FAT4、CUL4、YAP在胃癌组织中的表达及其临床意义

目的探讨胃癌细胞侵袭过程中FAT4、CUL4、YAP在调控Hippo通路的作用及机制。方法免疫组织化学法检测胃癌组织及正常胃组织中FAT4、CUL4及YAP的表达情况,分析其表达水平与胃癌患者临床病理特征的关系;Western blot法测定胃癌组织及正常胃组织中FAT4、CUL4及YAP蛋白表达情况。结果胃癌细胞中FAT4、CUL4和YAP在性别、病理类型阳性表达率比较,差异无统计学意义(P>0.05),在胃癌的分化程度及淋巴结转移之间比较,差异有统计学意义(P<0.05),且淋巴结无转移、分化程度较高的FAT4、CUL4和YAP阳性表达率高;免疫组织化学发现胃癌组织中CUL4和YAP表达量较正常胃组织增多,胃癌组织中FAT4表达量较正常胃组织减少;胃癌组织中CUL4及YAP蛋白表达量高于正常胃组织,差异有统计学意义(P<0.05);胃癌组织中FAT4蛋白表达量低于正常胃组织,差异有统计学意义(P<0.05)。结论FAT4通过调控Hippo-YAP信号通路抑制胃癌细胞侵袭过程,CUL4通过调控Hippo-YAP信号通路促进胃癌细胞侵袭过程。

Optimization of hepatobiliary phase delay time of Gd-EOB-DTPA-enhanced magnetic resonance imaging for identification of hepatocellular carcinoma in patients with cirrhosis of different degrees of severity

AIM To optimize the hepatobiliary phase delay time(HBPDT) of Gd-EOB-DTPA-enhanced magnetic resonance imaging(GED-MRI) for more efficient identification of hepatocellular carcinoma(HCC) occurring in different degrees of cirrhosis assessed by Child-Pugh(CP) score.METHODS The liver parenchyma signal intensity(LPSI), the liver parenchyma(LP)/HCC signal ratios, and the visibility of HCC at HBP-DT of 5, 10, 15, 20, and 25 min(i.e., DT-5, DT-10, DT-15, DT-20, and DT-25) after injection of GdEOB-DTPA were collected and analyzed in 73 patients with cirrhosis of different degrees of severity(including 42 patients suffering from HCC) and 18 healthy adult controls.RESULTS The LPSI increased with HBP-DT more significantly in the healthy group than in the cirrhosis group(F = 17.361, P < 0.001). The LP/HCC signal ratios had a significant difference(F = 12.453, P < 0.001) among various HBP-DT points, as well as between CP-A and CP-B/C subgroups(F = 9.761, P < 0.001). The constituent ratios of HCC foci identified as obvious hypointensity(+++), moderate hypointensity(++), and mild hypointensity or isointensity(+/-) kept stable from DT-10 to DT-25: 90.6%, 9.4%, and 0.0% in the CP-A subgroup; 50.0%, 50.0%, and 0.0% in the CP-B subgroup; and 0.0%, 0.0%, and 100.0% in the CP-C subgroup, respectively.CONCLUSION The severity of liver cirrhosis has significant negative influence on the HCC visualization by GED-MRI. DT-10 is more efficient and practical than other HBP-DT points to identify most of HCC foci emerging in CP-A cirrhosis, as well as in CP-B cirrhosis; but an HBP-DT of 15 min or longer seems more appropriate than DT-10 for visualization of HCC in patients with CP-C cirrhosis.

超声内镜引导下经口胆囊穿刺引流术入路选择的实验研究

目的比较超声内镜引导下经胃窦和十二指肠球部胆囊穿刺引流术的操作难度、手术效果、术后并发症及后续胆汁对胃黏膜的影响。方法实验猪8头,将其分为经胃窦组和经十二指肠球部组,每组4头。在腹腔镜下实时观察超声内镜引导下经胃窦和十二指肠球部胆囊穿刺引流术,并于模型复制术后10 d处死实验动物,取吻合口及胃黏膜观察组织学改变。结果两组均有3头猪完全成功,两组操作成功率均为75%。经胃窦组平均操作时间短于经十二指肠球部组(21.67 min VS 26.67 min)(P<0.05),而胃黏膜损伤病理评分总分高于经十二指肠球部组(8.33分VS 6.33分)(P<0.05)。结论猪动物模型中,超声内镜引导下经十二指肠球部胆囊穿刺引流术操作难度稍大,但术后并发症少,较经胃窦胆囊穿刺引流术有优势。

Combined effects of cycling endurance and total ionizing dose on floating gate memory cells

The combined effects of cycling endurance and radiation on floating gate memory cell are investigated in detail,and the obtained results are listed below.(i)The programmed flash cells with a prior appropriate number of program and easing cycling stress exhibit much smaller threshold voltage shift than without those in response to radiation,which is ascribed mainly to the recombination of trapped electrons(introduced by cycling stress)and trapped holes(introduced by irradiation)in the oxide surrounding the floating gate.(ii)The radiation induced transconductance degradation in prior cycled flash cell is more severe than those without cycling stress in the programmed state or erased state.(iii)Radiation is more likely to set up the interface generation in programmed state than in erased state.This paper will be useful in understanding the issues involved in cycling endurance and radiation effects as well as in designing radiation hardened floating gate memory cells.

