BACKGROUND: Scholars have supposed to establish animal models of optic neuropathy by pressing and partially amputating optic nerve, increasing intraocular pressure and injecting vasoconstrictor, etc., but the models ...BACKGROUND: Scholars have supposed to establish animal models of optic neuropathy by pressing and partially amputating optic nerve, increasing intraocular pressure and injecting vasoconstrictor, etc., but the models are greatly different from anterior ischemia optic neuropathy. Therefore, a more ideal method is needed to establish animal model of anterior ischemic optic neuropathy (AION). OBJECTIVE : To establish AION models in rats, observe the functional changes of fundus, fundus fluorescein angiography (FFA), optical coherence tomography (OCT), flash visual evoked potential (F-VEP), and histopathologically confirm its reliability. DESIGN: A randomized control tria SETTINGS : Department of Ophthalmology, Xi'an Fourth Hospital; Xi'an Institute of Ocular Fundus Diseases MATERIALS : The experiments were carried out in the research room of Xi'an Institute of Ocular Fundus Diseases from February 2005 to May 2006. Thirty healthy male SD rats of 4-5 weeks old, weighing 140-160 g, were provided by the animal experimental center of the Fourth Military Medical University of Chinese PLA [SCXK (Military)2002-005], and those without eye disease examined by slit lamp and direct ophthalmoscope after mydriasis were enrolled. The conditions for feeding mice without special pathogen were strictly followed. The rats were randomly divided into blank control group (n =5), laser group (n =5), hematoporphyrin derivative (HPD) group and AION group (n =15), each group was numbered randomly. For each rat, the right eye was taken as the experimental eye, and the left one as the control one. METHODS: In the AION group, the rats were injected with HPD (10 mg/kg) via caudal vein, and then the optic discs were exposed to krypton red (647 nm, 80 mV) for 120 s, and the rats were in avoidance of light for 2 weeks postoperatively. Rats in the laser group were only exposed to krypton red (647 rim, 80 mV) for 120 s, and in avoidance of light for 2 weeks postoperatively; Those in the HPD group were only injected with HPD (10 mg/kg) via caudal vein; Those in the blank control group were untouched. (1) Visual electrophysiological test: The F-VEP was used to evaluate the function of visual nerve. (2) FFA: After mydriasis and anesthesia as describe above, the fluorescein sodium parenteral solution (1 mL/kg) was injected v/a caudal vein and finished within about 3 s, the time of FFA was recorded from the beginning of injection, the video sight aimed at the optic disc and the surrounding area. (3) After mydriasis and anesthesia as describe above, the rats were examined with OCT. (4) Histological observation: After hematoxylin and eosin (HE) staining, the optic disc and surrounding blood vessels of retina were observed under light microscope at high power field. MAIN OUTCOME MEASURES: The results of fundus, FFA, visual electrophysiological test and OCT detection within 90 days after model establishment were observed. RESULTS: Of the 30 rats, 1 died after anesthesia in the laser group and 2 died in the AION group respectively, and finally 27 rats were involved in the analysis of results. (1) Changes in fundus: In the AION group, there was edema in upper optic disc and unclear boundary at 1 day after establishment, edema still could be observed at 6 days, and upper optic disc atrophied and appeared as pale at 90 days. (2) FFA results: In the AION group, early "low fluorescence", middle and late "high fluorescence" were observed in upper optic disc 1 day after model establishment, and there was "low fluorescence" at 6 days, and the low fluorescence could be observed all the time at 23 days. (3) Visual electrophysiological changes: In the AION group as compared with the control eyes, the experimental ones had prolonged F-VEP P100 latency [(71.65±8.81), (57.58±8.38) ms, t =3.148, P =0.012], and decreased wave amplitude [(4.77±1.90), (10.06±3.66) μV, t =4.082, P =0.003], and these changes lasted to 35 days after model establishment. (4) OCT results: In the AION group, the reflection surface of part nerve fiber layer was higher than the retina plane, the surface was rough and the thickness was increased at 6 days after model establishment. (5) Histopathological results: At 1 day after model establishment, part optic discs had highly edema, edema of nerve fibers, and loose tissue, also accompanied by the displacement of surrounding retina; At 23 days, the optic disc and surrounding nerve fiber layers became thinner, and the numbers of ganglion nuclei in the retina tissue sections were obviously decreased. These changes were not observed in the laser group, HPD group and blank control group. CONCLUSION : The changes of fundus, FFA, OCT, visual electrophysiology and histopathology confirmed that the krypton red laser irradiation (647 nm) at 2 hours after HPD was injected via caudal vein can establish more ideal animal models of AION.展开更多
