Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient co...Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient conditions to help plants cope with recurring environmental fluctuations.Despite the current understanding of plant circadian clock and genetic framework underlying plant shoot architecture,the intricate connection between these two adaptive mechanisms remains largely unclear.In this study,we elucidated how the core clock gene LUX ARRHYTHMO(LUX)regulates shoot architecture in the model legume plant Medicago truncatula.We show that mtlux mutant displays increased main stem height,reduced lateral shoot length,and decreased the number of lateral branches and biomass yield.Gene expression analysis revealed that Mt LUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A,a TCP gene that functions centrally in modulating shoot architecture.In vivo and in vitro experiments showed that Mt LUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A,suggesting that Mt LUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.This work demonstrates the regulatory effect of the circadian clock on shoot architecture,offering a new understanding underlying the genetic basis towards the flexibility of plant shoot architecture.展开更多
This meta-analysis of randomized controlled trials aimed to evaluate the effects of probiotic supplementation on glucose homeostasis in patients with polycystic ovary syndrome(PCOS).The meta-analysis was performed in ...This meta-analysis of randomized controlled trials aimed to evaluate the effects of probiotic supplementation on glucose homeostasis in patients with polycystic ovary syndrome(PCOS).The meta-analysis was performed in accordance with the Cochrane Handbook guidelines and relevant the preferred reporting items for systematic reviews and meta-analyses(PRISMA)statement criteria.Of 825 identified reports,11 randomized clinical trials were included in the meta-analysis.An analysis of pooled extracted data revealed that supplementation with probiotics significantly decreased fasting blood glucose(FBG,n=7;standardized mean difference(SMD)=−0.40;95%confidence interval(CI):−2.02,−0.02;P=0.04)and insulin levels(n=6;SMD=−0.57;95%CI:−0.89,−0.25;P=0.0004)and the homeostatic model assessment of insulin resistance(n=7;SMD=−0.64;95%CI:−0.96,−0.31;P=0.0001)while increasing the quantitative insulin sensitivity check index(QUICKI,n=5;SMD=0.58;95%CI:0.08,1.09;P=0.02)in patients with PCOS.The FBG-reducing effect decreased as the baseline body mass index(BMI)and mean age of the participants increased.Indeed,a greater number of bacterial species and a higher bacterial dose were shown to reduce QUICKI effectively.The systematic review indicated that probiotic supplementation may help to control glucose homeostasis in adults with polycystic ovarian syndrome.展开更多
Senescence impairs preosteoblast expansion and differentiation into functional osteoblasts,blunts their responses to bone formation-stimulating factors and stimulates their secretion of osteoclast-activating factors.D...Senescence impairs preosteoblast expansion and differentiation into functional osteoblasts,blunts their responses to bone formation-stimulating factors and stimulates their secretion of osteoclast-activating factors.Due to these adverse effects,preosteoblast senescence is a crucial target for the treatment of age-related bone loss;however,the underlying mechanism remains unclear.We found that mTORC1 accelerated preosteoblast senescence in vitro and in a mouse model.Mechanistically,mTORC1 induced a change in the membrane potential from polarization to depolarization,thus promoting cell senescence by increasing Ca^(2+)influx and activating downstream NFAT/ATF3/p53 signaling.We further identified the sodium channel Scn1a as a mediator of membrane depolarization in senescent preosteoblasts.Scn1a expression was found to be positively regulated by mTORC1 upstream of C/EBPα,whereas its permeability to Na^(+)was found to be gated by protein kinase A(PKA)-induced phosphorylation.Prosenescent stresses increased the permeability of Scn1a to Na^(+)by suppressing PKA activity and induced depolarization in preosteoblasts.Together,our findings identify a novel pathway involving mTORC1,Scn1a expression and gating,plasma membrane depolarization,increased Ca^(2+)influx and NFAT/ATF3/p53 signaling in the regulation of preosteoblast senescence.Pharmaceutical studies of the related pathways and agents might lead to novel potential treatments for agerelated bone loss.展开更多
JF305 is a highly prolific pancreatic cancer cell line that originated from a Chinese patient. The cell line bears a functional HR double strand DNA repair mechanism but very responsive to PARP treatment a phenomenon ...JF305 is a highly prolific pancreatic cancer cell line that originated from a Chinese patient. The cell line bears a functional HR double strand DNA repair mechanism but very responsive to PARP treatment a phenomenon clearly suggesting presence of an anomaly in the mechanism. Brca1, Brca2 and CHK2 proteins are very important constituents of the HR mechanism whose respective gene coding mutations are strongly associated with several cancers and are widely exploited in anticancer chemotherapy. In this current study, the BRCA1, BRCA2 gene mutation status in JF305 was determined together with the presence of 3 widely reported cancer linked CHK2 founder mutations (1100delC, I157T, IVS2 +IG > A). CHK21100delC genotype was determined using allele specific PCR, while the PCR-RFLP assay was used for I157T, IVS2 +IG > A analysis. PCR and direct sequencing were used for assessing the BRCA1 and BRCA2 gene. Results revealed that JF305 is CHK21100delC heterozygous mutant, CHK2I157T and CHK2IVS2 +IG > A wild type. Furthermore, it was observed that JF305 lacked BRCA1 and BRCA2 gene mutations. The mutation status identification of CHK2 and BRCA1/2 in JF305 provides a major milestone towards elucidating the properties of the cell line which subsequently promises to be an excellent model for evaluating the role of parp inhibitors in pancreatic cancer chemotherapy most especially in the respective cancer cell lines without BRCA1 and BRCA2 gene mutations.展开更多
