Effects of cardiac rehabilitation qigong exercise in patients with stable coronary artery disease undergoing phase Ⅲ rehabilitation: A randomized controlled trial (with video)

Objective:To explore the effectiveness and safety of cardiac rehabilitation qigong exercise in stable coronary artery disease(CAD)patients undergoing phaseⅢrehabilitation.Methods:This was a randomized controlled trial.A total of 59 stable CAD patients undergoing phaseⅢrehabilitation treated in Sports Medicine Hospital from March 2017 to September 2017 were enrolled after meeting the inclusion criteria and then divided into cardiac rehabilitation qigong exercise group(n=30)receiving a 12-week intervention and control group(n=29).All participants were assessed at baseline and at 12-week intervention for the primary outcome,that was treadmill test parameter and the secondary outcomes including physical fitness,body composition,bone mineral density,and cardiac ultrasound B-mode imaging.Results:There were no significant differences in baseline demographics between the two groups.After a 12-week cardiac rehabilitation qigong exercise intervention,compared with the control group,ΔV02(initial 1352.63±340.95 vs 12 weeks 1594.57±467.14)vs(initial 1363.83±322.90 vs 12 weeks 1323.76±318.92)(P=.003),ΔVO2/kg(initial 21.23±3.56 vs 12 weeks 24.75±5.11)vs(initial 21.01±3.71 vs 12 weeks 20.35±3.66)(P=.002),ΔMETS(initial 6.19±1.12 vs 12 weeks 7.16±1.60)vs(initial 6.00±1.19 vs 12 weeks 5.86±1.23)(P=.001),ΔVO2/HR(P=.027),ΔSV(P=.014),ΔOUES(P=.012),Δhand-grip strength(P=.002),Δflexibility(P=.001),Δbalance(P=.002),ΔT-score(P=.042),ΔBQI(P=.018).However,Δresting systolic blood pressure(P=.004)andΔresting diastolic pressure(P=.012)decreased in the cardiac rehabilitation qigong exercise group.Conclusion:Cardiac rehabilitation qigong exercise can improve cardiopulmonary aerobic capacity,physical fitness,bone mineral density in patients with stable CAD,suggesting that certain effect and safety for stable CAD patients undergoing phaseⅢrehabilitation can be obtained.

PVF-DA: Privacy-Preserving, Verifiable and FaultTolerant Data Aggregation in MEC

As an emergent-architecture, mobile edge computing shifts cloud service to the edge of networks. It can satisfy several desirable characteristics for Io T systems. To reduce communication pressure from Io T devices, data aggregation is a good candidate. However, data processing in MEC may suffer from many challenges, such as unverifiability of aggregated data, privacy-violation and fault-tolerance. To address these challenges, we propose PVF-DA: privacy-preserving, verifiable and fault-tolerant data aggregation in MEC based on aggregator-oblivious encryption and zero-knowledge-proof. The proposed scheme can not only provide privacy protection of the reported data, but also resist the collusion between MEC server and corrupted Io T devices. Furthermore, the proposed scheme has two outstanding features: verifiability and strong fault-tolerance. Verifiability can make Io T device to verify whether the reported sensing data is correctly aggregated. Strong fault-tolerance makes the aggregator to compute an aggregate even if one or several Io Ts fail to report their data. Finally, the detailed security proofs are shown that the proposed scheme can achieve security and privacy-preservation properties in MEC.

PUMTD:Privacy-Preserving User-Profile Matching Protocol in Social Networks

User profile matching can establish social relationships between different users in the social network.If the user profile is matched in plaintext,the user's privacy might face a security challenge.Although there exist some schemes realizing privacypreserving user profile matching,the resource-limited users or social service providers in these schemes need to take higher computational complexity to ensure the privacy or matching of the data.To overcome the problems,a novel privacy-preserving user profile matching protocol in social networks is proposed by using t-out-of n servers and the bloom filter technique,in which the computational complexity of a user is reduced by applying the Chinese Remainder Theorem,the matching users can be found with the help of any t matching servers,and the privacy of the user profile is not compromised.Furthermore,if at most t-1 servers are allowed to collude,our scheme can still fulfill user profile privacy and user query privacy.Finally,the performance of the proposed scheme is compared with the other two schemes,and the results show that our scheme is superior to them.

