Bioinformatics analysis of structure and function in the MRP gene family and its expression in response to various drugs in Bursaphelenchus xylophilus

Genes homologous to members of the MRP gene family in Caenorhabditis elegans are important in drug resistance.To further explore the molecular mechanism of drug resistance in pine wood nematode(Bursaphelenchus xylophilus),we used bioinformatics approaches to analyze genomic data for B.xylophilus and identified Bx-MRP genes.We predicted the structure and function of the genes and encoded proteins.Using bioinformatics programs to predict and analyze various properties of the predicted proteins,including hydrophobicity,transmembrane regions,phosphorylation sites,and topologically isomeric structures,of these Bx-MRP genes,we determined that they function in transmembrane transport.From the results of RT-qPCR,the Bx-MRP family members confer significant differential resistance to different drug treatments.After treatment with different concentrations of emamectin benzoate,avermectin and matrine,the expression of each gene increased with increasing drug concentrations,indicating that the family members play a positive role in the regulation of multidrug resistance.

Overexpression of the PdpapERF109 gene enhances resistance of Populus davidiana×P.alba var.pyramidalis to Fusarium oxysporum infection

The key transcription factor gene PdP apE RF109 was cloned from Populus davidiana×P.alba var.pyramidalis(Pdpap),and after overexpression of P dP ap ERF109 in transformants,the gene functions in the resistance response to Fusarium oxysporum infection.Compared with the wild Pdpap,after inoculation with F.oxysporum,the physiological and biochemical characteristics,including relative fresh weight,peroxidase activity,and the percentage of electrolyte leakage showed that,after overexpression of the PdPapERF109 gene,the transformants grew well and displayed significant resistance to F.oxysporum infection.By comparing the reactive oxygen species scavenging capacity of Pdpap plants after pathogen infection,the P dPapERF109-overexpressing plants had significantly better reactive oxygen species scavenging ability than the wild plants.Comprehensive analysis of plant morphology and various physiological and biochemical parameters showed that the overexpression of the P dpapERF109 gene significantly improved the resistance of Pdpap plants to F.oxysporum root rot.Therefore,increasing the expression of the homologous ERF109 gene can be an effective strategy to increase disease resistance in hybrid poplars.