Objective:In various cancers,migration and invasion inhibitory protein(MIIP)is expressed at low level and is involved in cancer pathogenesis.Herein,we sought to explore the function of MIIP in clear cell renal cell ca...Objective:In various cancers,migration and invasion inhibitory protein(MIIP)is expressed at low level and is involved in cancer pathogenesis.Herein,we sought to explore the function of MIIP in clear cell renal cell carcinoma(ccRCC).Methods:CCK-8,colony formation,cell cycle,and endothelial cell tube formation assays were performed to evaluate the roles of MIIP in ccRCC proliferation and angiogenesis.To explore the underlyi ng mechanism,we con ducted RNA-sequencing,GSEA,qRT-PCR,Western blot,ELISA,cell transfection,coimmunoprecipitation,and ubiquitination assays in ccRCC cell lines.Furthermore,xenograft tumor growth in nude mice,and Ki-67 and CD31 staining in xenograft tissues were examined.Finally,the association of MIIP expression with clinical pathology and the expression status of HIF-2a and cysteine-rich 61(CYR61)were further analyzed in human RCC tissues through Western blot and immunohistochemistry.Results:Both in vitro and in vivo functional experiments indicated that forced expression of MIIP inhibited ccRCC proliferation and angiogenesis,whereas silencing MIIP either in normal HK-2 cells or in ccRCC cells had the opposite effect(P<0.05).Mechanistically,CYR61 was identified as a gene significantly downregulated by MIIP overexpression,and was required for the suppressive role of MIIP in ccRCC.MIIP was found to promote HSP90 acetylation and thus impair its chaperone function toward HIF-2a.Consequently,RACK1 binds HIF-2a and causes its ubiquitination and proteasomal degradation,thus decreasing the transcription of its target,CYR61.Finally,analyses of clinical samples demonstrated that MIIP is significantly downregulated in cancer vs.normal tissues in RCC cases,and its expression is negatively associated with histological grade,metastasis,the prognosis of patients with RCC,and the expression of HIF-2a and CYR61(P<0.05).Conclusions:MIIP is a novel tumor suppressor in ccRCC via negative regulation of HIF-2a-CYR61 axis.展开更多
The Evaluation of teaching quality is a complicated project. It’s a typical problem of uncertainty. A design scheme of teaching quality evaluation method based on cloud model and D-S theory is proposed with the schoo...The Evaluation of teaching quality is a complicated project. It’s a typical problem of uncertainty. A design scheme of teaching quality evaluation method based on cloud model and D-S theory is proposed with the school teaching evaluation index. With the experiences and knowledge of experts, evaluation index was described by cloud model and judgment matrix of AHP was revised by D-S theory. The weight of evaluation indexes was determined reasonably and objectively. Applied in the actual environment, the method can make an accurate and efficient evaluation with a certain practical value.展开更多
Digital exchange platform is a comprehensive service platform. In this paper, the construction scheme of the platform is proposed. By constructing the data exchange platform, a people-oriented information environment ...Digital exchange platform is a comprehensive service platform. In this paper, the construction scheme of the platform is proposed. By constructing the data exchange platform, a people-oriented information environment was built to meet demands of faculty, staff and students on information resources. And thus a digital campus was constructed to provide efficient online services and information resources. The experimental results show that the platform is user-friendly, runs orderly, integrates the service, and provides a good reference value for the data integration of enterprises and universities in the future.展开更多
Because higher education in China attaches more importance to theory rather than to practice, there is a gap between academic research and technical practice. With transformation and development of universities, to cu...Because higher education in China attaches more importance to theory rather than to practice, there is a gap between academic research and technical practice. With transformation and development of universities, to cultivate a large number of technical talents who can make scientific and technological achievements into practice, an educating strategy of integrating enterprises into higher education is proposed. From universities’ perspective, the cooperation with enterprises was further strengthened by linking the Industry- University-Research cooperation. With university-enterprise cooperation to indepth integration, the talent supply was further improved. And an in-depth ecological mechanism of government-led, market-driven and common development between universities and enterprises was established. The research result show that with the proposed strategy “win-win” cooperation between universities and enterprises is achieved.展开更多
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.82002686,81872421,81502405,31700667)Science Technology Research and Development Program of Shaanxi Province(Grant No.2008k09-04-05)Basic Research Program of Natural Science of Shaanxi Province(Grant No.2019JM-315).
