Weld bead morphology plays an important role in welding procedure specification and welding process planning.In order to obtain the quantitative relationship between process parameters and weld morphology of DH36 mari...Weld bead morphology plays an important role in welding procedure specification and welding process planning.In order to obtain the quantitative relationship between process parameters and weld morphology of DH36 marine steel plate,grey correlation method was used to study multiple morphology characteristics simultaneously.Eighteen experimental runs were proposed according to orthogonal method.With grey correlation theory,three response objectives,aspect ratio,depth of penetration and weld were transformed into a single grey correlation grade(GRG).The process parameters were quantitatively correlated to the GRG value and optimized with the target of maximum aspect ratio,minimum depth of penetration and maximum weld area.The results show that the optimized weld bead morphology is better than other weld bead morphology.The relative error of the predicted GRG with respect to the actual value is 5.06%,which further verifies the accuracy of the established model.The microstructure and hardness analysis suggests that the optimized welded joint has obtained required mechanical properties.The research outcomes provide a theoretical basis for multi-objective optimization of arc welding morphology in practical applications.展开更多
为研究二倍体马铃薯TCP转录因子基因StBRC1a的功能,在二倍体马铃薯Solanum tuberosum group phurejaS15-65中对StBRC1a进行了克隆,发现StBRC1a有两个不同长度的转录本,分别命名为StBRC1a^L与StBRC1a^S。序列分析后发现,二者均具有TCP转...为研究二倍体马铃薯TCP转录因子基因StBRC1a的功能,在二倍体马铃薯Solanum tuberosum group phurejaS15-65中对StBRC1a进行了克隆,发现StBRC1a有两个不同长度的转录本,分别命名为StBRC1a^L与StBRC1a^S。序列分析后发现,二者均具有TCP转录因子家族特有的由59个氨基酸组成的非典型碱性—螺旋—环—螺旋(bHLH)结构域及1个R结构域。通过构建系统演化树发现,StBRC1a^L和StBRC1a^S与番茄的SlBRC1a具有最近的亲缘关系,同属于CYC/TB1类TCP转录因子。组织特异性表达分析的结果表明StBRC1a^L与StBRC1a^S在腋芽中特异性的高表达。通过CRISPR/Cas9系统构建StBRC1a功能缺失的纯合突变体后发现,与野生型相比突变体植株表现为分枝显著增多的表型,说明StBRC1a对于二倍体马铃薯的分枝具有抑制作用。展开更多
基金Natural Science Foundation of Fujian Province(Grant No.2020J01873)Technology Major Project of Fujian Province(Grant No.2020H03018).
文摘Weld bead morphology plays an important role in welding procedure specification and welding process planning.In order to obtain the quantitative relationship between process parameters and weld morphology of DH36 marine steel plate,grey correlation method was used to study multiple morphology characteristics simultaneously.Eighteen experimental runs were proposed according to orthogonal method.With grey correlation theory,three response objectives,aspect ratio,depth of penetration and weld were transformed into a single grey correlation grade(GRG).The process parameters were quantitatively correlated to the GRG value and optimized with the target of maximum aspect ratio,minimum depth of penetration and maximum weld area.The results show that the optimized weld bead morphology is better than other weld bead morphology.The relative error of the predicted GRG with respect to the actual value is 5.06%,which further verifies the accuracy of the established model.The microstructure and hardness analysis suggests that the optimized welded joint has obtained required mechanical properties.The research outcomes provide a theoretical basis for multi-objective optimization of arc welding morphology in practical applications.
文摘为研究二倍体马铃薯TCP转录因子基因StBRC1a的功能,在二倍体马铃薯Solanum tuberosum group phurejaS15-65中对StBRC1a进行了克隆,发现StBRC1a有两个不同长度的转录本,分别命名为StBRC1a^L与StBRC1a^S。序列分析后发现,二者均具有TCP转录因子家族特有的由59个氨基酸组成的非典型碱性—螺旋—环—螺旋(bHLH)结构域及1个R结构域。通过构建系统演化树发现,StBRC1a^L和StBRC1a^S与番茄的SlBRC1a具有最近的亲缘关系,同属于CYC/TB1类TCP转录因子。组织特异性表达分析的结果表明StBRC1a^L与StBRC1a^S在腋芽中特异性的高表达。通过CRISPR/Cas9系统构建StBRC1a功能缺失的纯合突变体后发现,与野生型相比突变体植株表现为分枝显著增多的表型,说明StBRC1a对于二倍体马铃薯的分枝具有抑制作用。
