Background: To evaluate the short-term and long-term outcomes after laparoscopic hysterectomy (LH) compared with abdominal hysterectomy (AH) in case of benign gynecological disease. Methods: A multi-center cohor...Background: To evaluate the short-term and long-term outcomes after laparoscopic hysterectomy (LH) compared with abdominal hysterectomy (AH) in case of benign gynecological disease. Methods: A multi-center cohort retrospective comparative study of population among 4,895 hysterectomies (3,539 LH vs.1,356 AH) between 2007 and 2013 was involved. Operative time (OT), estimated blood loss (EBL), intra-operative and post-operative complications, passing flatus; days with indwelling catheter, questionnaires covering pelvic floor functions and sexual functions were assessed. Results: The EBL (174.1±157.4 vs. 263.1±183.2 cc, LH and AH groups, respectively), passing flatus (38.7±14.1 vs. 48.1±13.2 hours), days with indwelling catheter (1.5±0.6 vs. 2.2±0.8 days), use of analgesics (6.5% vs. 73.1%), intra-operative complication rate (2.4% vs. 4.1%), post-operative complication rate (2.3 % vs. 5.7%), post-operative constipation (12.1% vs. 24.6%), mild and serious stress urinary incontinence (SUI) post-operative (P〈0.001; P=0.014), and proportion of Female Sexual Functioning Index (FSFI) total score 〈26.55 post-operative (P〈0.001) of the LH group were significantly less than those of AH group. There were no significant differences in OT (106.5±34.5 vs. 106.2±40.3 min) between the two groups. Conclusions: LH is a safe and efficient operation for improving patients' long-term quality of life (QoL), and LH is a cost-effectiveness procedure for treating benign gynecological disease. LH is superior to AH due to reduced EBL, reduced post-operative pain and earlier passing flatus.展开更多
Objective:To investigate the effects and mechanisms of aspirin on the proliferation,migration and invasion of endometrial carcinoma HEC-1 A cell lines.Methods:HEC-1 Acells were cultured to the exponential phase and tr...Objective:To investigate the effects and mechanisms of aspirin on the proliferation,migration and invasion of endometrial carcinoma HEC-1 A cell lines.Methods:HEC-1 Acells were cultured to the exponential phase and treated with different concentrations of aspirin(0.625 mmol/L,1.25 mmol/L,2.5 mmol/L,5 mmol/L and 10 mmol/L)for 24 to 120 hours.Cell proliferation was assessed by methyl thiazolyl tetrazolium(MTT)assay.The migration and invasion of HEC-1 Acells were detected by transwell assay.The protein expressions of vascular endothelial growth factor(VEGF)and ascular endothelial growth factor receptor 2(VEGFR-2)in HEC-1 Acells were determined by western blotting.Results:MTT results showed that aspirin inhibited the growth and proliferation of endometrial cancer cells in concentration and time-dependent manner.Aspirin had a significant inhibitory effect on the migration and invasion of HEC-1 Acells(P<0.05).In addition,aspirin obviously suppressed concentration-dependently the expression levels of VEGF and VEGFR-2(P<0.05).Conclusion:Aspirin could inhibit the proliferation,migration and invasion of endometrial cancer cells.The anti-tumor mechanism of aspirin might be related to the inhibition of tumor angiogenesis via blocking the VEGF/VEGFR-2 signaling pathway.展开更多
Objective:To construct a non-replicating vaccinia virus expressing human papillomavirus 16(HPV16) L1, L2E7 proteins as a candidate vaccine for cervical cancer.Methods:Using vaccinia virus vector, we generated a strain...Objective:To construct a non-replicating vaccinia virus expressing human papillomavirus 16(HPV16) L1, L2E7 proteins as a candidate vaccine for cervical cancer.Methods:Using vaccinia virus vector, we generated a strain of non-replicating recombinant vaccinia virus vaccine expressing HPV16 L1, L2E7 proteins by homologous recombination and identified by PCR and Western-bloting.Results:We demonstrated that the L1, L2E7 gene of HPV16 were integrated into vaccinia genosome and could express L1, L2E7 protein stably when infected the CEF using PCR and Western-blot assay.Conclusion:NTVJL1/L2E7 can express L1, L2E7 protein of HPV16 and can be taken as a candidate vaccine for HPV16-associated diseases.展开更多