Background: Gemcitabine is a deoxycytidine analog, which is used as first-line agent for pancreatic cancer therapy, and its efficacy relied on its intracellular conversion to active triphosphate form. However, adminis...Background: Gemcitabine is a deoxycytidine analog, which is used as first-line agent for pancreatic cancer therapy, and its efficacy relied on its intracellular conversion to active triphosphate form. However, administration with gemcitabine still has limited effect on the overall survival of patients with pancreatic cancer. Objective: We aimed to study the combination effect of gemcitabine and doxorubicin to pancreatic cancer cells BxPC3 and PANC1, and unveil the mechanism. Methods: The study was performed in pancreatic cancer cells PANC1 and BxPC3, the contribution of UMP/CMP kinase 1 (CMPK1) to gemcitabine in PANC1 and BxPC3 cells was measured by transfection of CMPK1 plasmid or CMPK1 siRNA treatment to adjust the expression of CMPK1 in the cells;then analyzed the cell vitality and migration after treated with 1% IC50 of doxorubicin and gemcitabine or only with gemcitabine;the activity of CMPK1 and the effect of doxorubicin to the reaction was measured by HPLC assay in vitro;at last, docking analysis by computer was used to calculate the possible interaction sites of CMPK1 to DOX. Results: The sensitivity of PANC1 and BxPC3 cells to gemcitabine was improved when increasing the expression of CMPK1, and decreased when knockout CMPK1 by CMPK1 siRNA in BxPC3 cells;when combined with doxorubicin, the sensitivity of PANC1 and BxPC3 cells to gemcitabine also increased, and the cells migration reduced;we further found out that by adding 10 μM doxorubicin, the catalyzing activity of CMPK1 elevated about 2 times in vitro;the docking result showed that the association of CMPK1 to DOX was mainly by hydrogen bond and ionic interaction. Conclusion: CMPK1 can catalyze gemcitabine to its active form within the cells so that the sensitivity of the cells to gemcitabine elevated, and doxorubicin may enhance the cytotoxic effect to pancreatic cancer by up-regulate the activity of CMPK1, the combination of these deoxycytidine analogs with DOX might exert better efficacy.展开更多
Membrane technology has widely accepted as the most energy efficient technology for the separation of ions or molecules. As the core of the membrane technology, the chemical and structure nature of membrane materials ...Membrane technology has widely accepted as the most energy efficient technology for the separation of ions or molecules. As the core of the membrane technology, the chemical and structure nature of membrane materials determines the application and energy-efficiency of the technology. An advanced membrane that has a remarkable ability to selectively differentiate ions and molecules can significantly enhance the separation technology and revolutionize industrial separation processes.展开更多
CO 2 is a main component of gases in the Yangbajing geothermal field, usually higher than 85 in volume; others are N 2, H 2S, H 2, CH 4, etc. Helium R/R a ratios, ranging from 0.14 to 0.46, indicate a crustal componen...CO 2 is a main component of gases in the Yangbajing geothermal field, usually higher than 85 in volume; others are N 2, H 2S, H 2, CH 4, etc. Helium R/R a ratios, ranging from 0.14 to 0.46, indicate a crustal component to be dominant in the gases. δ 13C-CO 2 values and δ 34S-H 2S values are in the range -7.72‰--11.33‰ and 0.2‰-8.3‰, respectively. The gases are inferred to mostly derive from the partial melting of the Nyainqentanglha core complex. The gases of shallow and deep reservoirs are distinctive in chemical compositions and δ 34S-H 2S values, which shows their different flowing paths and physical-chemical processes. Sulphur does not approach isotopic equilibrium between H 2S and SO 2- 4 species in both reservoirs. Significance of steam chemical monitoring is also discussed.展开更多
