The use of low-coherence light is expected to be one of the effective ways to suppress or even eliminate the laser–plasma instabilities that arise in attempts to achieve inertial confinement fusion.In this paper,a re...The use of low-coherence light is expected to be one of the effective ways to suppress or even eliminate the laser–plasma instabilities that arise in attempts to achieve inertial confinement fusion.In this paper,a review of low-coherence high-power laser drivers and related key techniques is first presented.Work at typical low-coherence laser facilities,including Gekko XII,PHEBUS,Pharos III,and Kanal-2 is described.The many key techniques that are used in the research and development of low-coherence laser drivers are described and analyzed,including low-coherence source generation,amplification,harmonic conversion,and beam smoothing of low-coherence light.Then,recent progress achieved by our group in research on a broadband low-coherence laser driver is presented.During the development of our low-coherence high-power laser facility,we have proposed and implemented many key techniques for working with low-coherence light,including source generation,efficient amplification and propagation,harmonic conversion,beam smoothing,and precise beam control.Based on a series of technological breakthroughs,a kilojoule low-coherence laser driver named Kunwu with a coherence time of only 300 fs has been built,and the first round of physical experiments has been completed.This high-power laser facility provides not only a demonstration and verification platform for key techniques and system integration of a low-coherence laser driver,but also a new type of experimental platform for research into,for example,high-energy-density physics and,in particular,laser–plasma interactions.展开更多
To quantitatively analyze main figure,field,agency and level of sponge city research in China,and clear research focus and hot spot in each year,by using the Full Text Database of Chinese Sci-tech Periodicals and othe...To quantitatively analyze main figure,field,agency and level of sponge city research in China,and clear research focus and hot spot in each year,by using the Full Text Database of Chinese Sci-tech Periodicals and other retrieval tools,the statistics and analysis of 3152 research literatures on sponge city published in domestic academic journals of 2004-2016 are conducted based on bibliometrics. It is found that since the concept of " sponge city" was firstly proposed in 2012,development research of sponge city involves 40 subject fields and is mainly published in 32 kinds of journals,which is dominated by natural science research( 1427 literatures). Researchers are mainly from each college and university,some design institutes and Chinese Academy of Sciences. The research could play certain guidance significance for further research and construction of ecological city construction in China.展开更多
Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the presen...Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.展开更多
Wind turbines are usually designed and operated with fixed startup speed. It could perform startup and shutdown operations repeatedly when the wind fluctuates around the startup speed. The excessive stress induced by ...Wind turbines are usually designed and operated with fixed startup speed. It could perform startup and shutdown operations repeatedly when the wind fluctuates around the startup speed. The excessive stress induced by frequent startup and shutdown could enhance the likelihood of component failure and hence negatively impact the availability of a wind turbine. Startup speed with dead band is proposed in this article to prevent the wind turbine from frequent startup. 22 years wind data from the Cheung Chau wind station in Hong Kong are analyzed to evaluate the reduction in the number of startup and potential loss of wind power production using the proposed approach. Numerical simulation suggests that the number of startup could be reduced by half with trivial reduction in potential wind power generation in most of investigated sites once an appropriate dead band is adopted.展开更多
In order to investigate antifungnl effect of medicinal plant extracts on Botrytis cinerea, the antifungal activity of ethanol extracts of 8 medicinal plants in- eluding Ginkgo biloba against Botrytis cinerea was deter...In order to investigate antifungnl effect of medicinal plant extracts on Botrytis cinerea, the antifungal activity of ethanol extracts of 8 medicinal plants in- eluding Ginkgo biloba against Botrytis cinerea was determined by growth rate method. The results showed that Ginkgo biloba and Ginnamomum cassia extracts had significant inhibition effects on B. cinema mycelium growth （P 〈 0.05）, with inhibition rates over 70% and ECho values of 0.027 mg/ml and 0.039 mg/ml, re- spectively, significantly different from the antifungal effects of other kinds of medicinal plants ; and P. ternata and A. tsaoko extracts showed the second highest in- hibition effects, with ECho values of 0. 011 9 and 0. 039 3 mg/ml, respectively. Further experimental application could be carried out.展开更多