High dielectric loss and poor temperature stability are the current barriers to the application of dielectric materials.In present work,we synthesized a system of acceptor Gd^(3+)and donor Ta^(5+)co-doped(Gd_(0.5)Ta_(...High dielectric loss and poor temperature stability are the current barriers to the application of dielectric materials.In present work,we synthesized a system of acceptor Gd^(3+)and donor Ta^(5+)co-doped(Gd_(0.5)Ta_(0.5))_(x)Ti_(1-x)O_(2)(GTTOx,x=0,0.01,0.02,0.04,0.06)ceramics to enhance dielectric response.It was found that a colossal permittivity(CP,2.65×10^(4)@1 kHz,2.37×10^(4)@1 MHz),a very low dielectric loss(tanδ,0.007@1 kHz,0.03@1 MHz),good stability of frequency(20–10^(6)Hz)and temperature(RT–250°C,Δε′(T)/ε′30<±15%,at 1 kHz)were achieved simultaneously in GTTO0.01 ceramic.Complex impedance spectroscopy,XPS,SEM,and Raman spectroscopy were used to investigate the reasons for the improved dielectric properties.The result indicated that the main reasons for CP and low dielectric loss are the synergistic effect of the electron pinning defect-dipole(EPDD)model,the internal blocking layer capacitance(IBLC)mechanism,and electrode response.This work provides a promising approach for the design of defect-related high-performance giant dielectric ceramics.展开更多
A habitat suitability index model(HSI)was established to examine seasonal habitat pattern of Sthenoteuthis oualaniensis in the South China Sea and assessed the impacts of the El Niño event on habitat variations b...A habitat suitability index model(HSI)was established to examine seasonal habitat pattern of Sthenoteuthis oualaniensis in the South China Sea and assessed the impacts of the El Niño event on habitat variations based on sea surface temperature(SST),chlorophyll-a(Chla)and sea surface height(SSH).Environmental conditions and habitat patterns showed significant seasonal and spatial variations.Spatial pattern of favorable environmental conditions played important roles in regulating the ranges and longitudinal and latitudinal distributions of suitable habitats(areas with HSI≥0.6)of S.oualaniensis across seasons.Significant positive correlation was found between SST and HSI,whereas negative relationship was revealed between HSI and Chla as well as SSH by correlation analysis.Comparing to normal climate condition in spring 2014,the El Niño events in spring 2015 and 2016 yielded lower SST and higher Chla and SSH,which were unfavorable for the formation of high-quality habitats.Favorable ranges of environmental conditions showed decreasing trend from spring 2014 to 2016.Consequently,the suitable habitats in spring 2015 and 2016 largely contracted and became discontinuity.Our findings indicated that seasonal habitat patterns of S.oualaniensis in the South China Sea were strongly affected by the El Niño events and local environmental conditions.展开更多
Pelagic cephalopods have evolved a series of fascinating traits,such as excellent visual acuity,high-speed agility,and photophores for adaptation to open pelagic oceans.However,the genetic mechanisms underpinning thes...Pelagic cephalopods have evolved a series of fascinating traits,such as excellent visual acuity,high-speed agility,and photophores for adaptation to open pelagic oceans.However,the genetic mechanisms underpinning these traits are not well understood.Thus,in this study,we obtained high-quality genomes of two purpleback flying squid species(Sthenoteuthis oualaniensis and Sthenoteuthis sp.),with sizes of 5450 Mb and 5651 Mb,respectively.Comparative genomic analyses revealed that the S-crystallin subfamily SL20-1 associated with visual acuity in the purpleback flying squid lineage was significantly expanded,and the evolution of high-speed agility for the species was accompanied by significant positive selection pressure on genes related to energy metabolism.These molecular signals might have contributed to the evolution of their adaptative predatory and anti-predatory traits.In addition,the transcriptomic analysis provided clear indications of the evolution of the photophores of purpleback flying squids,especially the recruitment of new genes and energy metabolism-related genes which may have played key functional roles in the process.展开更多
基金supported by the National Natural Science Foundation of China(No.81360388)Guangxi Natural Science Foundation (No.2016 GXNSFAA380258)+1 种基金Self-financing Project of Guangxi Health and Family Planning Commission(No.z2016282)Guangxi Graduate Education Innovation Program (No.YCSW2017104)
