Photosynthesis is involved in the essential process of transforming light energy into chemical energy.Although the interaction between photosynthesis and the circadian clock has been confirmed,the mechanism of how lig...Photosynthesis is involved in the essential process of transforming light energy into chemical energy.Although the interaction between photosynthesis and the circadian clock has been confirmed,the mechanism of how light intensity affects photosynthesis through the circadian clock remains unclear.Here,we propose a first computational model for circadian-clock-controlled photosynthesis,which consists of the light-sensitive protein P,the core oscillator,photosynthetic genes,and parameters involved in the process of photosynthesis.The model parameters were determined by minimizing the cost function(δ=8.56),which is defined by the errors of expression levels,periods,and phases of the clock genes(CCA1,PRR9,TOC1,ELF4,GI,and RVE8).The model recapitulates the expression pattern of the core oscillator under moderate light intensity(100μmol m^(-2) s^(-1)).Further simulation validated the dynamic behaviors of the circadian clock and photosynthetic outputs under low(62.5μmol m^(-2) s^(-1))and normal(187.5μmol m^(-2) s^(-1))intensities.When exposed to low light intensity,the peak times of clock and photosynthetic genes were shifted backward by 1–2 hours,the period was elongated by approximately the same length,and the photosynthetic parameters attained low values and showed delayed peak times,which confirmed our model predictions.Our study reveals a potential mechanism underlying the circadian regulation of photosynthesis by the clock under different light intensities in tomato.展开更多
Water dropwort(Liyang Baiqin,Oenanthe javanica(BI.)DC.)is an aquatic perennial plant from the Apiaceae family with abundant protein,dietary fiber,vitamins,and minerals.It usually grows in wet soils and can even grow i...Water dropwort(Liyang Baiqin,Oenanthe javanica(BI.)DC.)is an aquatic perennial plant from the Apiaceae family with abundant protein,dietary fiber,vitamins,and minerals.It usually grows in wet soils and can even grow in water.Here,whole-genome sequencing of O.javanica via HiSeq 2000 sequencing technology was reported for the first time.The genome size was 1.28 Gb,including 42,270 genes,of which 93.92%could be functionally annotated.An online database of the whole-genome sequences of water dropwort,Water dropwortDB,was established to share the results and facilitate further research on O.javanica(database homepage:http://apiaceae.njau.edu.cn/waterdropwortdb).Water dropwortDB offers whole-genome and transcriptome sequences and a Basic Local Alignment Search Tool.Comparative analysis with other species showed that the evolutionary relationship between O.javanica and Daucus carota was the closest.Twenty-five gene families of O.javanica were found to be expanded,and some genetic factors(such as genes and miRNAs)related to phenotypic and anatomic differentiation in O.javanica under different water conditions were further investigated.Two miRNA and target gene pairs(miR408 and Oja15472,miR171 and Oja47040)were remarkably regulated by water stress.The obtained reference genome of O.javanica provides important information for future work,thus making in-depth genetic breeding and gene editing possible.The present study also provides a foundation for the understanding of the O.javanica response to water stress,including morphological,anatomical,and genetic differentiation.展开更多
Forest structure and succession in Wolong Nature Reserve is influenced by the understory dwarf bamboo population. However, less is known about how the forest succession affects the dwarf bamboo population. To examine ...Forest structure and succession in Wolong Nature Reserve is influenced by the understory dwarf bamboo population. However, less is known about how the forest succession affects the dwarf bamboo population. To examine the bamboo ramet population growth of Fargesla nitida （Mltford） Keng f. et Yi and to determine how ramet population structure varies along the succession of coniferous forest, we sampled ramet populations of F. nitida from the following three successional stages： （i） a deciduous broad-leaved （BL） stand; （ii） a mixed broad-leaved coniferous （MI） stand; and （ill） a coniferous （CF） stand. We investigated the population structure, biomass allocation, and morphological characteristics of the bamboo ramet among the three stand types. Clonal ramets, constituting the bamboo population, tended to become short and small with succession. The ramet changed towards having a greater mass investment in leaves, branches and underground roots and rhizomes rather than in the culm. With respect to leaf traits, individual leaf mass and area in the BL stand were markedly bigger than those In both the MI and CF stands, except for no significant difference in specific leaf area. The age distribution showed that the bamboo population approached an older age with succession. The results demonstrate that the ramet population structure of F. nitida is unstable and its growth performance is inhibited by succession.展开更多
基金This work was partially supported by the National Natural Science Foundation of China(11171155,11871268)the National Natural Science Foundation of Jiangsu Province,China(BK20171370,BK20221009)+2 种基金the Jiangsu Agricultural Science and Technology Innovation Fund[CX(21)3025]the China Postdoctoral Science Foundation(2021 M701742)the Priority Academic Program Development of Jiangsu Higher Education Institution Project(PAPD).
