[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the qu...[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the quercetin was quantitatively analyzed by HPLC.[Results]There was a good linear relationship between the peak area(A)and the content(M)of quercetin in the range of 0.048-0.168μg,A=2615.5M+1.5286,r=0.9999.The method was applied to the determination of ethanol extract of H.littoralis leaves,the average recovery rate was 98.77%and RSD was 1.57%.[Conclusions]The method is simple,convenient,reproducible and accurate,and provides an idea for the determination of quercetin in leaves of H.littoralis.展开更多
基金Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GXZYKF2019-7).
文摘[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the quercetin was quantitatively analyzed by HPLC.[Results]There was a good linear relationship between the peak area(A)and the content(M)of quercetin in the range of 0.048-0.168μg,A=2615.5M+1.5286,r=0.9999.The method was applied to the determination of ethanol extract of H.littoralis leaves,the average recovery rate was 98.77%and RSD was 1.57%.[Conclusions]The method is simple,convenient,reproducible and accurate,and provides an idea for the determination of quercetin in leaves of H.littoralis.