ESTABLISHMENT AND VERIFICATION OF MATHEMATICAL MODEL FOR CURRENT LEAKAGE IN BIPOLAR MULTI－COMPARTMENT CELL

ＥＳＴＡＢＬＩＳＨＭＥＮＴＡＮＤＶＥＲＩＦＩＣＡＴＩＯＮＯＦＭＡＴＨＥＭＡＴＩＣＡＬＭＯＤＥＬＦＯＲＣＵＲＲＥＮＴＬＥＡＫＡＧＥＩＮＢＩＰＯＬＡＲＭＵＬＴＩ－ＣＯＭＰＡＲＴＭＥＮＴＣＥＬＬＹａｎｇ；ＪｉａｎｈｏｎｇＺｈａｎｇ；ＱｉｎｇｘｉａｎｇＷａｎ...

Meso-level simulation of gas hydrate dissociation in low-permeability sediments

This paper presents a meso-level simulation of gas hydrate dissociation in low-permeability marine sediments. Interstitial pores are defined to describe fluid flow and particle movement. The proposed model couples multiphase fluid flow with particle movement to simulate the thermodynamics of gas hydrate dissociation triggered by sharp temperature rises. Hydrates respond quickly to temperature rise in low-permeability sediments. Dissociation causes pore pressure to rise rapidly to equilibrium then steadily increase above equilibrium pressure. Lower permeability sediment builds up greater excess pore pressure as the dissipation of pore pressure is constrained.

The Issues Facing the Sustainable Development of Rural Tourism and the Path Selection

There is a long way to go for sustainable development of rural tourism.It is necessary to strengthen the planning for training rural tourism talents,and establish sustainable reserve tourism service personnel;innovate upon the promotion mode of rural tourism and open the tourist source market;strengthen the building of characteristic brand of rural tourism,and create sustainable development core of tourism;give play to the role of government in guiding rural tourism,strengthen the optimization of management of rural tourism market environment,and enhance the rural tourism safety;expand the rural tourism industry chain,and strengthen the management planning of sales market of rural tourism product.

Influence on proliferation and inducing capacity of bone mesenchymal stem cells during myocardial differentiation and construction of engineered myocardium-like tissue <i>in vitro</i>

The study is to investigate BMSCs ability to differentiate cardiomyocytes, especially discussed cell generations and 5-Aaz concentration influence on BMSCs capability of proliferation and differentiation into cardiomyocytes during constructing the engineered myocardium-like tissue in vitro. The results have demonstrated that the different concentration of 5-Aza has the influence on proliferation rate of different generations of BMSCs, the second generation BMSCs was superior to the sixth, and tenth generation in proliferation capacity after being induced, and 5-Aza had some influence on proliferation capacity. Rat BMSCs could be differentiated into cardiomyocytes-like cells, which have a good biocompatibility with acellular bovine pericardium, and myocardium-like tissue could be engineered with BMSCs and acellular bovine pericardium in vitro. In conclusion, BMSCs could be induced and differentiated into cardiomyocytes-like cells in vitro. Different generations and different 5-Aza density have influence on the rate of increase of BMSCs, and the engineered myocardium-like tissue could be constructed with BMSCs and acellular bovine pericardium in vitro.

Challenges and Opportunities for Privacy Computing

There’s a lot of value in data.The collection,fusion and mining of multi-source data will release the potential of data and bring into play the value of data.At the same time,due to their respective interests and risks of privacy exposure,there are obstacles in the development of mul­ti-data fusion,and the phenomenon of“isolated data is­land”is common,which hinders the development of data fusion.

Strategies on biosynthesis and production of bioactive compounds in medicinal plants

Medicinal plants are a valuable source of essential medicines and herbal products for healthcare and disease therapy.Compared with chemical synthesis and extraction,the biosynthesis of natural products is a very promising alternative for the successful conservation of medicinal plants,and its rapid development will greatly facilitate the conservation and sustainable utilization of medicinal plants.Here,we summarize the advances in strategies and methods concerning the biosynthesis and production of natural products of medicinal plants.The strategies and methods mainly include genetic engineering,plant cell culture engineering,metabolic engineering,and synthetic biology based on multiple“OMICS”technologies,with paradigms for the biosynthesis of terpenoids and alkaloids.We also highlight the biosynthetic approaches and discuss progress in the production of some valuable natural products,exemplifying compounds such as vindoline(alkaloid),artemisinin and paclitaxel(terpenoids),to illustrate the power of biotechnology in medicinal plants.

