Inflammatory bowel disease(IBD)represents a chronic inflammatory condition profoundly impacting the gastrointestinal tract.Its prevalence has markedly risen in both developed and developing nations over recent decades...Inflammatory bowel disease(IBD)represents a chronic inflammatory condition profoundly impacting the gastrointestinal tract.Its prevalence has markedly risen in both developed and developing nations over recent decades.Despite the absence of definitive etiological elucidation,therapeutic strategies predominantly revolve around pharmacological interventions aimed at symptom mitigation.Alginate(AG)is a polysaccharide of marine origin that has garnered significant attention due to its inherent biocompatibility,pH sensitivity,and cross-linking.Its exploration within drug delivery systems for IBD treatment stems from its natural sourcing,non-cytotoxic nature,and economic viability.Notably,AG demonstrates facile interpolymeric cross-linking,facilitating the formation of a cohesive network conducive to sustained drug release kinetics.AG-based carrier systems for sustained drug release,and targeted drug delivery have been widely studied.This article reviews the pathogenesis of IBD and the current drugs,AG-based drug delivery systems and their properties in alleviating IBD.The prospect of further development of AG in the field of biopharmaceutical and drug delivery is prospected.展开更多
AIM:To determine if TSPAN1 overexpression is associated with clinicopathological and prognostic factors in human colorectal adenocarcinoma.METHODS:Total RNA was extracted in 20 human adenocarcinoma tissues for TSPAN1 ...AIM:To determine if TSPAN1 overexpression is associated with clinicopathological and prognostic factors in human colorectal adenocarcinoma.METHODS:Total RNA was extracted in 20 human adenocarcinoma tissues for TSPAN1 mRNA assay by RT-PCR.Eighty-eight specimens of human colorectal adenocarcinoma were surgically removed.TSPAN1 protein levels in cancer tissues were determined by immunohistochemistry using a polyclonal antibody against self-prepared TSPAN1.The correlation between TSPAN1 expression and the clinicopathological factors and the overall survival rate was analyzed by univariate and multivariate assay.RESULTS:TSPAN1 mRNA was detected in 90.0%(18/20) of cancerous tissues.The light density of TSPAN1 mRNA expression levels was 0.89 ± 0.30 in adenocarcinoma by gel-image system.TSPAN1 protein expression was detected in 78.41%(69/88) and weakly expressed in 40% normal colorectal tissues.There were significant differences between colorectal adenocarcinoma and normal control epithelium(P < 0.05).TSPAN1 protein expression in colorectal cancerous tissue was significantly correlated with the histological grade,cell expression PCNA,lymph nodal metastasis and TNM staging of the disease.Patients with TSPAN1 protein overexpression had a significantly shorter survival period than that in patients with TSPAN1 protein negative or weak expression,respectively(P < 0.05).Furthermore,by multivariate analysis,TSPAN1 protein expression demonstrated an independent prognostic factor for human colorectal cancers(P < 0.05,relative risk 0.755;95% confidence interval 0.302-1.208).CONCLUSION:The expression of TSPAN1 gene is increased in colorectal carcinoma,suggesting that TSPAN1 might serve as an independent prognostic factor for the colorectal adenocarcinoma patients.展开更多
Helicobacter pylori(H.pylori)is one of the most common pathogenic bacterial infections and is found in the stomachs of approximately half of the world’s population.It is the primary known cause of gastritis,gastroduo...Helicobacter pylori(H.pylori)is one of the most common pathogenic bacterial infections and is found in the stomachs of approximately half of the world’s population.It is the primary known cause of gastritis,gastroduodenal ulcer disease and gastric cancer.However,combined drug therapy as the general treatment in the clinic,the rise of antibiotic-resistant bacteria,adverse reactions and poor patient compliance are major obstacles to the eradication of H.pylori.Oral site-specific drug delivery systems that could increase the longevity of the treatment agent at the target site might improve the therapeutic effect and avoid side effects.Gastroretentive drug delivery systems potentially prolong the gastric retention time and controlled/sustained release of a drug,thereby increasing the concentration of the drug at the application site,potentially improving its bioavailability and reducing the necessary dosage.Recommended gastroretentive drug delivery systems for enhancing local drug delivery include floating systems,bioadhesive systems and expandable systems.In this review,we summarize the important physiological parameters of the gastrointestinal tract that affect the gastric residence time.We then focus on various aspects useful in the development of gastroretentive drug delivery systems,including current trends and the progress of novel forms,especially with respect to their application for the treatment of H.pylori infections.展开更多
