Dopamine and cyclic adenosine monophosphate-regulated phosphoprotein with an apparent Mr of 32000 promotes colorectal cancer growth

BACKGROUND Dopamine and cyclic adenosine monophosphate(cAMP)-regulated phosphop-rotein with an apparent Mr of 32000(DARPP-32)is a protein that is involved in regulating dopamine and cAMP signaling pathways in the brain.However,recent studies have shown that DARPP-32 is also expressed in other tissues,including colorectal cancer(CRC),where its function is not well understood.AIM To explore the effect of DARPP-32 on CRC progression.METHODS The expression levels of DARPP-32 were assessed in CRC tissues using both quantitative polymerase chain reaction and immunohistochemistry assays.The proliferative capacity of CRC cell lines was evaluated with Cell Counting Kit-8 and 5-ethynyl-2’-deoxyuridine assays,while apoptosis was measured by flow cytometry.The migratory and invasive potential of CRC cell lines were deter-mined using wound healing and transwell chamber assays.In vivo studies involved monitoring the growth rate of xenograft tumors.Finally,the underlying molecular mechanism of DARPP-32 was investigated through RNA-sequencing and western blot analyses.RESULTS DARPP-32 was frequently upregulated in CRC and associated with abnormal clinicopathological features in CRC.Overexpression of DARPP-32 was shown to promote cancer cell proliferation,migration,and invasion and reduce apoptosis.DARPP-32 knockdown resulted in the opposite functional effects.Mechanistically,DARPP-32 may regulate the phosphoinositide 3-kinase(PI3K)/AKT signaling pathway in order to carry out its biological function.CONCLUSION DARPP-32 promotes CRC progression via the PI3K/AKT signaling pathway.

Insight into the change in carbon structure and thermodynamics during anthracite transformation into graphite

The thermodynamic and kinetic mechanisms of Taixi anthracite during its graphitization process were explored.To understand the variation trends of carbon arrangement order,microcrystal size,and graphitization degree against temperature during the graphitization process,a series of experiments were performed using Raman spectroscopy and X-ray diffraction(XRD).Subsequently,the influencing factors of the dominant reaction at different temperatures were analyzed using thermodynamics and kinetics.The results showed that the graphitization process of Taixi anthracite can be divided into three stages from the perspective of reaction thermodynamics and kinetics.Temperature played a crucial role in the formation and growth of a graphitic structure.Meanwhile,multivariate mechanisms coexisted in the graphitization process.At ultrahigh temperatures,the defects of synthetic graphite could not be completely eliminated and perfect graphite crystals could not be produced.At low temperatures,the reaction is mainly controlled by dynamics,while at high temperatures,thermodynamics dominates the direction of the reaction.

Microstructure and mechanical properties of Cu/Al joints brazed using(Cu,Ni,Zr,Er)-modified Al−Si filler alloys

To design a promising Al−Si filler alloy with a relatively low melting-point,good strength and plasticity for the Cu/Al joint,the Cu,Ni,Zr and Er elements were innovatively added to modify the traditional Al−Si eutectic filler.The microstructure and mechanical properties of filler alloys and Cu/Al joints were investigated.The result indicated that the Al−Si−Ni−Cu filler alloys mainly consisted of Al(s,s),Al_(2)(Cu,Ni)and Si(s,s).The Al−10Si−2Ni−6Cu filler alloy exhibited relatively low solidus(521℃)and liquidus(577℃)temperature,good tensile strength(305.8 MPa)and fracture elongation(8.5%).The corresponding Cu/Al joint brazed using Al−10Si−2Ni−6Cu filler was mainly composed of Al_(8)(Mn,Fe)_(2)Si,Al_(2)(Cu,Ni)3,Al(Cu,Ni),Al_(2)(Cu,Ni)and Al(s,s),yielding a shear strength of(90.3±10.7)MPa.The joint strength was further improved to(94.6±2.5)MPa when the joint was brazed using the Al−10Si−2Ni−6Cu−0.2Er−0.2Zr filler alloy.Consequently,the(Cu,Ni,Zr,Er)-modified Al−Si filler alloy was suitable for obtaining high-quality Cu/Al brazed joints.

