[Objectives] To establish fingerprint chromatogram of Tetracera asiatica and provide basis for controlling and assessing the quality of Tetracera asiatica.[Methods] The high-performance liquid chromatography( HPLC) me...[Objectives] To establish fingerprint chromatogram of Tetracera asiatica and provide basis for controlling and assessing the quality of Tetracera asiatica.[Methods] The high-performance liquid chromatography( HPLC) method was used. Phenomenex C18( 5 μm,4. 6 ×250 mm),acetonitrile-0. 1% phosphoric acid water,gradient elution,flow rate 1 m L/min,detection wavelength 214 nm,and column temperature 25 ℃. The fingerprint chromatogram of 10 batches of samples of Tetracera asiatica was determined for similarity comparison. [Results] It established HPLC fingerprint chromatogram of Tetracera asiatica produced in Guangxi,determined a total of 13 common fingerprint peaks. The similarity of 10 batches of Tetracera asiatica was > 0. 9.[Conclusions] The method is rapid and accurate and can provide references for assessing the quality of Tetracera asiatica.展开更多
[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta ...[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta were selected through digestion,ligation,suppression PCR,pre-amplification,SAM method and PCR. [Results] The improved CTAB method extracted a high purity of DNA; the SAM PCR method gave clear bands; and 100 positive clones were sequenced,obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for analyzing the genetic diversity of M. convoluta.展开更多
[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-l...[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards.展开更多
This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years,different periods,different...This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years,different periods,different habitats and different external factors had influences on the change of polysaccharide content in Dendrobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity,resisting fatigue and oxidation,promoting digestion and salivation secretion,reducing blood sugar and blood pressure,resisting tumor and liver injury,and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material.展开更多
[Objectives]The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi.[Methods]The paraffin sect...[Objectives]The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi.[Methods]The paraffin sections of D.officinale were made,and the microscopic characteristic constants of D.officinale stem,including cross section area,vascular bundle area and phloem area,were determined by NIS-Elements D microscopic image processing software.The contents of polysaccharides in D.officinale were determined by UV-Vis spectrophotometry,and the quantitative values of microscopic characteristics of D.officinale with different growth years were analyzed by SPSS 23.0(IBM SPSS Statistics 23.0)data statistical software.The correlation between quantitative microscopic characteristics of D.officinale and its polysaccharide content was analyzed.[Results]According to the correlation between the index component of D.officinale and its quantitative microscopic characteristics,the measurement indexes,the percentage of vascular bundle in the cross section area and the percentage of hard structure in the cross section area,were strongly negatively correlated with polysaccharide content.[Conclusions]The microscopic characteristic constant values of vascular bundles of D.officinale can be used as a method to verify polysaccharide content.展开更多
基金Supported by Natural Science Foundation Project of Guangxi(2016GXNSFBA380014)Program of Self-raised Fund of Ethnic Medicine of Guangxi Traditional Chinese Medicine(GZZC16-24)+5 种基金Scientific Research Project of Institutions of Higher Learning in Guangxi(KY2015YB160)Scientific Research Project of Guangxi University of Traditional Chinese Medicine(2015LX005)Key Laboratory Project of Guangxi Zhuang and Yao Medicine(Gui Ke Ji Zi:2014,No.32)Collaborative Innovation Center Project of Zhuang and Yao Medicine(Gui Jiao Ke Yan:2013,No.20)Guangxi Key Discipline(Zhuang Medicine Study)Project(Gui Jiao Ke Yan:2013,No.16)Bagui Scholar Program"Innovation Theory and Efficacy Studies of Traditional Chinese Medicine"
文摘[Objectives] To establish fingerprint chromatogram of Tetracera asiatica and provide basis for controlling and assessing the quality of Tetracera asiatica.[Methods] The high-performance liquid chromatography( HPLC) method was used. Phenomenex C18( 5 μm,4. 6 ×250 mm),acetonitrile-0. 1% phosphoric acid water,gradient elution,flow rate 1 m L/min,detection wavelength 214 nm,and column temperature 25 ℃. The fingerprint chromatogram of 10 batches of samples of Tetracera asiatica was determined for similarity comparison. [Results] It established HPLC fingerprint chromatogram of Tetracera asiatica produced in Guangxi,determined a total of 13 common fingerprint peaks. The similarity of 10 batches of Tetracera asiatica was > 0. 9.[Conclusions] The method is rapid and accurate and can provide references for assessing the quality of Tetracera asiatica.
