Viral pneumonia has recently been defined as a risk factor for invasive pulmonary aspergillosis(IPA).Wauters et al[1]reported that 23%of critically ill patients who were admitted to the intensive care unit(ICU)with sw...Viral pneumonia has recently been defined as a risk factor for invasive pulmonary aspergillosis(IPA).Wauters et al[1]reported that 23%of critically ill patients who were admitted to the intensive care unit(ICU)with swine-origin influenza A(H1N1)viral infection had developed invasive aspergillosis.IPA was diagnosed in 83 of 432(19%)patients who were admitted with influenza;for patients with influenza who were immunocompromised,the incidence of IPA reached up to 32%(38 of 117 patients).展开更多
Cellular uptake of biomolecules is crucial for regulating cell function.However,powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs(ssDNAs)remain elusive.Herein,we cons...Cellular uptake of biomolecules is crucial for regulating cell function.However,powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs(ssDNAs)remain elusive.Herein,we constructed synthetic DNA circuits on the cell membrane to program the cell entry of ssDNAs,using toehold-mediated DNA strand displacement reactions.We found that the dimerization and trimerization of cholesterol-ssDNAs enhanced membrane-anchoring and cellular uptake of ssDNAs.Moreover,we demonstrated that de-dimerization and de-trimerization of cholesterol-ssDNAs could be accomplished by inputting recovery ssDNAs into the synthetic DNA circuits,which could simultaneously decrease the cellular uptake of ssDNAs.We speculate that operating the synthetic DNA circuits on the cell membrane will be a powerful strategy for regulating the cellular uptake of exogenous materials,which has important implications for bioimaging,drug delivery,and gene therapy.展开更多
基金China National Key Research and Development Program(No.2018YFE0102100)
文摘Viral pneumonia has recently been defined as a risk factor for invasive pulmonary aspergillosis(IPA).Wauters et al[1]reported that 23%of critically ill patients who were admitted to the intensive care unit(ICU)with swine-origin influenza A(H1N1)viral infection had developed invasive aspergillosis.IPA was diagnosed in 83 of 432(19%)patients who were admitted with influenza;for patients with influenza who were immunocompromised,the incidence of IPA reached up to 32%(38 of 117 patients).
基金supported by the National Natural Science Foundation of China(Grants No.22025404,21904086).
文摘Cellular uptake of biomolecules is crucial for regulating cell function.However,powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs(ssDNAs)remain elusive.Herein,we constructed synthetic DNA circuits on the cell membrane to program the cell entry of ssDNAs,using toehold-mediated DNA strand displacement reactions.We found that the dimerization and trimerization of cholesterol-ssDNAs enhanced membrane-anchoring and cellular uptake of ssDNAs.Moreover,we demonstrated that de-dimerization and de-trimerization of cholesterol-ssDNAs could be accomplished by inputting recovery ssDNAs into the synthetic DNA circuits,which could simultaneously decrease the cellular uptake of ssDNAs.We speculate that operating the synthetic DNA circuits on the cell membrane will be a powerful strategy for regulating the cellular uptake of exogenous materials,which has important implications for bioimaging,drug delivery,and gene therapy.
