Aluminum high pressure die casting(HPDC)technology has evolved in the past decades,enabling stronger and larger one-piece casting with significant part consolidation.It also offers a higher design freedom for more mas...Aluminum high pressure die casting(HPDC)technology has evolved in the past decades,enabling stronger and larger one-piece casting with significant part consolidation.It also offers a higher design freedom for more mass-efficient thin-walled body structures.For body structures that require excellent ductility and fracture toughness to be joined with steel sheet via self-piercing riveting(for instance,shock towers and hinge pillars,etc.),a costly T7 heat treatment comprising a solution heat treatment at elevated temperatures(450℃-500℃)followed by an over-ageing heat treatment is needed to optimize microstructure for meeting product requirement.To enable cost-efficient mass production of HPDC body structures,it is important to eliminate the expensive T7 heat treatment without sacrificing mechanical properties.Optimizing die cast alloy chemistry is a potential solution to improve fracture toughness and ductility of the HPDC components.The present study intends to tailor the Mg and Cu additions for a new Al-Si-Cr type die casting alloy(registered as A379 with The Aluminum Association,USA)to achieve the desired tensile properties without using T7 heat treatment.It was found that Cu addition should be avoided,as it is not effective in enhancing strength while degrades tensile ductility.Mg addition is very effective in improving strength and has minor impact on tensile ductility.The investigated Al-Si-Cr alloy with a nominal composition of Al-8.5wt.%Si-0.3wt.%Cr-0.2wt.%Fe shows comparable tensile properties with the T7 treated AlSi10MnMg alloy which is currently used for manufacturing shock towers and hinge pillars.展开更多
Dynamic performance on solids flow with water in deviated tubing is essential for the reliability of pump and normal operation of horizontal and directional wells.Compared with coal-water flow in vertical tubing and s...Dynamic performance on solids flow with water in deviated tubing is essential for the reliability of pump and normal operation of horizontal and directional wells.Compared with coal-water flow in vertical tubing and sand-oil flow with high production in deviated tubing,solids deposition with water shows obvious non-symmetric distributions in deviated tubing from simulations and experiments.The mathematical model of two phase flow was developed under coupling conditions of deviated tubing,low flow rate and viscosity based on the kinetic theory of granular flow and first-order discrete scheme.The results show that solid-water stratified flow in deviated tubing can be divided into two zones of suspension bed and the moving bed throughout the flow field.The solid flowing velocity with water is negative and particles slide down at the bottom of moving bed zone.The process of solids flow with water in deviated tubing will produce pressure loss and consume the kinetic energy.The thickness of deposited layer and the flowing velocity of solids flow downward with water at the moving bed zone enhance with the decreased inlet flow rate and the increased particle size,tubing inside diameter(ID)and inclination angle.Solids are easier into suspension from the upper part of moving bed zone to suspension bed zone and more solid particles flow with water towards the tubing outlet with the increase of inlet flowing velocity.The decision is made to reduce the screen width,tubing ID and inclination angle to maintain to be greater than critical deposition velocity in order to prevent solids settling.And it provides the theoretical basis and technical reserves for solid control and offers an effective approach to enhance tubing cleaning in deviated strings.展开更多
One-port surface acoustic wave resonators(SAWRs) are fabricated on semi-insulating high-quality bulk GaN and GaN film substrates, respectively. The semi-insulating GaN substrates are grown by hydride vapor phase epita...One-port surface acoustic wave resonators(SAWRs) are fabricated on semi-insulating high-quality bulk GaN and GaN film substrates, respectively. The semi-insulating GaN substrates are grown by hydride vapor phase epitaxy(HVPE)and doped with Fe. The anisotropy of Rayleigh propagation and the electromechanical coupling coefficient in Fe-doped GaN are investigated. The difference in resonance frequency between the SAWs between [1120] GaN and [1100] GaN is about 0.25% for the Rayleigh propagation mode, which is smaller than that of non-intentionally doped GaN film(~1%)reported in the literature. The electromechanical coupling coefficient of Fe-doped GaN is about 3.03%, which is higher than that of non-intentionally doped GaN film. The one-port SAWR fabricated on an 8-μm Fe-doped GaN/sapphire substrate has a quality factor of 2050, and that fabricated on Fe-doped bulk GaN has a quality factor as high as 3650. All of our results indicate that high-quality bulk GaN is a very promising material for application in SAW devices.展开更多
AIM: To screen out the differentially methylated DNA sequences between gastric primary tumor and metastatic lymph nodes, test the methylation difference of gene PTPRG between primary gastric tumor and metastatic lymph...AIM: To screen out the differentially methylated DNA sequences between gastric primary tumor and metastatic lymph nodes, test the methylation difference of gene PTPRG between primary gastric tumor and metastatic lymph nodes, and test the regulatory function of 5-aza- 2-deoxycytidine which is an agent with suppression on methylation and the level of methylation in gastric cancer cell line. METHODS: Methylated DNA sequences in genome were enriched with methylated CpG islands amplification (MCA) to undergo representational difference analysis (RDA), with MCA production of metastatic lymph nodes as tester and that of primary tumor as driver. The obtained differentially methylated fragments were cloned and sequenced to acquire the base sequence, which was analyzed with bioinformatics. With methylation-specific PCR (MSP) and RT-PCR, methylation difference of gene PTPRG was detected between primary tumor and metastatic lymph nodes in 36 cases of gastric cancer. Methylation of gene PTPRG and its regulated expression were observed in gastric cancer cell line before and after being treated with methylation-suppressive agent. RESULTS: Nineteen differentially methylated sequences were obtained and