The'Internet+QQ farm'creative agriculture presented in this paper is the innovation based on the currently booming reality QQ farm,aimed at reducing cost,improving efficiency and optimizing human and material ...The'Internet+QQ farm'creative agriculture presented in this paper is the innovation based on the currently booming reality QQ farm,aimed at reducing cost,improving efficiency and optimizing human and material resource elements.It can make Internet provide technical support for intelligence-based life,leisure,entertainment,with good prospects for development.展开更多
Sustainable building in China has gained attention both domestically and abroad. Despite the fast increase in sustainable assessment tools developed locally or adopted from overseas, there are still criticisms about t...Sustainable building in China has gained attention both domestically and abroad. Despite the fast increase in sustainable assessment tools developed locally or adopted from overseas, there are still criticisms about the current situation of weak implementation and lack of comprehensive consideration. The lack of consideration of economic and social aspects or building performance on whole building life cycle all lead to departure from the true meaning of sustainable development. And lack of participation on the part of stakeholders makes it too theoretical to be carried out. This research aims to develop a model to address this problem. This research started with review of current sustainable assessment tools applied in China. As the assessment indicators have clear regional disparities, and almost no current tool considers all three pillars of environmental, economic and social in building life cycle. An industry survey was therefore designed for generation of indicators at different building stages, and personal interviews relevant to different occupation in building industry were conducted to complement the questionnaire survey. After that, the model Building Sustainable Score (BSS) was developed based on the stakeholders’ participation. Finally, the model is verified by a case study.展开更多
Through the personnel training program,revising the syllabus,optimizing teaching contents,reforming teaching methods,strengthening practical teaching links and reforming examination methods,this paper explored the tea...Through the personnel training program,revising the syllabus,optimizing teaching contents,reforming teaching methods,strengthening practical teaching links and reforming examination methods,this paper explored the teaching reform and practice of Plant Growth Environment Course,in order to improve the teaching effect,stimulate learning interests of students,and cultivate application type talents meeting social demands.展开更多
Pesticide packaging wastes pose a serious hazard to the agricultural production environment and the quality of agricultural products. The investigation for 2 consecutive years in Jietou Region of Tengchong County, a m...Pesticide packaging wastes pose a serious hazard to the agricultural production environment and the quality of agricultural products. The investigation for 2 consecutive years in Jietou Region of Tengchong County, a major tobacco planting area in Yunan, showed that： ① More than 80% of the pesticides purchased by farmers were used for tobacco cultivation, which was 4 times that of conventional crops planting, and most of the packaging materials were plastic bags; ② The main way for farmers to dispose plastic bags was to burn or discard them in the field garbage collection baskets; ③ In combination with field surveys, an average of 4.54 pieces of pesticide packaging wastes were scattered around every 667 m^2 of tobacco field. In addition, the number reduced significantly after carrying out pesticide packaging wastes recycling and control project at the local smoke station. At the same time, the average number of pesticide packaging wastes in the field collection baskets increased. Therefore, it can effectively control pesticide packaging wastes by changing the type of pesticide packaging, encouraging the establishment of recycling system and strengthening the cognitive training of farmers.展开更多
Background:Food-borne parasitic diseases decrease food safety and threaten public health.The snail species is an intermediate host for numerous human parasitic trematodes.Orientogalba ollula has been reported as inter...Background:Food-borne parasitic diseases decrease food safety and threaten public health.The snail species is an intermediate host for numerous human parasitic trematodes.Orientogalba ollula has been reported as intermediate hosts of many zoonotic trematodes.Here,we investigated the prevalence of zoonotic trematodes withinO.ollula in Guangxi,China,and assessed their zoonotic potential.Methods:Snails were collected from 54 sites in 9 cities throughout Guangxi.The snail and trematode larvae species were determined by combining morphological characteristics and molecular markers.The trematodes prevalence and constituent ratio were calculated and compared among different habitat environments.Phylogenetic trees of the trematode species were constructed using the neighbor-joining method with nuclear internal transcribed spacer 2(ITS2)sequences.The developmental cycles of the isolated trematodes were examined by experimental infection in ducks.The developmental characteristics ofEchinostoma revolutum was recorded by dissecting infected ducklings from 1-day post infection(dpi)to 10 dpi.Results:The overall prevalence of trematode larvae was 22.1%(1818/8238)inO.ollula from 11 sample sites.Morphological together with molecular identification,showed thatE.revolutum,Australapatemon sp.,Hypoderaeum conoideum,Pharyngostomum