Using lactic acid bacteria(LAB)to ferment Huyou peel and pomace could provide a reference for making full use of Huyou resources and provide valuable suggestions for the industrial production of functional foods.The e...Using lactic acid bacteria(LAB)to ferment Huyou peel and pomace could provide a reference for making full use of Huyou resources and provide valuable suggestions for the industrial production of functional foods.The effects of LAB fermentation on the bioactive composition,volatile compounds and antioxidant activity of Huyou peel and pomace were studied.Results showed that both Huyou peel and pomace were good substrates for LAB growth.LAB had remarkable influence on bioconversion of organic acids,among which ascorbic acid content was increased significantly.In addition,LAB could produce flavonoids with enhanced bioactivity and absorption.In particular,the contents of rhoifolin,quercitrin and quercetin were increased by over 100%.Besides,the volatile compounds contents and aroma complexity were improved.Furthermore,the antioxidant activity was enhanced.Thus,our study provides high-value applications of Huyou,and fermented products with improved nutritional value and flavor are potentially beneficial to human health.展开更多
Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application ...Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality maintenance.Materials and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was verified.The growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/mL.Images of membrane integrity.SEM and biofilm formation suggested significant biological structure damage in bacteria after treatment.Meanwhile,the Qs signaling molecule Al-2 content showed a decline with increasing treatment concentration.Transcriptome sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying degree.Conclusion:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of strains.These synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.展开更多
基金supported by the‘Pioneer'and‘Leading Goose'R&D Program of Zhejiang(No.2022C02012),China.
文摘Using lactic acid bacteria(LAB)to ferment Huyou peel and pomace could provide a reference for making full use of Huyou resources and provide valuable suggestions for the industrial production of functional foods.The effects of LAB fermentation on the bioactive composition,volatile compounds and antioxidant activity of Huyou peel and pomace were studied.Results showed that both Huyou peel and pomace were good substrates for LAB growth.LAB had remarkable influence on bioconversion of organic acids,among which ascorbic acid content was increased significantly.In addition,LAB could produce flavonoids with enhanced bioactivity and absorption.In particular,the contents of rhoifolin,quercitrin and quercetin were increased by over 100%.Besides,the volatile compounds contents and aroma complexity were improved.Furthermore,the antioxidant activity was enhanced.Thus,our study provides high-value applications of Huyou,and fermented products with improved nutritional value and flavor are potentially beneficial to human health.
基金supported by the Hainan Provincial Natural Science Foundation of China (321CXTD1012)the National Natural Science Foundation of China (NSFC31871868)the Scientific Research Foundation of Hainan Tropical Ocean University (RHDRC202117),China.
文摘Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality maintenance.Materials and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was verified.The growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/mL.Images of membrane integrity.SEM and biofilm formation suggested significant biological structure damage in bacteria after treatment.Meanwhile,the Qs signaling molecule Al-2 content showed a decline with increasing treatment concentration.Transcriptome sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying degree.Conclusion:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of strains.These synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.