BACKGROUND Portal vein thrombosis(PVT)was previously a contraindication for trans-jugular intrahepatic portosystemic shunt(TIPS).AIM To perform a systematic review and meta-analysis of the current available studies in...BACKGROUND Portal vein thrombosis(PVT)was previously a contraindication for trans-jugular intrahepatic portosystemic shunt(TIPS).AIM To perform a systematic review and meta-analysis of the current available studies investigating outcomes of TIPS for cirrhotic patient with PVT.METHODS Multiple databases were systematically searched to identify studies investigating the outcomes of TIPS for cirrhotic patients with PVT.The quality of studies was assessed by Cochrane Collaboration method and Methodological Index for Non-Randomized Studies.The demographic data,outcomes,combined treatment,and anticoagulation strategy were extracted.RESULTS Twelve studies were identified with 460 patients enrolled in the analysis.The technical success rate was 98.9%in patients without portal vein cavernous transformation and 92.3%in patients with portal vein cavernous transformation.One-year portal vein recanalization rate was 77.7%,and TIPS patency rate was 84.2%.The cumulative encephalopathy rate was 16.4%.One-year overall survival was 87.4%.CONCLUSION TIPS is indicated for portal hypertension related complications and the restoration of pre-transplantation portal vein patency in cirrhotic patients with PVT.Cavernous transfor-mation is an indicator for technical failure.Post-TIPS anticoagulation seems not mandatory.Simultaneous TIPS and percutaneous mechanical thrombectomy device could achieve accelerated portal vein recanalization and decreased thrombolysis-associated complications,but further investigation is still needed.展开更多
Gastrointestinal(GI)cancer is one of the leading causes of cancer-related deaths worldwide.According to the Global Cancer Statistics,colorectal cancer is the second leading cause of cancer-related mortality,closely fo...Gastrointestinal(GI)cancer is one of the leading causes of cancer-related deaths worldwide.According to the Global Cancer Statistics,colorectal cancer is the second leading cause of cancer-related mortality,closely followed by gastric cancer(GC).Environmental,dietary,and lifestyle factors including cigarette smoking,alcohol intake,and genetics are the most important risk factors for GI cancer.Furthermore,infections caused by Helicobacter pylori are a major cause of GC initiation.Despite improvements in conventional therapies,including surgery,chemotherapy,and radiotherapy,the length or quality of life of patients with advanced GI cancer is still poor because of delayed diagnosis,recurrence and side effect.Resveratrol(3,4,5-trihydroxy-trans-stilbene;Res),a natural polyphenolic compound,reportedly has various pharmacologic functions including anti-oxidant,anti-inflammatory,anti-cancer,and cardioprotective functions.Many studies have demonstrated that Res also exerts a chemopreventive effect on GI cancer.Research investigating the anti-cancer mechanism of Res for the prevention and treatment of GI cancer has implicated multiple pathways including oxidative stress,cell proliferation,and apoptosis.Therefore,this paper provides a review of the function and molecular mechanisms of Res in the prevention and treatment of GI cancer.展开更多
BACKGROUND Acute carotid stent thrombosis(ACST)is a rare but devastating complication in the carotid artery stenting(CAS)procedure.The aim of this article is to report a case and review cases of ACST reported in the l...BACKGROUND Acute carotid stent thrombosis(ACST)is a rare but devastating complication in the carotid artery stenting(CAS)procedure.The aim of this article is to report a case and review cases of ACST reported in the literature,and investigate risk factors and management strategies for ACST.CASE SUMMARY We reviewed the treatment process of a patient with ACST after CAS.Then multiple databases were systematically searched to identify studies reporting ACST from 2005 to 2020.The demographic data,risk factors,treatment strategies,and prognosis were extracted and analyzed.CONCLUSION The reason for ACST is multifactorial.Proper patient selection,normative antiplatelet treatment,and perfect technical detail may decrease the incidence of ACST.Several treatment strategies such as thrombolysis,mechanical thrombectomy,and open surgery may be options for the treatment of ACST.Limited data have shown that carotid endarterectomy is effective with favorable results.展开更多
The surface morphology of free-surface PCL ringed spherulites was investigated by using atomic force microscopy. The spherulites were obtained by crystallization of PCL/PVC blends of different compositions. It was fou...The surface morphology of free-surface PCL ringed spherulites was investigated by using atomic force microscopy. The spherulites were obtained by crystallization of PCL/PVC blends of different compositions. It was found that the ringed spherulite exhibited regularly fluctuating rings on its surface. Compared with the bright-dark ring pattern of the spherulite under a polarizing microscope, it was proved that the optical characteristics of the ringed spherulite under polarizing microscope coincided with its surface characteristics. The bright rings in polarizing micrographs of the spherulite coincided with the convex rings on its surface, while the dark rings coincided with the concave rings.展开更多