located at 5' end, exons, introns and 3' end, in which KL59 was observed to be located at 9p21 as the first exon of gene p16 and KL22 to be located at promoter region of PRPRG . KL22, as the probes, was hybridized with driver, tester and 3-round RDA products respectively with all positive signals except with the driver. Significant difference was observed in both methylation rate of gene PTPRG and PTPRG mRNA expression rate between primary tumor and metastatic lymph nodes. Demethylation of gene PTPRG, with recovered expression of PTPRG mRNA, was observed after gastric cancer cell line being treated with methylation-suppressive agent. CONCLUSION: Difference exists in DNA methylation between primary tumor and metastatic lymph nodes of gastric cancer, with MCA-RDA as one of the good analytical methods. Significant difference exists in methylation of gene PTPRG between primary tumor and metastatic lymph nodes of gastric cancer. Methylation level in gastric cancer cell line can be decreased by 5-aza-2'-deoxycytidine, which is the methylation- suppressive agent, with PTPRG expression being recovered.展开更多
Objective This paper is to explore the DNA repair mechanism of immune adaptive response (AR) induced by low dose radiation (LDR), the changes of mRNA levels and protein expressions of p53, ATM, DNA-PK catalytic su...Objective This paper is to explore the DNA repair mechanism of immune adaptive response (AR) induced by low dose radiation (LDR), the changes of mRNA levels and protein expressions of p53, ATM, DNA-PK catalytic subunit (DNA-PKcs) and PARP-1 genes in the LDR-induced AR in EL-4 cells. Methods The apoptosis and cell cycle progression of EL-4 cells were detected by flow cytometry in 12 h after the cells received the pre-exposure of 0.075 Gy X-rays (inductive dose, D 1) and the succeeding high dose irradiation (challenge dose, D2; 1.0, 1.5, and 2.0 Gy X-rays, respectively) with or without wortmannin (inhibitor of ATM and DNA-PK) and 3-aminobenzamid (inhibitor of PARP-1). And the protein expressions and mRNA levels related to these genes were detected with flow cytometry and reverse transcription-polymerase chain reaction in 12 h after irradiation with D2. Results The mRNA and protein expressions of p53 and PARP-1 in EL-4 cells in the D1 + D2 groups were much lower than those in the D2 groups, and those of PARP-1 in the 3-AB + D2 and the 3-AB + D1 + D2 groups were much lower than those in the D2 and the D1 + D2 groups. The percentage of apoptotic EL-4 cells in the 3-AB + D1 + D2 groups was much higher than that in the D1 + D2 groups, that in the G0/G1 and the G2 + M phases was much higher, and that in the S phase were much lower. Although the ATM and DNA-PKcs mRNA and protein expressions in wortmannin + D1 + D2 groups were much lower than those in the D1 + D2 groups, there were no significant changes in the apoptosis and cell cycle progression between the wortmannin + D1 + D2 and the D1 + D2 groups. Conclusion PARP-1 and p53 might play important roles in AR induced by LDR.展开更多
Doping is essential in the growth of bulk GaN substrates,which could help control the electrical properties to meet the requirements of various types of GaN-based devices.The progresses in the growth of undoped,Si-dop...Doping is essential in the growth of bulk GaN substrates,which could help control the electrical properties to meet the requirements of various types of GaN-based devices.The progresses in the growth of undoped,Si-doped,Ge-doped,Fedoped,and highly pure GaN by hydride vapor phase epitaxy(HVPE) are reviewed in this article.The growth technology and precursors of each type of doping are introduced.Besides,the influence of doping on the optical and electrical properties of GaN are presented in detail.Furthermore,the problems caused by doping,as well as the methods to solve them are also discussed.At last,highly pure GaN is briefly introduced,which points out a new way to realize high-purity semi-insulating(HPSI) GaN.展开更多
The vertical GaN-on-GaN Schottky barrier diode with boron-implanted termination was fabricated and characterized.Compared with the Schottky barrier diode(SBD)without boron-implanted termination,this SBD effectively im...The vertical GaN-on-GaN Schottky barrier diode with boron-implanted termination was fabricated and characterized.Compared with the Schottky barrier diode(SBD)without boron-implanted termination,this SBD effectively improved the breakdown voltage from 189 V to 585 V and significantly reduced the reverse leakage current by 10^5 times.In addition,a high Ion/Ioff ratio of ~10^8 was achieved by the boron-implanted technology.We used Technology Computer Aided Design(TCAD)to analyze reasons for the improved performance of the SBD with boron-implanted termination.The improved performance of diodes may be attributed to that B+could confine free carriers to suppress electron field crowding at the edge of the diode,which could improve the breakdown voltage and suppress the reverse leakage current.展开更多
Site-testing is crucial for achieving the goal of scientific research and analysis of meteorological and optical observing conditions,one of the associated basic tasks.As one of three potential sites to host the 12-me...Site-testing is crucial for achieving the goal of scientific research and analysis of meteorological and optical observing conditions,one of the associated basic tasks.As one of three potential sites to host the 12-meter Large Optical/infrared Telescope(LOT),the Muztagh-ata site,which is located on the Pamir Plateau in Xinjiang,in west China,began its site-testing task in the spring of 2017.In this paper,we firstly start with an introduction to the site and then present a statistical analysis of the ground-level meteorological properties such as air temperature,barometric pressure,relative humidity,and wind speed and direction,recorded by an automatic weather station with standard meteorological sensors for a two-year duration.We also show the monitoring results of sky brightness during this period.展开更多
