鲟鱼工厂化流水养殖尾水排放及处理模式的探究与示范

随着水产养殖业的快速发展,传统水产养殖与水资源的矛盾日益突出,而鲟鱼工厂化流水养殖用水量大、养殖尾水排放多等原因,不可避免地带来养殖水体环境污染和富营养化问题,如果不及时有效进行处理,将会给生态环境带来较大影响。该研究基于水产养殖业绿色发展理念和淡水池塘养殖水排放要求,对山东省泗水县鲟鱼工厂化流水养殖尾水处理现有模式及水质特征进行了总结分析,并提出了下一步工作措施,旨在为下一步开展鲟鱼工厂化流水养殖尾水治理指导工作提供参考和借鉴。

Textural and compositional zoning in plagioclase phenocrysts:implications for magma chamber processes in the Emeishan large Igneous Province,SW China

Textural and compositional zoning within plagioclase phenocrysts records the magma chamber processes,such as magma differentiation,magma recharge and mixing,and crustal contamination.The plagioclase phenocrysts in the Daqiao and Qiaojia plagioclase-phyric basalts from the Emeishan Large Igneous Province(LIP)show complex textural and compositional zoning patterns,e.g.,normal,reverse,oscillatory,and patchy zoning patterns.Most plagioclase phenocrysts exhibit a core–rim normal zoning pattern(Pl-A)with euhedral high-An cores(An=76–78%,in mole fraction)and low-An rims(An=68–72%),indicative of the crystal regrowth processes caused by recharge of relatively evolved magmas after the formation of high-An cores.Some phenocrysts have a core–rim reverse zoning pattern(Pl-B)with irregular ovaloid cores,characterized by extremely low An(60–61 mol%)and Ba(84–88 ppm)contents and extremely high87Sr/86Sr ratios(0.7120–0.7130).The rims of the Pl-B have relatively high An(69–72%),Ba(~160 ppm)contents,and low87Sr/86Sri(~0.7056).These Pl-B plagioclase phenocrysts preserve the information about the interaction between the crustal xenocrysts and the transporting magmas.Some plagioclase phenocrysts show a core–mantle–rim oscillatory zoning pattern(Pl-C)with multiple oscillations of An(70–80%),Ba(88–147ppm)from core to rim,revealing replenishment and mixing of multiple batches of basaltic melts with diverse compositions.87Sr/86Sr ratios of the Pl-C do not vary significantly(0.7050–0.7054).A small portion of phenocrysts has patchy patterns in the cores(Pl-D),where the low-An patches(72–75%)in form of elliptical or irregular elongated shapes were enclosed by the high-An domains(80–87%).These features can be attributed to crystal dissolution and regrowth processes during the reaction between earlyformed low-Cumulates and recharged hot primitive melts.The cores,mantles,and rims of different types of plagioclase phenocrysts(except the core of Pl-B)commonly display nearly constant Sr isotopic compositions,implying insignificant wall-rock assimilation at shallow-level magma reservoir(s)during the growth of these plagioclase phenocrysts.In conclusion,the massive crystallization of plagioclase in the late stage was an important controlling factor for the formation of iron-rich basalts in the Emeishan LIP.

3D Human Pose Estimation Using Two-Stream Architecture with Joint Training

With the advancement of image sensing technology, estimating 3Dhuman pose frommonocular video has becomea hot research topic in computer vision. 3D human pose estimation is an essential prerequisite for subsequentaction analysis and understanding. It empowers a wide spectrum of potential applications in various areas, suchas intelligent transportation, human-computer interaction, and medical rehabilitation. Currently, some methodsfor 3D human pose estimation in monocular video employ temporal convolutional network (TCN) to extractinter-frame feature relationships, but the majority of them suffer from insufficient inter-frame feature relationshipextractions. In this paper, we decompose the 3D joint location regression into the bone direction and length, wepropose the TCG, a temporal convolutional network incorporating Gaussian error linear units (GELU), to solvebone direction. It enablesmore inter-frame features to be captured andmakes the utmost of the feature relationshipsbetween data. Furthermore, we adopt kinematic structural information to solve bone length enhancing the use ofintra-frame joint features. Finally, we design a loss function for joint training of the bone direction estimationnetwork with the bone length estimation network. The proposed method has extensively experimented on thepublic benchmark dataset Human3.6M. Both quantitative and qualitative experimental results showed that theproposed method can achieve more accurate 3D human pose estimations.