Plant architecture and panicle architecture are two critical agronomic traits that greatly affect the yield of rice(Oryza sativa).PROSTRATE GROWTH 1(PROG1)encodes a key C2H2-type zinc-finger transcription factor and h...Plant architecture and panicle architecture are two critical agronomic traits that greatly affect the yield of rice(Oryza sativa).PROSTRATE GROWTH 1(PROG1)encodes a key C2H2-type zinc-finger transcription factor and has pleiotropic effects on the regulation of both plant and panicle architecture,thereby influencing the grain yield.However,the molecular mechanisms through which PROG1 controls plant and panicle architecture remain unclear.In this study,we showed that PROG1 directly binds the LAZY 1(LA1)promoter and acts as a repressor to inhibit LA1 expression.Conversely,LA1 acts as a repressor of PROG1 by directly binding to the PROG1 promoter.These two genes play antagonistic roles in shaping plant architecture by regulating both tiller angle and tiller number.Interestingly,our data showed that PROG1 controls panicle architecture through direct binding to the intragenic regulatory regions of OsGIGANTEA(OsGI)and subsequent activation of its expression.Collectively,we have identified two crucial targets of PROG1,LA1 and OsGI,shedding light on the molecular mechanisms underlying plant and panicle architecture control by PROG1.Our study provides valuable insights into the regulation of key domestication-related traits in rice and identifies potential targets for future high-yield rice breeding.展开更多
Diseases caused by invasive pathogens are an increasing threat to forest health,and early and accurate disease detection is essential for timely and precision forest management.The recent technological advancements in...Diseases caused by invasive pathogens are an increasing threat to forest health,and early and accurate disease detection is essential for timely and precision forest management.The recent technological advancements in spectral imaging and artificial intelligence have opened up new possibilities for plant disease detection in both crops and trees.In this study,Dutch elm disease(DED;caused by Ophiostoma novo-ulmi,)and American elm(Ulmus americana)was used as example pathosystem to evaluate the accuracy of two in-house developed high-precision portable hyper-and multi-spectral leaf imagers combined with machine learning as new tools for forest disease detection.Hyper-and multi-spectral images were collected from leaves of American elm geno-types with varied disease susceptibilities after mock-inoculation and inoculation with O.novo-ulmi under green-house conditions.Both traditional machine learning and state-of-art deep learning models were built upon derived spectra and directly upon spectral image cubes.Deep learning models that incorporate both spectral and spatial features of high-resolution spectral leaf images have better performance than traditional machine learning models built upon spectral features alone in detecting DED.Edges and symptomatic spots on the leaves were highlighted in the deep learning model as important spatial features to distinguish leaves from inoculated and mock-inoculated trees.In addition,spectral and spatial feature patterns identified in the machine learning-based models were found relative to the DED susceptibility of elm genotypes.Though further studies are needed to assess applications in other pathosystems,hyper-and multi-spectral leaf imagers combined with machine learning show potential as new tools for disease phenotyping in trees.展开更多
Currently,the presence of genetically modified(GM)organisms in agro-food markets is strictly regulated by enacted legislation worldwide.It is essential to ensure the traceability of these transgenic products for food ...Currently,the presence of genetically modified(GM)organisms in agro-food markets is strictly regulated by enacted legislation worldwide.It is essential to ensure the traceability of these transgenic products for food safety,consumer choice,environmental monitoring,market integrity,and scientific research.However,detecting the existence of GM organisms involves a combination of complex,time-consuming,and labor-intensive techniques requiring high-level professional skills.In this paper,a concise and rapid pipeline method to identify transgenic rice seeds was proposed on the basis of spectral imaging technologies and the deep learning approach.The composition of metabolome across 3 rice seed lines containing the cry1Ab/cry1Ac gene was compared and studied,substantiating the intrinsic variability induced by these GM traits.Results showed that near-infrared and terahertz spectra from different genotypes could reveal the regularity of GM metabolic variation.The established cascade deep learning model divided GM discrimination into 2 phases including variety classification and GM status identification.It could be found that terahertz absorption spectra contained more valuable features and achieved the highest accuracy of 97.04%for variety classification and 99.71%for GM status identification.Moreover,a modified guided backpropagation algorithm was proposed to select the task-specific characteristic wavelengths for further reducing the redundancy of the original spectra.The experimental validation of the cascade discriminant method in conjunction with spectroscopy confirmed its viability,simplicity,and effectiveness as a valuable tool for the detection of GM rice seeds.This approach also demonstrated its great potential in distilling crucial features for expedited transgenic risk assessment.展开更多