BACKGROUND Osteoarthritis is a major source of pain,disability,and socioeconomic cost worldwide.Osteonecrosis is a disabling disorder that frequently occurs in the younger population aged from 20-50 years.The compound...BACKGROUND Osteoarthritis is a major source of pain,disability,and socioeconomic cost worldwide.Osteonecrosis is a disabling disorder that frequently occurs in the younger population aged from 20-50 years.The compound Eucommia bone tonic granules,a traditional Chinese medicine,can alleviate the damage of osteoarthritis and osteonecrosis.AIM To investigate the potential role of the compound Eucommia bone tonic granules(Eucommia)in the treatment of patients with osteoarthritis and osteonecrosis.METHODS One-hundred forty osteoarthritis and osteonecrosis cases admitted to our hospital from January 2013 to December 2017 were selected.Patients were divided into two groups:Eucommia-meloxicam group and meloxicam group.Clinical efficacy and the Western Ontario and McMaster Universities Arthritis Index(WOMAC)score were evaluated according to the evaluation criteria of orthopedic diseases.The levels of bone-GLA protein,interleukin-17,recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin in peripheral blood were determined.RESULTS The total effective rate in the two osteoarthritis groups was not different,but the total effective rate in the two osteonecrosis groups was significantly different.The overall efficacy of Eucommia-meloxicam group was superior to that of the meloxicam group.WOMAC showed that pain,stiffness,and dysfunction in the two groups of osteoarthritis and osteonecrosis before and after treatment were significantly different.The concentration of recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin before and after treatment in the Eucommia-meloxicam group and meloxicam group of osteoarthritis and osteonecrosis were significantly different,and the two treatment groups were significantly different from each other for osteoarthritis.CONCLUSION Our findings indicate that Eucommia can effectively enhance the curative effect of meloxicam,and the combination of Eucommia and meloxicam is superior to meloxicam alone.展开更多
An effort towards understanding the problems associated with stray light related to the Xinglong2.16-m telescope is presented to estimate the stray light performance of the telescope itself and provide a method for im...An effort towards understanding the problems associated with stray light related to the Xinglong2.16-m telescope is presented to estimate the stray light performance of the telescope itself and provide a method for improving stray light suppression.Stray light analysis for the 2.16-m telescope model,which consists of an onion shaped dome,telescope structure,equatorial mount and telescope optics,has been developed in two cases(1)pointing to 60?and(2)pointing to zenith,in both azimuth and elevation directions.The Point Source Normalized Irradiance Transmittance(PSNIT),which is generally used for assessing stray light and is uncorrelated to entrance aperture,is calculated with a series of off-axis angles.It shows that the PSNIT values are less than 10-7 when off-axis angles are larger than±20?.The dominant contributors to stray light(primary and secondary mirror,telescope structure and dome)are identified to guide performance improvement.The analyses indicate that significant benefit can be realized by adding only five vanes inside the bottom portion of the secondary baffle.In the case of pointing to zenith,the PSNIT values will decrease about 40%on average.展开更多
To evaluate performances of a back-illuminated scientific CMOS(sCMOS)camera for astronomical observations,comparison tests between Andor Marana s CMOS and Andor iKon-L 936 CCD cameras were conducted in a laboratory an...To evaluate performances of a back-illuminated scientific CMOS(sCMOS)camera for astronomical observations,comparison tests between Andor Marana s CMOS and Andor iKon-L 936 CCD cameras were conducted in a laboratory and on a telescope.The laboratory tests showed that the readout noise of the sCMOS camera is about half lower,the dark current is about 17 times higher,the dynamic range is lower in the 12-bit setting and higher in the 16-bit setting,and the linearity and bias stability are comparable relative to those of the CCD camera.In field tests,we observed the open cluster M67 with the sCMOS and CCD cameras on a 60 cm telescope.Unlike the CCD camera,the sCMOS camera has a dual-amplifier architecture.Since a 16-bit image of the sCMOS camera is composed of two 12-bit images sampled with 12-bit high gain and low gain amplifiers simultaneously,it is not real 16-bit output data.The evaluation tests indicated that the dual-amplifier architecture of the s CMOS camera leads to a decline of photometric stability by about six times around specific pixel counts.For photometry of bright objects with similar magnitudes that require high frame rates,the s CMOS camera under 12-bit setting is a good choice.Therefore,the sCMOS camera is fitted with survey observations of variable objects requiring short exposure times,mostly less than 1 s,and high frame rates.It also satisfies the requirements for an offset guiding instrument owing to its high sensitivity,high temporal resolution and high stability.展开更多
The Large Optical/infrared Telescope(LOT)is a ground-based 12 m diameter optical/infrared telescope which is proposed to be built in the western part of China in the next decade.Based on satellite remote sensing data,...The Large Optical/infrared Telescope(LOT)is a ground-based 12 m diameter optical/infrared telescope which is proposed to be built in the western part of China in the next decade.Based on satellite remote sensing data,along with geographical,logistical and political considerations,three candidate sites were chosen for ground-based astronomical performance monitoring.These sites include:Ali in Tibet,Daocheng in Sichuan and Muztagh-ata in Xinjiang.Up until now,all three sites have continuously collected data for two years.In this paper,we will introduce this site testing campaign,and present its monitoring results obtained during the period between March 2017 and March 2019.展开更多
Separation technology is an indispensable step in the preparation of freestanding GaN substrate. In this paper, a largearea freestanding GaN layer was separated from the substrate by an electrochemical liftoff process...Separation technology is an indispensable step in the preparation of freestanding GaN substrate. In this paper, a largearea freestanding GaN layer was separated from the substrate by an electrochemical liftoff process on a sandwich structure composed of an Fe-doped GaN substrate, a highly conductive Si-doped sacrificial layer and a top Fe-doped layer grown by hydride vapor phase epitaxy(HVPE). The large difference between the resistivity in the Si-doped layer and Fe-doped layer resulted in a sharp interface between the etched and unetched layer. It was found that the etching rate increased linearly with the applied voltage, while it continuously decreased with the electrochemical etching process as a result of the mass transport limitation. Flaky GaN pieces and nitrogen gas generated from the sacrificial layer by electrochemical etching were recognized as the main factors responsible for the blocking of the etching channel. Hence, a thick Si-doped layer grown by HVPE was used as the sacrificial layer to alleviate this problem. Moreover, high temperature and ultrasonic oscillation were also found to increase the etching rate. Based on the results above, we succeeded in the liftoff of ~ 1.5 inch GaN layer. This work could help reduce the cost of freestanding GaN substrate and identifies a new way for mass production.展开更多