Background and Aims:Prolyl endopeptidase(PREP)is a serine endopeptidase that participates in many pathological processes including inflammation,oxidative stress,and autophagy.Our previous studies found that PREP knock...Background and Aims:Prolyl endopeptidase(PREP)is a serine endopeptidase that participates in many pathological processes including inflammation,oxidative stress,and autophagy.Our previous studies found that PREP knockout exhibited multiple benefits in high-fat diet(HFD)or methionine choline-deficient diet-induced metabolic dysfunctionassociated fatty liver disease(MAFLD).However,cumulative studies have suggested that PREP performs complex functions during disease development.Therefore,further understanding the role of PREP in MAFLD development is the foundation of PREP intervention.Methods:In this study,an HFD-induced MAFLD model at different time points(4,8,12,and 16 weeks)was used to explore dynamic changes in the PREP proline-glycine-proline(PGP)/N-acetyl-seryl-aspartyllysyl-proline(AcSDKP)system.To explore its potential value in MAFLD treatment,saline,or the PREP inhibitor,KYP-2047,was administered to HFD-induced MAFLD mice from the 10th to 16th weeks.Results:PREP activity and expression were increased in HFD-mice compared with control mice from the 12th week onwards,and increased PREP mainly resulted in the activation of the matrix metalloproteinase 8/9(MMP8/9)-PREP-PGP axis rather than the thymosin B4-meprin a/PREP-AcSDKP axis.In addition,KYP-2047 reduced HFD-induced liver injury and oxidative stress,improved lipid metabolism through the suppression of lipogenic genes and the induction of B-oxidation-related genes,and attenuated hepatic inflam-mation by decreasing MMP8/9 and PGP.Moreover,KYP2047 restored HFD-induced impaired autophagy and this was veri-fied in HepG2 cells.Conclusions:These findings suggest that increased PREP activity/expression during MAFLD de-velopment might be a key factor in the transition from sim-ple steatosis to steatohepatitis,and KYP-2047 might possess therapeutic potential for MAFLD treatment.展开更多
Background: Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. Cell transplantation has great therapeutic potential for endothelial recovery. We investigated...Background: Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. Cell transplantation has great therapeutic potential for endothelial recovery. We investigated the effect of transplanting endothelial progenitor cells (EPCs) derived from human early fetal aortas in rat injured arteries. Methods: The carotid arterial endothelium of Sprague-Dawley rats was damaged by dilatation with a 1.5 F balloon catheter, and then EPCs derived from human early fetal aortas (〈14 weeks) were injected into the lumen of the injured artery in transplanted rats, with an equal volume of normal saline injected into control rats. Rats were sacrificed at 2 and 4 weeks after treatment and transplanted cells were identified by immunohistochemical staining with anti-human CD31 and anti-human mitochondria antibodies. Arterial cross-sections were analyzed by pathology, immunohistochemistry, and morphometry. Results: Green fluorescence-labeled EPCs could be seen in the endovascular surface of balloon-injured vessels after transplantation. The intimal area and intimal/medial area ratio were significantly smaller in the transplanted group than in the control (P 〈 0.05) and the residual lumen area was larger (P 〈 0.05). After EPC transplantation, a complete vascular endothelial layer was formed, which was positive for human yon Willebrand factor after immunohistochemical staining, and immunohistochemical staining revealed many CD31- and mitochondria-positive cells in the re-endothelialized endothelium with EPC transplantation but not control treatment. Conclusion: EPCs derived from human early fetal aorta were successfully transplanted into injured vessels and might inhibit neointimal hyperplasia after vascular injury.展开更多
In this work, magnesium tetraphenylporphyrin (MgTPP) was used as a new supramolecular amine-fixing agent. Once introduced, C02 easily competes with MgTPP for amines, leading to the release of MgTPP. The processes ca...In this work, magnesium tetraphenylporphyrin (MgTPP) was used as a new supramolecular amine-fixing agent. Once introduced, C02 easily competes with MgTPP for amines, leading to the release of MgTPP. The processes can be explained by the fact that the association constant (Kassoc) values of MgTPP with amines were in the range of 0.6 (ethanolamine) to 3.9 (ethylenediamine), which are lower than the Kassoc values of C02 with these amines. MgTPP interacted with aniline, ethanolamine, pyrrolidine, or ethylenediamine to form 1:1 adducts. Ethylenediamine presents a stronger Kassoc value for MgTPP, so it was considered an optimal agent for C02 capture.展开更多