基金supported be Self-financing Research Project of Health and Family Planning commission in Guangxi Zhuang Autonomous Region (project number: Z2015626)Scientific Research and Technology Development Plan of Liuzhou Science and Technology Agency in Guangxi (project number: 2015J030508)
文摘Background: To evaluate the short-term and long-term outcomes after laparoscopic hysterectomy (LH) compared with abdominal hysterectomy (AH) in case of benign gynecological disease. Methods: A multi-center cohort retrospective comparative study of population among 4,895 hysterectomies (3,539 LH vs.1,356 AH) between 2007 and 2013 was involved. Operative time (OT), estimated blood loss (EBL), intra-operative and post-operative complications, passing flatus; days with indwelling catheter, questionnaires covering pelvic floor functions and sexual functions were assessed. Results: The EBL (174.1±157.4 vs. 263.1±183.2 cc, LH and AH groups, respectively), passing flatus (38.7±14.1 vs. 48.1±13.2 hours), days with indwelling catheter (1.5±0.6 vs. 2.2±0.8 days), use of analgesics (6.5% vs. 73.1%), intra-operative complication rate (2.4% vs. 4.1%), post-operative complication rate (2.3 % vs. 5.7%), post-operative constipation (12.1% vs. 24.6%), mild and serious stress urinary incontinence (SUI) post-operative (P〈0.001; P=0.014), and proportion of Female Sexual Functioning Index (FSFI) total score 〈26.55 post-operative (P〈0.001) of the LH group were significantly less than those of AH group. There were no significant differences in OT (106.5±34.5 vs. 106.2±40.3 min) between the two groups. Conclusions: LH is a safe and efficient operation for improving patients' long-term quality of life (QoL), and LH is a cost-effectiveness procedure for treating benign gynecological disease. LH is superior to AH due to reduced EBL, reduced post-operative pain and earlier passing flatus.
基金supported by The Natural Science Fund of Guangxi Province (No. 2016GXNSFAA380258)Innovation Project of Guangxi Graduate Education(No.YCSW2017104)The Health and Family Planning Commission's Self-financed Scientific Research Project of Guangxi (No.Z2016282)
文摘Objective:To investigate the effects and mechanisms of aspirin on the proliferation,migration and invasion of endometrial carcinoma HEC-1 A cell lines.Methods:HEC-1 Acells were cultured to the exponential phase and treated with different concentrations of aspirin(0.625 mmol/L,1.25 mmol/L,2.5 mmol/L,5 mmol/L and 10 mmol/L)for 24 to 120 hours.Cell proliferation was assessed by methyl thiazolyl tetrazolium(MTT)assay.The migration and invasion of HEC-1 Acells were detected by transwell assay.The protein expressions of vascular endothelial growth factor(VEGF)and ascular endothelial growth factor receptor 2(VEGFR-2)in HEC-1 Acells were determined by western blotting.Results:MTT results showed that aspirin inhibited the growth and proliferation of endometrial cancer cells in concentration and time-dependent manner.Aspirin had a significant inhibitory effect on the migration and invasion of HEC-1 Acells(P<0.05).In addition,aspirin obviously suppressed concentration-dependently the expression levels of VEGF and VEGFR-2(P<0.05).Conclusion:Aspirin could inhibit the proliferation,migration and invasion of endometrial cancer cells.The anti-tumor mechanism of aspirin might be related to the inhibition of tumor angiogenesis via blocking the VEGF/VEGFR-2 signaling pathway.
基金Support by a grant from the National High Technology Research and Development Program, China (No.2002AA216041).
文摘Objective:To construct a non-replicating vaccinia virus expressing human papillomavirus 16(HPV16) L1, L2E7 proteins as a candidate vaccine for cervical cancer.Methods:Using vaccinia virus vector, we generated a strain of non-replicating recombinant vaccinia virus vaccine expressing HPV16 L1, L2E7 proteins by homologous recombination and identified by PCR and Western-bloting.Results:We demonstrated that the L1, L2E7 gene of HPV16 were integrated into vaccinia genosome and could express L1, L2E7 protein stably when infected the CEF using PCR and Western-blot assay.Conclusion:NTVJL1/L2E7 can express L1, L2E7 protein of HPV16 and can be taken as a candidate vaccine for HPV16-associated diseases.
基金The work was supported by the National Natural Science Foundation of China(No.U21A2074,52072381)Tianjin Science and technology plan Program(Grant No.19ZYPTJC00070).
