Lipid droplets(LDs),which are the hubs of lipid metabolism,play a critical role in maintaining cellular energy homeostasis.The construction of advanced photo-sensitizers(PSs)capable of manipulating LD-mediated cell fa...Lipid droplets(LDs),which are the hubs of lipid metabolism,play a critical role in maintaining cellular energy homeostasis.The construction of advanced photo-sensitizers(PSs)capable of manipulating LD-mediated cell fate regulation is highly desirable though rarely reported.In this study,a near-infrared emissive PS(DPCMP)with LDs specificity was synthesized and successfully applied to induce ferroptosis and apoptosis.DPCMP exhibited typical aggregation-induced emission charac-teristics owing to its twisted molecular conformation.Excellent biocompatibility and suitable lipophilicity allowed DPCMP to specifically stain the LDs in living cells.Under white light illumination,the DPCMP displayed potent reactive oxygen species(ROS)generation capacity through both type I and II photochemistry.The massive accumulation of lethal ROS generated by DPCMP-mediated photosensiti-zation initiated lipid peroxidation,impaired cellular redox homeostasis,and led to endoplasmic reticulum oxidative stress,ultimately inhibiting cellular proliferation via concurrent ferroptosis and apoptosis in both living cancer cells and multicellular tumor spheroids.展开更多
Activatable prodrugs have received considerable attention in cancer therapy due to their high specificity and reduced side effects.However,the theranostic prodrug with multiple cancerous organelles targeting and combi...Activatable prodrugs have received considerable attention in cancer therapy due to their high specificity and reduced side effects.However,the theranostic prodrug with multiple cancerous organelles targeting and combinational therapy is still rare.In this report,an esterase-responsive prodrug tetraphenylethylene functionalized quinolinium-ester-chlorambucil(TPEQC)was developed for dual organelles-targeted and image-guided cancer therapy through synergetic chemotherapy(CT)and photodynamic therapy(PDT).TPE-QC was constructed by conjugating an anticancer drug of chlorambucil with an aggregation-induced emission active photosensitizer of tetraphenylethylene functionalized hydroxyethyl quinolinium(TPE-QO)via the hydrolyzable ester linkage.The fluorescence and photosensitization of TPE-QC were initially quenched because of the photoinduced electron transfer(PET)effect.After reacting with esterase,the ester group of TPE-QC could be selectively hydrolyzed to release chlorambucil and TPE-QO,which terminated the PET process and switched on the fluorescence and photosensitization.Benefitting from the overexpressed esterase in cancer cells,TPE-QC could be efficiently activated in cancer cells rather than in normal cells,while the restoredfluorescence could preciselymonitor the release of TPE-QC.Importantly,activated TPE-QC accumulated in both organelles of lysosome and mitochondria,resulting in enhanced anticancer potency.In vivo experiments demonstrated that TPE-QC displayed efficient tumor microenvironment-activatable features and excellent tumor therapeutic effects through combinational CT and PDT.展开更多
基金National Natural Science Foundation of China,Grant/Award Numbers:22077077,22275119,21975149,21672135Fundamental Research Funds for the Central Universities,Grant/Award Numbers:GK202301010,18QNGG007,2021TS033。
文摘Lipid droplets(LDs),which are the hubs of lipid metabolism,play a critical role in maintaining cellular energy homeostasis.The construction of advanced photo-sensitizers(PSs)capable of manipulating LD-mediated cell fate regulation is highly desirable though rarely reported.In this study,a near-infrared emissive PS(DPCMP)with LDs specificity was synthesized and successfully applied to induce ferroptosis and apoptosis.DPCMP exhibited typical aggregation-induced emission charac-teristics owing to its twisted molecular conformation.Excellent biocompatibility and suitable lipophilicity allowed DPCMP to specifically stain the LDs in living cells.Under white light illumination,the DPCMP displayed potent reactive oxygen species(ROS)generation capacity through both type I and II photochemistry.The massive accumulation of lethal ROS generated by DPCMP-mediated photosensiti-zation initiated lipid peroxidation,impaired cellular redox homeostasis,and led to endoplasmic reticulum oxidative stress,ultimately inhibiting cellular proliferation via concurrent ferroptosis and apoptosis in both living cancer cells and multicellular tumor spheroids.
基金supported by National Natural Science Foundation of China(nos.21975149,22077077 and 21672135)Funded Projects for the Academic Leaders and Academic Backbones,Shaanxi Normal University(no.18QNGG007)the Fundamental Research Funds for the Central Universities(nos.GK201902006 and GK202003036).
文摘Activatable prodrugs have received considerable attention in cancer therapy due to their high specificity and reduced side effects.However,the theranostic prodrug with multiple cancerous organelles targeting and combinational therapy is still rare.In this report,an esterase-responsive prodrug tetraphenylethylene functionalized quinolinium-ester-chlorambucil(TPEQC)was developed for dual organelles-targeted and image-guided cancer therapy through synergetic chemotherapy(CT)and photodynamic therapy(PDT).TPE-QC was constructed by conjugating an anticancer drug of chlorambucil with an aggregation-induced emission active photosensitizer of tetraphenylethylene functionalized hydroxyethyl quinolinium(TPE-QO)via the hydrolyzable ester linkage.The fluorescence and photosensitization of TPE-QC were initially quenched because of the photoinduced electron transfer(PET)effect.After reacting with esterase,the ester group of TPE-QC could be selectively hydrolyzed to release chlorambucil and TPE-QO,which terminated the PET process and switched on the fluorescence and photosensitization.Benefitting from the overexpressed esterase in cancer cells,TPE-QC could be efficiently activated in cancer cells rather than in normal cells,while the restoredfluorescence could preciselymonitor the release of TPE-QC.Importantly,activated TPE-QC accumulated in both organelles of lysosome and mitochondria,resulting in enhanced anticancer potency.In vivo experiments demonstrated that TPE-QC displayed efficient tumor microenvironment-activatable features and excellent tumor therapeutic effects through combinational CT and PDT.
