β-Conglycinin is one of the major allergens existed in soybean.N-Glycans attached to theβ-conglycinin influenced the immunoreactivity and antigen presenting efficiency ofβ-conglycinin.In this study,we described a n...β-Conglycinin is one of the major allergens existed in soybean.N-Glycans attached to theβ-conglycinin influenced the immunoreactivity and antigen presenting efficiency ofβ-conglycinin.In this study,we described a new method used to release and collect the N-glycans fromβ-conglycinin,and the N-glycans existed in linear epitopes ofβ-conglycinin were identified.Glycopeptides hydrolyzed fromβ-conglycinin were purified by cotton hydrophilic chromatography.Trifluoromethylsulfonic acid was then used to release glycans from glycopeptides,and new glycopeptides containing one single N-acety1-D-glucosamine(G1 cNAc)moiety were then utilized for mass spectrometry.Five glycosylation sites(Asn-199,Asn-455,Asn-215,Asn-489 and Asn-326)and 22 kinds of glycopeptides were identified.It is noteworthy that the peptide VVN^(#)ATSNL(where^(#)represents for the glycosylation site)was analyzed to be both glycopeptide and linear epitope.Our results provided a new method for the N-glycoform analysis of food allergens,and laid a foundation for understanding the relationship between glyco sylation and food allergy.展开更多
In order to more clearly understand the relevance of the forestry land distribution and the terrain factors,basic information was given in this paper on the reasonable utilization of forest resources in mountainous ar...In order to more clearly understand the relevance of the forestry land distribution and the terrain factors,basic information was given in this paper on the reasonable utilization of forest resources in mountainous areas and the ecological environment protection.Based on the registration overlay analysis of high resolution aerial,satellite photograph and 1∶10 000 topographic map by making use of geostatistics of ArcGIS9.3,different photo color referred to different types of vegetation landscape,acquired factor data in small classes of woodland with visual interpretation in small classes of woodland and field research,and the correlation between forestry land and topography factors were discussed.It was shown that:in areas of low elevation and low slope where human beings were highly involved,all sorts of forestry land were distributed,which meant evolution and slope had little effect on forestry land,and that in those areas where human activities were lessened little by little with high elevation and high slope,kinds of forestry land decreased accordingly.Furthermore,the irrigated paddy fields were the main types of forestry land on the elevation of 1 200 m and slope above 35°.Differences among kinds of forestry land were comparatively indistinct from the perspective of slope.展开更多
基金funded by National Natural Science Foundation of China(31870798,31972024)Shaanxi Province Innovation Capability Support Plan-Science and Technology Innovation Team(2020TD-044)+2 种基金Key Laboratory of Glycobiology and Glycoengineering of Xi’an(2019219514SYS010CG032)Natural Science Project of Shaanxi Provincial Department of Education(21JK0929)Science and Technology Resources Sharing Platform Project of Science and Technology Department in Shaanxi Province(2022PT-46)。
文摘β-Conglycinin is one of the major allergens existed in soybean.N-Glycans attached to theβ-conglycinin influenced the immunoreactivity and antigen presenting efficiency ofβ-conglycinin.In this study,we described a new method used to release and collect the N-glycans fromβ-conglycinin,and the N-glycans existed in linear epitopes ofβ-conglycinin were identified.Glycopeptides hydrolyzed fromβ-conglycinin were purified by cotton hydrophilic chromatography.Trifluoromethylsulfonic acid was then used to release glycans from glycopeptides,and new glycopeptides containing one single N-acety1-D-glucosamine(G1 cNAc)moiety were then utilized for mass spectrometry.Five glycosylation sites(Asn-199,Asn-455,Asn-215,Asn-489 and Asn-326)and 22 kinds of glycopeptides were identified.It is noteworthy that the peptide VVN^(#)ATSNL(where^(#)represents for the glycosylation site)was analyzed to be both glycopeptide and linear epitope.Our results provided a new method for the N-glycoform analysis of food allergens,and laid a foundation for understanding the relationship between glyco sylation and food allergy.
基金Supported by National Science and Technology Project(2011BAC09B01 and 2006BAC01A16)Key Natural Science Foundation Project of Chongqing Municipality(CSTC2009BA0002)948 Project of State Bureau of Forestry(2009-4-20)
文摘In order to more clearly understand the relevance of the forestry land distribution and the terrain factors,basic information was given in this paper on the reasonable utilization of forest resources in mountainous areas and the ecological environment protection.Based on the registration overlay analysis of high resolution aerial,satellite photograph and 1∶10 000 topographic map by making use of geostatistics of ArcGIS9.3,different photo color referred to different types of vegetation landscape,acquired factor data in small classes of woodland with visual interpretation in small classes of woodland and field research,and the correlation between forestry land and topography factors were discussed.It was shown that:in areas of low elevation and low slope where human beings were highly involved,all sorts of forestry land were distributed,which meant evolution and slope had little effect on forestry land,and that in those areas where human activities were lessened little by little with high elevation and high slope,kinds of forestry land decreased accordingly.Furthermore,the irrigated paddy fields were the main types of forestry land on the elevation of 1 200 m and slope above 35°.Differences among kinds of forestry land were comparatively indistinct from the perspective of slope.
