Chinese family care patterns of childhood rheumatic diseases:A cluster analysis

Objectives:The purpose is to distinguish family care(FC)patterns of childhood rheumatic diseases in Chinese families and to determine the predictors of FC patterns.Methods:This secondary analysis contained two cross-section surveys with a convenient sample of totally 398 caregivers who have a child with rheumatic diseases from four pediatric hospitals.Caregivers were required to completed Family Management Measure questionnaire.Cluster analysis was used to distinguish patterns and multinomial logistic regression analysis was used to find predictors.Results:Four patterns were identified:the normal-perspective and collaborative(28.4%),the effortless and contradictory(24.6%),the chaotic and strenuous(18.3%),and the confident and concerning(28.7%).Disease category(x2=21.23,P=0.002),geographic location(x2=8.41,P=0,038),maternal educational level(x2=12.69,P=0.048)and family monthly income(x2=33.21,P<0.001)predicted different patterns.Conclusions:FC patterns were different among families.Disease-related and family-related factors were vital predictors to distinguish patterns consistent with the Family Management Style Framework.The result assisted that clinicians recognize FC patterns and predictors effectively to provide tailored advice in time.

Summer Extreme Precipitation in the Key Region of the Sichuan-Tibet Railway

The Sichuan-Tibet Railway,mainly located in the southeastern Qinghai-Tibet Plateau,is affected by summertime extreme precipitation(SEP).Using daily rain-gauge observations and ERA5 reanalysis data for the summers of 1979-2020,the spatiotemporal distribution characteristics of SEP in the key region of the Sichuan-Tibet Railway(28°-33°N,90°-105°E,hereafter KR)are revealed,and the mechanism for SEP amount(SEPA)variation in the KR is investigated.The results show that SEPA in the KR contributes nearly 30%to the total summer precipitation.Regional differences are evident in SEP,justifying thresholds higher in the plateau-dominated central-western KR(CWKR)and lower in the basindominated eastern KR(EKR).In addition,SEP in the CWKR is less intense but more frequent than SEP in the EKR.During 1979-2020,the SEPA in the KR increased slightly while the SEPA in the CWKR increased significantly and peaked in the last decade.When anticyclonic circulation(AC)anomalies dominate the 500 hPa pattern over the Bay of Bengal and Mongolia,the southerly flow and cyclonic shear over the southeastern plateau will be strengthened,favoring more SEPA in the CWKR.When an AC anomaly dominates the 500 hPa pattern over the Bohai Sea,the low-level easterly wind over the basin will be strengthened,favoring more SEPA in the EKR.The strengthening of the ascent,water vapor convergence,and convective instability is conducive to more SEPA in the KR.Our results deepen the understanding of the characteristics and the physical mechanisms responsible for extreme precipitation in the KR.

Comparison of an automated digital cell morphology analysis system with manual counting

Background:Bionovation's CSFA800 is a new automated digital cell imaging analyzer.We evaluated the performance of the CSFA800 by comparing it with artificial peripheral blood white blood cell counting.Methods:According to inclusion and exclusion criteria,131 randomly selected samples(77 abnormal samples and 54 normal samples)were compared.Correlations between automated and manual counting results were analyzed.Manual counting was carried out according to the guidelines of the Association of Clinical and Laboratory Standards.Results:Counts of neutrophils,lymphocytes,monocytes,eosinophils,baso-phils,and immature granulocytes obtained from CSFA800 and artificial methods were linearly and positively correlated,with R values of 0.73,0.65,0.24,0.2,0.4,and 0.63,respectively,all p<0.05.Therefore,correlations be-tween CSFA800 and manual counting are acceptable.Compared with the DI‐60 Automated Digital Cell Morphology System(DI‐60;Sysmex),CSFA800 is more efficient and can analyze 20,000 cells in 1 min.However,the overall accuracy of CSFA800 is not as good as DI‐60,although its counting perfor-mance is better for basophils.Conclusions:The performance of CSFA800 for WBC counts is acceptable,and it displayed good performance for neutrophils,lymphocytes,and imma-ture granulocytes.Compared to DI‐60,CSFA800 is more efficient but has slightly lower overall accuracy.To some extent,CSFA800 is helpful to opti-mize the clinical laboratory workflow and improve the working efficiency of inspectors.

An iASPP-derived short peptide restores p53-mediated cell death in cancers with wild-type p53

Background:Inhibitor of apoptosis-stimulating protein of p53(iASPP)is an evolutionarily conserved p53 inhibitor.Mechanistically,iASPP can accelerate tumorigenesis by inhibiting the transactivation function of p53.Targeting the interaction between iASPP and p53 may be a potential therapy for restoring the activity of p53 in tumors.Methods:We constructed an iASPP-derived peptide,called A8,that was derived from the C-terminus of iASPP.Here,we transfected A8 into two wildtype(WT)p53 cell lines,U2OS and A549,and then determined the number of apoptotic cells.The mechanism by which A8 affected apoptosis was further examined by immunoprecipitation(IP),Dual-Luciferase reporter assays,and chromatin IP assays.Real-time polymerase chain reaction and western blots were also used to examine the expression levels of apoptosis-related factors.Results:Our data demonstrate that A8 can increase apoptosis rates in WT p53 cell lines.Functional analysis suggested that A8 restored the transcriptional function and DNA binding activities of p53 toward the Bax and PUMA gene promoters.Moreover,A8 reduced cell proliferation and inhibited tumor growth in xenograft nude mice.Conclusions:These data provide a new approach for restoring the tumor suppressor function of p53 in cancer cells that express WT p53 and therefore may serve as a novel cancer treatment strategy.