内镜下黏膜切除术与内镜黏膜下剥离术治疗结直肠癌临床疗效的Meta分析

目的系统性评价内镜下黏膜切除术(EMR)与内镜黏膜下剥离术(ESD)治疗结直肠癌的疗效。方法计算机检索PubMed、The Cochrane Library、EMbase、CNKI、CBM和WanFang数据库,查找所有比较EMR与ESD治疗结直肠癌的随机对照试验(RCT),检索时限为从建库至2018年12月16日。同时手动检索纳入研究的参考文献,按照纳入和排除标准筛选文献、提取资料,评价纳入研究的方法质量学,数据采用RevMan 5.3软件进行Meta分析。结果最终纳入18个RCT,共1764例患者。Meta分析结果显示:①有效性方面:ESD整块切除率高(OR^=0.16,95%CI:0.09~0.27,P=0.000),组织治愈性切除率高(OR^=0.32,95%CI:0.11~0.90,P=0.030),复发率低(OR^=6.15,95%CI:3.42~11.06,P=0.000);②安全性方面:ESD手术时间长(MD=-25.53,95%CI:-33.37^-17.69,P=0.000),术中穿孔率较高(OR^=0.22,95%CI:0.10~0.47,P=0.000),术中出血率和感染率差异均无统计学意义(OR^=0.67,95%CI:0.43~1.05,P=0.080;OR^=0.57,95%CI:0.22~1.49,P=0.250)。结论现有证据表明,ESD较EMR对结直肠癌病变患者的治疗有效性高且复发率低,但手术时间较长、穿孔率高。受纳入研究质量和数目所限,以上结论尚需进一步开展更多大样本、高质量及多中心的RCT加以定论。

干摩擦下含不同角度预制裂纹丁腈橡胶的裂纹扩展

干摩擦下橡胶裂纹的扩展方向与初始预制裂纹角度息息相关。目前人们对于橡胶裂纹扩展研究主要集中在橡胶材料配方对裂纹扩展的影响以及在拉压或剪切作用下裂纹的扩展规律等方面,很少有人研究干摩擦下初始预制裂纹角度对裂纹扩展的影响。文中通过往复式摩擦试验和ANSYS有限元分析,观察不同预制裂纹角度橡胶试样的裂纹扩展过程,并计算裂纹尖端撕裂能大小。结果表明,裂纹扩展过程中扩展路径基本上可分为初始扩展阶段、水平扩展阶段和最后断裂阶段;随着裂纹角度的增加,裂纹扩展三个阶段所需的时间都有所延长;裂纹尖端的撕裂能随初始预制裂纹角度的增加而逐渐增大,有限元分析结果很好地证实了试验结果。

羧甲司坦对人气道上皮细胞炎症的影响及调控机制研究

目的通过比较羧甲司坦单用或联合布地奈德对人气道上皮细胞炎症的影响探讨其调控机制。方法MTT法检测不同浓度烟雾提取物(CSE)对细胞生存率的影响。给予不同药物抑制剂和激动剂干预后,检测炎症因子水平、ERK和IκBα蛋白,以及ERK和NF-κB mRNA的表达。结果模型组炎症因子水平、p-ERK和p-IκBα蛋白,以及ERK和NF-κB mRNA表达均增高;用药组上述指标较模型组均有所改善,但用药组之间比较差异无统计学意义(P>0.05)。羧甲司坦、PD98059干预后p-ERK蛋白及ERK、NF-κB mRNA水平均降低,而给予EGF干预后上述指标较羧甲司坦组有所回升。结论羧甲司坦的抗炎机制可能与糖皮质激素存在重叠,基础应用糖皮质激素者由于抑制了相同的信号转导通路,故再联合应用羧甲司坦时效果不佳。