We describe the design and construction of a new rapid 3-channel CCD photometer, dedicated to simultaneous multicolor photometric observations of rapidly variable objects. This photometer is equipped on the 1-meter te...We describe the design and construction of a new rapid 3-channel CCD photometer, dedicated to simultaneous multicolor photometric observations of rapidly variable objects. This photometer is equipped on the 1-meter telescope at the Xinglong Observatory. It allows simultaneous imaging within fields of view of 18.81′×18.8′, 18.2′×17.6′ and 9.2′×9.2′ in the Sloan Digital Sky Survey's g′, r′ and i′ bands, respectively. The results of its calibration and performance are reported.展开更多
In this article,we present a detailed analysis of the statistical properties of seeing for the Muztaghata site which is a candidate site for hosting the future Chinese Large Optical/infrared Telescope(LOT)project.The ...In this article,we present a detailed analysis of the statistical properties of seeing for the Muztaghata site which is a candidate site for hosting the future Chinese Large Optical/infrared Telescope(LOT)project.The measurements were obtained with differential image motion monitors(DIMMs)from April2017 to November 2018 at different heights during different periods.The median seeings at 11 m and6 m are very close but significantly different from that on the ground.We mainly analyzed the seeing at11 m monthly and hourly,having found that the best season for observing was from late autumn to early winter and seeing tended to improve during the night only in autumn.The analysis of the dependence on temperature inversion,wind speed and direction also was made and the best meteorological conditions for seeing are given.展开更多
Based on previous site testing and satellite cloud data,Ali,Daocheng and Muztagh-ata have been selected as candidate sites for the Large Optical/Infrared Telescope(LOT) in China.We present the data collection,processi...Based on previous site testing and satellite cloud data,Ali,Daocheng and Muztagh-ata have been selected as candidate sites for the Large Optical/Infrared Telescope(LOT) in China.We present the data collection,processing,management and quality analysis for our site testing based on using similar hardware.We analyze meteorological data,seeing,background light,cloud and precipitable water vapor data from 2017 March 10 to 2019 March 10.We also investigated the relative usefulness of our all-sky camera data in comparison to that from the meteorological TERRA satellite data based on a night-by-night comparison of the correlation and consistency between them.We find a 6% discrepancy arising from a wide range of factors.展开更多
A large ground-based optical/infrared telescope is being planned for a world-class astronomical site in China.The cloud-free night percentage is the primary meteorological consideration for evaluating candidate sites....A large ground-based optical/infrared telescope is being planned for a world-class astronomical site in China.The cloud-free night percentage is the primary meteorological consideration for evaluating candidate sites.The data from GMS and NOAA satellites and the MODIS instrument were utilized in this research,covering the period from 1996 to 2015.Our data analysis benefits from overlapping results from different independent teams as well as a uniform analysis of selected sites using GMS+NOAA data.Although significant ground-based monitoring is needed to validate these findings,we identify three different geographical regions with a high percentage of cloud-free conditions(~83%on average),which is slightly lower than at Mauna Kea and Cerro Armazones(~85%on average)and were chosen for the large international projects TMT and ELT respectively.Our study finds evidence that cloud distributions and the seasonal changes affected by the prevailing westerly winds and summer monsoons reduce the cloud cover in areas influenced by the westerlies.This is consistent with the expectations from climate change models and is suggestive that most of the identified sites will have reduced cloud cover in the future.展开更多
文摘Aluminum high pressure die casting(HPDC)technology has evolved in the past decades,enabling stronger and larger one-piece casting with significant part consolidation.It also offers a higher design freedom for more mass-efficient thin-walled body structures.For body structures that require excellent ductility and fracture toughness to be joined with steel sheet via self-piercing riveting(for instance,shock towers and hinge pillars,etc.),a costly T7 heat treatment comprising a solution heat treatment at elevated temperatures(450℃-500℃)followed by an over-ageing heat treatment is needed to optimize microstructure for meeting product requirement.To enable cost-efficient mass production of HPDC body structures,it is important to eliminate the expensive T7 heat treatment without sacrificing mechanical properties.Optimizing die cast alloy chemistry is a potential solution to improve fracture toughness and ductility of the HPDC components.The present study intends to tailor the Mg and Cu additions for a new Al-Si-Cr type die casting alloy(registered as A379 with The Aluminum Association,USA)to achieve the desired tensile properties without using T7 heat treatment.It was found that Cu addition should be avoided,as it is not effective in enhancing strength while degrades tensile ductility.Mg addition is very effective in improving strength and has minor impact on tensile ductility.The investigated Al-Si-Cr alloy with a nominal composition of Al-8.5wt.%Si-0.3wt.%Cr-0.2wt.%Fe shows comparable tensile properties with the T7 treated AlSi10MnMg alloy which is currently used for manufacturing shock towers and hinge pillars.
