This study explored the effects over time of lactic acid （LA） on IκBα phosphorylation and nuclear factor-kappa B （NF-κB） p65 protein expression, and on tumor necrosis factor a （TNF-α） and interleukin-6 （IL-...This study explored the effects over time of lactic acid （LA） on IκBα phosphorylation and nuclear factor-kappa B （NF-κB） p65 protein expression, and on tumor necrosis factor a （TNF-α） and interleukin-6 （IL-6） mRNA levels in rat intestinal mucosa microvascular endothelial cells （RIMMVECs） stimulated by lipopolysaccharide （LPS）. I？Ba, phosphorylated IκBa （p-IκBa） and p65 protein levels were monitored by Western blot analysis, and TNF-a and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF-a and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on IκBa phosphorylation to prevent activation of NF-κB was after 6 h. These results suggest that LA reduces TNF-a and IL-6 mRNA levels through decreasing IκBa phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF-κB.展开更多
The effect of on-the-job training on job mobility is an important topic in labor economics.Based on a sample survey data of migrant workers in Liaoning Province China,this paper has statistically described job mobilit...The effect of on-the-job training on job mobility is an important topic in labor economics.Based on a sample survey data of migrant workers in Liaoning Province China,this paper has statistically described job mobility of migrant workers and then used a multinomial Logistic model to analyze the effect of on-the-job training on job mobility.We found,firstly,migrant workers have high-level job mobility;secondly,inter-firm/city occupational mobility is the major type of migrant workers’mobility,and then followed by the inter-firm mobility and inter-regional mobility that without occupation change;thirdly,on-the-job training has a significant impending effect on interregional mobility and occupational mobility,while has insignificant effect on inter-firm mobility.展开更多
基金supported by the Natural Science Foundation of Beijing,China(6072007)the Project for the Establishment and Development of Swine Disease Clinical Diagnosis,China(KM200910020002)
文摘This study explored the effects over time of lactic acid （LA） on IκBα phosphorylation and nuclear factor-kappa B （NF-κB） p65 protein expression, and on tumor necrosis factor a （TNF-α） and interleukin-6 （IL-6） mRNA levels in rat intestinal mucosa microvascular endothelial cells （RIMMVECs） stimulated by lipopolysaccharide （LPS）. I？Ba, phosphorylated IκBa （p-IκBa） and p65 protein levels were monitored by Western blot analysis, and TNF-a and IL-6 mRNA levels were analyzed using real-time PCR. LA treatment reduced TNF-a and IL-6 mRNA levels in LPS-stimulated RIMMVECs, with the greatest effect being after 3 h. The highest inhibitory effect of LA on IκBa phosphorylation to prevent activation of NF-κB was after 6 h. These results suggest that LA reduces TNF-a and IL-6 mRNA levels through decreasing IκBa phosphorylation and blocking the dissociation of IKK complex, which prevents activation of NF-κB.
基金The authors are grateful for financial support from the Social Science Foundation of Liaoning Province(L16BGL038),Program for Liaoning Excellent Talents in University(WJQ2015026),the Youth Project of the Philosophy and Social Science Research,Ministry of Education of China(13YJC790057),and the National Natural Science Foundation of China(71303161,71503173).
文摘The effect of on-the-job training on job mobility is an important topic in labor economics.Based on a sample survey data of migrant workers in Liaoning Province China,this paper has statistically described job mobility of migrant workers and then used a multinomial Logistic model to analyze the effect of on-the-job training on job mobility.We found,firstly,migrant workers have high-level job mobility;secondly,inter-firm/city occupational mobility is the major type of migrant workers’mobility,and then followed by the inter-firm mobility and inter-regional mobility that without occupation change;thirdly,on-the-job training has a significant impending effect on interregional mobility and occupational mobility,while has insignificant effect on inter-firm mobility.