缺陷晶体硅太阳电池热斑实验

设计了室内外缺陷电池热斑情况的测试方案,将缺陷电池反接12 V直流电压,使电池片处于反向偏置状态,并对比了电池片的温度分布和漏电流大小,测试了不同反偏电压下温度分布和电流变化。提出了一种缺陷电池测试方法,将单块电池接入组件,在不同遮挡比例下测试组件的I-V、P-V曲线,对比不同类型缺陷电池的差异。

高效液相色谱法测定渔业用水中喹乙醇代谢物残留量

建立了固相萃取-高效液相色谱测定渔业用水中喹乙醇代谢物残留量的方法。3-甲基喹噁啉-2羧酸(MQCA)经PAX固相萃取柱纯化和富集,用含2%甲酸的乙酸乙酯溶液洗脱,甲醇和1. 0%甲酸水溶液作为流动相,经C18柱分离,10 min内出峰。MQCA在0. 01～1. 00 mg/m L范围内线性良好,相关系数为0. 999 91,外标法定量,MQCA的检出限为0. 01 mg/L,定量限为0. 02 mg/L,平均加标回收率为79. 0%～99. 3%,相对标准偏差(RSD)为7. 38%～9. 12%。利用该方法对山东省济宁市采煤塌陷区重点养殖区的渔业用水进行分析,8个站位中MQCA组分的含量均低于检出限。本方法适用于检测渔业用水中MQCA的残留,可为渔业环境保护提供技术支持。

Evaluation of gastric lavage efficiency and utility using a rapid quantitative method in a swine paraquat poisoning model

BACKGROUND:Gastric lavage(GL)is one of the most critical early therapies for acute paraquat(PQ)poisoning;however,details of the treatment protocol remain to be established.METHODS:A rapid quantitative method involving sodium dithionite testing was developed.It was validated for the determination of the PQ concentrations in gastric juice and eluate samples from a swine acute PQ poisoning model with early or delay GL,or without.The vital signs,laboratory testing,and PQ plasma concentrations were collected for therapeutic effect evaluation.RESULTS:The reaction conditions of the test were optimized for two types of samples.Early GL at one hour(H1)could improve the signs and symptoms after acute PQ poisoning at 24 hours(H24).In contrast,GL at 6 hours(H6)could only partially relieve the vital signs.The H1 GL group effectively reduced the peak of the plasma PQ concentration.In addition,the PQ concentrations in the plasma and the gastric juice were significantly decreased in both the GL groups as compared to the untreated group at H24.Moreover,there was no significant difference in the washing efficiencies calculated from the total eluates between the two GL groups.However,the washing efficiency of the first 10 L eluate is superior to that of the additional 10 L eluate.CONCLUSION:GL only at early stage may it benefit PQ poisoning in an animal model.The currently used 20 L GL volume may need to be reduced in view of the low washing efficiency in the later 10 L eluate.The rapid quantitative method can be used for gastric juice sample and has a certain value for clinical GL practices.