Xiamen Xiangan Subsea Tunnel is the first undersea tunnel constructed in China. It has become the major undersea pathway connecting Xiamen Island and Xiangan District since its operation in 2010. The total length is 6...Xiamen Xiangan Subsea Tunnel is the first undersea tunnel constructed in China. It has become the major undersea pathway connecting Xiamen Island and Xiangan District since its operation in 2010. The total length is 6.05 km with the undersea length of 4.2 km. In an effort to onsite-monitor reinforcement corrosion in concrete structures, the commercially available CorroWatch multiprobe sensors and ERE-20 reference electrodes were pre-embedded in the selected locations and positions of supporting structures during the tunnel constructions. The real-time data have been collected annually by onsite measurements for 6 years. In this paper, the feasibility of the onsite corrosion monitoring system and suitability of the measured parameters including corrosion current, potential and temperature are discussed based on the preliminary results. The measured typical cyclic-type variations in corrosion current and temperature with monitoring time might be related to the seasonal changes during the annual routine measurements. The widely scattered corrosion potentials that are fluctuated complicatedly reflected localized differences in the vicinity of rebar and concrete. These findings, along with the progressively ongoing research, will provide valuable information in structural durability for service life prediction of reinforced concrete structures exposed to marine environments.展开更多
基金supported by the China Postdoctoral Science Foundation(Grant No.2021MD703801)Youth Talent Cultivation Fund Project of Dalian Medical University(Grant No.508021)Open Foundation of the State Key Laboratory of Marine Food Processing&Safety Control(Grant No.SKL2023M03).
文摘Inflammatory bowel disease(IBD)represents a chronic inflammatory condition profoundly impacting the gastrointestinal tract.Its prevalence has markedly risen in both developed and developing nations over recent decades.Despite the absence of definitive etiological elucidation,therapeutic strategies predominantly revolve around pharmacological interventions aimed at symptom mitigation.Alginate(AG)is a polysaccharide of marine origin that has garnered significant attention due to its inherent biocompatibility,pH sensitivity,and cross-linking.Its exploration within drug delivery systems for IBD treatment stems from its natural sourcing,non-cytotoxic nature,and economic viability.Notably,AG demonstrates facile interpolymeric cross-linking,facilitating the formation of a cohesive network conducive to sustained drug release kinetics.AG-based carrier systems for sustained drug release,and targeted drug delivery have been widely studied.This article reviews the pathogenesis of IBD and the current drugs,AG-based drug delivery systems and their properties in alleviating IBD.The prospect of further development of AG in the field of biopharmaceutical and drug delivery is prospected.
基金Supported by The University High-New-Tech Development Fund of Jiangsu Province,No. JHO2-118Natural Science Foundation of Jiangsu Province,No.BK2006058National Natural and Science Foundation,No.30771126
文摘AIM:To determine if TSPAN1 overexpression is associated with clinicopathological and prognostic factors in human colorectal adenocarcinoma.METHODS:Total RNA was extracted in 20 human adenocarcinoma tissues for TSPAN1 mRNA assay by RT-PCR.Eighty-eight specimens of human colorectal adenocarcinoma were surgically removed.TSPAN1 protein levels in cancer tissues were determined by immunohistochemistry using a polyclonal antibody against self-prepared TSPAN1.The correlation between TSPAN1 expression and the clinicopathological factors and the overall survival rate was analyzed by univariate and multivariate assay.RESULTS:TSPAN1 mRNA was detected in 90.0%(18/20) of cancerous tissues.The light density of TSPAN1 mRNA expression levels was 0.89 ± 0.30 in adenocarcinoma by gel-image system.TSPAN1 protein expression was detected in 78.41%(69/88) and weakly expressed in 40% normal colorectal tissues.There were significant differences between colorectal adenocarcinoma and normal control epithelium(P < 0.05).TSPAN1 protein expression in colorectal cancerous tissue was significantly correlated with the histological grade,cell expression PCNA,lymph nodal metastasis and TNM staging of the disease.Patients with TSPAN1 protein overexpression had a significantly shorter survival period than that in patients with TSPAN1 protein negative or weak expression,respectively(P < 0.05).Furthermore,by multivariate analysis,TSPAN1 protein expression demonstrated an independent prognostic factor for human colorectal cancers(P < 0.05,relative risk 0.755;95% confidence interval 0.302-1.208).CONCLUSION:The expression of TSPAN1 gene is increased in colorectal carcinoma,suggesting that TSPAN1 might serve as an independent prognostic factor for the colorectal adenocarcinoma patients.
