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真空紫外激光解吸/电离单细胞质谱成像装置的研制和调试
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作者 邢蕾 牟含章 +3 位作者 潘建斌 康斌 徐静娟 陈洪渊 《应用化学》 CAS CSCD 北大核心 2024年第1期100-108,共9页
开发同时具有亚微米分辨率以及高检测灵敏度的无基质质谱成像方法,对于可视化单细胞生物样品具有重要意义。在此,提出了真空紫外激光解吸/电离飞行时间质谱成像(VUVDI-ToFMSI)方法。通过调整基频光波长至777.7、403.22和255 nm,汞蒸气... 开发同时具有亚微米分辨率以及高检测灵敏度的无基质质谱成像方法,对于可视化单细胞生物样品具有重要意义。在此,提出了真空紫外激光解吸/电离飞行时间质谱成像(VUVDI-ToFMSI)方法。通过调整基频光波长至777.7、403.22和255 nm,汞蒸气池中氩气气压至1100 Pa以及温度至240℃,获得接近13μJ/pulse的高强度VUV激光能量;同时,设计的同轴光路聚焦VUV激光至亚微米水平;另外,通过进一步调整偏转电场、离子聚焦电场以及反射电场的电负性以及电压等参数,离子的飞行路径得到矫正与优化。基于这种方法,实现了单个HeLa细胞中外源性药物亚甲基蓝(m/z 284.4)、内源性代谢物离子信号m/z 152.1以及m/z 81.1的纳米级成像(~500 nm/pixel),结果表明它们分别富集在细胞质、细胞核以及核仁内,证明了该方法在绘制单细胞不同区室内外源和内源性化学物质的高分辨以及高灵敏度的成像能力。该VUVDI-ToFMSI平台将为了解更多生物样本的精细结构与功能提供更完整、更深入的化学信息。 展开更多
关键词 单细胞 质谱成像 真空紫外 亚微米分辨
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双极纳米电极阵列实现单个铂纳米颗粒上氢气析出反应的电致化学发光成像 被引量:1
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作者 秦祥 李仲秋 +3 位作者 潘建斌 李剑 王康 夏兴华 《电化学》 CAS CSCD 北大核心 2021年第2期157-167,共11页
本文制备了嵌于多孔阳极氧化铝(AAO)膜中直径为200 nm,间距为450 nm的高密度(5.7×10^(8)cm^(-2))的金纳米电极阵列,纳米电极分布规则,尺寸高度均一。我们将该金纳米电极阵列作为双极电极阵列,可将电极一侧的电化学法拉第信号在另... 本文制备了嵌于多孔阳极氧化铝(AAO)膜中直径为200 nm,间距为450 nm的高密度(5.7×10^(8)cm^(-2))的金纳米电极阵列,纳米电极分布规则,尺寸高度均一。我们将该金纳米电极阵列作为双极电极阵列,可将电极一侧的电化学法拉第信号在另一侧电极上转化成电致化学发光(ECL)信号,从而实现对单个铂纳米颗粒上氢气析出反应(HER)进行亚微米空间分辨率的电化学成像。本文介绍的方法为高空间分辨率成像电催化材料、能源材料以及细胞过程的局部电化学活性提供了一个良好的平台。 展开更多
关键词 纳米电极阵列 双极电极 电致化学发光成像 电化学成像 单个铂纳米颗粒 氢气析出反应
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Study on the multiple characteristics of M3 generation of pea mutants obtained by neutron irradiation 被引量:1
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作者 Da-Peng Xu Ze-En Yao +3 位作者 jian-bin pan Hu-Yuan Feng Zhi-Qi Guo Xiao-Long Lu 《Nuclear Science and Techniques》 SCIE CAS CSCD 2020年第7期46-54,共9页
Irradiation breeding is an important technique in the effort to solve food shortages and improve the quality of agricultural products.In this study,a field test was implemented on the M3 generation of two mutant pea p... Irradiation breeding is an important technique in the effort to solve food shortages and improve the quality of agricultural products.In this study,a field test was implemented on the M3 generation of two mutant pea plants gained from previous neutron radiation of pea seeds.The relationship between agronomic characteristics and yields of the mutants was investigated.Moreover,differences in physiological and biochemical properties and seed nutrients were analyzed.The results demonstrated that the plant height,effective pods per plant,and yield per plant of mutant Leaf-M1 were 45.0%,43.2%,and 50.9%higher than those of the control group,respectively.Further analysis attributed the increase in yield per plant to the increased branching number.The yield per plant of mutant Leaf-M2 was 7.8%higher than that of the control group,which could be related to the increased chlorophyll content in the leaves.There was a significant difference between the two mutants in the increase in yield per plant owing to morphological variation between the two mutants.There were significant differences in SOD activity and MDA content between the two mutants and the control,indicating that the physiological regulation of the two mutants also changed.In addition,the iron element content of seeds of the two mutants was about 10.9%lower than in the seeds of the control group,a significant difference.These findings indicate that the mutants Leaf-M1 and Leaf-M2 have breeding value and material value for molecular biological studies. 展开更多
关键词 BREEDING attributed gained
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Radiation dose effects on the morphological development of M_(1) generation pea(Pisum sativum)
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作者 Da-Peng Xu Hu-Yuan Feng +2 位作者 jian-bin pan Ze-En Yao Jun-Run Wang 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2021年第11期63-76,共14页
We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose ... We irradiated pea seeds with neutrons from a ^(252)Cf source and studied the radiation dose effects on various morphological development parameters during the growth of M_(1) generation peas.We found that in the dose range of 0.51-9.27 Gy,with the increase in neutron-absorbed dose,the morphological development parameters of M_(1) generation peas at the initial seedling stage showed an obvious trend with three fluctuations.With the development of pea,this trend gradually weakened.Further analysis and verification showed that the main trend in the M_(1) generation of pea seeds was an inhibitory effect induced by neutron irradiation and there was a good linear correlation between the inhibitory effect and neutron absorption dose We successfully demonstrated the background removal of mutant plants and defined morphological developmen parameters for peas that match the overall development of plants.Our results will positively impact neutron mutation breeding and automatic agriculture. 展开更多
关键词 Neutron irradiation Pea(Pisum sativum) ^(252)Cf source Radiation does effects Absorbed dose Morphological development
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Highly Efficient Near-Infrared Ⅱ Electrochemiluminescence from NaYbF_(4) Core Mesoporous Silica Shell Nanoparticles
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作者 Si-Yuan Ji jian-bin pan +3 位作者 Hao-Zhi Wang Wei Zhao Hong-Yuan Chen Jing-Juan Xu 《CCS Chemistry》 CAS 2022年第9期3076-3083,共8页
Due to less interference in biological imaging,nanomaterials with second near-infrared(NIR-II)window(950–1700 nm)emission have received tremendous attention.However,no reports on NIR-Ⅱ electrochemiluminescence(ECL)i... Due to less interference in biological imaging,nanomaterials with second near-infrared(NIR-II)window(950–1700 nm)emission have received tremendous attention.However,no reports on NIR-Ⅱ electrochemiluminescence(ECL)imaging exist because of the lack of high-efficiency NIR-Ⅱ ECL luminophores.Herein,we designed and synthesized a NaYbF4@SiO_(2) core–shell nanoparticle for the first time. 展开更多
关键词 near-infraredⅡ ELECTROCHEMILUMINESCENCE lanthanide nanoparticles mesoporous silica core–shell structure
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