EVALUATION OF INTERNATIONAL CLASSIFICATION CRITERIA (2002) FOR PRIMARY SJGREN'S SYNDROME IN CHINESE PATIENTS

Objective To evaluate the sensitivity and specificity of international classification criteria (2002) for primary Sjgren’s syndrome (pSS) and the role of lower lip biopsy in diagnosis of pSS in Chinese patients. Methods Patients who were diagnosed by the experts/rheumatologists as pSS during 1990-2002 from the Depart- ment of Rheumatology, Peking Union Medical College Hospital were retrospectively collected as experimental group. Patients who were diagnosed as non-pSS connective tissue diseases or non-connective tissue diseases served as control group. Those with a history of head-neck radiation, hepatitis C virus infection, AIDS, lymphoma, sarcoidosis, graft versus host disease (GVHD), and anti-acetylcholine drug use were exempted. Both groups were required to complete question- naires about symptoms such as dry eyes and dry mouth, and complete the objective tests of keratoconjunctivitis and xero- stomia including Schirmer test, corneal staining, unstimulated salivary flow, sialography, lower lip biopsy, and antinuclear antibodies (including anti-SSA/SSB antibodies) test. Results A total of 330 pSS patients were included in experimental group and 185 non-pSS patients in control group. The mean age of both groups matched (47.8 ± 10.9 years vs. 46.2 ± 13.6 years, P > 0.05). The sensitivities of the criteria in pSS patients with lower lip biopsy and in pSS patients without lower lip biopsy were 89.2% and 87.2%, respectively; the overall sensitivity was 88.5%. The specificity was 97.3%. A total of 11.3% pSS patients with negative anti-SSA/SSB anti- bodies were diagnosed as pSS by lower lip biopsy. Conclusion The international classification criteria (2002) for pSS is feasible in Chinese patients. It has high sensitivity and specificity, and may serve as diagnosis criteria in routine clinical practice.

Inhibitive effect of triptolide on invasiveness of human fibrosarcoma cells by downregulating matrix metalloproteinase-9 expression

Objective:To explore the molecular mechanisms of antitumor properties of triptolide,a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook F.Methods:Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h.Then the expression and activity of matrix metalloproteinase(MMP)-2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9- treated HT-1080 cells.Results:18 nmol/L triptolide inhibited the gene expression and activity of MMP-9,but not those of MMP-2,in HT-1080 cells.In addition,both 18 nmol/L triptolide and 3μg/mL anti-MMP-9 significantly reduced the invasive potential of HT-1080 cells,by about 50%and 35%, respectively,compared with the control.Whereas there was no significant difference between the effect of 18 nmol/L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells. Conclusions:These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.

Softening–melting behavior of mixed burden based on low-magnesium sinter and fluxed pellets

A low MgO content in sinter is conducive to reduce the MgO content in blast furnace slag.This study investigated the effect of MgO content in sinter on the softening–melting behavior of the mixed burden based on fluxed pellets.When the MgO content increased from 1.31 wt% to 1.55 wt%, the melting temperature of sinter increased to 1521℃.Such an increase was due to the formation of the high-meltingpoint slag phase.The reduction degradation index of sinter with 1.31 wt% MgO content was better than that of others.The initial softening temperature of the mixed burden increased from 1104 to 1126℃ as MgO content in sinter increased from 1.31 wt% to 1.55 wt%, and the melting temperature decreased from 1494 to 1460℃.The permeability index(S-value) of mixed burden decreased to 594.46 kPa·℃ under a high MgO content with 1.55 wt%, indicating that the permeability was improved.The slag phase composition of burden was mainly akermarite(Ca_(2)MgSiO_(7)) when the MgO content in sinter was 1.55 wt%.The melting point of akermarite is 1450℃, which is lower than other phases.

Effect of β-sodium aescinate on hypoxia-inducible factor-1α expression in rat brain cortex after cardiopulmonary resuscitation

BACKGROUND:This study was undertaken to investigate the expression of hypoxia-inducible factor-1a(HIF-1a) in rat cerebral cortex and the effects of p-sodium aescinate(SA) administration after return of spontaneous circulation(ROSC).METHODS:Sixty rats were divided into three groups:SA group,injected intraperitoneally with SA instantly after ROSC;control group,injected intraperitoneally with normal saline;and shamoperated group,without cardiac arrest or SA.The cardiac arrest model was established using asphyxiation and intravenous potassium chloride.Blood was sampled 1,6,12,and 24 hours after ROSC.Protein and mRNA levels of HIF-1a,VEGF and EPO were detected in the cerebral cortex by immunohistochemistry and real-time RT-PCR;serum levels of NSE and S100 P were determined by enzyme-linked immunosorbent assays.RESULTS:Serum S100β and NSE were significantly increased in the control group versus the sham-operated group 1,6,12 and 24 hours after ROSC(P<0.05).Protein and mRNA levels of HIF-1a,VEGF and EPO were significantly increased in the control rats(P<0.05).Serum NSE and S100 P were significantly decreased in the SA group versus the control group 1,6,12 and 24 hours after ROSC(P<0.05).Protein and mRNA levels of HIF-1a,VEGF and EPO were significantly increased in the SA group(P<0.05).CONCLUSIONS:The expression of HIF-1a is increased in rat cerebral cortex after ROSC,and SA up-regulates the expression of HIF-1α.The up-regulation of HIF-1α improves the resistance of the cortex to ischemia and hypoxia and contributes to neuroprotection,possibly because of up-regulation of EPO and VEGF expression.

