The authors regret that the grant number“21CJ1402200”in the Acknowledgments session should be replaced as“21JC1402200”.The corrected contents areprovided as follows.
Grain size is an important determinant of grain weight and yield in rice.Although several genes related to grain size have been identified,natural variations in these genes that affect grain size are poorly characteri...Grain size is an important determinant of grain weight and yield in rice.Although several genes related to grain size have been identified,natural variations in these genes that affect grain size are poorly characterized.Here,we describe the grain length QTL GL10,encoding MADS56,which positively regulates grain length and grain weight.A natural allelic variation of NIL-gl10,containing an~1.0-kb deletion in the first exon that abolishes its transcription,results in shorter grain length,lower grain weight and delayed flowering in gl10 plants.The knockout of GL10 in the HJX74 background leads to grain phenotypes similar to that of NIL-gl10,while overexpression of GL10 results in increased grain length and weight and earlier heading date.GL10 regulates grain length by promoting greater longitudinal cell growth in the grain glume.Additionally,GL10 participates in the regulation of gibberellic acid(GA)signaling pathway genes in young panicle tissues.Analysis of GL10 haplotypes shows obvious divergence between the japonica and indica lineages.Our findings reveal an allelic variation of GL10 that may explain differences in grain length among modern cultivars and could be used to breed rice varieties with optimized grain shape.展开更多
In transonic wind tunnel tests,the pulsating airflow is prone to induce the first order resonance of the sting support system.The resonance limits the wind tunnel test envelope,makes the test data inaccurate,and bring...In transonic wind tunnel tests,the pulsating airflow is prone to induce the first order resonance of the sting support system.The resonance limits the wind tunnel test envelope,makes the test data inaccurate,and brings potential security risks.In this paper,a model support sting with constrained layer damping(CLD)treatment is proposed to reduce the first order resonance response.The CLD treatment mainly consists of material selection and geometric optimization processes.The damping performance of the optimized CLD sting is compared with an AISI 1045 steel sting with the identical diameter in laboratory.The frequency response curves of the CLD sting support system and the AISI 1045 steel sting support system are obtained by sine sweep tests.The test results show that the first order resonance response of the CLD sting support system is 37.3%of that of the AISI 1045 steel sting support system.The first order damping ratios are calculated from the frequency response curves by half power point method.It is found that the first order damping ratio of the CLD sting support system is approximately 2.6 times that of the AISI 1045 steel sting support system.展开更多
Significant chiroptical responses could be generated by chiral coupling of achiral plasmonic nanoparticles,or originated from intrinsically chiral plasmonic nanoparticles.Here we create dimeric plasmonic metamolecules...Significant chiroptical responses could be generated by chiral coupling of achiral plasmonic nanoparticles,or originated from intrinsically chiral plasmonic nanoparticles.Here we create dimeric plasmonic metamolecules possessing both chiral coupling between nanoparticles and intrinsic chiroptical responses derived from nanoparticles themselves.These plasmonic metamolecules are prepared by assembling helical plasmonic nanorods(HPNRs)with intrinsic chirality in chiral manners on DNA origami template.Two HPNRs with the same or opposite chirality,or one HPNR and one achiral gold nanorod,are coupled chirally into dimeric metamolecules with intriguing plasmonic circular dichroism(PCD).We found that both of the intrinsic chirality of constituent HPNRs and the chiral coupling contribute to the overall PCD while their weights are different in different metamolecules and vary in different wavelength range for a certain metamolecule.Comparing to conventional chiral plasmonic metamolecules from achiral nanoparticles,or discrete chiral nanoparticles,these metamolecules bring more dimensions for tailoring chiroptical responses and make it more flexible to design plasmonic nanodevices with custom PCD.展开更多
Background:Dopamine and dopamine receptor D1(DRD1),a member of the dopamine receptor family,have been indicated to play important roles in cancer progression,but dopamine secretion in hepatocellular carcinoma(HCC)and ...Background:Dopamine and dopamine receptor D1(DRD1),a member of the dopamine receptor family,have been indicated to play important roles in cancer progression,but dopamine secretion in hepatocellular carcinoma(HCC)and the effects of DRD1 on HCC remain unclear.This study was designed to explore the contribution of the dopaminergic system to HCC and determine the relationship between DRD1 and prognosis in HCC patients.Methods:The dopamine metabolic system was monitored using enzyme-linked immunosorbent assays(ELISAs).The expression of DRD1 was detected by microarray analysis,immunohistochemistry(IHC),and quantitative real-time PCR(qRT-PCR).Stable DRD1 knockout and overexpression cell lines were established for investigation.Transwell,colony formation,and Cell Counting Kit 8(CCK8)assays were performed to assess the malignant behaviors of cancer cells.The cAMP/PI3K/AKT/cAMP response element-binding(CREB)signaling pathway was evaluated by Western blot.This pathway,which is agitated by DRD1 in striatal neurons,had been proven to participate in tumor