Glucosinolates are a group of important sulfur and nitrogen containing plant secondary metabolites found in Brassicaceous vegetables that are associated with many health benefits.There is a growing interest in assessi...Glucosinolates are a group of important sulfur and nitrogen containing plant secondary metabolites found in Brassicaceous vegetables that are associated with many health benefits.There is a growing interest in assessing dietary glucosinolates and their degradation products’isothiocyanates exposure to humans.Determination of glucosinolates in plants is a very challenging task.In this study,the total glucosinolates from 22 commonly consumed Brassicaceous vegetables in the US market were determined by employing 1,2-benzenedithiol cyclocondensation for the determination of isothiocyanates(ITCs)from non-indole glucosinolates and a colorimetric method for the determination of thiocyanate ion(SCN^(−))from indole glucosi-nolates.The total glucosinolates were determined from the sum of these two methods.In the 22 investigated vegetables,the highest level of total ITCs was found in horse radish(5189.0±544.8 nmol/g dry weight)and the highest level of total SCN^(−)level was found in brussel sprouts(1294.0±141.6 nmol/g dry weight).The total glucosinolates ranged from 18.6±3.2 to 2271.2±215.3 nmol/g dry weight.The total glucosinolate and ITC data are necessary for the USDA glucosinolates database and important for establishing the relationship of glucosinolates to human nutrition.展开更多
Lettuce(Lactuca sativa L.)is one of the most consumed vegetables in the world and different management practice can result in considerable variability of the secondary metabolites.Flow injection mass spectrometry(FIMS...Lettuce(Lactuca sativa L.)is one of the most consumed vegetables in the world and different management practice can result in considerable variability of the secondary metabolites.Flow injection mass spectrometry(FIMS)combined with analysis of variance-principle component analysis(ANOVA-PCA)was used to study differences in the secondary metabolites originat-ing from different lighting conditions(Sunlight,white light,and florescent light)and lettuce varieties(Romaine and Lollo Rossa).Ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry was used for putative marker compound identification.Quinic acid,caffeic acid,chlorogenic acid,L-chicoric acid,and quercetin malonyl gluco-side varied significantly for Romaine lettuce grown under different light conditions.The study showed that the combination of FIMS fingerprinting and ANOVA-PCA can be a useful tool for the characterization of the sources of variance in plant materials regarding to genetic,environmental,and management factors.展开更多
基金This research is supported by the Agricultural Research Service of the US Department of Agriculture,an Interagency Agreement with the Office of Dietary Supplements at the National Institutes of Health.
文摘Glucosinolates are a group of important sulfur and nitrogen containing plant secondary metabolites found in Brassicaceous vegetables that are associated with many health benefits.There is a growing interest in assessing dietary glucosinolates and their degradation products’isothiocyanates exposure to humans.Determination of glucosinolates in plants is a very challenging task.In this study,the total glucosinolates from 22 commonly consumed Brassicaceous vegetables in the US market were determined by employing 1,2-benzenedithiol cyclocondensation for the determination of isothiocyanates(ITCs)from non-indole glucosinolates and a colorimetric method for the determination of thiocyanate ion(SCN^(−))from indole glucosi-nolates.The total glucosinolates were determined from the sum of these two methods.In the 22 investigated vegetables,the highest level of total ITCs was found in horse radish(5189.0±544.8 nmol/g dry weight)and the highest level of total SCN^(−)level was found in brussel sprouts(1294.0±141.6 nmol/g dry weight).The total glucosinolates ranged from 18.6±3.2 to 2271.2±215.3 nmol/g dry weight.The total glucosinolate and ITC data are necessary for the USDA glucosinolates database and important for establishing the relationship of glucosinolates to human nutrition.
文摘Lettuce(Lactuca sativa L.)is one of the most consumed vegetables in the world and different management practice can result in considerable variability of the secondary metabolites.Flow injection mass spectrometry(FIMS)combined with analysis of variance-principle component analysis(ANOVA-PCA)was used to study differences in the secondary metabolites originat-ing from different lighting conditions(Sunlight,white light,and florescent light)and lettuce varieties(Romaine and Lollo Rossa).Ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry was used for putative marker compound identification.Quinic acid,caffeic acid,chlorogenic acid,L-chicoric acid,and quercetin malonyl gluco-side varied significantly for Romaine lettuce grown under different light conditions.The study showed that the combination of FIMS fingerprinting and ANOVA-PCA can be a useful tool for the characterization of the sources of variance in plant materials regarding to genetic,environmental,and management factors.