Medicinal plants have aroused considerable interest as an alternative to chemical drugs due to the beneficial effects of their active secondary metabolites.However,the extensive use of chemical fertilizers and pesticid...Medicinal plants have aroused considerable interest as an alternative to chemical drugs due to the beneficial effects of their active secondary metabolites.However,the extensive use of chemical fertilizers and pesticides in pursuit of yield has caused serious pollution to the environment,which is not conducive to sustainable devel-opment in thefield of medicinal plants.Microbial fertilizers are a type of“green fertilizer”containing specific microorganisms that can improve the soil microbial structure,enhance plant resistance to biological and abiotic stresses,and increase the yield of medicinal plants.The root exudates of medicinal plants attract different micro-organisms to the rhizosphere,which then migrate further to the plant tissues.These microbes can increase the levels of soil nutrients,and improve the physical and chemical properties of soil through nitrogenfixation,and phosphorus and potassium solubilization.In addition,soil microbes can promote the synthesis of hormones that increase plant growth and the accumulation of active compounds,eventually improving the quality of med-icinal plants.In 2022,the total value of the global microbial fertilizer market was$4.6 billion and is estimated to reach$10.36 billion by 2030.In this review,we have summarized the types of microbial fertilizers,the coloniza-tion and migration of microorganisms to plant tissues,and the beneficial effects of microbial fertilizers.In addi-tion,the prospects of developing microbial fertilizers and their application for medicinal plants have also been discussed.It aims to provide a reference for the rational application of microbial fertilizers in thefield of med-icinal plants and the green and sustainable development of medicinal plant resources.展开更多
Denitrification is one of the major processes causing nitrogen loss from arable soils.This study aimed to investigate the responses of nir S-type denitrifier communities to different chronic fertilization regimes acro...Denitrification is one of the major processes causing nitrogen loss from arable soils.This study aimed to investigate the responses of nir S-type denitrifier communities to different chronic fertilization regimes across the black soil region of Northeast China.Soil samples were collected from sites located in the north(NB),middle(MB),and south(SB)of the black soil region of Northeast China,each with four chronic fertilization regimes:no fertilizer(No F),chemical fertilizer(CF),manure(M),and chemical fertilizer plus manure(CFM).Methods of quantitative polymerase chain reaction(q PCR)and Illumina Mi Seq sequencing were applied to assess the abundance and composition of denitrifier communities by targeting the nir S gene.The results showed that the M and CFM regimes significantly increased the abundances of nir S-type denitrifiers compared with No F at the three locations.The majority of nir S sequences were grouped as unclassified denitrifiers,and the different fertilizers induced little variation in the relative abundance of known nir S-type denitrifier taxa.Over 90%of the sequences were shared among the four fertilization regimes at each location,but none of the abundant operational taxonomic units(OTUs)were shared among the three locations.Principal coordinate analysis(PCo A)revealed that the communities of nir S-type denitrifier were separated into three groups that corresponded with their locations.Although similar fertilization regimes did not induce consistent changes in the nir S-type denitrifier communities,soil p H and NO-3-N content simultaneously and significantly influenced the structure of nir S-type denitrifier communities at the three locations.Our results highlight that geographical separation rather than chronic fertilization was the dominant factor determining the nir S-type denitrifier community structures,and similar chronic fertilization regimes did not induce consistent shifts of nir S-type denitrifier communities in the black soils.展开更多
基金supported by Laboratory of Lingnan Modern Agriculture Project(NZ2021001)State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources(SKICUSA-a202007)Natural Science Foundation of Guangdong Province(2022A1515011027,2019A1515012009)。
文摘Plants are capable of regulating their shoot architecture in response to diverse internal and external environments.The circadian clock is an adaptive mechanism that integrates information from internal and ambient conditions to help plants cope with recurring environmental fluctuations.Despite the current understanding of plant circadian clock and genetic framework underlying plant shoot architecture,the intricate connection between these two adaptive mechanisms remains largely unclear.In this study,we elucidated how the core clock gene LUX ARRHYTHMO(LUX)regulates shoot architecture in the model legume plant Medicago truncatula.We show that mtlux mutant displays increased main stem height,reduced lateral shoot length,and decreased the number of lateral branches and biomass yield.Gene expression analysis revealed that Mt LUX regulated shoot architecture by repressing the expression of strigolactone receptor MtD14 and MtTB1/MtTCP1A,a TCP gene that functions centrally in modulating shoot architecture.In vivo and in vitro experiments showed that Mt LUX directly binds to a cis-element in the promoter of MtTB1/MtTCP1A,suggesting that Mt LUX regulates branching by rhythmically suppressing MtTB1/MtTCP1A.This work demonstrates the regulatory effect of the circadian clock on shoot architecture,offering a new understanding underlying the genetic basis towards the flexibility of plant shoot architecture.