仿生细胞膜涂层包覆肿瘤阻断代谢物传输

The metabolite transport inhibition of tumor cells holds promise to achieve anti-tumor efficacy.Herein,we presented an innovative strategy to hinder the delivery of metabolites through the in-situ besieging tumor cells with polyphenolic polymers that strongly adhere to the cytomembrane of tumor cells.Simultaneously,these polymers underwent self-crosslinking under the induction of tumor oxidative stress microenvironment to form an adhesive coating on the surface of the tumor cells.This polyphenol coating effectively obstructed glucose uptake,reducing metabolic products such as lactic acid,glutathione,and adenosine triphosphate,while also causing reactive oxygen species to accumulate in the tumor cells.The investigation of various tumor models,including 2D cells,3D multicellular tumor spheroids,and xenograft tumors,demonstrated that the polyphenolic polymers effectively inhibited the growth of tumor cells by blocking key metabolite transport processes.Moreover,this highly adhesive coating could bind tumor cells to suppress their metastasis and invasion.This work identified polyphenolic polymers as a promising anticancer candidate with a mechanism by impeding the mass transport of tumor cells.

三向网格结构无支撑施工关键技术研究

空间网格结构因其独特的建筑造型,在现代建筑中应用越来越多,随着其下方主体结构和空间网格结构越来越复杂,其结构安装技术也在不断地提升和改造。对于下部支承结构较为复杂的单层三向网格结构,常规采用临时支撑胎架进行原位分块安装或者搭设满堂脚手架高空散装的方式都存在措施量大、成本过高、安拆时间长等问题,而液压整体同步提升、顶升以及滑移等新型施工方式不仅同样存在成本和效率问题,而且还有较高的施工难度。针对上述问题,以深圳市“互联网+”未来科技城地块DY01-04街坊西区下沉广场马鞍形单层三向网格穹顶结构为例,对其无支撑施工关键技术进行研究,以实现更少的临时支撑措施量和更高的施工效率。无支撑施工指采用特定的分块方式,能充分利用上一个吊装单元作为下一个吊装单元的支撑结构,以交错搭接的方式,不设临时支撑完成结构的安装。施工的关键在于所采用的特定分块方式划分出的每一个分块均能够在吊装完成后与前一步安装结构形成稳定的结构体系,这个稳定的结构体系与一次设计成型态相比不会产生过大的位移和应力。基于施工全过程仿真分析和现场实测数据,综合考虑了施工过程的安全性、便利性及经济性,对螺旋式、单向交错式和对称交错式三种无支撑施工方式进行对比研究。结果表明,单层三向网格结构无支撑施工安全可靠,施工完成态结构应力及变形与设计一次成型态接近,结构整体应力较小,且环梁施工过程无稳定性问题,安全储备充足;无支撑螺旋式施工分段长度更短,结构应力及变形整体都优于无支撑交错式施工,无支撑对称交错式施工可显著减少单向施工过程中“跷跷板”效应带来的结构额外应力和变形,因此又优于无支撑单向交错式施工;通过在结构位移和应力模拟值较大处布置变形和应力监测点,对结构无支撑螺旋式施工过程中的变形和应力进行监测,监测结果表明:实际结构变形和应力与MIDAS模拟分析结果在发展变化趋势及数值上总体保持一致,体现了无支撑螺旋式施工方案的合理性。

Effects of high-/low-temperature and high-altitude hypoxic environments on gut microbiota of sports people:A retrospective analysis

As an invisible“endocrine organ”,gut microbiota is widely involved in the regulation of nervous system,endocrine system,circulatory system,and digestive system.It is also closely related to host health and the occurrence of many chronic diseases.Relevant literature shows that high temperature,low temperature,and highaltitude hypoxia may have negative effects on commensal microorganisms.The stimulation of exercise may aggravate this reaction,which is related to the occurrence of exercise-induced fever and gastrointestinal and respiratory diseases.The intervention of probiotics can alleviate the above problems to a certain extent.Therefore,this paper takes exercise in a special environment as the starting point,deeply analyses the intervention effect and potential mechanism of probiotics,and provides the theoretical basis and reference for follow-up research and application of probiotics in sports science.

Natural killer cell-triggered vascular transformation:maternal care before birth?