Mechanism of Zr in in situ-synthesized particle reinforced composite coatings by laser cladding

By adding mixture of ZrOand carbon, a Zrenhanced composite coating was produced onto an AISI1045 substrate by laser cladding. The microstructure and phase formation, microhardness and wear resistance of the composite coating were studied. The experimental results indicate that the composite coating with metallurgical bonding to substrate consists of y-Ni, massive ceramic particles of ZrC,NiZr, NiZr,(Fe,Ni)Cand FeC. The in situ-synthesized ZrC particles are uniformly dispersed in composite coating, which refines the microstructure of composite coating. With different Zr02 and carbon additions, the properties are improved differently. Finally, the fine in situ ZrC particles improve the microhardness of composite coating to HV650, which is nearly 2.7 times that of Ni25 coating. Also, the composite coating has an advantage in wear resistance; it offers better wear resistance when more mixture of ZrOand carbon was added in nickel alloys.

Thermal error modeling based on BiLSTM deep learning for CNC machine tool

The machining accuracy of computer numerical control machine tools has always been a focus of the manufacturing industry.Among all errors,thermal error affects the machining accuracy considerably.Because of the significant impact of Industry 4.0 on machine tools,existing thermal error modeling methods have encountered unprecedented challenges in terms of model complexity and capability of dealing with a large number of time series data.A thermal error modeling method is proposed based on bidirectional long short-term memory(BiLSTM)deep learning,which has good learning ability and a strong capability to handle a large group of dynamic data.A four-layer model framework that includes BiLSTM,a feedforward neural network,and the max pooling is constructed.An elaborately designed algorithm is proposed for better and faster model training.The window length of the input sequence is selected based on the phase space reconstruction of the time series.The model prediction accuracy and model robustness were verified experimentally by three validation tests in which thermal errors predicted by the proposed model were compensated for real workpiece cutting.The average depth variation of the workpiece was reduced from approximately 50μm to less than 2μm after compensation.The reduction in maximum depth variation was more than 85%.The proposed model was proved to be feasible and effective for improving machining accuracy significantly.

Microscopic damage mechanism of SA508 Gr3 steel in ultra-high temperature creep

The lower head of reactor pressure vessel （RPV） will endure a great temperature gradient above the phase transition temperature, and the creep and fracture will be the primary failure mode for the RPV material in such a situation. The interrupted creep tests were performed on a typical RPV material, SA508 Gr3 steel, at 800 ℃. The microstructure of different creep stages was examined by scanning electron microscopy and transmission electron microscopy. The results showed that the microscopic damage is mainly induced by creep cavities and coarse second-phase particles. Furthermore, the volume fractions of creep cavities and coarse second-phase particles show a linear relationship with the extended creep time. The second-phase particles are determined to be MoC in the second creep stage and Mo2C in the third creep stage, according to the results of selected-area electron diffraction pattern. Combined with energy-dispersive spectrum analysis, the segregation of precipitates caused by the migration of atoms is finally unveiled, which leads to the coarsening of the particles.

Brainstem auditory evoked potential combined with high resolution cranial base CT can optimize the diagnosis of auditory nerve injury

Purpose:Auditory nerve injury is one of the most common nerve injury complications of skull base fractures.However,there is currently a lack of auxiliary examination methods for its direct diagnosis.The purpose of this study was to find a more efficient and accurate means of diagnosis for auditory nerve injury.Methods:Through retrospectively analyzing the results of brainstem auditory evoked potential(BAEP)and high-resolution CT(HRCT)in 37 patients with hearing impairment following trauma from January 1,2018 to July 31,2020,the role of the two inspection methods in the diagnosis of auditory nerve injury was studied.Inclusion criteria were patient had a clear history of trauma and unilateral hearing impairment after trauma;while exclusion criteria were:(1)severe patient with a Glasgow coma scale score<5 because these patients were classified as severe head injury and admitted to the intensive care unit,(2)patient in the subacute stage admitted 72 h after trauma,and(3)patient with prior hearing impairment before trauma.According to Goodman's classification of hearing impairment,the patients were divided into low/medium/severe injury groups.In addition,patients were divided into HRCT-positive and negative groups for further investigation with their BAEP results.The positive rates of BEAP for each group were observed,and the results were analyzed by Chi-square test(p<0.05,regarded as statistical difference).Results:A total of 37 patients were included,including 21 males and 16 females.All of them were hospitalized patients with GCS score of 6-15 at the time of admission.The BAEP positive rate in the medium and severe injury group was 100%,which was significantly higher than that in the low injury group(27.27%)(p<0.01).The rate of BEAP positivity was significantly higher in the HRCT-positive group(20/30,66.7%)than in the HRCT-negative group(1/7,14.3%)(p<0.05).Twenty patients(54.05%)were both positive for BEAP and HRCT test,and considered to have auditory nerve damage.Six patients(16.22%)were both negative for BEAP and HRCT test,and 10 patients(27.03%)were BAEP-negative but HRCT-positive:all the 16 patients were considered as non-neurological injury.The rest 1 case(2.70%)was BAEP-positive but HRCT-negative,which we speculate may have auditory nerve concussion.Conclusion:By way of BAEP combining with skull base HRCT,we may improve the accuracy of the diagnosis of auditory nerve injury.Such a diagnostic strategy may be beneficial to guiding treatment plans and evaluating prognosis.