progression.Xenograft HCC tumors were generated for in vivo experiments.Results:Dopamine secretion increased locally in HCC due to an imbalance in dopamine metabolism,including the upregulation of dopa decarboxylase(DDC)and the downregulation of monoamine oxidase A(MAOA).Dopamine promoted the proliferation and metastasis of HCC.DRD1 was highly expressed in HCC tissues and positive DRD1 expression was related to a poor prognosis in HCC patients.The upregulation of DRD1 agitated malignant activities,including proliferation and metastasis in HCC by regulating the cAMP/PI3K/AKT/CREB pathway,and the downregulation of DRD1 had opposing effects.The effects of dopamine on HCC was reversed by depleting DRD1.SCH23390,a selective DRD1 antagonist,inhibited the proliferation and metastasis of HCC cells both in vitro and in vivo.Conclusion:Dopamine secretion was locally increased in HCC and promoted HCC cell proliferation and metastasis.DRD1 was found to exert positive effects on HCC progression and play a vital role in the dopamine system,and could be a potential therapeutic target and prognostic biomarker for HCC.展开更多
CRISPR/Cas9-mediated site-specific insertion of exogenous genes holds potential for clinical applications.However,it is still infeasible because homologous recombination(HR)is inefficient,especially for nondividing ce...CRISPR/Cas9-mediated site-specific insertion of exogenous genes holds potential for clinical applications.However,it is still infeasible because homologous recombination(HR)is inefficient,especially for nondividing cells.To overcome the challenge,we report that a homology-independent targeted integration(HITI)strategy is used for permanent integration of high-specificity-activity Factor IX variant(F9 Padua,R338L)at the albumin(Alb)locus in a novel hemophilia B(HB)rat model.The knock-in efficiency reaches 3.66%,as determined by droplet digital PCR(dd PCR).The clotting time is reduced to a normal level four weeks after treatment,and the circulating factor IX(FIX)level is gradually increased up to 52%of the normal level over nine months even after partial hepatectomy,demonstrating the amelioration of hemophilia.Through primer-extension-mediated sequencing(PEM-seq),no significant off-target effect is detected.This study not only provides a novel model for HB but also identifies a promising therapeutic approach for rare inherited diseases.展开更多
基金National Key R&D Program of China(2019YFA0110802 and 2019YFA0802800)the National Natural Science Foundation of China(32025023,31971366)+1 种基金the Shanghai Municipal Commission for Science and Technology(21JC1402200,20140900200)the Innovation Program of Shanghai Municipal Education Commission(2019-01-07-00-05-E00054)。
文摘The authors regret that the grant number“21CJ1402200”in the Acknowledgments session should be replaced as“21JC1402200”.The corrected contents areprovided as follows.
基金supported by the National Natural Science Foundation of China (32072040, 31622041)the Key Projects of Basic Research and Applied Basic Research of Guangdong Province(2019B030302006)
文摘Grain size is an important determinant of grain weight and yield in rice.Although several genes related to grain size have been identified,natural variations in these genes that affect grain size are poorly characterized.Here,we describe the grain length QTL GL10,encoding MADS56,which positively regulates grain length and grain weight.A natural allelic variation of NIL-gl10,containing an~1.0-kb deletion in the first exon that abolishes its transcription,results in shorter grain length,lower grain weight and delayed flowering in gl10 plants.The knockout of GL10 in the HJX74 background leads to grain phenotypes similar to that of NIL-gl10,while overexpression of GL10 results in increased grain length and weight and earlier heading date.GL10 regulates grain length by promoting greater longitudinal cell growth in the grain glume.Additionally,GL10 participates in the regulation of gibberellic acid(GA)signaling pathway genes in young panicle tissues.Analysis of GL10 haplotypes shows obvious divergence between the japonica and indica lineages.Our findings reveal an allelic variation of GL10 that may explain differences in grain length among modern cultivars and could be used to breed rice varieties with optimized grain shape.
基金supported by Fenglei Youth Innovation Fund of China Aerodynamics Research&Development Center(PJD20180189)Shandong Provincial Natural Science Foundation of China(2019JMRH0307)supported by grants from Shandong University and Taishan Scholar Foundation。
文摘In transonic wind tunnel tests,the pulsating airflow is prone to induce the first order resonance of the sting support system.The resonance limits the wind tunnel test envelope,makes the test data inaccurate,and brings potential security risks.In this paper,a model support sting with constrained layer damping(CLD)treatment is proposed to reduce the first order resonance response.The CLD treatment mainly consists of material selection and geometric optimization processes.The damping performance of the optimized CLD sting is compared with an AISI 1045 steel sting with the identical diameter in laboratory.The frequency response curves of the CLD sting support system and the AISI 1045 steel sting support system are obtained by sine sweep tests.The test results show that the first order resonance response of the CLD sting support system is 37.3%of that of the AISI 1045 steel sting support system.The first order damping ratios are calculated from the frequency response curves by half power point method.It is found that the first order damping ratio of the CLD sting support system is approximately 2.6 times that of the AISI 1045 steel sting support system.
