Pd nanoparticles immobilized on MIL-53(Al)as highly e ective bifunctional catalysts for oxidation of liquid methanol to methyl formate

A series of Pd/MIL-53(Al) heterogeneous bifunctional catalysts with di erent Pd contents were prepared by an impregnation method. The prepared metal–organic frameworks MIL-53(Al) and catalysts were characterized by XRD, SEM, HRTEM,FT-IR and N2 adsorption/desorption techniques. The results showed that MIL-53(Al) was synthesized successfully, and the structure was unchanged during and after the preparation of the catalysts. The Pd nanoparticles(NPs) with an average particle size of 4.6 nm were uniformly dispersed on the MIL-53(Al). The catalyst exhibited good catalytic activity in the selective oxidation of liquid methanol to methyl formate. Under the conditions of 150 °C, 2 MPa O2 and solvent-free for5 h, the conversion of methanol could reach 60.3%, and the selectivity of methyl formate was up to 62.2%. In addition, the Pd/MIL-53(Al) bifunctional catalyst exhibited excellent stability and maintained high catalytic activity after five cycles.

Novel computerized psychometric tests as primary screening tools for the diagnosis of minimal hepatic encephalopathy

Minimal hepatic encephalopathy(MHE)is a critical neurocognitive complication of decompensated liver cirrhosis and portosystemic shunting,which results in a wide range of cognitive deficits including impairments in working attention,psychomotor speed,and executive function.Current guidelines have recommended paper-and-pencil psychometric tests for the diagnosis of MHE.Most high-risk cirrhotic patients are required to be examined;however,paperand-pencil psychometric tests are neither convenient nor rapid to perform in the clinic.Recently,novel computerized psychometric tests,including the inhibitory control test,EncephalApp Stroop App,and critical flicker frequency,have been proven to be rapid,effective,and convenient methods for screening MHE in clinical practice and for identifying high-risk cirrhotic patients for further validation using rigid neuropsychometric examinations.However,diagnostic accuracy of these tests is influenced by educational background,age,and cultural differences.This review summarizes clinical evidence of the application of novel computerized psychometric tests for screening MHE.

Transcriptome sequencing analysis of lncRNA expression in peripheral blood mononuclear cells from patients with COPD

Objective:To screen abnormally expressed lncRNAs in peripheral blood mononuclear cells from patients with COPD.Methods:The peripheral blood of 3 COPD patients and 3 normal controls were collected from our hospital,mononuclear cells were isolated,RNA was extracted and then transcriptome sequencing was performed.The expression difference between the two groups of samples was calculated based on p<0.05 and|log2FC|>1.Plot the difference lncRNA heat map and volcano map.The Lncpro database may predict mRNAs regulated by differential lncRNA,and perform the GO function and KEGG signaling clustering.Results:There were 67 lncRNAs between the COPD group and the control group that met the difference of p<0.05 and|log2FC|>1,of which 33 were up-regulated and 34 were down-regulated.Between the two groups.The target genes are mainly enriched in GO functions:regulatory functions of multicellular biological processes,regulatory functions of development processes,structured morphogenesis functions,system development functions,and development process functions.Target genes are mainly enriched in KEGG signaling pathways:multi-species apoptotic pathway,TGF-βsignaling pathway,complement and coagulation cascade pathway,colorectal cancer pathway and apoptosis pathway.Conclusion:Our results provide general information and possible regulatory functions and pathways of lncRNA expression changes in peripheral blood mononuclear cells of COPD,which may help clarify the underlying mechanism of COPD.

Expression and clinical significance of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease

Objective:To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease,and to explore its clinical significance with chronic obstructive pulmonary disease.Methods:The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital were collected.Extraction of exosomes and electron microscopy to identify the characteristics of exosomes.RT-PCR method was used to detect the miR-186 expression level in serum exosomes.And statistical analysis of the expression difference between the COPD group and the normal group,alysis of the correlation between miR-186 and FEV1%predicted value,and ROC curve analysis of the diagnostic value of serum exosome miR-186 in COPD.Results:The relative expression of miR-186 in serum exosomes of COPD group was 3.24±0.14,which was significantly higher than that of normal control group 1.03±0.04(t=15.47,P<0.001).The expression level of miR-186 was negatively correlated with the predicted FEV1%of COPD patients(r=-0.5024,p<0.0001).The area under the ROC curve between miR-186 and COPD patients was 0.8409(95%confidence interval:0.6875-0.9943).Conclusion:The high expression of miR-186 in serum exosomes may be a risk factor and may be used as a diagnostic indicator of COPD.