Direct C–H amination for indole synthesis from N-Ts-2-Styrylaniline derivatives catalyzed by copper salt

A direct C–H amination reaction of N-Ts-2-Styrylaniline derivatives to realize the synthesis of indole derivatives was developed in the presence of copper salt. A variety of N-Ts-2-Styrylaniline derivatives were transformed into the corresponding indole products in good to excellent yield under mild conditions with the oxidation of potassium persulfate.

Numerical analysis of reasons for the CO distribution in an opposite-wall-firing furnace

In practical operations,the carbon monoxide(CO)distribution in an opposite-wall-firing furnace(OWFF)is characterized by a high concentration near the side walls and a low concentration in the center,accompanied by a series of combustionrelated issues.To find the reasons for the CO distribution,a numerical study was conducted on a 660 MWe OWFF.The CO concentration profiles,distribution coefficients of coal and air,mixing coefficients,and the aerodynamic characteristics were extracted for analysis.The CO distribution within the furnace greatly depends on the mixing of coal and air.A mismatch between the aerodynamic behaviors of coal and air causes the non-uniform distribution of CO.Taking into consideration that distinctive flow patterns exist within the different regions,the formation mechanisms of the CO distribution can be divided into two components:(1)In the burner region,the collision of opposite flows leads to the migration of gas and particles toward the side wall which,together with the vortexes formed at furnace corners,is responsible for unburned particles concentrated and oxygenized from the furnace center to the side wall.Thus,high CO concentrations appear in these areas.(2)As the over-fire air(OFA)jet is injected into the furnace,it occupies the central region of furnace and pushes the gas from the burner region outward to the side wall,which is disadvantageous for the mixing effect in the side wall region.As a consequence,a U-shaped distribution of CO concentration is formed.Our results contribute to a theoretical basis for facilitating the control of variation in CO concentration within the furnace.

燃煤烟气半干法脱氯的化学动力学模拟（英文）

目的:燃煤锅炉烟气中的HCl对环境、设备及脱硫系统有不利的影响,也是脱硫废水处理的难点。本文提出半干法脱氯技术,探讨其技术合理性,并研究不同因素对反应效率的影响,为进一步工程应用提供理论依据,实现燃煤烟气中HCl的脱除及脱硫废水零排放。创新点:1.提出半干法脱氯技术,将氯离子固化到飞灰中;2.脱氯后脱硫废水大幅减少,将脱硫废水作为碱基溶剂回喷到烟道中,实现脱硫废水零排放;3.建立良搅拌反应器(PSR)模型,探讨半干法脱氯过程的化学动力学反应机理与关键参数。方法:1.通过CHEMKIN构建PSR模型,模拟实际燃煤烟气组分下的半干法脱氯过程;2.通过敏感性分析,探讨SO_2与HCl之间的竞争关系(图8~10);3.通过单变量模拟,研究不同因素对反应效率的影响(图13~16)。结论:1.烟气中的SO_2对HCl的脱除存在较大竞争关系,但即使Na/Cl(摩尔比)为1时,HCl的反应效率依然可观。2.HCl的反应效率与Na/Cl呈正相关;综合考虑反应效率和运行成本,设置Na/Cl为5较为合理。3.SO_2浓度的小范围变化不会对HCl的反应效率造成显著影响。4.仅通过化学动力学模拟,烟气温度对HCl的反应效率影响不明显。5.烟气中酸性气体与NaOH在0.1 s内即可完成反应,在1 s左右达到平衡。

Iron-catalyzed synthesis of polycyclic-fused azo[l,2-a]indolones via an S_NAr/acylation cascade reactions

An efficient method has been developed for the cascade synthesis of azo[1,2-a]indolones from azoles and2-fluoroaldehydes based on an iron-catalyzed SNAr and a direct acylation reaction.A number of azo[1,2-a]indolones containing different azole rings and substituents were obtained in good yields.