The Gibberellic Acid-stimulated Arabidopsis(GASA)gene family is involved in the regulation of gene expression and plant growth,development,and stress responses.To investigate the function of loquat GASA genes in the g...The Gibberellic Acid-stimulated Arabidopsis(GASA)gene family is involved in the regulation of gene expression and plant growth,development,and stress responses.To investigate the function of loquat GASA genes in the growth and developmental regulation of plants,a loquat EjGASA6 gene homologous to Arabidopsis AtGASA6 was cloned.EjGASA6 expression was induced by gibberellin,and ectopic transgenic plants containing this gene exhibited earlier bloom and longer primary roots since these phenotypic characteristics are related to higher gibberellin content.Transcriptome analysis and qRT-PCR results showed that the expression levels of GA3ox1 and GA20ox1,which encode key enzymes in gibberellin biosynthesis,were significantly increased.Furthermore,we confirmed that EjGASA6 could promote the expression of GA20ox1 via the luciferase reporter system.Overall,our results suggest that EjGASA6 promotes blooming and main-root elongation by positively regulating gibberellin biosynthesis.These findings broaden our understanding of the role of GASAs in plant development and growth,and lay the groundwork for future research into the functions of EjGASA6 in regulating loquat growth and development.展开更多
Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wil...Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding genes.Analysis of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its divergence.Genome resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic admixture.Compared with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic diversity.However,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication process.Large-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development stages.Unlike those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit development.These high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.展开更多
Few reports have been seen on the segregation of polyploids and on the relationship between the segregation of SSR loci and that of the morphological traits in polyploid progeny. This paper attempted to gain an insigh...Few reports have been seen on the segregation of polyploids and on the relationship between the segregation of SSR loci and that of the morphological traits in polyploid progeny. This paper attempted to gain an insight into the segregation of the hexaploid tobacco progeny and to understand the correlation of the segregation of SSR loci with that of the flower morphological traits. The segregation was evaluated with both SSR markers and morphological traits of the flowers. Twenty pairs of SSR primers were screened and a total of 42 SSR loci were identified. Chi-square analysis showed that the segregation ratio of the SSR loci in the aneuploid progeny was between 1:1 and 2:1. Six morphological traits of flowers, including the lengths of the flower and the calyx, the widths of the calyx, the corolla tube and the corolla, and the coloring of the flower, from 180 progeny and 2 progenitor plants, were determined and Chi-square analyzed. All traits were in consistent also with a segregation ratio of 1:1 to 2:1 except the width of the calyx.展开更多
基金financially supported by the National Key R&D Program of China (2023YFD1600800)the National Nature Science Foundation of China (32102321)+4 种基金the Chongqing Science and Technology Commission, China (cstc2024ycjh-bgzxm0202, cstc2021jscx-gksbX0010 and cstc2021jcyj-msxmX1156)the Chongqing Forestry Administration, China (YuLinKeYan2022-14)the Innovation Research Group Funds for Chongqing Universities, China (CXQT19005)the Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission, China [(2022)164 and 2020(3)01]the Chongqing Postgraduate Research and Innovation Programme, China (CYB23128)
文摘The Gibberellic Acid-stimulated Arabidopsis(GASA)gene family is involved in the regulation of gene expression and plant growth,development,and stress responses.To investigate the function of loquat GASA genes in the growth and developmental regulation of plants,a loquat EjGASA6 gene homologous to Arabidopsis AtGASA6 was cloned.EjGASA6 expression was induced by gibberellin,and ectopic transgenic plants containing this gene exhibited earlier bloom and longer primary roots since these phenotypic characteristics are related to higher gibberellin content.Transcriptome analysis and qRT-PCR results showed that the expression levels of GA3ox1 and GA20ox1,which encode key enzymes in gibberellin biosynthesis,were significantly increased.Furthermore,we confirmed that EjGASA6 could promote the expression of GA20ox1 via the luciferase reporter system.Overall,our results suggest that EjGASA6 promotes blooming and main-root elongation by positively regulating gibberellin biosynthesis.These findings broaden our understanding of the role of GASAs in plant development and growth,and lay the groundwork for future research into the functions of EjGASA6 in regulating loquat growth and development.
基金supported by the National Key R&D Program of China(No.2019YFD1000200)the National Nature Science Foundation of China(No.32102321)+2 种基金the Chongqing Science and Technology Commission(cstc2021jcyj-msxmX1156 and cstc2021jscxgksbX0010)the Innovation Research Group Funds for Chongqing Universities(CXQT19005)the Fundamental Research Funds for the Central Universities(SWU-KT22055).
文摘Wild loquats(Eriobotrya japonica Lindl.)provide remarkable genetic resources for studying domestication and breeding improved varieties.Herein,we generate the first high-quality chromosome-level genome assembly of wild loquat,with 45791 predicted proteincoding genes.Analysis of comparative genomics indicated that loquat shares a common ancestor with apple and pear,and a recent whole-genome duplication event occurred in loquat prior to its divergence.Genome resequencing showed that the loquat germplasms can be distinctly classified into wild and cultivated groups,and the commercial cultivars have experienced allelic admixture.Compared with cultivated loquats,the wild loquat genome showed very few selected genomic regions and had higher levels of genetic diversity.However,whole-genome scans of selective sweeps were mainly related to fruit quality,size,and f lesh color during the domestication process.Large-scale transcriptome and metabolome analyses were further performed to identify differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in wild and cultivated loquats at various fruit development stages.Unlike those in wild loquat,the key DEGs and DAMs involved in carbohydrate metabolism,plant hormone signal transduction,f lavonoid biosynthesis,and carotenoid biosynthesis were significantly regulated in cultivated loquats during fruit development.These high-quality reference genome,resequencing,and large-scale transcriptome/metabolome data provide valuable resources for elucidating fruit domestication and molecular breeding in loquat.
文摘Few reports have been seen on the segregation of polyploids and on the relationship between the segregation of SSR loci and that of the morphological traits in polyploid progeny. This paper attempted to gain an insight into the segregation of the hexaploid tobacco progeny and to understand the correlation of the segregation of SSR loci with that of the flower morphological traits. The segregation was evaluated with both SSR markers and morphological traits of the flowers. Twenty pairs of SSR primers were screened and a total of 42 SSR loci were identified. Chi-square analysis showed that the segregation ratio of the SSR loci in the aneuploid progeny was between 1:1 and 2:1. Six morphological traits of flowers, including the lengths of the flower and the calyx, the widths of the calyx, the corolla tube and the corolla, and the coloring of the flower, from 180 progeny and 2 progenitor plants, were determined and Chi-square analyzed. All traits were in consistent also with a segregation ratio of 1:1 to 2:1 except the width of the calyx.