基金supported by the National Natural Science Foundation of China Program(31871773 and 32001665)the Natural Science Foundation of Jiangsu Province(BK20200084)+3 种基金National First-Class Discipline Program of Food Science and Technology(JUFSTR20180102)Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Provincethe Wuxi Health and Family Planning Commission(ZDRC039)High-level Health Talents in Jiangsu Province(LGY2018016).
文摘This meta-analysis of randomized controlled trials aimed to evaluate the effects of probiotic supplementation on glucose homeostasis in patients with polycystic ovary syndrome(PCOS).The meta-analysis was performed in accordance with the Cochrane Handbook guidelines and relevant the preferred reporting items for systematic reviews and meta-analyses(PRISMA)statement criteria.Of 825 identified reports,11 randomized clinical trials were included in the meta-analysis.An analysis of pooled extracted data revealed that supplementation with probiotics significantly decreased fasting blood glucose(FBG,n=7;standardized mean difference(SMD)=−0.40;95%confidence interval(CI):−2.02,−0.02;P=0.04)and insulin levels(n=6;SMD=−0.57;95%CI:−0.89,−0.25;P=0.0004)and the homeostatic model assessment of insulin resistance(n=7;SMD=−0.64;95%CI:−0.96,−0.31;P=0.0001)while increasing the quantitative insulin sensitivity check index(QUICKI,n=5;SMD=0.58;95%CI:0.08,1.09;P=0.02)in patients with PCOS.The FBG-reducing effect decreased as the baseline body mass index(BMI)and mean age of the participants increased.Indeed,a greater number of bacterial species and a higher bacterial dose were shown to reduce QUICKI effectively.The systematic review indicated that probiotic supplementation may help to control glucose homeostasis in adults with polycystic ovarian syndrome.
基金supported by grants 82172507 (B.H.), 81700783 (B.H.)and 81672120 (D.J.) from the National Natural Science Foundation of China+1 种基金2019A1515011876 (B.H.) and 2018A030313937 (Z.L.) from the Guangdong Natural Science Fund Management Committee202002030176 (B.H.) from the Guangzhou Municipal Science and Technology Bureau
文摘Senescence impairs preosteoblast expansion and differentiation into functional osteoblasts,blunts their responses to bone formation-stimulating factors and stimulates their secretion of osteoclast-activating factors.Due to these adverse effects,preosteoblast senescence is a crucial target for the treatment of age-related bone loss;however,the underlying mechanism remains unclear.We found that mTORC1 accelerated preosteoblast senescence in vitro and in a mouse model.Mechanistically,mTORC1 induced a change in the membrane potential from polarization to depolarization,thus promoting cell senescence by increasing Ca^(2+)influx and activating downstream NFAT/ATF3/p53 signaling.We further identified the sodium channel Scn1a as a mediator of membrane depolarization in senescent preosteoblasts.Scn1a expression was found to be positively regulated by mTORC1 upstream of C/EBPα,whereas its permeability to Na^(+)was found to be gated by protein kinase A(PKA)-induced phosphorylation.Prosenescent stresses increased the permeability of Scn1a to Na^(+)by suppressing PKA activity and induced depolarization in preosteoblasts.Together,our findings identify a novel pathway involving mTORC1,Scn1a expression and gating,plasma membrane depolarization,increased Ca^(2+)influx and NFAT/ATF3/p53 signaling in the regulation of preosteoblast senescence.Pharmaceutical studies of the related pathways and agents might lead to novel potential treatments for agerelated bone loss.