Analysis on degradation mechanisms of normally-off p-GaN gate AlGaN/GaN high-electron mobility transistor

The degradation mechanisms of enhancement-mode p-GaN gate AlGaN/GaN high-electron mobility transistor was analyzed extensively,by means of drain voltage stress and gate bias stress.The results indicate that:(ⅰ) High constant drain voltage stress has only a negligible impact on the device electrical parameters,with a slightly first increase and then decrease in output current;(ⅱ) A negative shift of threshold voltage and increased output current were observed in the device subjected to forward gate bias stress,which is mainly ascribed to the hole-trapping induced by high electric field across the p-GaN/AlGaN interface;(ⅲ) The analyzed device showed an excellent behavior at reverse gate bias stress,with almost unaltered threshold voltage,output current,and gate leakage current,exhibiting a large gate swing in the negative direction.The results are meaningful and valuable in directing the process optimization towards a high voltage and high reliable enhanced AlGaN/GaN high-electron mobility transistor.

Correlation analysis between underwater noise and Liutex for DTMB4119 propeller

Propeller is an important equipment of ocean structures.It has characteristics of complex geometry and periodic vortex shed,which means high research value.This paper takes DTMB419 propeller as the research object.A detailed study is conducted on the wake vortices in non-cavitation state and cavitation state.Three vortex identification methods are used to compare the vortices capture effects from downstream sections and three-dimensional perspectives.The results show that,the third-generation vortex identification technologyΩ_(R)shows obvious advantages.At the same time,FW-H equation is used to predict propeller noise,and the relationship between nonlinear sound source distribution and vortices distribution is analyzed.It is found that the distribution of Lighthill tensor is consistent withΩ_(R),which proves the advantages and potential of third-generation vortex identification technology from acoustic perspective.

Numerical simulations of FPSO with sloshing tanks in a random freak waves

This paper presents a potential-viscous coupled method to simulate a floating production storage and offloading(FPSO)with two liquefied natural gas(LNG)sloshing tanks in a random freak wave.The potential theory uses high-order-spectral(HOS)method.The random wave is generated by HOS for 2000 s,and a freak wave is observed around 1830 s.The FPSO LNG model was firstly verified in regular waves using HOS-computational fluid dynamics(CFD)coupled method and validated the HOS-CFD coupled method can solve the ship motion coupled with sloshing tanks accurately.The FPSO with LNG tanks are then simulated in the freak wave,showed the coupling effects when the freak wave passes.With the existence of the sloshing tanks,the amplitude of the roll motion decreases and the period of roll motion changed.Wave breaking phenomenon can be observed when the wave crest of the freak wave encounters with the ship.

Preliminary control of the air entrainment in bow wave based on the Liutex force method

Trails induced by air entrainment of bow wave breaking can be observed clearly around an advancing ship,making it prong to be detected and causing reduction in hydrodynamic performance.In breaking bow wave region,different scale of coherent vortex structures related closely to the air entrainment are generated.The formation and evolution of bubble clouds can be accounted partly by the swirling vortex flow of the jet plunging.In this work,high-fidelity simulation of the bow wave breaking around a rectangular thin plate is performed with the in-house code BAMR-SJTU.A Liutex force model is constructed to investigate the interaction between the free surface and vortices,and to explore the possibility of alleviating or controlling the air entrainment.This study is the first attempt to manipulate vortices corresponding to the air entrainment of the bow wave breaking.Some typical vortex structures based on the Liutex vortex identification method and the distribution of the vortex force are presented.Comparison of the vortex structures and the entrapped bubbles is conducted to illustrate the efficiency of Liutex force model in air entrainment control.

Synthesis of unnatural N-glycosyl α-amino acids via Petasis reaction

A convenient and efficient protocol for the synthesis of unnatural N-glycosyl cz-amino acids was developed. Condensation of 1,3,4,6-tetra-O-actyl-/%D-glucosamine hydrochloride, alkenyl boronic acid, and glyoxylic acid was achieved in CH2C12 to give the derivatives of 2-（N-glycosyl）aminobut-3-enoic acid which may find applications in glycobiology research and medicinal chemistry.