Natural killer(NK)cells are found in lymphoid and non-lymphoid organs.In addition to important roles in immune surveillance,some NK cells contribute to angiogenesis and circulatory regulation.The uterus of early pregnancy is a non-lymphoid organ enriched in NK cells that are specifically recruited to placental attachment sites.In species with invasive hemochorial placentation,these uterine natural killer(uNK)cells,via secretion of cytokines,chemokines,mucins,enzymes and angiogenic growth factors,contribute to the physiological change of mesometrial endometrium into the unique stromal environment called decidua basalis.In humans,uNK cells have the phenotype CD56^(bright) CD16^(dim) and they appear in great abundance in the late secretory phase of the menstrual cycle and early pregnancy.Gene expression studies indicate that CD56^(bright) CD16^(dim) uterine and circulating cells are functionally distinct.In humans but not mice or other species with post-implantation decidualization,uNK cells may contribute to blastocyst implantation and are of interest as therapeutic targets in female infertility.Histological and genetic studies in mice first identified triggering of the process of gestation spiral arterial modification as a major uNK cell function,achieved via interferon(IFN)-c secretion.During spiral arterial modification,branches from the uterine artery that traverse the endometrium/decidua transiently lose their muscular coat and ability to vasoconstrict.The expression of vascular markers changes from arterial to venous as these vessels dilate and become low-resistance,high-volume channels.Full understanding of the vascular interactions of human uNK cells is difficult to obtain because endometrial time-course studies are not possible in pregnant women.Here we briefly review key information concerning uNK cell functions from studies in rodents,summarize highlights concerning human uNK cells and describe our preliminary studies on development of a humanized,pregnant mouse model for in vivo investigations of human uNK cell functions.

Isolation of Lymphocytes and Their Innate Immune Characterizations from Liver,Intestine,Lung and Uterus

In steady-state conditions, the number and distribution of lymphocyte populations are under homeostatic control. New lymphocytes are continuously produced in primary and secondary lymphoid organs and then achieve immune-competence within different tissues, and they must challenge with resident cells for survival. The first step in the study of tissue lymphoid cells is their isolation in intact and viable form appropriate for establishment of in vitro culture systems. For reasons of simplicity, cell purity, cell yields and various purposes, lymphocytes obtained from different tissues in different labs were subjected to diverse protocols. To fully elucidate the nature of the local immune system as well as to adequately study the innate role of lymphocytes in liver, intestine, lung and uterus, we briefly reviewed the characterization of resident lymphocytes, and additional information on those cells from non-lymphoid tissues by using the recommended operation procedure was also presented.

Leukocyte driven-decidual angiogenesis in early.pregnancy

Successful pregnancy and long-term, post-natal maternal and offspring cardiac, vascular and metabolic health require key maternal cardiovascular adaptations over gestation. Within the pregnant decidualizing uterus, coordinated vascular, immunological and stromal cell changes occur. Considerable attention has been given to the roles of uterine natural killer （uNK） cells in initiating decidual spiral arterial remodeling, a process normally completed by mid-gestation in mice and in humans. However, leukocyte roles in much earlier, region specific, decidual vascular remodeling are now being defined. Interest in immune cell-promoted vascular remodeling is driven by vascular aberrations that are reported in human gestational complications such as infertility, recurrent spontaneous abortion, preeclampsia （PE） and fetal growth restriction. Appropriate maternal cardiovascular responses during pregnancy protect mothers and their children from later cardiovascular disease risk elevation. One of the earliest uterine responses to pregnancy in species with hemochorial placentation is stromal cell decidualization, which creates unique niches for angiogenesis and leukocyte recruitment. In early decidua basalis, the aspect of the implantation site that will cradle the developing placenta and provide the major blood vessels to support mature placental functions, leukocytes are greatly enriched and display specialized properties. UNK cells, the most abundant leukocyte subset in early decidua basalis, have angiogenic abilities and are essential for normal early decidual angiogenesis. The regulation of uNK cells and their roles in determining maternal and progeny cardiovascular health over ore^nancv and PostPartum are discussed.

The Wolfiporia cocos Genome and Transcriptome Shed Light on the Formation of Its Edible and Medicinal Sclerotium

Wolfiporia cocos(F. A. Wolf) has been praised as a food delicacy and medicine for centuries in China. Here, we present the genome and transcriptome of the Chinese strain CGMCC5.78 of W. cocos. High-confidence functional prediction was made for 9277 genes among the 10,908 total predicted gene models in the W. cocos genome. Up to 2838 differentially expressed genes(DEGs)were identified to be related to sclerotial development by comparing the transcriptomes of mycelial and sclerotial tissues. These DEGs are involved in mating processes, differentiation of fruiting body tissues, and metabolic pathways. A number of genes encoding enzymes and regulatory factors related to polysaccharide and triterpenoid production were strikingly regulated. A potential triterpenoid gene cluster including the signature lanosterol synthase(LSS) gene and its modified components were annotated. In addition, five nonribosomal peptide synthase(NRPS)-like gene clusters,eight polyketide synthase(PKS) gene clusters, and 15 terpene gene clusters were discovered in the genome. The differential expression of the velevt family proteins, transcription factors,carbohydrate-active enzymes, and signaling components indicated their essential roles in the regulation of fungal development and secondary metabolism in W. cocos. These genomic and transcriptomic resources will be valuable for further investigations of the molecular mechanisms controlling sclerotial formation and for its improved medicinal applications.