cordatum,andEchinostoma sp.parasitizedO.ollula,with the highest infection rate ofE.revolutum(13.0%).However,noFasciola larvae were detected.The trematodes prevalence and constituent ratio varied in two sub-biotypes(P<0.01).A neighbor-joining tree analysis of ITS2 sequences resulted in distinct monophyletic clades supported by sequences from isolated larvae with high bootstrap values.Ducklings exposed toO.ollula infected withEchinostoma sp.,E.revolutum,andH.conoideum larvae were successfully infected.The animal model forEchinostoma revolutum was successfully established.E.revolutum matured from larvae to adult at 10 dpi in the intestine of the duck,and the developmental characteristics ofE.revolutum were characterized by the maturation of the reproductive and digestive organs at 6-8 dpi.Conclusions:This study revealed a high prevalence of zoonotic trematodes inO.ollula from Guangxi,China.Existing trematodes infection in animals and human clinical cases,coupled with the wide geographical distribution ofO.ollula,necessitate further evaluations of the potential risk of spillover of zoonotic infection from animal to human and vice versa.展开更多
To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor(VEGF)and the possible mechanism of VEGF,the in vitro cultured vascular endothelial cells of rabbit aorta were divide...To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor(VEGF)and the possible mechanism of VEGF,the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group,VEGF-treated group and VEGF+N-nitro-L-arginine methyl ester(L-NAME)-treated group.The absorbance(A)value of vascular endothelial cells and the levels of prostaglandin(PGI2),endothelin-1(ET-1)and von Willebrand factor(vWF)in the supernatant were observed by water-soluble tetrazo-lium salt assay,radioimmunoassay and enzyme-linked immunosorbent assay.The A values and PGI2 in VEGF-treated group and VEGF+L-NAME-treated group were higher than those in control group(P<0.05 and P<0.01).The ET-1 and vWF were significantly decreased in VEGF-treated group and VEGF+L-NAME-treated group com-pared with the control(P<0.05 and P<0.01).These results indicate that VEGF could promote the proliferation and secretion of PGI2 and inhibit the secretion of ET-1 and vWF in vascular endothelial cells and that L-NAME could inhibit the effect of VEGF partially.Nitric oxide is an important mediator in the process of stimulating prolifera-tion and regulating secretion of vascular endothelial cells by VEGF.展开更多
To evaluate the mechanism of vascular endothelial growth factor(VEGF)on the prevention of restenosis after angioplasty,the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into va...To evaluate the mechanism of vascular endothelial growth factor(VEGF)on the prevention of restenosis after angioplasty,the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into vascular smooth muscle cells(VSMC)in vitro.The conditioned medium containing VEGF was collected 72 h after the infection.Then,the VSMC and human umbilical vein endothelial cells(HUVEC)were divided into control group,H2O2-treated group and H2O2+VEGF-treated group to observe the proliferation and apoptosis by water soluble tetrazolium(WST-1)method,in situ nick end labeling(TUNEL)andflow cytometry(FCM).Compared with the control and H2O2+VEGF-treated groups,the absorbance(A)value of HUVEC was decreased,and apoptosis of HUVEC was significantly increased in H2O2-treated group.The changes of A value and apoptosis of VSMC were contrary to those of HUVEC.H2O2 could stimulate the proliferation of VSMC and induce the apoptosis of HUVEC,inhibit the proliferation of HUVEC and the apoptosis of VSMC and induce restenosis.VEGF could inhibit the effect of H2O2 on HUVEC and VSMC and prevent restenosis.These results offered further theoretical evidence for VEGF on the prevention of restenosis after angioplasty.展开更多
文摘The use of low-coherence light is expected to be one of the effective ways to suppress or even eliminate the laser–plasma instabilities that arise in attempts to achieve inertial confinement fusion.In this paper,a review of low-coherence high-power laser drivers and related key techniques is first presented.Work at typical low-coherence laser facilities,including Gekko XII,PHEBUS,Pharos III,and Kanal-2 is described.The many key techniques that are used in the research and development of low-coherence laser drivers are described and analyzed,including low-coherence source generation,amplification,harmonic conversion,and beam smoothing of low-coherence light.Then,recent progress achieved by our group in research on a broadband low-coherence laser driver is presented.During the development of our low-coherence high-power laser facility,we have proposed and implemented many key techniques for working with low-coherence light,including source generation,efficient amplification and propagation,harmonic conversion,beam smoothing,and precise beam control.Based on a series of technological breakthroughs,a kilojoule low-coherence laser driver named Kunwu with a coherence time of only 300 fs has been built,and the first round of physical experiments has been completed.This high-power laser facility provides not only a demonstration and verification platform for key techniques and system integration of a low-coherence laser driver,but also a new type of experimental platform for research into,for example,high-energy-density physics and,in particular,laser–plasma interactions.
