Hematopoietic stem and progenitor cells(HSPCs)are able to self-renew and can give rise to all blood lineages throughout their lifetime,yet the mechanisms regulating HSPC development have yet to be discovered.In this s...Hematopoietic stem and progenitor cells(HSPCs)are able to self-renew and can give rise to all blood lineages throughout their lifetime,yet the mechanisms regulating HSPC development have yet to be discovered.In this study,we characterized a hematopoiesis defective zebrafish mutant line named smu07,which was obtained from our previous forward genetic screening,and found the HSPC expansion deficiency in the mutant.Positional cloning identified that slc20a1b,which encodes a sodium phosphate cotransporter,contributed to the smu07 blood phenotype.Further analysis demonstrated that mutation of slc20a1b affects HSPC expansion through cell cycle arrest at G2/M phases in a cell-autonomous manner.Our study shows that slc20a1b is a vital regulator for HSPC proliferation in zebrafish early hematopoiesis and provides valuable insights into HSPC development.展开更多
基金supported by the National Key Research and Development Program of China(2018YFA0800200)the National Natural Science Foundation of China(31922023)+2 种基金China Postdoctoral Science Foundation(2018M643071)Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme(2019)the Fundamental Research Funds for the Central Universities(2019ZD54 and 2019MS131)。
文摘Hematopoietic stem and progenitor cells(HSPCs)are able to self-renew and can give rise to all blood lineages throughout their lifetime,yet the mechanisms regulating HSPC development have yet to be discovered.In this study,we characterized a hematopoiesis defective zebrafish mutant line named smu07,which was obtained from our previous forward genetic screening,and found the HSPC expansion deficiency in the mutant.Positional cloning identified that slc20a1b,which encodes a sodium phosphate cotransporter,contributed to the smu07 blood phenotype.Further analysis demonstrated that mutation of slc20a1b affects HSPC expansion through cell cycle arrest at G2/M phases in a cell-autonomous manner.Our study shows that slc20a1b is a vital regulator for HSPC proliferation in zebrafish early hematopoiesis and provides valuable insights into HSPC development.