Schistosoma japonicum attenuates dextran sodium sulfateinduced colitis in mice via reduction of endoplasmic reticulum stress

AIM To elucidate the impact of Schistosoma(S.) japonicum infection on inflammatory bowel disease by studying the effects of exposure to S. japonicum cercariae on dextran sodium sulfate(DSS)-induced colitis. METHODS Infection was percutaneously established with 20 ± 2 cercariae of S. japonicum, and colitis was induced by administration of 3% DSS at 4 wk post infection. Weight change, colon length, histological score(HS) and disease activity index(DAI) were evaluated. Inflammatory cytokines, such as IL-2, IL-10 and IFN-γ, were tested by a cytometric bead array and real-time quantitative polymerase chain reaction(RT-PCR). Protein and m RNA levels of IRE1α, IRE1β, GRP78, CHOP, P65, P-P65, P-IκBα and IκBα in colon tissues were examined by Western blot and RT-PCR, respectively. Terminal deoxynucleotidyl transferase-mediated d UTP nick-end labeling positive cells, cleaved-caspase 3 expression and Bcl2/Bax were investigated to assess the apoptosis in colon tissues.RESULTS Mice infected with S. japonicum cercariae were less susceptible to DSS. Mice infected with S. japonicum cercariae and treated with DSS showed decreased weight loss, longer colon, and lower HS and DAI compared with mice treated with DSS alone. A substantial decrease in Th1/Th2/Th17 response was observed after infection with S. japonicum. Endoplasmic reticulum(ER) stress and the nuclear factor-kappa B(NF-κB) pathway were reduced in mice infected with S. japonicum cercariae and treated with DSS, along with ameliorated celluar apoptosis, in contrast to mice treated with DSS alone. CONCLUSION Exposure to S. japonicum attenuated inflammatory response in a DSS-induced colitis model. In addition to the Th1/Th2/Th17 pathway and NF-κB pathway, ER stress was shown to be involved in mitigating inflammation and decreasing apoptosis. Thus, ER stress is a new aspect in elucidating the relationship between helminth infection and inflammatory bowel disease(IBD), which may offer new therapeutic methods for IBD.

Antimicrobial peptides: mechanism of action, activity and clinical potential

The management of bacterial infections is becoming a major clinical challenge due to the rapid evolution of antibiotic resistant bacteria.As an excellent candidate to overcome antibiotic resistance,antimicrobial peptides(AMPs)that are produced from the synthetic and natural sources demonstrate a broad-spectrum antimicrobial activity with the high specificity and low toxicity.These peptides possess distinctive structures and functions by employing sophisticated mechanisms of action.This comprehensive review provides a broad overview of AMPs from the origin,structural characteristics,mechanisms of action,biological activities to clinical applications.We finally discuss the strategies to optimize and develop AMP-based treatment as the potential antimicrobial and anticancer therapeutics.

Parameter estimation for rigid body after micro-Doppler removal based on L-statistics in the radar analysis

In traditional inverse synthetic aperture radar （ISAR） imaging of moving targets with rotational parts, the micro-Doppler （m-D） effects caused by the rotational parts influence the quality of the radar images. Recently, L. Stankovic proposed an m-D removal method based on L-statistics, which has been proved effective and simple. The algorithm can extract the m-D effects according to different behaviors of signals induced by rotational parts and rigid bodies in time-frequency （T-F） domain. However, by removing m-D effects, some useful short time Fourier transform （STFT） samples of rigid bodies are also extracted, which induces the side lobe problem of rigid bodies. A parameter estimation method for rigid bodies after m-D removal is proposed, which can accurately re- cover rigid bodies and avoid the side lobe problem by only using m-D removal. Simulations are given to validate the effectiveness of the proposed method.