文摘JF305 is a highly prolific pancreatic cancer cell line that originated from a Chinese patient. The cell line bears a functional HR double strand DNA repair mechanism but very responsive to PARP treatment a phenomenon clearly suggesting presence of an anomaly in the mechanism. Brca1, Brca2 and CHK2 proteins are very important constituents of the HR mechanism whose respective gene coding mutations are strongly associated with several cancers and are widely exploited in anticancer chemotherapy. In this current study, the BRCA1, BRCA2 gene mutation status in JF305 was determined together with the presence of 3 widely reported cancer linked CHK2 founder mutations (1100delC, I157T, IVS2 +IG > A). CHK21100delC genotype was determined using allele specific PCR, while the PCR-RFLP assay was used for I157T, IVS2 +IG > A analysis. PCR and direct sequencing were used for assessing the BRCA1 and BRCA2 gene. Results revealed that JF305 is CHK21100delC heterozygous mutant, CHK2I157T and CHK2IVS2 +IG > A wild type. Furthermore, it was observed that JF305 lacked BRCA1 and BRCA2 gene mutations. The mutation status identification of CHK2 and BRCA1/2 in JF305 provides a major milestone towards elucidating the properties of the cell line which subsequently promises to be an excellent model for evaluating the role of parp inhibitors in pancreatic cancer chemotherapy most especially in the respective cancer cell lines without BRCA1 and BRCA2 gene mutations.
文摘Background: Gemcitabine is a deoxycytidine analog, which is used as first-line agent for pancreatic cancer therapy, and its efficacy relied on its intracellular conversion to active triphosphate form. However, administration with gemcitabine still has limited effect on the overall survival of patients with pancreatic cancer. Objective: We aimed to study the combination effect of gemcitabine and doxorubicin to pancreatic cancer cells BxPC3 and PANC1, and unveil the mechanism. Methods: The study was performed in pancreatic cancer cells PANC1 and BxPC3, the contribution of UMP/CMP kinase 1 (CMPK1) to gemcitabine in PANC1 and BxPC3 cells was measured by transfection of CMPK1 plasmid or CMPK1 siRNA treatment to adjust the expression of CMPK1 in the cells;then analyzed the cell vitality and migration after treated with 1% IC50 of doxorubicin and gemcitabine or only with gemcitabine;the activity of CMPK1 and the effect of doxorubicin to the reaction was measured by HPLC assay in vitro;at last, docking analysis by computer was used to calculate the possible interaction sites of CMPK1 to DOX. Results: The sensitivity of PANC1 and BxPC3 cells to gemcitabine was improved when increasing the expression of CMPK1, and decreased when knockout CMPK1 by CMPK1 siRNA in BxPC3 cells;when combined with doxorubicin, the sensitivity of PANC1 and BxPC3 cells to gemcitabine also increased, and the cells migration reduced;we further found out that by adding 10 μM doxorubicin, the catalyzing activity of CMPK1 elevated about 2 times in vitro;the docking result showed that the association of CMPK1 to DOX was mainly by hydrogen bond and ionic interaction. Conclusion: CMPK1 can catalyze gemcitabine to its active form within the cells so that the sensitivity of the cells to gemcitabine elevated, and doxorubicin may enhance the cytotoxic effect to pancreatic cancer by up-regulate the activity of CMPK1, the combination of these deoxycytidine analogs with DOX might exert better efficacy.
文摘Membrane technology has widely accepted as the most energy efficient technology for the separation of ions or molecules. As the core of the membrane technology, the chemical and structure nature of membrane materials determines the application and energy-efficiency of the technology. An advanced membrane that has a remarkable ability to selectively differentiate ions and molecules can significantly enhance the separation technology and revolutionize industrial separation processes.
