The invivo study of myeloprotection by GST-π gene transfected human cord blood hematopoietic stem cells transplantation

Objective :To investigate the influence of GST πgenetransferintohumancord blood hematopoie ticstem cellson their drugres is tanceagains tanti tumordrugs invivo .Methods: GST πgenetran sfectionin to human cord blood CD 34+cells was carried outusing are trovirusvec torPLJ GST πwith the aido f fibronect in .Succes sfulgenetransfer was confirmed by invitrocolony assay and RT PCR .GST πgenetrans duced human cord blood CD34+cells were the nengrafted in to 4 week old total body irradiated NOD/Scid mice and carboplatin was intraperitoneally admin is tered sequentially at 4 weeks interval 4 week saftereng raftment.Results :Peripheral blood (PB) WBC was significantly higher in GST πmice than control mice after 2 course of car boplat in .Retroviral GST π expression in bone marrow hematopoietic progenitor cells of recipient mice was detected by RT PCR 16 weeks after Xenotransplantation .Conclusion :The transfection of GST π gene could confer ,to some extent ,resistance to cord blood stem cells against carboplatin in vivo .

Study on the activity and phenotype of decidual natural killer cells in patients with unexplained habitual abortions

Objective:To determine the activity and phenotype of decidual natural killer (NK) cells in patients with unexplained habitual abortions (UHA).Methods:A total of 32 patients with UHA were studied, and 20 cases of normal pregnant women were selected as control group. The levels of CD56 +CD3 - NK cells and their CD56 +CD16 -, CD56 +CD16 + subsets in decidua were detected using two color flow cytometric analysis.The NK cells activity was measured by a chromium 51( 51 Cr) release cytotoxicity assay,with K562 human myeloid leukaemia cells as targets.Results:Compared with control group, the proportion of CD56 +CD3 - NK cells in decidual mononuclear cells(DMC) of UHA patients had no difference, but the CD56 +CD16 - NK cell subset decreased and the CD56 +CD16 + subset increased significantly (P<0.05). The decidual NK cells activities of UHA patients were higher than those of normal controls (P<0.05).Conclusions:NK cell is predominant lymphocyte in normal decidua and plays an important role in maintaining successful pregnancy. Abnormally raised activity and disbalanced CD56 +CD16 +, CD56 +CD16 - subsets of decidual NK cell are associated with UHA and may play a role in reproductive failure.

TURBO EQUALIZATION WITH JOINTLY GAUSSIAN EQUALIZER

A Jointly Gaussian (JG) equalizer is derived for turbo equalization based on an augmented real matrix representation of channel model and a Gaussian approximation of the received symbol sequence. Using matrix inversion lemma and Cholesky decomposition, a lowcomplexity implementation of JG equalizer is also presented. The simulation results and complexity comparison confirm that turbo equalization with JG equalizer has a better performance and a lower complexity than the existing turbo equalization with linear minimum mean squared error equalizer.