THE COORDINATION ADSORPTION OF AMINES ON CARBOXYL RESIN IN Cu^(2+) FORM FROM NON-AQUEOUS MEDIUM AND DESORPTION WITH ANHYDROUS ELUANT

The adsorption of aniline, N-methylaniline and N,N-dimethylaniline onto carboxyl resin in Cu2+ form from water, ethanol and n-hexane have been studied. The results show that the adsorption affinities from n-hexane are higher than that from water, and nearly zero from ethanol. The separation factors for the adsorption of these three amines from mixed solution were also examined. The results of continuous column operations show that the breakthrough capacity of aniline from n-hexane reaches 90 mg/g dry resin in Cu2+ form, and the amines adsorbed can be stripped with anhydrous ethanol effectively. Adsorption onto carboxyl resin in Cu2+ form from non-aqueous medium and desorption with anhydrous eluant can overcome the run-off of Cu2+ from the resin, and would show potential advantages in the separation of some water-insoluble natural products.

Elastic fiber degradation in the development of pediatric granuloma annulare:Report of 39 cases

BACKGROUND Granuloma annulare(GA)has diverse clinical manifestations,multiple subtypes,and unknown etiology and pathogenesis.Existing studies regarding GA in children are scarce.AIM To examine the correlation between clinical manifestation and histopathology of pediatric GA.METHODS A total of 39 patients under 18 years of age with both a clinical and pathological diagnosis of GA at Kunming Children's Hospital from 2017 to 2022 were retrieved.Their medical records were consulted,and clinical data of the children were recorded and summarized,including gender,age,disease site,etc.Existing wax blocks of skin lesion specimens of children and pathological films were retrieved for further study and relevant histology,including hematoxylin-eosin,Alcian blue,elastic fiber(Victoria blue-Lichon red method),and antacid staining.Finally,the children’s clinical manifestations,histopathological results,and special staining characteristics were analyzed.RESULTS The clinical manifestations of granuloma annulare in children were diverse:11 cases presented with a single lesion,25 with multiple lesions,and 3 with generalized lesions.The pathological typing comprised histiocytic infiltration,palisading granuloma,epithelioid nodular,and mixed types in 4,11,9,and 15 cases,respectively.Thirty-nine cases were negative for antacid staining.The positive rate of Alcian blue staining was 92.3%,and that of elastic fiber staining was 100%.The degree of elastic fiber dissolution and granuloma annulare histopathological typing were positively correlated(r=0.432,P<0.05).No correlation was found between clinical presentation and histopathological typing of the granuloma annulare in children.In the pathological diagnosis of granuloma annulare,the positive elastic fiber staining rate was higher than that of Alcian blue staining.A correlation was found between elastic fiber dissolution degree and histopathological staging.However,the differences in pathological staging may have been related to the pathological manifestation of granuloma annulare at different periods.CONCLUSION Elastic fiber degradation may be a critical step in the pathogenesis of pediatric granuloma annulare.This is also one of the first studies focused on granuloma annulare in children.