文摘CO 2 is a main component of gases in the Yangbajing geothermal field, usually higher than 85 in volume; others are N 2, H 2S, H 2, CH 4, etc. Helium R/R a ratios, ranging from 0.14 to 0.46, indicate a crustal component to be dominant in the gases. δ 13C-CO 2 values and δ 34S-H 2S values are in the range -7.72‰--11.33‰ and 0.2‰-8.3‰, respectively. The gases are inferred to mostly derive from the partial melting of the Nyainqentanglha core complex. The gases of shallow and deep reservoirs are distinctive in chemical compositions and δ 34S-H 2S values, which shows their different flowing paths and physical-chemical processes. Sulphur does not approach isotopic equilibrium between H 2S and SO 2- 4 species in both reservoirs. Significance of steam chemical monitoring is also discussed.
基金the National Natural Science Foundation of China(32060174 and 32160079)the Natural Science Foundation of Guangxi Province(2020GXNSFAA297236 and 2020GXNSFAA297211)+2 种基金Fellowship of the China Postdoctoral Science Foundation(2021M693175)the State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources(SKLCUSAa202007)the Innovation Project of Guangxi Graduate Education(YCBZ2023037).
文摘Plant architecture and panicle architecture are two critical agronomic traits that greatly affect the yield of rice(Oryza sativa).PROSTRATE GROWTH 1(PROG1)encodes a key C2H2-type zinc-finger transcription factor and has pleiotropic effects on the regulation of both plant and panicle architecture,thereby influencing the grain yield.However,the molecular mechanisms through which PROG1 controls plant and panicle architecture remain unclear.In this study,we showed that PROG1 directly binds the LAZY 1(LA1)promoter and acts as a repressor to inhibit LA1 expression.Conversely,LA1 acts as a repressor of PROG1 by directly binding to the PROG1 promoter.These two genes play antagonistic roles in shaping plant architecture by regulating both tiller angle and tiller number.Interestingly,our data showed that PROG1 controls panicle architecture through direct binding to the intragenic regulatory regions of OsGIGANTEA(OsGI)and subsequent activation of its expression.Collectively,we have identified two crucial targets of PROG1,LA1 and OsGI,shedding light on the molecular mechanisms underlying plant and panicle architecture control by PROG1.Our study provides valuable insights into the regulation of key domestication-related traits in rice and identifies potential targets for future high-yield rice breeding.
基金supported by the National Natural Science Foundation of China(U22A20328 and 22074043)Science and Technology Commission of Shanghai Municipality(20430711800 and23ZR1475000)Lingang Laboratory(LG-QS-202206-04)。
文摘Diseases caused by invasive pathogens are an increasing threat to forest health,and early and accurate disease detection is essential for timely and precision forest management.The recent technological advancements in spectral imaging and artificial intelligence have opened up new possibilities for plant disease detection in both crops and trees.In this study,Dutch elm disease(DED;caused by Ophiostoma novo-ulmi,)and American elm(Ulmus americana)was used as example pathosystem to evaluate the accuracy of two in-house developed high-precision portable hyper-and multi-spectral leaf imagers combined with machine learning as new tools for forest disease detection.Hyper-and multi-spectral images were collected from leaves of American elm geno-types with varied disease susceptibilities after mock-inoculation and inoculation with O.novo-ulmi under green-house conditions.Both traditional machine learning and state-of-art deep learning models were built upon derived spectra and directly upon spectral image cubes.Deep learning models that incorporate both spectral and spatial features of high-resolution spectral leaf images have better performance than traditional machine learning models built upon spectral features alone in detecting DED.Edges and symptomatic spots on the leaves were highlighted in the deep learning model as important spatial features to distinguish leaves from inoculated and mock-inoculated trees.In addition,spectral and spatial feature patterns identified in the machine learning-based models were found relative to the DED susceptibility of elm genotypes.Though further studies are needed to assess applications in other pathosystems,hyper-and multi-spectral leaf imagers combined with machine learning show potential as new tools for disease phenotyping in trees.
基金supported by the National Key Research and Development Program of China(2021ZD0113601)Key Research and Development Projects of Huzhou City(2021ZD2037).
