Data collection with microcrystals at synchrotron radiation facilities is challenging because it is difficult to harvest and locate microcrystals. Moreover,microcrystals are sensitive to radiation damage; thus, typica...Data collection with microcrystals at synchrotron radiation facilities is challenging because it is difficult to harvest and locate microcrystals. Moreover,microcrystals are sensitive to radiation damage; thus, typically, a complete data set cannot be obtained with a single microcrystal. Herein, we report a new method for data collection with multiple microcrystals having a crystal size ranging from 1 to 30 lm. This method is suitable for not only low-temperature(100 K) data collection but also room-temperature data collection. Thin Kapton membranes were used to capture multiple crystals simultaneously. The microcrystals were visible under an optical microscope and easily located because the membrane was transparent and sufficiently thin. The film was fixed to a bracket that was prepared using a three-dimensional printer. The bracket was fixed on a magnetic base via screwing and employed by the goniometer system for data collection. Multiple data sets of fatty acid-binding protein 4(FABP4) and lysozyme microcrystals were collected using this novel designed device. Finally, the structures of protein FABP4 and lysozyme were obtained from these data via the molecule replacement method. The data statistics reveal that this method provides a comparable result to traditional methods such as a nylon loop.展开更多
We propose a novel channel model for massive multiple-input multiple-out （MIMO） communication systems that incorporate the spherical wave-front assumption and non-stationary properties of clusters on both the array ...We propose a novel channel model for massive multiple-input multiple-out （MIMO） communication systems that incorporate the spherical wave-front assumption and non-stationary properties of clusters on both the array and time axes. Because of the large dimension of the antenna array in massive MIMO systems, the spherical wave-front is assumed to characterize near-field effects resulting in angle of arrival （AoA） shifts and Doppler frequency variations on the antenna array. Additionally, a novel visibility region method is proposed to capture the non-stationary properties of clusters at the receiver side. Combined with the birth-death process, a novel cluster evolution algorithm is proposed. The impacts of cluster evolution and the spherical wave-front assumption on the statistical properties of the channel model are investigated. Meanwhile, corresponding to the theoretical model, a simulation model with a finite number of rays that capture channel characteristics as accurately as possible is proposed. Finally, numerical analysis shows that our proposed non-stationary channel model is effective in capturing the characteristics of a massive MIMO channel.展开更多
基金supported by the Strategic Priority Research program of the Chinese Academy of Sciences(No.XDB08030101)
文摘Data collection with microcrystals at synchrotron radiation facilities is challenging because it is difficult to harvest and locate microcrystals. Moreover,microcrystals are sensitive to radiation damage; thus, typically, a complete data set cannot be obtained with a single microcrystal. Herein, we report a new method for data collection with multiple microcrystals having a crystal size ranging from 1 to 30 lm. This method is suitable for not only low-temperature(100 K) data collection but also room-temperature data collection. Thin Kapton membranes were used to capture multiple crystals simultaneously. The microcrystals were visible under an optical microscope and easily located because the membrane was transparent and sufficiently thin. The film was fixed to a bracket that was prepared using a three-dimensional printer. The bracket was fixed on a magnetic base via screwing and employed by the goniometer system for data collection. Multiple data sets of fatty acid-binding protein 4(FABP4) and lysozyme microcrystals were collected using this novel designed device. Finally, the structures of protein FABP4 and lysozyme were obtained from these data via the molecule replacement method. The data statistics reveal that this method provides a comparable result to traditional methods such as a nylon loop.
基金Project supported by the National Natural Science Foundation of China （No. 61421061） and the Huawei Innovation Research Program
文摘We propose a novel channel model for massive multiple-input multiple-out （MIMO） communication systems that incorporate the spherical wave-front assumption and non-stationary properties of clusters on both the array and time axes. Because of the large dimension of the antenna array in massive MIMO systems, the spherical wave-front is assumed to characterize near-field effects resulting in angle of arrival （AoA） shifts and Doppler frequency variations on the antenna array. Additionally, a novel visibility region method is proposed to capture the non-stationary properties of clusters at the receiver side. Combined with the birth-death process, a novel cluster evolution algorithm is proposed. The impacts of cluster evolution and the spherical wave-front assumption on the statistical properties of the channel model are investigated. Meanwhile, corresponding to the theoretical model, a simulation model with a finite number of rays that capture channel characteristics as accurately as possible is proposed. Finally, numerical analysis shows that our proposed non-stationary channel model is effective in capturing the characteristics of a massive MIMO channel.
