Lithium (Li) metal is considered as one of the most promising anode materials to build next-generation high-energy–density batteries. Nonetheless, dendritic Li deposition has dramatically hindered the practical appli...Lithium (Li) metal is considered as one of the most promising anode materials to build next-generation high-energy–density batteries. Nonetheless, dendritic Li deposition has dramatically hindered the practical applications of Li metal batteries (LMBs). Uniformizing Li deposition is a prerequisite to achieve safe and practical LMBs. Herein, an underpotential deposition (UPD) process is first proposed to alter the kinetic and uniformity of Li deposition morphology. Based on the strong interaction between the Li adatoms and manganese (Mn) based substrate, a competition between the UPD and bulk Li deposition is observed, on which the predominance of the UPD scenario tends to uniformize Li nucleation and deposition by the surface coverage of Li monolayers at potentials that are more positive than the Nernst potential of Li metal. Inspired by this process, an advanced hybrid Mn-graphene oxide structure is developed for Li protection, not only enabling dendrite-free Li anodes for high-capacity and -current density cycling, but also improving the interfacial kinetic of Li metal anodes at subzero temperatures, showing potential applicability in low temperature conditions.展开更多
We have shown that platelet-derived microvesicles(PMVs)induce abnormal proliferation,migration,and energy metabolism of vascular smooth muscle cells(VSMCs)after vascular intimal injury.Here,we examined a novel role of...We have shown that platelet-derived microvesicles(PMVs)induce abnormal proliferation,migration,and energy metabolism of vascular smooth muscle cells(VSMCs)after vascular intimal injury.Here,we examined a novel role of podosome in mediating matrix metalloproteinase-9(MMP-9)dependent VSMC migration induced by plateletderived microvesicles(PMVs).VSMCs were isolated from the thoracic aortas of male Sprague Dawley(SD)rats and identified with immunofluorescent staining.Blood samples were collected from SD Rats,the platelets were isolated with density gradient centrifugation from the blood samples and activated by collagen I.Intriguingly,proteins expressed in platelets were found to participate in the positive regulation of podosome assembly using GO analysis by DAVID,and most of the proteins were found in extracellular exosomes.Of note,activated platelets indirectly induced VSMC migration via releasing PMVs which was verified using platelets and VSMCs transwell coculture system.Besides,podosome,an invasive protrusion to mediate extracellular matrix(ECM)remodeling,was formed in VSMCs to induce cell migration.Furthermore,MMP-9 activity detected by gelatin zymography was used to verify the function of the podosome in ECM remodeling.The result indicated that MMP-9 activity was robustly activated in VSMCs to implement the function of the podosome.In addition,gelatin degradation was detected in intact VSMCs using a gelatin degradation assay after co-culture with platelets.Taken together,our data reveal a novel mechanism that PMVs promote VSMC migration via forming podosomes and inducing MMP-9 activity.展开更多
基金This work was supported by the 21C Innovation Laboratory,Contemporary Amperex Technology Ltd by project No.21C-OP-202004the Ningbo 2025 Project(Grant No.2018B10061,2018B10087 and 2019B10044)+1 种基金the National Key R&D Program of China(Grant No.2018YFB0905400)the National Natural Science Foundation of China(Grant No.21805297).
文摘Lithium (Li) metal is considered as one of the most promising anode materials to build next-generation high-energy–density batteries. Nonetheless, dendritic Li deposition has dramatically hindered the practical applications of Li metal batteries (LMBs). Uniformizing Li deposition is a prerequisite to achieve safe and practical LMBs. Herein, an underpotential deposition (UPD) process is first proposed to alter the kinetic and uniformity of Li deposition morphology. Based on the strong interaction between the Li adatoms and manganese (Mn) based substrate, a competition between the UPD and bulk Li deposition is observed, on which the predominance of the UPD scenario tends to uniformize Li nucleation and deposition by the surface coverage of Li monolayers at potentials that are more positive than the Nernst potential of Li metal. Inspired by this process, an advanced hybrid Mn-graphene oxide structure is developed for Li protection, not only enabling dendrite-free Li anodes for high-capacity and -current density cycling, but also improving the interfacial kinetic of Li metal anodes at subzero temperatures, showing potential applicability in low temperature conditions.
基金supported by grants from the National Natural Science Foundation of China[grant numbers 12032003 and 12102261].
文摘We have shown that platelet-derived microvesicles(PMVs)induce abnormal proliferation,migration,and energy metabolism of vascular smooth muscle cells(VSMCs)after vascular intimal injury.Here,we examined a novel role of podosome in mediating matrix metalloproteinase-9(MMP-9)dependent VSMC migration induced by plateletderived microvesicles(PMVs).VSMCs were isolated from the thoracic aortas of male Sprague Dawley(SD)rats and identified with immunofluorescent staining.Blood samples were collected from SD Rats,the platelets were isolated with density gradient centrifugation from the blood samples and activated by collagen I.Intriguingly,proteins expressed in platelets were found to participate in the positive regulation of podosome assembly using GO analysis by DAVID,and most of the proteins were found in extracellular exosomes.Of note,activated platelets indirectly induced VSMC migration via releasing PMVs which was verified using platelets and VSMCs transwell coculture system.Besides,podosome,an invasive protrusion to mediate extracellular matrix(ECM)remodeling,was formed in VSMCs to induce cell migration.Furthermore,MMP-9 activity detected by gelatin zymography was used to verify the function of the podosome in ECM remodeling.The result indicated that MMP-9 activity was robustly activated in VSMCs to implement the function of the podosome.In addition,gelatin degradation was detected in intact VSMCs using a gelatin degradation assay after co-culture with platelets.Taken together,our data reveal a novel mechanism that PMVs promote VSMC migration via forming podosomes and inducing MMP-9 activity.