文摘Currently,the presence of genetically modified(GM)organisms in agro-food markets is strictly regulated by enacted legislation worldwide.It is essential to ensure the traceability of these transgenic products for food safety,consumer choice,environmental monitoring,market integrity,and scientific research.However,detecting the existence of GM organisms involves a combination of complex,time-consuming,and labor-intensive techniques requiring high-level professional skills.In this paper,a concise and rapid pipeline method to identify transgenic rice seeds was proposed on the basis of spectral imaging technologies and the deep learning approach.The composition of metabolome across 3 rice seed lines containing the cry1Ab/cry1Ac gene was compared and studied,substantiating the intrinsic variability induced by these GM traits.Results showed that near-infrared and terahertz spectra from different genotypes could reveal the regularity of GM metabolic variation.The established cascade deep learning model divided GM discrimination into 2 phases including variety classification and GM status identification.It could be found that terahertz absorption spectra contained more valuable features and achieved the highest accuracy of 97.04%for variety classification and 99.71%for GM status identification.Moreover,a modified guided backpropagation algorithm was proposed to select the task-specific characteristic wavelengths for further reducing the redundancy of the original spectra.The experimental validation of the cascade discriminant method in conjunction with spectroscopy confirmed its viability,simplicity,and effectiveness as a valuable tool for the detection of GM rice seeds.This approach also demonstrated its great potential in distilling crucial features for expedited transgenic risk assessment.
基金funded by the Science and Technology Innovation Program of Hunan Province(2022RC1224)the Training Program for Excellent Young Innovators of Changsha(kq2305028).
文摘Medicinal plants have aroused considerable interest as an alternative to chemical drugs due to the beneficial effects of their active secondary metabolites.However,the extensive use of chemical fertilizers and pesticides in pursuit of yield has caused serious pollution to the environment,which is not conducive to sustainable devel-opment in thefield of medicinal plants.Microbial fertilizers are a type of“green fertilizer”containing specific microorganisms that can improve the soil microbial structure,enhance plant resistance to biological and abiotic stresses,and increase the yield of medicinal plants.The root exudates of medicinal plants attract different micro-organisms to the rhizosphere,which then migrate further to the plant tissues.These microbes can increase the levels of soil nutrients,and improve the physical and chemical properties of soil through nitrogenfixation,and phosphorus and potassium solubilization.In addition,soil microbes can promote the synthesis of hormones that increase plant growth and the accumulation of active compounds,eventually improving the quality of med-icinal plants.In 2022,the total value of the global microbial fertilizer market was$4.6 billion and is estimated to reach$10.36 billion by 2030.In this review,we have summarized the types of microbial fertilizers,the coloniza-tion and migration of microorganisms to plant tissues,and the beneficial effects of microbial fertilizers.In addi-tion,the prospects of developing microbial fertilizers and their application for medicinal plants have also been discussed.It aims to provide a reference for the rational application of microbial fertilizers in thefield of med-icinal plants and the green and sustainable development of medicinal plant resources.
基金the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB15010103)the National Key Research and Development Program of China(No.2017YFD0200604)+1 种基金the National Natural Science Foundation of China(No.41771284)the Chinese Biodiversity Monitoring and Research Network(Sino BON).
文摘Denitrification is one of the major processes causing nitrogen loss from arable soils.This study aimed to investigate the responses of nir S-type denitrifier communities to different chronic fertilization regimes across the black soil region of Northeast China.Soil samples were collected from sites located in the north(NB),middle(MB),and south(SB)of the black soil region of Northeast China,each with four chronic fertilization regimes:no fertilizer(No F),chemical fertilizer(CF),manure(M),and chemical fertilizer plus manure(CFM).Methods of quantitative polymerase chain reaction(q PCR)and Illumina Mi Seq sequencing were applied to assess the abundance and composition of denitrifier communities by targeting the nir S gene.The results showed that the M and CFM regimes significantly increased the abundances of nir S-type denitrifiers compared with No F at the three locations.The majority of nir S sequences were grouped as unclassified denitrifiers,and the different fertilizers induced little variation in the relative abundance of known nir S-type denitrifier taxa.Over 90%of the sequences were shared among the four fertilization regimes at each location,but none of the abundant operational taxonomic units(OTUs)were shared among the three locations.Principal coordinate analysis(PCo A)revealed that the communities of nir S-type denitrifier were separated into three groups that corresponded with their locations.Although similar fertilization regimes did not induce consistent changes in the nir S-type denitrifier communities,soil p H and NO-3-N content simultaneously and significantly influenced the structure of nir S-type denitrifier communities at the three locations.Our results highlight that geographical separation rather than chronic fertilization was the dominant factor determining the nir S-type denitrifier community structures,and similar chronic fertilization regimes did not induce consistent shifts of nir S-type denitrifier communities in the black soils.