Succinic acid is considered as an important platform chemical.Succinic acid fermentation with Actinobacillus succinogenes strain BE-1 was optimized by central composite design(CCD)using a response surface methodology(...Succinic acid is considered as an important platform chemical.Succinic acid fermentation with Actinobacillus succinogenes strain BE-1 was optimized by central composite design(CCD)using a response surface methodology(RSM).The optimized production of succinic acid was predicted and the interactive effects between glucose,yeast extract,and magnesium carbonate were investigated.As a result,a model for predicting the concentration of succinic acid production was developed.The accuracy of the model was confirmed by the analysis of variance(ANOVA),and the validity was further proved by verification experiments showing that percentage errors between actual and predicted values varied from 3.02%to 6.38%.In addition,it was observed that the interactive effect between yeast extract and magnesium carbonate was statistically significant.In conclusion,RSM is an effective and useful method for optimizing the medium components and investigating the interactive effects,and can provide valuable information for succinic acid scale-up fermentation using A.succinogenes strain BE-1.展开更多
A simple and efficient method of enhancing biomass saccharification by microwave-assisted pretreatment with dimethyl sulfoxide/1-allyl-3-methylimidazolium chloride is proposed. Softwood（pine wood（PW））, hardwoods（...A simple and efficient method of enhancing biomass saccharification by microwave-assisted pretreatment with dimethyl sulfoxide/1-allyl-3-methylimidazolium chloride is proposed. Softwood（pine wood（PW））, hardwoods（poplar wood, catalpa bungi, and Chinese parasol）, and agricultural wastes（rice straw, wheat straw, and corn stover（CS）） were exploited. Results showed that the best pretreatment effect was in PW with 54.3% and 31.7% dissolution and extraction ratios, respectively. The crystal form of cellulose in PW extract transformed from I to II, and the contended cellulose ratio and glucose conversion ratio reached 85.1% and 85.4%, respectively. CS after steam explosion achieved a similar pretreating effect as PW, with its cellulose hydrolysis ratio reaching as high as 91.5% after IL pretreatment.展开更多
基金supported by the National Major Special Project on New Varieties Cultivation for Transgenic Organisms(No.2009ZX08009-130B)the National Natural Science Foundation of China(No.50874112)the National High-Tech R&D Program(863)of China(No.2009AA06Z320)
文摘Succinic acid is considered as an important platform chemical.Succinic acid fermentation with Actinobacillus succinogenes strain BE-1 was optimized by central composite design(CCD)using a response surface methodology(RSM).The optimized production of succinic acid was predicted and the interactive effects between glucose,yeast extract,and magnesium carbonate were investigated.As a result,a model for predicting the concentration of succinic acid production was developed.The accuracy of the model was confirmed by the analysis of variance(ANOVA),and the validity was further proved by verification experiments showing that percentage errors between actual and predicted values varied from 3.02%to 6.38%.In addition,it was observed that the interactive effect between yeast extract and magnesium carbonate was statistically significant.In conclusion,RSM is an effective and useful method for optimizing the medium components and investigating the interactive effects,and can provide valuable information for succinic acid scale-up fermentation using A.succinogenes strain BE-1.
基金supported by the National Natural Science Foundation of China(No.21006118)the National High Technology Research and Development Program of China(863Project,Nos.2012AA101807 and 2012AA022301)
文摘A simple and efficient method of enhancing biomass saccharification by microwave-assisted pretreatment with dimethyl sulfoxide/1-allyl-3-methylimidazolium chloride is proposed. Softwood（pine wood（PW））, hardwoods（poplar wood, catalpa bungi, and Chinese parasol）, and agricultural wastes（rice straw, wheat straw, and corn stover（CS）） were exploited. Results showed that the best pretreatment effect was in PW with 54.3% and 31.7% dissolution and extraction ratios, respectively. The crystal form of cellulose in PW extract transformed from I to II, and the contended cellulose ratio and glucose conversion ratio reached 85.1% and 85.4%, respectively. CS after steam explosion achieved a similar pretreating effect as PW, with its cellulose hydrolysis ratio reaching as high as 91.5% after IL pretreatment.