基金Supported by Natural Science Foundation of China(No.81260620)Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug Efficacy Study of Bagui Scholars
文摘[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta were selected through digestion,ligation,suppression PCR,pre-amplification,SAM method and PCR. [Results] The improved CTAB method extracted a high purity of DNA; the SAM PCR method gave clear bands; and 100 positive clones were sequenced,obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for analyzing the genetic diversity of M. convoluta.
基金Supported by Guangxi"2011 Collaborative Innovation Center"-Zhuang Yao Medicine Collaborative Innovation Center Project(GJKY[2013]20)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+7 种基金Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Bagui Scholars ProjectNational Natural Science Foundation of China(81460587)Nanning Science and Technology Bureau Project(20183046-1)Project of Guangxi Association for Science and TechnologyThe Fourth National General Survey of Chinese Medicine Resources(Guangxi)Pilot Survey Project(GXZYZYPC-2)Guangxi Marine Drug Resources Survey-Qinzhou City(GXZYZYPC13-7-2)Guangxi First-class Discipline:Chinese Pharmacy(0501802803)
文摘[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards.
基金Supported by National Natural Science Foundation of China(81460587)Zhuang Yao Medicine Collaborative InnovationC enter Project(GJKY[2013]20)+2 种基金Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)Guangxi Key Discipline:Zhuang Pharmacy(GJKY[2013]16)Chinese Traditional Medicine Innovation Theory and Drug EfficacyS tudy of Bagui Scholars
文摘This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years,different periods,different habitats and different external factors had influences on the change of polysaccharide content in Dendrobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity,resisting fatigue and oxidation,promoting digestion and salivation secretion,reducing blood sugar and blood pressure,resisting tumor and liver injury,and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material.
基金Supported by 2020 Operation Subsidy Project of Guangxi Key Research Laboratory of Zhuang&Yao Medicine(20-065-14)"Quantification of Microscopic Characteristics of Dendrobium officinale from Guangxi and Its Correlation with the Quality of Medicinal Materials"(GXZYYKF2020A-13)2011 Guangxi Collaborative Innovation Center"Pharmaceutical Collaborative Innovation Center of Zhuang&Yao Medicine"(GJKY[2013]20)+6 种基金Guangxi Engineering Research Center for Resources and Application of Ethnic Medicine(GFGGJH[2020]2605)National Natural Science Foundation of China(82060695)Joint Research Laboratory of Zhuang&Yao Medicine of Guangxi University of Traditional Chinese Medicine and Central South University(GKJZ[2021]238)Guangxi Science and Technology Base and Talent Project(GK AD19245090)2018 Research Start-up Fund Project of Guangxi University of Chinese Medicine for Introduced Doctors(2018BS019)2019 Basic Scientific Research Ability Improvement Project for Young and Middle-aged Teachers in Colleges and Universities in Guangxi(2019KY0310)2019-2021 Guangxi First-class Discipline Construction Open Project of Guangxi University of Traditional Chinese Medicine(2019XK101)。
文摘[Objectives]The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi.[Methods]The paraffin sections of D.officinale were made,and the microscopic characteristic constants of D.officinale stem,including cross section area,vascular bundle area and phloem area,were determined by NIS-Elements D microscopic image processing software.The contents of polysaccharides in D.officinale were determined by UV-Vis spectrophotometry,and the quantitative values of microscopic characteristics of D.officinale with different growth years were analyzed by SPSS 23.0(IBM SPSS Statistics 23.0)data statistical software.The correlation between quantitative microscopic characteristics of D.officinale and its polysaccharide content was analyzed.[Results]According to the correlation between the index component of D.officinale and its quantitative microscopic characteristics,the measurement indexes,the percentage of vascular bundle in the cross section area and the percentage of hard structure in the cross section area,were strongly negatively correlated with polysaccharide content.[Conclusions]The microscopic characteristic constant values of vascular bundles of D.officinale can be used as a method to verify polysaccharide content.