基金funded by National Natural Science Foundation of China(Grant No.52074161)National Science and Technology Major Project of China(Grant No.2016ZX05065-001)+2 种基金Taishan Scholar Project of Shandong Province(Grant No.tsqn202211177)Shandong Provincial Plan for Introduction and Cultivation of Young Pioneers in Colleges and Universities(Grant No.2021-QingChuang-30613019)Natural Science Foundation of Shandong Province(Grant No.ZR2022ME173).
文摘Dynamic performance on solids flow with water in deviated tubing is essential for the reliability of pump and normal operation of horizontal and directional wells.Compared with coal-water flow in vertical tubing and sand-oil flow with high production in deviated tubing,solids deposition with water shows obvious non-symmetric distributions in deviated tubing from simulations and experiments.The mathematical model of two phase flow was developed under coupling conditions of deviated tubing,low flow rate and viscosity based on the kinetic theory of granular flow and first-order discrete scheme.The results show that solid-water stratified flow in deviated tubing can be divided into two zones of suspension bed and the moving bed throughout the flow field.The solid flowing velocity with water is negative and particles slide down at the bottom of moving bed zone.The process of solids flow with water in deviated tubing will produce pressure loss and consume the kinetic energy.The thickness of deposited layer and the flowing velocity of solids flow downward with water at the moving bed zone enhance with the decreased inlet flow rate and the increased particle size,tubing inside diameter(ID)and inclination angle.Solids are easier into suspension from the upper part of moving bed zone to suspension bed zone and more solid particles flow with water towards the tubing outlet with the increase of inlet flowing velocity.The decision is made to reduce the screen width,tubing ID and inclination angle to maintain to be greater than critical deposition velocity in order to prevent solids settling.And it provides the theoretical basis and technical reserves for solid control and offers an effective approach to enhance tubing cleaning in deviated strings.
基金Project supported by the National Key Research and Development Program of China(Grant No.2017YFB0403002)the National Science Fund for Distinguished Young Scholars,China(Grant No.Y3CHC11001)the National Natural Science Foundation of China(Grant No.11604368)
文摘One-port surface acoustic wave resonators(SAWRs) are fabricated on semi-insulating high-quality bulk GaN and GaN film substrates, respectively. The semi-insulating GaN substrates are grown by hydride vapor phase epitaxy(HVPE)and doped with Fe. The anisotropy of Rayleigh propagation and the electromechanical coupling coefficient in Fe-doped GaN are investigated. The difference in resonance frequency between the SAWs between [1120] GaN and [1100] GaN is about 0.25% for the Rayleigh propagation mode, which is smaller than that of non-intentionally doped GaN film(~1%)reported in the literature. The electromechanical coupling coefficient of Fe-doped GaN is about 3.03%, which is higher than that of non-intentionally doped GaN film. The one-port SAWR fabricated on an 8-μm Fe-doped GaN/sapphire substrate has a quality factor of 2050, and that fabricated on Fe-doped bulk GaN has a quality factor as high as 3650. All of our results indicate that high-quality bulk GaN is a very promising material for application in SAW devices.
基金the National Natural Science Foundation of China, No.30271477 and No.30572162 the Special Scientific Research Foundation for Doctors, State Education Ministry, No.20050159001
文摘AIM: To screen out the differentially methylated DNA sequences between gastric primary tumor and metastatic lymph nodes, test the methylation difference of gene PTPRG between primary gastric tumor and metastatic lymph nodes, and test the regulatory function of 5-aza- 2-deoxycytidine which is an agent with suppression on methylation and the level of methylation in gastric cancer cell line. METHODS: Methylated DNA sequences in genome were enriched with methylated CpG islands amplification (MCA) to undergo representational difference analysis (RDA), with MCA production of metastatic lymph nodes as tester and that of primary tumor as driver. The obtained differentially methylated fragments were cloned and sequenced to acquire the base sequence, which was analyzed with bioinformatics. With methylation-specific PCR (MSP) and RT-PCR, methylation difference of gene PTPRG was detected between primary tumor and metastatic lymph nodes in 36 cases of gastric cancer. Methylation of gene PTPRG and its regulated expression were observed in gastric cancer cell line before and after being treated with methylation-suppressive agent. RESULTS: Nineteen differentially methylated sequences were obtained and located at 5' end, exons, introns and 3' end, in which KL59 was observed to be located at 9p21 as the first exon of gene p16 and KL22 to be located at promoter region of PRPRG . KL22, as the probes, was hybridized with driver, tester and 3-round RDA products respectively with all positive signals except with the driver. Significant difference was observed in both methylation rate of gene PTPRG and PTPRG mRNA expression rate between primary tumor and metastatic lymph nodes. Demethylation of gene PTPRG, with recovered expression of PTPRG mRNA, was observed after gastric cancer cell line being treated with methylation-suppressive agent. CONCLUSION: Difference exists in DNA methylation between primary tumor and metastatic lymph nodes of gastric cancer, with MCA-RDA as one of the good analytical methods. Significant difference exists in methylation of gene PTPRG between primary tumor and metastatic lymph nodes of gastric cancer. Methylation level in gastric cancer cell line can be decreased by 5-aza-2'-deoxycytidine, which is the methylation- suppressive agent, with PTPRG expression being recovered.
