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The dissolution of total suspended solids and treatment strategy of tailwater in a Litopenaeus vannamei recirculating aquaculture system
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作者 Yuhuan sun Jian LU +3 位作者 Tianlong QIU Li ZHOU jianming sun Yishuai DU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第3期1197-1205,共9页
In recirculating aquaculture systems(RASs),the effective treatment of aquaculture tailwater is essential to maintain the health of the RAS.This study investigated the optimal time and method for tailwater treatment du... In recirculating aquaculture systems(RASs),the effective treatment of aquaculture tailwater is essential to maintain the health of the RAS.This study investigated the optimal time and method for tailwater treatment during three periods of the aquaculture of the Litopenaeus vannamei:nursery(0–26 d),middle(27–57 d),and later(57–104 d).The variation of several water parameters during the dissolution of total suspended solid(TSS)in tailwater,applied with the effects of ozone on the microorganism and water quality parameters were investigated.Results showed that the TSS concentrations in tailwater decreased with time,although not significantly(P>0.05),whereas total ammonia nitrogen(TAN),nitrite(NO-2-N),and nitrate(NO_(3)^(-)-N)increased significantly(P<0.05).Therefore,TSS should be removed from the tailwater as early as possible,being most optimal within 4 h.Ozone removed 38.24%–48.95%of TSS,17.78%–90.14%of TAN,and 87.50%–98.90%of NO-2-N after 4 h of treatment.However,it resulted in the significant accumulation of NO_(3)^(-)-N.Moreover,the total number of Vibrio and bacterial counts in aquaculture tailwater was reduced completely by ozone within 4 h.Thus,these results provided technical details and data support for the effective treatment of tailwater from shrimp RAS. 展开更多
关键词 DISSOLUTION Litopenaeus vannamei OZONATION recirculating aquaculture system(RAS) tailwater treatment total suspended solid
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Influencing factors of hospitalization costs for glaucoma patients under clinical pathway management
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作者 Bomin Lin Yangchu Li +5 位作者 Wei Zhang Xiqing Liao Huiming Xiao jianming sun Yanwei Lin Aihua Lin 《Annals of Eye Science》 2019年第1期152-160,共9页
Background:To investigate and analyze the hospitalization costs of inpatients with primary acute angle closure glaucoma(PACG),and to explores the influencing factors of hospitalization cost and to provide reference fo... Background:To investigate and analyze the hospitalization costs of inpatients with primary acute angle closure glaucoma(PACG),and to explores the influencing factors of hospitalization cost and to provide reference for specialized hospitals to carry out clinical pathways.Methods:The first page diagnostic data of PACG patients’medical records were collected,and an Excel database was established according to the International Classification of Diseases(ICD-10)code.Statistical analysis of hospitalization data was performed using SPSS 17.0 software.Results:Hospitalization days and clinical pathway which affect the change of the hospitalization cost(P<0.001).Conclusions:Hospitalization day is an important factor affecting the hospitalization cost,reducing unnecessary hospitalization time can control the increase of hospitalization cost. 展开更多
关键词 Clinical pathway GLAUCOMA hospitalization cost statistical analysis
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人大隐静脉干细胞原代培养方法的改良 被引量:1
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作者 凌思英 张柏杨 +5 位作者 滕勇 魏巍 喻武 孙建明 唐博 陈以宽 《生物工程学报》 CAS CSCD 北大核心 2018年第6期973-982,共10页
旨在探讨如何高效获取优质的人大隐静脉(Great saphenous vein)原代干细胞。大隐静脉剪碎后分别采用组织块贴壁法和Ⅱ型胶原酶消化法获取血管壁细胞。倒置显微镜下观察不同时间段两组血管壁细胞形态学变化,台盼蓝染色测定血管壁细胞存活... 旨在探讨如何高效获取优质的人大隐静脉(Great saphenous vein)原代干细胞。大隐静脉剪碎后分别采用组织块贴壁法和Ⅱ型胶原酶消化法获取血管壁细胞。倒置显微镜下观察不同时间段两组血管壁细胞形态学变化,台盼蓝染色测定血管壁细胞存活率,流式分选CD34和CD117双阳性干细胞,免疫荧光进一步证实。细胞培养至第三代(P3)时组织块贴壁法提取的细胞出现纤维化老化,而酶消化法提取的细胞仍有集落样生长,细胞存活率分别为(91.7±1.2)%和(97.2±0.7)%(P=0.005)。流式分选结果显示:组织块法和酶消化法获得CD34和CD117双阳性细胞所占比例分别为(0.16±0.05)%和(0.44±0.07)%,差异有统计学意义(P=0.005)。免疫荧光染色显示,分选出的干细胞培养1周后组织块法获得双阳性干细胞的阳性率(89.41±2.06)%,酶消化法为(94.03±1.83)%,P<0.05。流式细胞仪检测分选出的干细胞中CD31、VEGF2和SMA阳性率分别为(0.12±0.01)%、(0.19±0.02)%和(0.45±0.01)%,与阴性对照组差异无统计学意义(P>0.05),排除成熟内皮细胞和平滑肌细胞存在的可能性。分选出的干细胞进行管腔形成实验进一步证实其具有向内皮分化的潜能。上述结果显示,采用酶消化法可以获得形态更好、数量更多、存活率相对更高的干细胞,可广泛用于临床和基础研究。 展开更多
关键词 大隐静脉 血管干细胞 原代培养 组织块贴壁法 酶消化法
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生物补片应用于腹腔镜全腹膜外成人腹股沟疝修补的疗效分析 被引量:1
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作者 吴明东 刘俊杰 +6 位作者 喻武 张明逸 周好男 冉坤 孙建明 唐博 陈以宽 《中华疝和腹壁外科杂志(电子版)》 2022年第5期525-530,共6页
目的探讨生物补片应用于成人腹股沟疝腹腔镜全腹膜外修补术(TEP)的早期临床安全性和有效性。方法回顾性分析2020年5月至2022年1月在重庆医科大学附属第二医院接受生物补片TEP修补术的75例腹股沟疝患者的临床和随访资料,观察术后复发、... 目的探讨生物补片应用于成人腹股沟疝腹腔镜全腹膜外修补术(TEP)的早期临床安全性和有效性。方法回顾性分析2020年5月至2022年1月在重庆医科大学附属第二医院接受生物补片TEP修补术的75例腹股沟疝患者的临床和随访资料,观察术后复发、早期反应和并发症情况。结果纳入腹股沟疝患者75例(102侧)。均顺利完成手术,无中转开腹情况。单侧腹股沟疝平均手术时间为(59.0±9.2)min,双侧平均手术时间为(86.4±15.0)min。术后中等度热6例(8.0%),血清肿11例(13侧,12.7%),中度及以上急性疼痛16例(18侧,17.6%)。平均随访时间(9.7±5.7)个月,疝复发3例(2.9%),慢性疼痛1例(1.3%)。结论应用生物补片行TEP修补术安全有效。生物补片的术后早期反应主要为发热、血清肿和疼痛,妥善缝合固定补片可减少术后复发。 展开更多
关键词 腹股沟 疝修补术 腹腔镜 生物补片 疗效
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