基金supported by the National Natural Science Foundation of China(Nos.21977112,and 21934007)the Natural Science Foundation of Jiangsu Province(No.BK20190227)+1 种基金the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000)the Science and Technology Project of Suzhou(No.SZS201904).
文摘Significant chiroptical responses could be generated by chiral coupling of achiral plasmonic nanoparticles,or originated from intrinsically chiral plasmonic nanoparticles.Here we create dimeric plasmonic metamolecules possessing both chiral coupling between nanoparticles and intrinsic chiroptical responses derived from nanoparticles themselves.These plasmonic metamolecules are prepared by assembling helical plasmonic nanorods(HPNRs)with intrinsic chirality in chiral manners on DNA origami template.Two HPNRs with the same or opposite chirality,or one HPNR and one achiral gold nanorod,are coupled chirally into dimeric metamolecules with intriguing plasmonic circular dichroism(PCD).We found that both of the intrinsic chirality of constituent HPNRs and the chiral coupling contribute to the overall PCD while their weights are different in different metamolecules and vary in different wavelength range for a certain metamolecule.Comparing to conventional chiral plasmonic metamolecules from achiral nanoparticles,or discrete chiral nanoparticles,these metamolecules bring more dimensions for tailoring chiroptical responses and make it more flexible to design plasmonic nanodevices with custom PCD.
基金This work was supported by grants from the National Natural Science Foundation of China(grant 81971057 to WAZ and grant 81902490 to DTC).
文摘Background:Dopamine and dopamine receptor D1(DRD1),a member of the dopamine receptor family,have been indicated to play important roles in cancer progression,but dopamine secretion in hepatocellular carcinoma(HCC)and the effects of DRD1 on HCC remain unclear.This study was designed to explore the contribution of the dopaminergic system to HCC and determine the relationship between DRD1 and prognosis in HCC patients.Methods:The dopamine metabolic system was monitored using enzyme-linked immunosorbent assays(ELISAs).The expression of DRD1 was detected by microarray analysis,immunohistochemistry(IHC),and quantitative real-time PCR(qRT-PCR).Stable DRD1 knockout and overexpression cell lines were established for investigation.Transwell,colony formation,and Cell Counting Kit 8(CCK8)assays were performed to assess the malignant behaviors of cancer cells.The cAMP/PI3K/AKT/cAMP response element-binding(CREB)signaling pathway was evaluated by Western blot.This pathway,which is agitated by DRD1 in striatal neurons,had been proven to participate in tumor progression.Xenograft HCC tumors were generated for in vivo experiments.Results:Dopamine secretion increased locally in HCC due to an imbalance in dopamine metabolism,including the upregulation of dopa decarboxylase(DDC)and the downregulation of monoamine oxidase A(MAOA).Dopamine promoted the proliferation and metastasis of HCC.DRD1 was highly expressed in HCC tissues and positive DRD1 expression was related to a poor prognosis in HCC patients.The upregulation of DRD1 agitated malignant activities,including proliferation and metastasis in HCC by regulating the cAMP/PI3K/AKT/CREB pathway,and the downregulation of DRD1 had opposing effects.The effects of dopamine on HCC was reversed by depleting DRD1.SCH23390,a selective DRD1 antagonist,inhibited the proliferation and metastasis of HCC cells both in vitro and in vivo.Conclusion:Dopamine secretion was locally increased in HCC and promoted HCC cell proliferation and metastasis.DRD1 was found to exert positive effects on HCC progression and play a vital role in the dopamine system,and could be a potential therapeutic target and prognostic biomarker for HCC.
基金supported by grants from National Key R&D Program of China(2019YFA0110802 and 2019YFA0802800)the National Natural Science Foundation of China(32025023,31971366)+1 种基金grants from the Shanghai Municipal Commission for Science and Technology(21CJ1402200,20140900200)the Innovation Program of Shanghai Municipal Education Commission(2019-01-07-00-05-E00054)。
文摘CRISPR/Cas9-mediated site-specific insertion of exogenous genes holds potential for clinical applications.However,it is still infeasible because homologous recombination(HR)is inefficient,especially for nondividing cells.To overcome the challenge,we report that a homology-independent targeted integration(HITI)strategy is used for permanent integration of high-specificity-activity Factor IX variant(F9 Padua,R338L)at the albumin(Alb)locus in a novel hemophilia B(HB)rat model.The knock-in efficiency reaches 3.66%,as determined by droplet digital PCR(dd PCR).The clotting time is reduced to a normal level four weeks after treatment,and the circulating factor IX(FIX)level is gradually increased up to 52%of the normal level over nine months even after partial hepatectomy,demonstrating the amelioration of hemophilia.Through primer-extension-mediated sequencing(PEM-seq),no significant off-target effect is detected.This study not only provides a novel model for HB but also identifies a promising therapeutic approach for rare inherited diseases.