基金supported by the National Natural Science Foundation of China(30570546 and 30870747)the Natural Science Foundation of Jilin Province (20090458 and 201015183)+1 种基金the Young Teachers Innovative Foundation of Jilin University(421010043430)the Young Scholars Research Foundation Program of China-Japan Union Hospital(2009)
文摘Objective This paper is to explore the DNA repair mechanism of immune adaptive response (AR) induced by low dose radiation (LDR), the changes of mRNA levels and protein expressions of p53, ATM, DNA-PK catalytic subunit (DNA-PKcs) and PARP-1 genes in the LDR-induced AR in EL-4 cells. Methods The apoptosis and cell cycle progression of EL-4 cells were detected by flow cytometry in 12 h after the cells received the pre-exposure of 0.075 Gy X-rays (inductive dose, D 1) and the succeeding high dose irradiation (challenge dose, D2; 1.0, 1.5, and 2.0 Gy X-rays, respectively) with or without wortmannin (inhibitor of ATM and DNA-PK) and 3-aminobenzamid (inhibitor of PARP-1). And the protein expressions and mRNA levels related to these genes were detected with flow cytometry and reverse transcription-polymerase chain reaction in 12 h after irradiation with D2. Results The mRNA and protein expressions of p53 and PARP-1 in EL-4 cells in the D1 + D2 groups were much lower than those in the D2 groups, and those of PARP-1 in the 3-AB + D2 and the 3-AB + D1 + D2 groups were much lower than those in the D2 and the D1 + D2 groups. The percentage of apoptotic EL-4 cells in the 3-AB + D1 + D2 groups was much higher than that in the D1 + D2 groups, that in the G0/G1 and the G2 + M phases was much higher, and that in the S phase were much lower. Although the ATM and DNA-PKcs mRNA and protein expressions in wortmannin + D1 + D2 groups were much lower than those in the D1 + D2 groups, there were no significant changes in the apoptosis and cell cycle progression between the wortmannin + D1 + D2 and the D1 + D2 groups. Conclusion PARP-1 and p53 might play important roles in AR induced by LDR.
基金Project supported by the National Key Research and Development Program of China(Grant Nos.2017YFB0404100 and 2016YFA0201101)the National Natural Science Foundation of China(Grant Nos.61574164,61704187,and 61604170)+2 种基金the Key Research Program of the Frontier Science of the Chinese Academy of Sciences(Grant No.QYZDB-SSW-SLH042)the State Key Program of the National Natural Science Foundation of China(Grant Nos.61734008and 11435010)the National Key Scientific Instrument and Equipment Development Project,China(Grant No.11327804)。
文摘Doping is essential in the growth of bulk GaN substrates,which could help control the electrical properties to meet the requirements of various types of GaN-based devices.The progresses in the growth of undoped,Si-doped,Ge-doped,Fedoped,and highly pure GaN by hydride vapor phase epitaxy(HVPE) are reviewed in this article.The growth technology and precursors of each type of doping are introduced.Besides,the influence of doping on the optical and electrical properties of GaN are presented in detail.Furthermore,the problems caused by doping,as well as the methods to solve them are also discussed.At last,highly pure GaN is briefly introduced,which points out a new way to realize high-purity semi-insulating(HPSI) GaN.
基金Project supported by the National Key R&D Program of China(Grant No.2017YFB0404100)Science and Technology Planning Project of Guangdong Province,China(Grant No.2017B010112001)。
文摘The vertical GaN-on-GaN Schottky barrier diode with boron-implanted termination was fabricated and characterized.Compared with the Schottky barrier diode(SBD)without boron-implanted termination,this SBD effectively improved the breakdown voltage from 189 V to 585 V and significantly reduced the reverse leakage current by 10^5 times.In addition,a high Ion/Ioff ratio of ~10^8 was achieved by the boron-implanted technology.We used Technology Computer Aided Design(TCAD)to analyze reasons for the improved performance of the SBD with boron-implanted termination.The improved performance of diodes may be attributed to that B+could confine free carriers to suppress electron field crowding at the edge of the diode,which could improve the breakdown voltage and suppress the reverse leakage current.