Human dNK cell function is differentially regulated by extrinsic cellular engagement and intrinsic activating receptors in first and second trimester pregnancy

Decidual natural killer （dNK） cells express an array of activation receptors to regulate placental immunity and development during early pregnancy. We investigated the functional character of human dNK cells during the first and second trimester of gestation and the interaction between dNK and trophoblast cells. Although the frequency of CD56＋CD16-dNK among the total CD45＋ leukocytes did not change over this period, the expression of the activating receptors, NKp80 and NKG2D, was greatly upregulated. We observed a significantly higher number of extravillous trophoblast cells in proximity to the dNK cells in the first trimester in comparison with the second trimester decidua. NKG2D expression by first trimester dNK cells was decreased when co-cultured with the HTR-8 trophoblast cell line. In the second trimester, functional markers of dNK activation, i.e., angiogenic factor production （e.g., vascular endothelial growth factor, interleukin-8, interferon-gamma）, remained stable despite an increase in NKp80 or NKG2D surface expression. Furthermore, the degranulation capacity of dNK cells, as assessed by CD107a, was decreased in the second trimester. We suggest that in the first trimester, trophoblast-dNK interactions generate a population of dNK cells with a suppressed activating phenotype. In the second trimester, the loss of trophoblast-dNK interactions led to the inhibition of dNK cell function, although their activating receptor expression was increased. We speculate that during pregnancy, two mechanisms operate to modulate the dNK cell activation：suppression of activating receptor levels in the first trimester by trophoblasts and disengagement of receptor-ligand coupling in the second trimester.

A constrained neural network model for soil liquefaction assessment with global applicability

A constrained back propagation neural network(C-BPNN)model for standard penetration test based soil liquefaction assessment with global applicability is developed,incorporating existing knowledge for liquefaction triggering mechanism and empirical relationships.For its development and validation,a comprehensive liquefaction data set is compiled,covering more than 600 liquefaction sites from 36 earthquakes in 10 countries over 50 years with 13 complete information entries.The C-BPNN model design procedure for liquefaction assessment is established by considering appropriate constraints,input data selection,and computation and calibration procedures.Existing empirical relationships for overburden correction and fines content adjustment are shown to be able to improve the prediction success rate of the neural network model,and are thus adopted as constraints for the C-BPNN model.The effectiveness of the C-BPNN method is validated using the liquefaction data set and compared with that of several liquefaction assessment methods currently adopted in engineering practice.The C-BPNN liquefaction model is shown to have improved prediction accuracy and high global adaptability.

代谢标记策略用于体外和体内革兰氏阳性菌的选择性杀灭

细菌感染,尤其是最常见的革兰氏阳性菌引起的感染,严重威胁着人类的生命健康.抗生素是治疗细菌感染的首选药物,但是它的大量使用造成了耐药性细菌的快速滋生和蔓延.近年来,光热抗菌疗法作为一种不易引起细菌耐药性的技术受到了广泛的关注.然而,非特异性的细菌杀伤和对邻近哺乳动物细胞的毒副作用严重限制了光热抗菌疗法的实际应用.本文开发了一种代谢标记策略用于革兰氏阳性菌的选择性光热杀灭.通过二苯并环辛炔(DBCO)和IR-780碘化物的共价连接,制备了生物正交的光热试剂IR780-DBCO.革兰氏阳性菌或阴性菌经过3-叠氮基-D-丙氨酸预处理,利用无铜点击化学反应实现了IR780-DBCO在金黄色葡萄球菌和耐万古霉素肠球菌等革兰氏阳性菌表面的选择性标记.在15 min的近红外光照射下,接近100%的革兰氏阳性菌被有效地杀灭.此外,该方法在小鼠皮肤伤口感染模型中也能够显著地抑制耐万古霉素肠球菌引起的感染.我们提出的基于代谢标记的特异性光热杀灭革兰氏阳性菌的策略将会促进预临床研究中精准抗菌试剂的研发.