Expression and clinical significance of lncRNA SNHG3 in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease

Objective: To investigate the expression of lncRNA SNHG3 in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease (COPD), and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: Mononuclear cells were collected from 120 patients with COPD and 110 normal controls. The expression of lncRNA SNHG3 in peripheral blood mononuclear cells was detected by RT-PCR and the difference between COPD group and normal group, and the difference between mild, moderate, severe, extremely severe COPD and health volunteers was analyzed. Results: The relative expression of SNHG3 in peripheral blood mononuclear cells of COPD patients was 1.52 ± 0.52, which was lower than that of the normal control group (2.51 ± 0.59) (P < 0.001). The relative expression of SNHG3 in peripheral blood mononuclear cells of mild COPD was 2.16 ± 0.23, which was higher than that of moderate group (1.55 ± 0.10) (P < 0.001), severe group 1.19 ± 0.11 (P< 0.001), and extremely severe group 0.89 ± 0.06(P < 0.001). In addition, lncRNA SNHG3 can bind to miR-186 and regulate its expression. Conclusions: The expression of lncRNA SNHG3 in peripheral blood mononuclear cells of COPD patients may be a risk factor for COPD and an indicator of the severity of COPD patients. The lncRNA SNHG3/miR-186 regulatory network may play an important role in the development of COPD.

Mir-141 regulates proliferation and apoptosis of lung fibroblasts by targeting ZEB1

Objective:To study the significance of mir-141 expression in COPD(chronic obstructive pulmonary diseases)mononuclear cells,and to demonstrate its effect on proliferation and apoptosis of fibroblasts Possible to ZEB1(zinc finger E-box binding homeobox 1).Methods:40 cases acute phase of COPD patients,80 cases of stable period COPD patients and 110 normal controls in our hospital were collected.RT-PCR was used to detect mir-141 and statistical analysis.The mir-141 mimic was constructed and transfected into lung fibroblasts.The expression of mir-141 was analyzed by RT-PCR.Cell proliferation was analyzed by CCK8 and cell apoptosis was detected by flow cytometry.Targetscan was used to predict the binding site of ZEB1 and mir-141.The target relationship between ZEB1 and mir-141 was identified by RT-PCR and western blot.Results:The expression of mir-141 in mononuclear cells of acute phase of COPD and stable period of COPD was significantly higher than that of normal controls.Mir-141 levels in acute phase of COPD were significantly higher than that of stable period of COPD.Mir-141 mimic transfection significantly up-regulated the expression of mir-141,promoted cell activity and decreased cell apoptosis rate compared with the empty control group.ZEB1 and mir-141 had binding sites predicted by Targetscan database and verified by dual luciferase assays.After mir-141 mimic transfection hat,ZEB1 mRNA and protein expression were down-regulated.Conclusions:High expression of mir-141 in COPD may promote the proliferation of lung fibroblasts and inhibit apoptosis by targeting ZEB1.

饲喂玉米秸秆粗饲料可加剧奶牛外周血单核细胞释放炎性细胞因子（英文）

目的:研究不同类型的粗饲料对奶牛外周血细胞因子的影响。创新点:明确饲喂玉米秸秆日粮奶牛外周血单核细胞基因的表达特征,研究日粮对外周血细胞因子的影响。方法:分别收集饲喂苜蓿混合粗饲料和玉米秸秆粗饲料的奶牛牛奶、外周血单核细胞及血清,检测牛奶营养含量,应用表达谱基因芯片分析筛选外周血单核细胞中差异表达基因,并采用荧光定量聚合酶链反应(qP CR)验证。最后采用酶联免疫吸附试验(ELISA)检测血清细胞因子的表达量。结论:与苜蓿混合粗饲料组相比较,玉米秸秆粗饲料组的奶牛外周血单核细胞炎性细胞因子分泌水平较高,牛奶乳蛋白含量较低(P<0.05)。