基金Supported by Science Research Fund of Yunnan Provincial Education Department(2017ZZX090)School-level Key Project of Kunming University(XJZD1602)"Study on Key Technology of Ecological Planting Mode of Balcony Vegetables"of Provincial-level"Quality Project"of Kunming University-Innovation and Entrepreneurship Training Program for College Students
文摘To quantitatively analyze main figure,field,agency and level of sponge city research in China,and clear research focus and hot spot in each year,by using the Full Text Database of Chinese Sci-tech Periodicals and other retrieval tools,the statistics and analysis of 3152 research literatures on sponge city published in domestic academic journals of 2004-2016 are conducted based on bibliometrics. It is found that since the concept of " sponge city" was firstly proposed in 2012,development research of sponge city involves 40 subject fields and is mainly published in 32 kinds of journals,which is dominated by natural science research( 1427 literatures). Researchers are mainly from each college and university,some design institutes and Chinese Academy of Sciences. The research could play certain guidance significance for further research and construction of ecological city construction in China.
基金Supported by National Natural Science Foundation of China(31301665)
文摘Impatiens necrotic spot virus( INSV) is an important plant virus identified in recent years. Western flower thrip( Frankliniella occidentalis) has been confirmed to be the most efficient vector for INSV. In the present study,Illumina's second-generation sequencing technique was used to determine the variation in gene expression at the transcriptome level in western flower thrip before and after infection with INSV. By aligning 168 188 unigenes of western flower thrip to the NCBI protein database,47 558 unigenes were annotated,accounting for 28. 27% of the total. Species classification revealed that 58. 7% of the annotated unigenes of western flower thrip did not match any other species,and 15. 4% of had a match in Zootermopsis navadensis,12. 4% in Mus musculus,7. 3% in Mustela putorius,3. 4% in Theileria parva,and 2. 7% in Tribolium castaneum. By analyzing DEGs between INSV-free and INSV-carrying western flower thrips,we found that 11 genes were upregulated,and 9 genes were downregulated in western flower thrip after INSV infection. In addition,cell cycle and ubiquitin-proteasome pathway in western flower thrip were significantly infected by INSV infection.
基金This research was supported by the Science Foundation No. 2011FZ 178, 2011FZ 180, 2014HD004, 14C26215303260 and National Natural Science Foundation of China （NSFC） No. 31260073, 31340019, 31160412, 41361056.
文摘Wind turbines are usually designed and operated with fixed startup speed. It could perform startup and shutdown operations repeatedly when the wind fluctuates around the startup speed. The excessive stress induced by frequent startup and shutdown could enhance the likelihood of component failure and hence negatively impact the availability of a wind turbine. Startup speed with dead band is proposed in this article to prevent the wind turbine from frequent startup. 22 years wind data from the Cheung Chau wind station in Hong Kong are analyzed to evaluate the reduction in the number of startup and potential loss of wind power production using the proposed approach. Numerical simulation suggests that the number of startup could be reduced by half with trivial reduction in potential wind power generation in most of investigated sites once an appropriate dead band is adopted.