文摘Helicobacter pylori(H.pylori)is one of the most common pathogenic bacterial infections and is found in the stomachs of approximately half of the world’s population.It is the primary known cause of gastritis,gastroduodenal ulcer disease and gastric cancer.However,combined drug therapy as the general treatment in the clinic,the rise of antibiotic-resistant bacteria,adverse reactions and poor patient compliance are major obstacles to the eradication of H.pylori.Oral site-specific drug delivery systems that could increase the longevity of the treatment agent at the target site might improve the therapeutic effect and avoid side effects.Gastroretentive drug delivery systems potentially prolong the gastric retention time and controlled/sustained release of a drug,thereby increasing the concentration of the drug at the application site,potentially improving its bioavailability and reducing the necessary dosage.Recommended gastroretentive drug delivery systems for enhancing local drug delivery include floating systems,bioadhesive systems and expandable systems.In this review,we summarize the important physiological parameters of the gastrointestinal tract that affect the gastric residence time.We then focus on various aspects useful in the development of gastroretentive drug delivery systems,including current trends and the progress of novel forms,especially with respect to their application for the treatment of H.pylori infections.
文摘BACKGROUND Portal vein thrombosis(PVT)was previously a contraindication for trans-jugular intrahepatic portosystemic shunt(TIPS).AIM To perform a systematic review and meta-analysis of the current available studies investigating outcomes of TIPS for cirrhotic patient with PVT.METHODS Multiple databases were systematically searched to identify studies investigating the outcomes of TIPS for cirrhotic patients with PVT.The quality of studies was assessed by Cochrane Collaboration method and Methodological Index for Non-Randomized Studies.The demographic data,outcomes,combined treatment,and anticoagulation strategy were extracted.RESULTS Twelve studies were identified with 460 patients enrolled in the analysis.The technical success rate was 98.9%in patients without portal vein cavernous transformation and 92.3%in patients with portal vein cavernous transformation.One-year portal vein recanalization rate was 77.7%,and TIPS patency rate was 84.2%.The cumulative encephalopathy rate was 16.4%.One-year overall survival was 87.4%.CONCLUSION TIPS is indicated for portal hypertension related complications and the restoration of pre-transplantation portal vein patency in cirrhotic patients with PVT.Cavernous transfor-mation is an indicator for technical failure.Post-TIPS anticoagulation seems not mandatory.Simultaneous TIPS and percutaneous mechanical thrombectomy device could achieve accelerated portal vein recanalization and decreased thrombolysis-associated complications,but further investigation is still needed.
基金Supported by National Natural Science Foundation of China,No.21576254National Natural Science Foundation of China,No.81903560Dalian Young Star of Science and Technology Project,No.2018RQ81.
文摘Gastrointestinal(GI)cancer is one of the leading causes of cancer-related deaths worldwide.According to the Global Cancer Statistics,colorectal cancer is the second leading cause of cancer-related mortality,closely followed by gastric cancer(GC).Environmental,dietary,and lifestyle factors including cigarette smoking,alcohol intake,and genetics are the most important risk factors for GI cancer.Furthermore,infections caused by Helicobacter pylori are a major cause of GC initiation.Despite improvements in conventional therapies,including surgery,chemotherapy,and radiotherapy,the length or quality of life of patients with advanced GI cancer is still poor because of delayed diagnosis,recurrence and side effect.Resveratrol(3,4,5-trihydroxy-trans-stilbene;Res),a natural polyphenolic compound,reportedly has various pharmacologic functions including anti-oxidant,anti-inflammatory,anti-cancer,and cardioprotective functions.Many studies have demonstrated that Res also exerts a chemopreventive effect on GI cancer.Research investigating the anti-cancer mechanism of Res for the prevention and treatment of GI cancer has implicated multiple pathways including oxidative stress,cell proliferation,and apoptosis.Therefore,this paper provides a review of the function and molecular mechanisms of Res in the prevention and treatment of GI cancer.