基金supported by the National Natural Science Foundation of China(Grant Nos.11873081 and 11603065)the Operation,Maintenance and Upgrading Fund for Astronomical Telescopes and Facility Instruments,budgeted from the Ministry of Finance of China and administered by the Chinese Academy of Sciences。
文摘Site-testing is crucial for achieving the goal of scientific research and analysis of meteorological and optical observing conditions,one of the associated basic tasks.As one of three potential sites to host the 12-meter Large Optical/infrared Telescope(LOT),the Muztagh-ata site,which is located on the Pamir Plateau in Xinjiang,in west China,began its site-testing task in the spring of 2017.In this paper,we firstly start with an introduction to the site and then present a statistical analysis of the ground-level meteorological properties such as air temperature,barometric pressure,relative humidity,and wind speed and direction,recorded by an automatic weather station with standard meteorological sensors for a two-year duration.We also show the monitoring results of sky brightness during this period.
文摘BACKGROUND Osteoarthritis is a major source of pain,disability,and socioeconomic cost worldwide.Osteonecrosis is a disabling disorder that frequently occurs in the younger population aged from 20-50 years.The compound Eucommia bone tonic granules,a traditional Chinese medicine,can alleviate the damage of osteoarthritis and osteonecrosis.AIM To investigate the potential role of the compound Eucommia bone tonic granules(Eucommia)in the treatment of patients with osteoarthritis and osteonecrosis.METHODS One-hundred forty osteoarthritis and osteonecrosis cases admitted to our hospital from January 2013 to December 2017 were selected.Patients were divided into two groups:Eucommia-meloxicam group and meloxicam group.Clinical efficacy and the Western Ontario and McMaster Universities Arthritis Index(WOMAC)score were evaluated according to the evaluation criteria of orthopedic diseases.The levels of bone-GLA protein,interleukin-17,recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin in peripheral blood were determined.RESULTS The total effective rate in the two osteoarthritis groups was not different,but the total effective rate in the two osteonecrosis groups was significantly different.The overall efficacy of Eucommia-meloxicam group was superior to that of the meloxicam group.WOMAC showed that pain,stiffness,and dysfunction in the two groups of osteoarthritis and osteonecrosis before and after treatment were significantly different.The concentration of recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin before and after treatment in the Eucommia-meloxicam group and meloxicam group of osteoarthritis and osteonecrosis were significantly different,and the two treatment groups were significantly different from each other for osteoarthritis.CONCLUSION Our findings indicate that Eucommia can effectively enhance the curative effect of meloxicam,and the combination of Eucommia and meloxicam is superior to meloxicam alone.
基金supported by the National Natural Science Foundation of China(Grant No.11703043)Joint Research Fund in Astronomy,National Natural Science Foundation of China(No.U1831209).
文摘An effort towards understanding the problems associated with stray light related to the Xinglong2.16-m telescope is presented to estimate the stray light performance of the telescope itself and provide a method for improving stray light suppression.Stray light analysis for the 2.16-m telescope model,which consists of an onion shaped dome,telescope structure,equatorial mount and telescope optics,has been developed in two cases(1)pointing to 60?and(2)pointing to zenith,in both azimuth and elevation directions.The Point Source Normalized Irradiance Transmittance(PSNIT),which is generally used for assessing stray light and is uncorrelated to entrance aperture,is calculated with a series of off-axis angles.It shows that the PSNIT values are less than 10-7 when off-axis angles are larger than±20?.The dominant contributors to stray light(primary and secondary mirror,telescope structure and dome)are identified to guide performance improvement.The analyses indicate that significant benefit can be realized by adding only five vanes inside the bottom portion of the secondary baffle.In the case of pointing to zenith,the PSNIT values will decrease about 40%on average.
基金supported by the National Natural Science Foundation of China(Grant Nos.U1831209,11803017 and 11903053)。
文摘To evaluate performances of a back-illuminated scientific CMOS(sCMOS)camera for astronomical observations,comparison tests between Andor Marana s CMOS and Andor iKon-L 936 CCD cameras were conducted in a laboratory and on a telescope.The laboratory tests showed that the readout noise of the sCMOS camera is about half lower,the dark current is about 17 times higher,the dynamic range is lower in the 12-bit setting and higher in the 16-bit setting,and the linearity and bias stability are comparable relative to those of the CCD camera.In field tests,we observed the open cluster M67 with the sCMOS and CCD cameras on a 60 cm telescope.Unlike the CCD camera,the sCMOS camera has a dual-amplifier architecture.Since a 16-bit image of the sCMOS camera is composed of two 12-bit images sampled with 12-bit high gain and low gain amplifiers simultaneously,it is not real 16-bit output data.The evaluation tests indicated that the dual-amplifier architecture of the s CMOS camera leads to a decline of photometric stability by about six times around specific pixel counts.For photometry of bright objects with similar magnitudes that require high frame rates,the s CMOS camera under 12-bit setting is a good choice.Therefore,the sCMOS camera is fitted with survey observations of variable objects requiring short exposure times,mostly less than 1 s,and high frame rates.It also satisfies the requirements for an offset guiding instrument owing to its high sensitivity,high temporal resolution and high stability.
基金supported by the Operation,Maintenance and Upgrading Fund for Astronomical Telescopes and Facility Instruments,budgeted from the Ministry of Finance of China(MOF)and administered by the Chinese Academy of Sciences(CAS)supported by the National Natural Science Foundation of China(Grant No.11873081)。
文摘The Large Optical/infrared Telescope(LOT)is a ground-based 12 m diameter optical/infrared telescope which is proposed to be built in the western part of China in the next decade.Based on satellite remote sensing data,along with geographical,logistical and political considerations,three candidate sites were chosen for ground-based astronomical performance monitoring.These sites include:Ali in Tibet,Daocheng in Sichuan and Muztagh-ata in Xinjiang.Up until now,all three sites have continuously collected data for two years.In this paper,we will introduce this site testing campaign,and present its monitoring results obtained during the period between March 2017 and March 2019.