基金Supported by Natural Science Foundation of China(31460491)Applied Basic Research Project for Youth Scientists of Science and Technology Department of Yunnan Province(2013FD040)+2 种基金Scientific Research Project of Education Department of Yunnan Province(2014Y390)Talent Introduction Project of Kunming University(YJL14005)Fund for Construction of Characteristic Key Discipline(Ecology)in Colleges and Universities in Yunnan Province
文摘In order to investigate antifungnl effect of medicinal plant extracts on Botrytis cinerea, the antifungal activity of ethanol extracts of 8 medicinal plants in- eluding Ginkgo biloba against Botrytis cinerea was determined by growth rate method. The results showed that Ginkgo biloba and Ginnamomum cassia extracts had significant inhibition effects on B. cinema mycelium growth （P 〈 0.05）, with inhibition rates over 70% and ECho values of 0.027 mg/ml and 0.039 mg/ml, re- spectively, significantly different from the antifungal effects of other kinds of medicinal plants ; and P. ternata and A. tsaoko extracts showed the second highest in- hibition effects, with ECho values of 0. 011 9 and 0. 039 3 mg/ml, respectively. Further experimental application could be carried out.
文摘The'Internet+QQ farm'creative agriculture presented in this paper is the innovation based on the currently booming reality QQ farm,aimed at reducing cost,improving efficiency and optimizing human and material resource elements.It can make Internet provide technical support for intelligence-based life,leisure,entertainment,with good prospects for development.
文摘Sustainable building in China has gained attention both domestically and abroad. Despite the fast increase in sustainable assessment tools developed locally or adopted from overseas, there are still criticisms about the current situation of weak implementation and lack of comprehensive consideration. The lack of consideration of economic and social aspects or building performance on whole building life cycle all lead to departure from the true meaning of sustainable development. And lack of participation on the part of stakeholders makes it too theoretical to be carried out. This research aims to develop a model to address this problem. This research started with review of current sustainable assessment tools applied in China. As the assessment indicators have clear regional disparities, and almost no current tool considers all three pillars of environmental, economic and social in building life cycle. An industry survey was therefore designed for generation of indicators at different building stages, and personal interviews relevant to different occupation in building industry were conducted to complement the questionnaire survey. After that, the model Building Sustainable Score (BSS) was developed based on the stakeholders’ participation. Finally, the model is verified by a case study.
文摘Through the personnel training program,revising the syllabus,optimizing teaching contents,reforming teaching methods,strengthening practical teaching links and reforming examination methods,this paper explored the teaching reform and practice of Plant Growth Environment Course,in order to improve the teaching effect,stimulate learning interests of students,and cultivate application type talents meeting social demands.
基金Supported by the Science and Technology Planning Project of Yunan Tobacco Co.,Ltd.(2015YN36)
文摘Pesticide packaging wastes pose a serious hazard to the agricultural production environment and the quality of agricultural products. The investigation for 2 consecutive years in Jietou Region of Tengchong County, a major tobacco planting area in Yunan, showed that： ① More than 80% of the pesticides purchased by farmers were used for tobacco cultivation, which was 4 times that of conventional crops planting, and most of the packaging materials were plastic bags; ② The main way for farmers to dispose plastic bags was to burn or discard them in the field garbage collection baskets; ③ In combination with field surveys, an average of 4.54 pieces of pesticide packaging wastes were scattered around every 667 m^2 of tobacco field. In addition, the number reduced significantly after carrying out pesticide packaging wastes recycling and control project at the local smoke station. At the same time, the average number of pesticide packaging wastes in the field collection baskets increased. Therefore, it can effectively control pesticide packaging wastes by changing the type of pesticide packaging, encouraging the establishment of recycling system and strengthening the cognitive training of farmers.