基金Supported by Elite Medical Professionals Project of China-Japan Friendship Hospital,No.ZRJY2021-QM13。
文摘BACKGROUND Acute carotid stent thrombosis(ACST)is a rare but devastating complication in the carotid artery stenting(CAS)procedure.The aim of this article is to report a case and review cases of ACST reported in the literature,and investigate risk factors and management strategies for ACST.CASE SUMMARY We reviewed the treatment process of a patient with ACST after CAS.Then multiple databases were systematically searched to identify studies reporting ACST from 2005 to 2020.The demographic data,risk factors,treatment strategies,and prognosis were extracted and analyzed.CONCLUSION The reason for ACST is multifactorial.Proper patient selection,normative antiplatelet treatment,and perfect technical detail may decrease the incidence of ACST.Several treatment strategies such as thrombolysis,mechanical thrombectomy,and open surgery may be options for the treatment of ACST.Limited data have shown that carotid endarterectomy is effective with favorable results.
基金ACKNOWLEDGMENTS This work was supported by the "Western Light" Visiting Scholar Plan, the Program for New Century Excellent Talents in University (No.NCET-12-1017), the Program for Grassland Excellent Talents of Inner Mongolia Autonomous Region, the Inner Mengolia Science Technology Plan, the Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region (No.NJYT-12-B13), the Natural Science Foundation of Inner Mongolia Autonomous Region (No.2011BS0601, China), the Inner Mongolia Talented People Development Fund, and Yongfeng Boyuan Industry Co., Ltd. Jiangxi Province, China.
文摘铈(III ) tetraphenylporphyrin 硝酸盐 Ce (TPP ) NO3 被使用 mesotetraphenylporphyrin (TPP ) 和 Ce (NO3 ) 综合敭瑮污瀠潲散吗?
基金This work was supported by The National Natural Science Foundation of China and also The State Science and Technology Commission of China under The National Key Project for Fundamental Research "Macromolecular Condensed State".
文摘The surface morphology of free-surface PCL ringed spherulites was investigated by using atomic force microscopy. The spherulites were obtained by crystallization of PCL/PVC blends of different compositions. It was found that the ringed spherulite exhibited regularly fluctuating rings on its surface. Compared with the bright-dark ring pattern of the spherulite under a polarizing microscope, it was proved that the optical characteristics of the ringed spherulite under polarizing microscope coincided with its surface characteristics. The bright rings in polarizing micrographs of the spherulite coincided with the convex rings on its surface, while the dark rings coincided with the concave rings.
基金supported by grants from the National Natural Science Foundation of China(81970511,82270620).
文摘Background and Aims:Prolyl endopeptidase(PREP)is a serine endopeptidase that participates in many pathological processes including inflammation,oxidative stress,and autophagy.Our previous studies found that PREP knockout exhibited multiple benefits in high-fat diet(HFD)or methionine choline-deficient diet-induced metabolic dysfunctionassociated fatty liver disease(MAFLD).However,cumulative studies have suggested that PREP performs complex functions during disease development.Therefore,further understanding the role of PREP in MAFLD development is the foundation of PREP intervention.Methods:In this study,an HFD-induced MAFLD model at different time points(4,8,12,and 16 weeks)was used to explore dynamic changes in the PREP proline-glycine-proline(PGP)/N-acetyl-seryl-aspartyllysyl-proline(AcSDKP)system.To explore its potential value in MAFLD treatment,saline,or the PREP inhibitor,KYP-2047,was administered to HFD-induced MAFLD mice from the 10th to 16th weeks.Results:PREP activity and expression were increased in HFD-mice compared with control mice from the 12th week onwards,and increased PREP mainly resulted in the activation of the matrix metalloproteinase 8/9(MMP8/9)-PREP-PGP axis rather than the thymosin B4-meprin a/PREP-AcSDKP axis.In addition,KYP-2047 reduced HFD-induced liver injury and oxidative stress,improved lipid metabolism through the suppression of lipogenic genes and the induction of B-oxidation-related genes,and attenuated hepatic inflam-mation by decreasing MMP8/9 and PGP.Moreover,KYP2047 restored HFD-induced impaired autophagy and this was veri-fied in HepG2 cells.Conclusions:These findings suggest that increased PREP activity/expression during MAFLD de-velopment might be a key factor in the transition from sim-ple steatosis to steatohepatitis,and KYP-2047 might possess therapeutic potential for MAFLD treatment.
