulator.Methods MTT assay and flow cytometry with annexin V-FITC/PI staining were performed to evaluate the neuroprotective effects of RLX on Aβ25-35-induced toxicity. The potential mechanisms were studied by Western blotting in cultured rat pheochromocytoma cells (PC12 cells).Results RLX(1 000 nmol/L), in combination with Aβ25-35 (30 llmol/L), increased the cell viability (P 〈0.001), and reduced the number of apoptotic cells (P 〈0.05). RLX attenuated Aβ25-35-induced loss of △ψm (P 〈0.01). The changing of △ψm was similar to the variation of apoptosis. PD98059 (inhibitor of ERK1/2) inhibited the effects of RLX on cell viability and phosphorylation of cleaved caspase-9. No significant difference of cell viability or phosphorylation of cleaved caspase-9 had been found when PC12 cells were incubated with SB203580 (inhibitor of p38MAPK) or SP600125 (inhibitor of JNK). Afl25.35 induced a time-dependent phosphorylation of p38MAPK and JNK. In PC12 cells treated solely with RLX, ERK1/2 was activated (P〈0.01). In PC12 cells treated with Aβ25-35 and RLX, Aβ2545-induced phosphorylation of p38MAPK and JNK were inhibited (P〈0.01 and P〈0.001, respectively).Conclusion RLX inhibited Af125.35-induced cell apoptosis by activating the ERK1/2 pathway in PC12 cells. RLX also attenuated Aβ25-35-induced activation of p38MAPK and JNK. The mitochondria pathway Was involved in this inhibitory effect.
文摘Asthenozoospermia is a common cause of decreased male fertility, and several factors can inhibit or prevent sperm motility, including ultrastructural defects of the sperm flagellum) An ultrastructural defect leading to severe asthenozoospermia is a syndrome called dysplasia of the fibrous sheath (DFS).
基金Supported by The Key Project from Chinese Technology Department(Project No.2017YFC1001601).
文摘Objective:The transcriptional profile of cumulus cells(CCs)during oocyte maturation provides information for predicting oocyte developmental competence.Our previous study using a mouse model indicated that there were nine different genes related to oocyte development potential expressed in CCs during oocyte maturation.The purpose of this study was to elucidate whether gene expression levels of CCs during oocyte maturation are associated with oocyte developmental competence.Methods:The human CCs collected from each oocyte were divided into two groups after tracking depending on whether or not they developed to the blastocyst stage:(1)the oocytes were developed to blastocyst stage after fertilization(B+)and(2)the oocytes were not developed to blastocyst stage after fertilization(B−).The expression levels of the nine selected genes(ARRB1,ATP2C1,CDH5,CNTNAP1,LGR4,MKLN1,RHOBTB1,SIX2,and SMC2)were examined.CCs were obtained from 29 women who were undergoing intracytoplasmic sperm injection treatment cycles.Quantitative reverse transcriptase-polymerase chain reaction analysis was performed on cumulus masses collected before insemination.Each sample was run three times.Statistically significant differences in mRNA expression of the target genes in independent samples were evaluated by two-tailed Student’s t-test,and P<0.05 was considered significantly different.Results:There were significant differences in the mRNA expression levels of ARRB1(P=0.016),LGR4(P=0.025),and SMC2(P=0.013)between the groups B+and B−.Gene expression of ARRB1,LGR4,and SMC2 in CCs is related to blastocyst development.Conclusions:Analysis of expression of ARRB1,LGR4,and SMC2 genes in CCs as biomarkers may provide predictive information on oocyte developmental competence before insemination and fertilization.
基金supported by the open fund from State Key Laboratory of Reproductive Medicine in Nanjing Medical University(No.SKLRM-KF-1210)
文摘Objective To identify efficient predictors of clinical outcomes of in vitro fertilization (IVF). Methods Two hundred and one women undergoing their first IVF cycle were measured for antral follicle count (AFC) and endometrial thickness via ultrasound. On day 2 or 3 of the menstrual cycle and the day of human chorionic gonadotropin administration, serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, and progesterone were determined. The difference of ovarian response and clinical pregnancy rates were measured. Results Basal FSH was the single best predictor of ovarian response in women 〈35 years old before the initiation of treatment, with an area under the receiver operating characteristic curve (A UC) of 0. 752. For women ≥ 35 years old, none of parameters was a single good predictor of lVF pregnancy. For women older than 35 years, adding body mass index (BMI) with AFC and basal FSH improved prediction of ovarian response (A UC=0. 859). Prediction of clinical pregnancy for women ≥ 35 years was improved either by combining only AFC and basal FSH (AUC=0. 757) or adding BMI to the combined AFC and FSH (AUC=0. 722). Conclusion Combining AFC, basal FSH, and BMI was the best predictor of ovarian response and clinical pregnancy after IVF in women ≥ 35 years.