基金Guangxi Traditional Chinese Medical University Scientific Research Project(XP021059)National Parasitic Resources Center(NPRC-2019-194-30)Key Technology Project of Inner Mongolia Science and Technology Department(2021GG0171)。
文摘Background:Food-borne parasitic diseases decrease food safety and threaten public health.The snail species is an intermediate host for numerous human parasitic trematodes.Orientogalba ollula has been reported as intermediate hosts of many zoonotic trematodes.Here,we investigated the prevalence of zoonotic trematodes withinO.ollula in Guangxi,China,and assessed their zoonotic potential.Methods:Snails were collected from 54 sites in 9 cities throughout Guangxi.The snail and trematode larvae species were determined by combining morphological characteristics and molecular markers.The trematodes prevalence and constituent ratio were calculated and compared among different habitat environments.Phylogenetic trees of the trematode species were constructed using the neighbor-joining method with nuclear internal transcribed spacer 2(ITS2)sequences.The developmental cycles of the isolated trematodes were examined by experimental infection in ducks.The developmental characteristics ofEchinostoma revolutum was recorded by dissecting infected ducklings from 1-day post infection(dpi)to 10 dpi.Results:The overall prevalence of trematode larvae was 22.1%(1818/8238)inO.ollula from 11 sample sites.Morphological together with molecular identification,showed thatE.revolutum,Australapatemon sp.,Hypoderaeum conoideum,Pharyngostomum cordatum,andEchinostoma sp.parasitizedO.ollula,with the highest infection rate ofE.revolutum(13.0%).However,noFasciola larvae were detected.The trematodes prevalence and constituent ratio varied in two sub-biotypes(P<0.01).A neighbor-joining tree analysis of ITS2 sequences resulted in distinct monophyletic clades supported by sequences from isolated larvae with high bootstrap values.Ducklings exposed toO.ollula infected withEchinostoma sp.,E.revolutum,andH.conoideum larvae were successfully infected.The animal model forEchinostoma revolutum was successfully established.E.revolutum matured from larvae to adult at 10 dpi in the intestine of the duck,and the developmental characteristics ofE.revolutum were characterized by the maturation of the reproductive and digestive organs at 6-8 dpi.Conclusions:This study revealed a high prevalence of zoonotic trematodes inO.ollula from Guangxi,China.Existing trematodes infection in animals and human clinical cases,coupled with the wide geographical distribution ofO.ollula,necessitate further evaluations of the potential risk of spillover of zoonotic infection from animal to human and vice versa.
基金supported by a grant from the Natural Science Foundation of Hubei Province(No.2003ABA135).
文摘To evaluate the role of nitric oxide in the biological effects of vascular endothelial growth factor(VEGF)and the possible mechanism of VEGF,the in vitro cultured vascular endothelial cells of rabbit aorta were divided into control group,VEGF-treated group and VEGF+N-nitro-L-arginine methyl ester(L-NAME)-treated group.The absorbance(A)value of vascular endothelial cells and the levels of prostaglandin(PGI2),endothelin-1(ET-1)and von Willebrand factor(vWF)in the supernatant were observed by water-soluble tetrazo-lium salt assay,radioimmunoassay and enzyme-linked immunosorbent assay.The A values and PGI2 in VEGF-treated group and VEGF+L-NAME-treated group were higher than those in control group(P<0.05 and P<0.01).The ET-1 and vWF were significantly decreased in VEGF-treated group and VEGF+L-NAME-treated group com-pared with the control(P<0.05 and P<0.01).These results indicate that VEGF could promote the proliferation and secretion of PGI2 and inhibit the secretion of ET-1 and vWF in vascular endothelial cells and that L-NAME could inhibit the effect of VEGF partially.Nitric oxide is an important mediator in the process of stimulating prolifera-tion and regulating secretion of vascular endothelial cells by VEGF.
基金supported by the Natural Science Foundation of Hubei Province of China(No.2003ABA135).
文摘To evaluate the mechanism of vascular endothelial growth factor(VEGF)on the prevention of restenosis after angioplasty,the recombinant adenovirus vector containing hVEGF165 cDNA was constructed and transfected into vascular smooth muscle cells(VSMC)in vitro.The conditioned medium containing VEGF was collected 72 h after the infection.Then,the VSMC and human umbilical vein endothelial cells(HUVEC)were divided into control group,H2O2-treated group and H2O2+VEGF-treated group to observe the proliferation and apoptosis by water soluble tetrazolium(WST-1)method,in situ nick end labeling(TUNEL)andflow cytometry(FCM).Compared with the control and H2O2+VEGF-treated groups,the absorbance(A)value of HUVEC was decreased,and apoptosis of HUVEC was significantly increased in H2O2-treated group.The changes of A value and apoptosis of VSMC were contrary to those of HUVEC.H2O2 could stimulate the proliferation of VSMC and induce the apoptosis of HUVEC,inhibit the proliferation of HUVEC and the apoptosis of VSMC and induce restenosis.VEGF could inhibit the effect of H2O2 on HUVEC and VSMC and prevent restenosis.These results offered further theoretical evidence for VEGF on the prevention of restenosis after angioplasty.
