A Novel Method for the Reconstruction of Road Profiles from Measured Vehicle Responses Based on the Kalman Filter Method

The estimation of the disturbance input acting on a vehicle from its given responses is an inverse problem.To overcome some of the issues related to ill-posed inverse problems,this work proposes a method of reconstructing the road roughness based on the Kalman filter method.A half-car model that considers both the vehicle and equipment is established,and the joint input-state estimation method is used to identify the road profile.The capabilities of this methodology in the presence of noise are numerically demonstrated.Moreover,to reduce the influence of the driving speed on the estimation results,a method of choosing the calculation frequency is proposed.A road vibration test is conducted to benchmark the proposed method.

Water takeoff performance calculation method for amphibious aircraft based on digital virtual flight

Owing to the strong coupling among the hydrodynamic forces,aerodynamic forces and motion of amphibious aircraft during the water takeoff process,the water takeoff performance is difficult to calculate accurately and quickly.Based on an analysis of the dynamics and kinematics characteristics of amphibious aircraft and the hydrodynamic theory of high-speed planing hulls,a suitable mathematical model is established for calculating the hydrodynamics of aircraft during water takeoff.A pilot model is designed to illustrate how pilots are affected by the lack of visual reference and the necessity to simultaneously control the pitch angle,flight velocity and other parameters during water takeoff.Combined with the aerodynamic model,engine thrust model and aircraft motion model,a digital virtual flight simulation model is developed for amphibious aircraft during water takeoff,and a calculation method for the water takeoff performance of amphibious aircraft is proposed based on digital virtual flight.Typical performance indicators,such as the liftoff time and liftoff distance,can be obtained via digital virtual flight calculations.A comparison of the measured flight test data and the calculation results shows that the calculation error is less than 10%,which verifies the correctness and accuracy of the proposed method.This method can be used for the preliminary evaluation of airworthiness compliance of amphibious aircraft design schemes,and the relevant calculation results can also provide a theoretical reference for the formulation of flight test plans for airworthiness certification.

Recent advances in RNA structurome

RNA structures are essential to support RNA functions and regulation in various biological processes. Recently, a range of novel technologies have been developed to decode genome-wide RNA structures and novel modes of functionality across a wide range of species. In this review, we summarize key strategies for probing the RNA structurome and discuss the pros and cons of representative technologies. In particular, these new technologies have been applied to dissect the structural landscape of the SARS-CoV-2 RNA genome. We also summarize the functionalities of RNA structures discovered in different regulatory layers-including RNA processing, transport, localization, and mRNA translation-across viruses, bacteria, animals, and plants. We review many versatile RNA structural elements in the context of different physiological and pathological processes(e.g., cell differentiation, stress response, and viral replication). Finally, we discuss future prospects for RNA structural studies to map the RNA structurome at higher resolution and at the single-molecule and single-cell level, and to decipher novel modes of RNA structures and functions for innovative applications.

Three-axis coupled flight control law design forflying wing aircraft using eigenstructure assignment method

Due to elimination of horizontal and vertical tails,flying wing aircraft has poor longitudinal and directional dynamic characteristics.In addition,flying wing aircraft uses drag rudders for yaw control,which tends to generate strong three-axis control coupling.To overcome these problems,a flight control law design method that couples the longitudinal axis with the lateraldirectional axes is proposed.First,the three-axis coupled control augmentation structure is specified.In the structure,a‘‘soft/hard"cross-connection method is developed for three-axis dynamic decoupling and longitudinal control response decoupling from the drag rudders;maneuvering turn angular rate estimation and subtraction are used in the yaw axis to improve the directional damping.Besides,feedforward control is adopted to improve the maneuverability and control decoupling performance.Then,detailed design methods for feedback and feedforward control parameters are established using eigenstructure assignment and model following technique.Finally,the proposed design method is evaluated and compared with conventional method by numeric simulations.The influences of control derivatives variation of drag rudders on the method are also analyzed.It is demonstrated that the method can effectively improve the dynamic characteristics of flying wing aircraft,especially the directional damping characteristics,and decouple the longitudinal responses from the drag rudders.

An ultra low-input method for global RNA structure probing uncovers Regnase-1-mediated regulation in macrophages

To enable diverse functions and precise regulation,an RNA sequence often folds into complex yet distinct structures in different cellular states.Probing RNA in its native environment is essential to uncovering RNA structures of biological contexts.However,current methods generally require large amounts of input RNA and are challenging for physiologically relevant use.Here,we report smartSHAPE,a new RNA structure probing method that requires very low amounts of RNA input due to the largely reduced artefact of probing signals and increased efficiency of library construction.Using smartSHAPE,we showcased the profiling of the RNA structure landscape of mouse intestinal macrophages upon inflammation,and provided evidence that RNA conformational changes regulate immune responses.These results demonstrate that smartSHAPE can greatly expand the scope of RNA structure-based investigations in practical biological systems,and also provide a research paradigm for the study of post-transcriptional regulation.