文摘Background: Endothelial cell damage is an important pathophysiological step of restenosis after angioplasty and stenting. Cell transplantation has great therapeutic potential for endothelial recovery. We investigated the effect of transplanting endothelial progenitor cells (EPCs) derived from human early fetal aortas in rat injured arteries. Methods: The carotid arterial endothelium of Sprague-Dawley rats was damaged by dilatation with a 1.5 F balloon catheter, and then EPCs derived from human early fetal aortas (〈14 weeks) were injected into the lumen of the injured artery in transplanted rats, with an equal volume of normal saline injected into control rats. Rats were sacrificed at 2 and 4 weeks after treatment and transplanted cells were identified by immunohistochemical staining with anti-human CD31 and anti-human mitochondria antibodies. Arterial cross-sections were analyzed by pathology, immunohistochemistry, and morphometry. Results: Green fluorescence-labeled EPCs could be seen in the endovascular surface of balloon-injured vessels after transplantation. The intimal area and intimal/medial area ratio were significantly smaller in the transplanted group than in the control (P 〈 0.05) and the residual lumen area was larger (P 〈 0.05). After EPC transplantation, a complete vascular endothelial layer was formed, which was positive for human yon Willebrand factor after immunohistochemical staining, and immunohistochemical staining revealed many CD31- and mitochondria-positive cells in the re-endothelialized endothelium with EPC transplantation but not control treatment. Conclusion: EPCs derived from human early fetal aorta were successfully transplanted into injured vessels and might inhibit neointimal hyperplasia after vascular injury.
基金supported by the National Natural Science Foundation of China(No.21166017)the Research Fund for the Doctoral Program of Higher Education of China(No. 20111514120002)+3 种基金the Natural Science Foundation of Inner Mongolia Autonomous Region(No.2011B50601)Inner Mongolia Autonomous Region's Educational Commission(No.NJZZ11068)the Inner Mongolia Talented People Development Fund,Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region(No.NJYT-12-B13)Program for New Century Excellent Talents in University(NCET-12-1017)
文摘In this work, magnesium tetraphenylporphyrin (MgTPP) was used as a new supramolecular amine-fixing agent. Once introduced, C02 easily competes with MgTPP for amines, leading to the release of MgTPP. The processes can be explained by the fact that the association constant (Kassoc) values of MgTPP with amines were in the range of 0.6 (ethanolamine) to 3.9 (ethylenediamine), which are lower than the Kassoc values of C02 with these amines. MgTPP interacted with aniline, ethanolamine, pyrrolidine, or ethylenediamine to form 1:1 adducts. Ethylenediamine presents a stronger Kassoc value for MgTPP, so it was considered an optimal agent for C02 capture.
基金financial support and onsite assistance from the Xiamen Road and Bridge Construction Group Co.,Ltd.,China
文摘Xiamen Xiangan Subsea Tunnel is the first undersea tunnel constructed in China. It has become the major undersea pathway connecting Xiamen Island and Xiangan District since its operation in 2010. The total length is 6.05 km with the undersea length of 4.2 km. In an effort to onsite-monitor reinforcement corrosion in concrete structures, the commercially available CorroWatch multiprobe sensors and ERE-20 reference electrodes were pre-embedded in the selected locations and positions of supporting structures during the tunnel constructions. The real-time data have been collected annually by onsite measurements for 6 years. In this paper, the feasibility of the onsite corrosion monitoring system and suitability of the measured parameters including corrosion current, potential and temperature are discussed based on the preliminary results. The measured typical cyclic-type variations in corrosion current and temperature with monitoring time might be related to the seasonal changes during the annual routine measurements. The widely scattered corrosion potentials that are fluctuated complicatedly reflected localized differences in the vicinity of rebar and concrete. These findings, along with the progressively ongoing research, will provide valuable information in structural durability for service life prediction of reinforced concrete structures exposed to marine environments.