文摘High dielectric loss and poor temperature stability are the current barriers to the application of dielectric materials.In present work,we synthesized a system of acceptor Gd^(3+)and donor Ta^(5+)co-doped(Gd_(0.5)Ta_(0.5))_(x)Ti_(1-x)O_(2)(GTTOx,x=0,0.01,0.02,0.04,0.06)ceramics to enhance dielectric response.It was found that a colossal permittivity(CP,2.65×10^(4)@1 kHz,2.37×10^(4)@1 MHz),a very low dielectric loss(tanδ,0.007@1 kHz,0.03@1 MHz),good stability of frequency(20–10^(6)Hz)and temperature(RT–250°C,Δε′(T)/ε′30<±15%,at 1 kHz)were achieved simultaneously in GTTO0.01 ceramic.Complex impedance spectroscopy,XPS,SEM,and Raman spectroscopy were used to investigate the reasons for the improved dielectric properties.The result indicated that the main reasons for CP and low dielectric loss are the synergistic effect of the electron pinning defect-dipole(EPDD)model,the internal blocking layer capacitance(IBLC)mechanism,and electrode response.This work provides a promising approach for the design of defect-related high-performance giant dielectric ceramics.
基金This study was financially supported by the Major Projects of Basic and Applied Basic Research Programs in Guangdong Province(2019B030302004)Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(GML2019ZD0605)+1 种基金Key research and development project of Guangdong Province(2020B1111030001)Central Public-Interest Scientific Institution Basal Research Fund(2020TD05 and 2021 SD01).
文摘A habitat suitability index model(HSI)was established to examine seasonal habitat pattern of Sthenoteuthis oualaniensis in the South China Sea and assessed the impacts of the El Niño event on habitat variations based on sea surface temperature(SST),chlorophyll-a(Chla)and sea surface height(SSH).Environmental conditions and habitat patterns showed significant seasonal and spatial variations.Spatial pattern of favorable environmental conditions played important roles in regulating the ranges and longitudinal and latitudinal distributions of suitable habitats(areas with HSI≥0.6)of S.oualaniensis across seasons.Significant positive correlation was found between SST and HSI,whereas negative relationship was revealed between HSI and Chla as well as SSH by correlation analysis.Comparing to normal climate condition in spring 2014,the El Niño events in spring 2015 and 2016 yielded lower SST and higher Chla and SSH,which were unfavorable for the formation of high-quality habitats.Favorable ranges of environmental conditions showed decreasing trend from spring 2014 to 2016.Consequently,the suitable habitats in spring 2015 and 2016 largely contracted and became discontinuity.Our findings indicated that seasonal habitat patterns of S.oualaniensis in the South China Sea were strongly affected by the El Niño events and local environmental conditions.
基金supported by grants from the Guangdong Major Project of Basic and Applied Basic Research, China (Grant No. 2019B030302004)the National Key R&D Program of China (Grant No. 2018YFC1406502)+6 种基金the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou),China(Grant No. GML2019ZD0605)the Financial Fund of the Ministry of Agriculture and Rural Affairs of China (Grant No. NFZX2018)the Central Public-interest Scientific Basal Research Fund, Chinese Academy of Fishery Sciences (Grant Nos. 2019HY-XKQ03, 2020TD05, and 2021SD18)the China Postdoctoral Science Foundation (Grant No. 2021M693342)the Hubei Postdoctoral Innovation Post Project, Chinathe 1000 Talent Project of Shaanxi Province, Chinathe Research Funds for Interdisciplinary subject of Northwestern Polytechnical University, China (Grant No. 19SH030408)
文摘Pelagic cephalopods have evolved a series of fascinating traits,such as excellent visual acuity,high-speed agility,and photophores for adaptation to open pelagic oceans.However,the genetic mechanisms underpinning these traits are not well understood.Thus,in this study,we obtained high-quality genomes of two purpleback flying squid species(Sthenoteuthis oualaniensis and Sthenoteuthis sp.),with sizes of 5450 Mb and 5651 Mb,respectively.Comparative genomic analyses revealed that the S-crystallin subfamily SL20-1 associated with visual acuity in the purpleback flying squid lineage was significantly expanded,and the evolution of high-speed agility for the species was accompanied by significant positive selection pressure on genes related to energy metabolism.These molecular signals might have contributed to the evolution of their adaptative predatory and anti-predatory traits.In addition,the transcriptomic analysis provided clear indications of the evolution of the photophores of purpleback flying squids,especially the recruitment of new genes and energy metabolism-related genes which may have played key functional roles in the process.