文摘Photosynthesis is involved in the essential process of transforming light energy into chemical energy.Although the interaction between photosynthesis and the circadian clock has been confirmed,the mechanism of how light intensity affects photosynthesis through the circadian clock remains unclear.Here,we propose a first computational model for circadian-clock-controlled photosynthesis,which consists of the light-sensitive protein P,the core oscillator,photosynthetic genes,and parameters involved in the process of photosynthesis.The model parameters were determined by minimizing the cost function(δ=8.56),which is defined by the errors of expression levels,periods,and phases of the clock genes(CCA1,PRR9,TOC1,ELF4,GI,and RVE8).The model recapitulates the expression pattern of the core oscillator under moderate light intensity(100μmol m^(-2) s^(-1)).Further simulation validated the dynamic behaviors of the circadian clock and photosynthetic outputs under low(62.5μmol m^(-2) s^(-1))and normal(187.5μmol m^(-2) s^(-1))intensities.When exposed to low light intensity,the peak times of clock and photosynthetic genes were shifted backward by 1–2 hours,the period was elongated by approximately the same length,and the photosynthetic parameters attained low values and showed delayed peak times,which confirmed our model predictions.Our study reveals a potential mechanism underlying the circadian regulation of photosynthesis by the clock under different light intensities in tomato.
基金This research was supported by Jiangsu Agricultural Science and Technology Innovation Fund(CX(18)2007)New Century Excellent Talents in University(NCET-11-0670)+1 种基金National Natural Science Foundation of China(31272175)Priority Academic Program Development of Jiangsu Higher Education Institutions Project(PAPD).
文摘Water dropwort(Liyang Baiqin,Oenanthe javanica(BI.)DC.)is an aquatic perennial plant from the Apiaceae family with abundant protein,dietary fiber,vitamins,and minerals.It usually grows in wet soils and can even grow in water.Here,whole-genome sequencing of O.javanica via HiSeq 2000 sequencing technology was reported for the first time.The genome size was 1.28 Gb,including 42,270 genes,of which 93.92%could be functionally annotated.An online database of the whole-genome sequences of water dropwort,Water dropwortDB,was established to share the results and facilitate further research on O.javanica(database homepage:http://apiaceae.njau.edu.cn/waterdropwortdb).Water dropwortDB offers whole-genome and transcriptome sequences and a Basic Local Alignment Search Tool.Comparative analysis with other species showed that the evolutionary relationship between O.javanica and Daucus carota was the closest.Twenty-five gene families of O.javanica were found to be expanded,and some genetic factors(such as genes and miRNAs)related to phenotypic and anatomic differentiation in O.javanica under different water conditions were further investigated.Two miRNA and target gene pairs(miR408 and Oja15472,miR171 and Oja47040)were remarkably regulated by water stress.The obtained reference genome of O.javanica provides important information for future work,thus making in-depth genetic breeding and gene editing possible.The present study also provides a foundation for the understanding of the O.javanica response to water stress,including morphological,anatomical,and genetic differentiation.
基金Supported by the State Key Basic Research and Development Plan of China （2002CB111505） and the National Nature Science Foundation of China （30300047, 30500388）. Acknowledgements The authors thank Hans Cornelissen （Vrije Universiteit, Amsterdam, The Netherlands） and Ken Chart （University of the Sunshine Coast, 0ueensland, Australia） for their valuable comments on our earlier manuscript. Thanks are also due to the students from Nanjing Forestry University and Sichuan Agricultural University and the staff of Wolong Subalpine Dark Coniferous Forest Ecosystem Ecological Station, Sichuan Academy of Forestry, who helped with the field work.
文摘Forest structure and succession in Wolong Nature Reserve is influenced by the understory dwarf bamboo population. However, less is known about how the forest succession affects the dwarf bamboo population. To examine the bamboo ramet population growth of Fargesla nitida （Mltford） Keng f. et Yi and to determine how ramet population structure varies along the succession of coniferous forest, we sampled ramet populations of F. nitida from the following three successional stages： （i） a deciduous broad-leaved （BL） stand; （ii） a mixed broad-leaved coniferous （MI） stand; and （ill） a coniferous （CF） stand. We investigated the population structure, biomass allocation, and morphological characteristics of the bamboo ramet among the three stand types. Clonal ramets, constituting the bamboo population, tended to become short and small with succession. The ramet changed towards having a greater mass investment in leaves, branches and underground roots and rhizomes rather than in the culm. With respect to leaf traits, individual leaf mass and area in the BL stand were markedly bigger than those In both the MI and CF stands, except for no significant difference in specific leaf area. The age distribution showed that the bamboo population approached an older age with succession. The results demonstrate that the ramet population structure of F. nitida is unstable and its growth performance is inhibited by succession.