文摘Objective:In various cancers,migration and invasion inhibitory protein(MIIP)is expressed at low level and is involved in cancer pathogenesis.Herein,we sought to explore the function of MIIP in clear cell renal cell carcinoma(ccRCC).Methods:CCK-8,colony formation,cell cycle,and endothelial cell tube formation assays were performed to evaluate the roles of MIIP in ccRCC proliferation and angiogenesis.To explore the underlyi ng mechanism,we con ducted RNA-sequencing,GSEA,qRT-PCR,Western blot,ELISA,cell transfection,coimmunoprecipitation,and ubiquitination assays in ccRCC cell lines.Furthermore,xenograft tumor growth in nude mice,and Ki-67 and CD31 staining in xenograft tissues were examined.Finally,the association of MIIP expression with clinical pathology and the expression status of HIF-2a and cysteine-rich 61(CYR61)were further analyzed in human RCC tissues through Western blot and immunohistochemistry.Results:Both in vitro and in vivo functional experiments indicated that forced expression of MIIP inhibited ccRCC proliferation and angiogenesis,whereas silencing MIIP either in normal HK-2 cells or in ccRCC cells had the opposite effect(P<0.05).Mechanistically,CYR61 was identified as a gene significantly downregulated by MIIP overexpression,and was required for the suppressive role of MIIP in ccRCC.MIIP was found to promote HSP90 acetylation and thus impair its chaperone function toward HIF-2a.Consequently,RACK1 binds HIF-2a and causes its ubiquitination and proteasomal degradation,thus decreasing the transcription of its target,CYR61.Finally,analyses of clinical samples demonstrated that MIIP is significantly downregulated in cancer vs.normal tissues in RCC cases,and its expression is negatively associated with histological grade,metastasis,the prognosis of patients with RCC,and the expression of HIF-2a and CYR61(P<0.05).Conclusions:MIIP is a novel tumor suppressor in ccRCC via negative regulation of HIF-2a-CYR61 axis.
文摘The Evaluation of teaching quality is a complicated project. It’s a typical problem of uncertainty. A design scheme of teaching quality evaluation method based on cloud model and D-S theory is proposed with the school teaching evaluation index. With the experiences and knowledge of experts, evaluation index was described by cloud model and judgment matrix of AHP was revised by D-S theory. The weight of evaluation indexes was determined reasonably and objectively. Applied in the actual environment, the method can make an accurate and efficient evaluation with a certain practical value.
文摘Digital exchange platform is a comprehensive service platform. In this paper, the construction scheme of the platform is proposed. By constructing the data exchange platform, a people-oriented information environment was built to meet demands of faculty, staff and students on information resources. And thus a digital campus was constructed to provide efficient online services and information resources. The experimental results show that the platform is user-friendly, runs orderly, integrates the service, and provides a good reference value for the data integration of enterprises and universities in the future.
文摘Because higher education in China attaches more importance to theory rather than to practice, there is a gap between academic research and technical practice. With transformation and development of universities, to cultivate a large number of technical talents who can make scientific and technological achievements into practice, an educating strategy of integrating enterprises into higher education is proposed. From universities’ perspective, the cooperation with enterprises was further strengthened by linking the Industry- University-Research cooperation. With university-enterprise cooperation to indepth integration, the talent supply was further improved. And an in-depth ecological mechanism of government-led, market-driven and common development between universities and enterprises was established. The research result show that with the proposed strategy “win-win” cooperation between universities and enterprises is achieved.