基金supported by the National Key R&D Program of China (Grant Nos. 2017YFB0404100 and 2017YFB0403000)the National Natural Science Foundation of China (Grant No. 61704187)the Key Research Program of the Frontier Science of the Chinese Academy of Sciences (Grant No. QYZDB-SSWSLH042)。
文摘Separation technology is an indispensable step in the preparation of freestanding GaN substrate. In this paper, a largearea freestanding GaN layer was separated from the substrate by an electrochemical liftoff process on a sandwich structure composed of an Fe-doped GaN substrate, a highly conductive Si-doped sacrificial layer and a top Fe-doped layer grown by hydride vapor phase epitaxy(HVPE). The large difference between the resistivity in the Si-doped layer and Fe-doped layer resulted in a sharp interface between the etched and unetched layer. It was found that the etching rate increased linearly with the applied voltage, while it continuously decreased with the electrochemical etching process as a result of the mass transport limitation. Flaky GaN pieces and nitrogen gas generated from the sacrificial layer by electrochemical etching were recognized as the main factors responsible for the blocking of the etching channel. Hence, a thick Si-doped layer grown by HVPE was used as the sacrificial layer to alleviate this problem. Moreover, high temperature and ultrasonic oscillation were also found to increase the etching rate. Based on the results above, we succeeded in the liftoff of ~ 1.5 inch GaN layer. This work could help reduce the cost of freestanding GaN substrate and identifies a new way for mass production.
文摘We describe the design and construction of a new rapid 3-channel CCD photometer, dedicated to simultaneous multicolor photometric observations of rapidly variable objects. This photometer is equipped on the 1-meter telescope at the Xinglong Observatory. It allows simultaneous imaging within fields of view of 18.81′×18.8′, 18.2′×17.6′ and 9.2′×9.2′ in the Sloan Digital Sky Survey's g′, r′ and i′ bands, respectively. The results of its calibration and performance are reported.
基金supported by theNational Nature Science Foundation of China(Grant Nos.11873081 and 11603065)the Operation,Maintenance and Upgrading Fund for Astronomical Telescopes and Facility Instruments,budgeted from the Ministry of Finance of China(MOF)and administered by the Chinese Academy of Sciences。
文摘In this article,we present a detailed analysis of the statistical properties of seeing for the Muztaghata site which is a candidate site for hosting the future Chinese Large Optical/infrared Telescope(LOT)project.The measurements were obtained with differential image motion monitors(DIMMs)from April2017 to November 2018 at different heights during different periods.The median seeings at 11 m and6 m are very close but significantly different from that on the ground.We mainly analyzed the seeing at11 m monthly and hourly,having found that the best season for observing was from late autumn to early winter and seeing tended to improve during the night only in autumn.The analysis of the dependence on temperature inversion,wind speed and direction also was made and the best meteorological conditions for seeing are given.
基金partly supported by the Operation,Maintenance and Upgrading Fund for Astronomical Telescopes and Facility Instruments,budgeted from the Ministry of Finance of China (MOF) and administered by the Chinese Academy of Sciences (CAS)supported by the National NaturalScience Foundation of China (Grant Nos.11573054,11703065,11603044 and 11873081)HRAJ acknowledges support from a CAS PIFI and UK STFC grant ST/R006598/1。
文摘Based on previous site testing and satellite cloud data,Ali,Daocheng and Muztagh-ata have been selected as candidate sites for the Large Optical/Infrared Telescope(LOT) in China.We present the data collection,processing,management and quality analysis for our site testing based on using similar hardware.We analyze meteorological data,seeing,background light,cloud and precipitable water vapor data from 2017 March 10 to 2019 March 10.We also investigated the relative usefulness of our all-sky camera data in comparison to that from the meteorological TERRA satellite data based on a night-by-night comparison of the correlation and consistency between them.We find a 6% discrepancy arising from a wide range of factors.
基金partly supported by the Operation,Maintenance and Upgrading Fund for Astronomical Telescopes and Facility Instruments,budgeted from the Ministry of Finance of China(MOF)and administered by the Chinese Academy of Sciences(CAS)supported by the National Natural Science Foundation of China(Grant Nos.11573054,11703065,11603044 and 11873081)+1 种基金support from a CAS PIFIUK STFC grant ST/R006598/1。
文摘A large ground-based optical/infrared telescope is being planned for a world-class astronomical site in China.The cloud-free night percentage is the primary meteorological consideration for evaluating candidate sites.The data from GMS and NOAA satellites and the MODIS instrument were utilized in this research,covering the period from 1996 to 2015.Our data analysis benefits from overlapping results from different independent teams as well as a uniform analysis of selected sites using GMS+NOAA data.Although significant ground-based monitoring is needed to validate these findings,we identify three different geographical regions with a high percentage of cloud-free conditions(~83%on average),which is slightly lower than at Mauna Kea and Cerro Armazones(~85%on average)and were chosen for the large international projects TMT and ELT respectively.Our study finds evidence that cloud distributions and the seasonal changes affected by the prevailing westerly winds and summer monsoons reduce the cloud cover in areas influenced by the westerlies.This is consistent with the expectations from climate change models and is suggestive that most of the identified sites will have reduced cloud cover in the future.