To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags （ESTs） from the muscle cDNA database of the mandarin fish （Siniperca chuatsi）. C...To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags （ESTs） from the muscle cDNA database of the mandarin fish （Siniperca chuatsi）. Clustering analysis yielded 1,625 unique sequences including 443 contigs （from 3,881 EST sequences） and 1,182 sin- gletons. BLASTX searches showed that 959 unique sequences shared homology to proteins in the NCBI non-redundant database. A total of 740 unique sequences were functionally annotated using Gene Ontology. The 1,625 unique sequences were assigned to Kyoto Encyclopedia of Genes and Genomes reference pathways, and the results indicated that transcripts participating in nucleotide metabolism and amino acid metabolism are relatively abundant in S. chuatsi. Meanwhile, we identified 15 genes to be abundantly expressed in muscle of the mandarin fish. These genes are involved in muscle structural formation and regulation of muscle differentiation and development. The most remarkable gene in S. chuatsi is nuclease diphosphate kinase B, which is represented by 449 EST sequences accounting for 8.86% of the total EST sequences. Our work provides a transcript profile expressed in the white muscle of the mandarin fish, laying down a foundation in better understanding of fish genomics.展开更多
Fishery is an important industry in China as well as in the rest of the world,and it provides a human food resource containing high-quality protein.Best practice in aquaculture requires a full understanding of the gen...Fishery is an important industry in China as well as in the rest of the world,and it provides a human food resource containing high-quality protein.Best practice in aquaculture requires a full understanding of the genomic controls and transcriptional profiles of cultured fish species.Improvements in aquaculture can be made by regulation of the expression of functional genes.Microarray technology is a powerful tool for rapid screening of genes or transcriptional profiles in a particular fish or for a particular economic character;for example,genes that are related to growth and disease control in the fish.This review provides a brief introduction to microarray technology and its methods and applications,together with a discussion of the achievements in fish biology that have resulted from this technology.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 30972263 and 30771644)the Natural Science Foundation of Hunan Province, China (Grant No. 09JJ6037 and 08jj3064)
文摘To enrich the genomic information of the commercially important fish species, we obtained 5,063 high-quality expressed sequence tags （ESTs） from the muscle cDNA database of the mandarin fish （Siniperca chuatsi）. Clustering analysis yielded 1,625 unique sequences including 443 contigs （from 3,881 EST sequences） and 1,182 sin- gletons. BLASTX searches showed that 959 unique sequences shared homology to proteins in the NCBI non-redundant database. A total of 740 unique sequences were functionally annotated using Gene Ontology. The 1,625 unique sequences were assigned to Kyoto Encyclopedia of Genes and Genomes reference pathways, and the results indicated that transcripts participating in nucleotide metabolism and amino acid metabolism are relatively abundant in S. chuatsi. Meanwhile, we identified 15 genes to be abundantly expressed in muscle of the mandarin fish. These genes are involved in muscle structural formation and regulation of muscle differentiation and development. The most remarkable gene in S. chuatsi is nuclease diphosphate kinase B, which is represented by 449 EST sequences accounting for 8.86% of the total EST sequences. Our work provides a transcript profile expressed in the white muscle of the mandarin fish, laying down a foundation in better understanding of fish genomics.
基金supported by the Natural Science Foundation of China(Grant Nos.30640015 and 30771644).
文摘Fishery is an important industry in China as well as in the rest of the world,and it provides a human food resource containing high-quality protein.Best practice in aquaculture requires a full understanding of the genomic controls and transcriptional profiles of cultured fish species.Improvements in aquaculture can be made by regulation of the expression of functional genes.Microarray technology is a powerful tool for rapid screening of genes or transcriptional profiles in a particular fish or for a particular economic character;for example,genes that are related to growth and disease control in the fish.This review provides a brief introduction to microarray technology and its methods and applications,together with a discussion of the achievements in fish biology that have resulted from this technology.