Research on the Weight of Index for the Assessment Standard for Green Store Building

Assessment standard for green store building of national standard has been compiled. The index has been given by experts' subjective assignment based on experience. Analytic hierarchy process has been used,making questionnaires of experts,using pairwise comparison and calculating the weight of each index. The weight of index in assessment would not only make quantitative evaluation on the whole situation of green store buildings,but also provide the foundation for compilation and revision.

Operating Experience of Country’s Agricultural Science and Technological Park

Through the analysis of the operation of China’s agricultural science technological and development process, the paper summed up the basic experience of agricultural science technological park and operation mode, in order to run at all levels of agricultural science technological park of science and regional some beneficial references for the development of agricultural economy.

Differences in expression of retinal proteins between diabetic and normal rats

瞄准：为了在在正常、糖尿病的老鼠之间的网膜的蛋白质的表示比较并且识别差别，并且分析网膜的疾病的分子的病原的机制，由糖尿病引起了。方法：在从糖尿病、正常的老鼠的网膜的纸巾的蛋白质表示的变化用 2-dimensional polyacrylamide 胶化电气泳动(2-DE ) 被观察。展出统计上重要的变化的一些蛋白质点(P 【 0.05 ) 随机被选择并且由双人脚踏车团分光术识别了并且由生物信息学分析了。结果：2-DE 证明表示在 5 网膜的蛋白质是起来调整的，在 23 网膜的蛋白质下面调整，并且消失在 8 网膜的蛋白质。八个点被双人脚踏车团分光术(MS/MS ) 从 36 个点识别并且由生物信息学分析了。Guanylate 激酶 1， triosephosphate 异构酶 1， 2 玩了的 ATP synthase 子单元 d，白朊和 dimethylarginine dimethylaminohydrolase 在信号转导变异的一个重要角色。Triosephosphate 异构酶 1，晶状体蛋白高山哈 B， ATP synthase 子单元 d 和 peroxiredoxin 6 涉及网膜的纸巾的能代谢。1 玩了的 Guanylate 激酶在网膜的杆光敏电阻器房间的相片刺激的一个重要角色。晶状体蛋白贝它 A1 是否在糖尿病的视网膜起一个作用，到目前为止是未知的。结论：在在糖尿病、正常的老鼠之间的网膜的蛋白质的表示有差别。这些蛋白质可以涉及糖尿病引起的网膜的疾病的机制和预后。

Development and evaluation of a predictive nomogram for survival in heat stroke patients: a retrospective cohort study

BACKGROUND:This study aimed to establish an eff ective nomogram to predict the survival of heat stroke(HS)based on risk factors.METHODS:This was a retrospective,observational multicenter cohort study.We analyzed patients diagnosed with HS,who were treated between May 1 and September 30,2018 at 15 tertiary hospitals from 11 cities in Northern China.RESULTS:Among the 175 patients,32 patients(18.29%)died before hospital discharge.After the univariate analysis,mechanical ventilation,initial mean arterial pressure<70 mmHg,maximum heart rate,lab results on day 1(white blood cell count,alanine aminotransferase,creatinine),and Glasgow admission prediction score were included in multivariate analysis.Multivariate Cox regression showed that invasive ventilation,initial mean arterial pressure<70 mmHg(1 mmHg=0.133 kPa),and Glasgow admission prediction score were independent risk factors for HS.The nomogram was established for predicting 7-d and 14-d survival in the training cohort.The nomogram exhibited a concordance index(C-index)of 0.880(95%confidence interval[95%CI]0.831-0.930)by bootstrapping validation(B=1,000).Furthermore,the nomogram performed better when predicting 14-d survival,compared to 7-d survival.The prognostic index cut-off value was set at 2.085,according to the operating characteristic curve for overall survival prediction.The model showed good calibration ability in the internal and external validation datasets.CONCLUSION:A novel nomogram,integrated with prognostic factors,was proposed;it was highly predictive of the survival in HS patients.