In terms of the electromagnetic wave measurement while drilling,the extremely low frequency electromagnetic(ELF-EM)signal below 20Hz is usually chosen as the downhole data carrier.To improve the transmission rate of E...In terms of the electromagnetic wave measurement while drilling,the extremely low frequency electromagnetic(ELF-EM)signal below 20Hz is usually chosen as the downhole data carrier.To improve the transmission rate of ELF-EM signal and the signal to noise ratio(SNR)at the receiving end,the DQPSK modulation was proposed as the modulation method for the communication of electromagnetic wave system.Different from the traditional IQ orthogonal modulation and coherent demodulation methods,the proposed phase selection modulation and correlation algorithm demodulation are easier to implement and more practical.With regard to the communication synchronization,a fast algorithm,which based on the normalized cross-relation number,was used for waveform matching,and the maximum point of the correlation coefficient was used as the starting point of communication synchronization.The communication simulation results show that the proposed DQPSK modulation signal based on the adaptive combined filtering algorithm has better terminal error rate and transmission rate than the traditional modulation method.Under the same carrier frequency and code width,the transmission rate of DQPSK modulation is 4 to 5 times and 2 times that of PPM modulation and 2DPSK modulation respectively.The communication modulation and demodulation modes as well as the decoding algorithm with combined adaptive filter proposed in this paper can effectively solve practical engineering problems.展开更多
Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China.To increase the availability of DNA markers in B.rapa,we developed insertion-deletion(InDel)markers utilizing high-r...Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China.To increase the availability of DNA markers in B.rapa,we developed insertion-deletion(InDel)markers utilizing high-resolution melting(HRM)curve analysis.We designed primers for 252 InDels(≥3 bp)evenly distributed in the genome and tested gene polymorphisms with eight accessions.In total,208 markers were specifically amplified,and 148 InDels with polymorphism were genotyped successfully using HRM.We further analyzed the correlation with InDel size,GC number,and predicted the difference in Tm values(Tm)using 208 markers with specific amplification.We found that the success rate of InDel markers was correlated with the GC number of InDel and the predicted-Tm,but not clearly correlated with the length of InDel.When the GC number within InDel was≥8,the successful rate exceeded 90.0%.When the predicted-Tm reached 0.5°C,the success rate was greater than 90.0%,and when it was≥0.6°C,the rate climbed to 100.0%,indicating their role as the optimal parameter for successful development of an applicable InDel marker.The polymorphic InDel markers can be easily genotyped using HRM.They are of great value in genetic analysis,construction of linkage map,and molecular marker-assisted selection in B.rapa.展开更多
A mono-funtionalised tetraphenylporphyrin (TPP) bearing valine moiety at the phenylring was synthesized for photocytotoxicity examination in four steps, starting from regiospecificmono-nitration of TPP at the phenyl r...A mono-funtionalised tetraphenylporphyrin (TPP) bearing valine moiety at the phenylring was synthesized for photocytotoxicity examination in four steps, starting from regiospecificmono-nitration of TPP at the phenyl ring. The in vitro photocytotoxicitic effect against SPC-Aladenocarcinona cell line was tested.展开更多
A new economic and convenient method to modify the surface of microporous polypropylene (PP) membranes with phospholipid polymer was given. The process included the photo-irradiated graft polymerization of N,N-dimethy...A new economic and convenient method to modify the surface of microporous polypropylene (PP) membranes with phospholipid polymer was given. The process included the photo-irradiated graft polymerization of N,N-dimethylaminoethyl methacrylate (DMAEMA) and the ring-opening reaction of the grafted polyDMAEMA with 2-alkyloxy-2-oxide-1,3,2-dioxo-phospholanes (AOP). Four AOPs, whose alkyloxy groups consisted of dodecyl, tetradecyl, hexadecyl and octadecyl moieties, were used to convert the grafted polyDMAEMA to phospholipid polymers. FT-IR spectra confirmed the chemical change of membrane surface. Platelets adhesion experiment indicated that PP membrane with excellent blood compatible surface could be fabricated by this method.展开更多
基金supported by National Natural Science Foundation of China(No.61771366)Stable-Support Scientific Project of China Research Institute of Radiowave Propagation(Grant No.A132201068 and No.A132107W08)。
文摘In terms of the electromagnetic wave measurement while drilling,the extremely low frequency electromagnetic(ELF-EM)signal below 20Hz is usually chosen as the downhole data carrier.To improve the transmission rate of ELF-EM signal and the signal to noise ratio(SNR)at the receiving end,the DQPSK modulation was proposed as the modulation method for the communication of electromagnetic wave system.Different from the traditional IQ orthogonal modulation and coherent demodulation methods,the proposed phase selection modulation and correlation algorithm demodulation are easier to implement and more practical.With regard to the communication synchronization,a fast algorithm,which based on the normalized cross-relation number,was used for waveform matching,and the maximum point of the correlation coefficient was used as the starting point of communication synchronization.The communication simulation results show that the proposed DQPSK modulation signal based on the adaptive combined filtering algorithm has better terminal error rate and transmission rate than the traditional modulation method.Under the same carrier frequency and code width,the transmission rate of DQPSK modulation is 4 to 5 times and 2 times that of PPM modulation and 2DPSK modulation respectively.The communication modulation and demodulation modes as well as the decoding algorithm with combined adaptive filter proposed in this paper can effectively solve practical engineering problems.
基金This work is supported by the National Natural Science Foundation of China(Grant No.31630068)the National Program on Key Research Project(Grant No.2016YFD0100307)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences,the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,China.
文摘Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China.To increase the availability of DNA markers in B.rapa,we developed insertion-deletion(InDel)markers utilizing high-resolution melting(HRM)curve analysis.We designed primers for 252 InDels(≥3 bp)evenly distributed in the genome and tested gene polymorphisms with eight accessions.In total,208 markers were specifically amplified,and 148 InDels with polymorphism were genotyped successfully using HRM.We further analyzed the correlation with InDel size,GC number,and predicted the difference in Tm values(Tm)using 208 markers with specific amplification.We found that the success rate of InDel markers was correlated with the GC number of InDel and the predicted-Tm,but not clearly correlated with the length of InDel.When the GC number within InDel was≥8,the successful rate exceeded 90.0%.When the predicted-Tm reached 0.5°C,the success rate was greater than 90.0%,and when it was≥0.6°C,the rate climbed to 100.0%,indicating their role as the optimal parameter for successful development of an applicable InDel marker.The polymorphic InDel markers can be easily genotyped using HRM.They are of great value in genetic analysis,construction of linkage map,and molecular marker-assisted selection in B.rapa.
文摘A mono-funtionalised tetraphenylporphyrin (TPP) bearing valine moiety at the phenylring was synthesized for photocytotoxicity examination in four steps, starting from regiospecificmono-nitration of TPP at the phenyl ring. The in vitro photocytotoxicitic effect against SPC-Aladenocarcinona cell line was tested.
文摘A new economic and convenient method to modify the surface of microporous polypropylene (PP) membranes with phospholipid polymer was given. The process included the photo-irradiated graft polymerization of N,N-dimethylaminoethyl methacrylate (DMAEMA) and the ring-opening reaction of the grafted polyDMAEMA with 2-alkyloxy-2-oxide-1,3,2-dioxo-phospholanes (AOP). Four AOPs, whose alkyloxy groups consisted of dodecyl, tetradecyl, hexadecyl and octadecyl moieties, were used to convert the grafted polyDMAEMA to phospholipid polymers. FT-IR spectra confirmed the chemical change of membrane surface. Platelets adhesion experiment indicated that PP membrane with excellent blood compatible surface could be fabricated by this method.