We classified forest resources into four modes:high timber output and high ecological reserve(Mode T-E); high timber output and low ecological reserve(Mode T-e); low timber output and low ecological reserve(Mode...We classified forest resources into four modes:high timber output and high ecological reserve(Mode T-E); high timber output and low ecological reserve(Mode T-e); low timber output and low ecological reserve(Mode t-e); and low timber output and high ecological reserve(Mode t-E). Ecological reserve is stand volume per unit area of natural forests and total area of natural forests;timber output is defined as total area of timber forests and unit area of timber production. We used this classification system to examine forest development in China between1950 and 2013. Data were acquired mainly from forest inventory statistics published by China’s Forestry Administration between the 1970 s and 2013. I Information from the 1950 s was acquired from relevant literature. Our analysis suggests that China’s forest resources transitioned from Mode t-E to Mode T-e during the period between the early 1950 s and late 1970 s, resulting in the destruction of both ecological vigor and timber resources. During the following 20 years, strategies were implemented to improve the ecological reserve and increase timber supply,resulting in a decline in the rate of forest degradation. Over the past decade, China’s forest resources have reached Mode T-E as a result of improvements in both the ecological reserve and the timber supply. Currently, the total area of timber forests is relatively low, representing the limiting factor for improvement in overall forest functionality. Nevertheless, along with increased efforts to protect natural forests and develop fast-growing forest plantations, it is hopeful that China’s forest resources will achieve a sustainable state. The four-mode TOER(timber output, ecological reserve) method introduced in this paper is a simple but an effective approach for characterizing the overall quality and quantity of forest resources. The data used for this type of evaluation are typically easy to obtain and reliable. This methodology has potential to be applied to forests in various regions and countries.展开更多
Tuning surface electron transfer process by sulfur(S)-vacancies engineering is an efficient strategy to develop high-efficient catalysts for electroreduction N_(2) reaction(NRR). Herein, the distinct Sb_(2)S_(3) nanor...Tuning surface electron transfer process by sulfur(S)-vacancies engineering is an efficient strategy to develop high-efficient catalysts for electroreduction N_(2) reaction(NRR). Herein, the distinct Sb_(2)S_(3) nanorods with S-vacancies(Sv-Sb_(2)S_(3)) have been synthesized by a simple twostep method including hydrothermal and hydrogenation in H_(2)/Ar atmosphere, which shows improved performance for NRR with the NH_(3) yield rate of 10.85 μg h^(-1) mgcat^(-1) at-0.4 V vs. RHE, the faradaic efficiency(FE) of 3.75% at -0.3 V vs. RHE and excellent stability for 24 h, largely outperforming bulk Sb_(2)S_(3). X-ray photoelectron spectroscopy(XPS) and density function theory(DFT) calculations demonstrate that the abundant S-vacancies can create an electron-deficient environment and modulate the electron delocalization in Sv-Sb_(2)S_(3), which can not only facilitate the N_(2) molecule adsorption, but also activate the N≡N, resulting in the enhanced performance for NRR.展开更多
基金a grant from Scientific Research Fund of Shaanxi Department of Hygiene, No. 04AC1
文摘BACKGROUND: Scholars have supposed to establish animal models of optic neuropathy by pressing and partially amputating optic nerve, increasing intraocular pressure and injecting vasoconstrictor, etc., but the models are greatly different from anterior ischemia optic neuropathy. Therefore, a more ideal method is needed to establish animal model of anterior ischemic optic neuropathy (AION). OBJECTIVE : To establish AION models in rats, observe the functional changes of fundus, fundus fluorescein angiography (FFA), optical coherence tomography (OCT), flash visual evoked potential (F-VEP), and histopathologically confirm its reliability. DESIGN: A randomized control tria SETTINGS : Department of Ophthalmology, Xi'an Fourth Hospital; Xi'an Institute of Ocular Fundus Diseases MATERIALS : The experiments were carried out in the research room of Xi'an Institute of Ocular Fundus Diseases from February 2005 to May 2006. Thirty healthy male SD rats of 4-5 weeks old, weighing 140-160 g, were provided by the animal experimental center of the Fourth Military Medical University of Chinese PLA [SCXK (Military)2002-005], and those without eye disease examined by slit lamp and direct ophthalmoscope after mydriasis were enrolled. The conditions for feeding mice without special pathogen were strictly followed. The rats were randomly divided into blank control group (n =5), laser group (n =5), hematoporphyrin derivative (HPD) group and AION group (n =15), each group was numbered randomly. For each rat, the right eye was taken as the experimental eye, and the left one as the control one. METHODS: In the AION group, the rats were injected with HPD (10 mg/kg) via caudal vein, and then the optic discs were exposed to krypton red (647 nm, 80 mV) for 120 s, and the rats were in avoidance of light for 2 weeks postoperatively. Rats in the laser group were only exposed to krypton red (647 rim, 80 mV) for 120 s, and in avoidance of light for 2 weeks postoperatively; Those in the HPD group were only injected with HPD (10 mg/kg) via caudal vein; Those in the blank control group were untouched. (1) Visual electrophysiological test: The F-VEP was used to evaluate the function of visual nerve. (2) FFA: After mydriasis and anesthesia as describe above, the fluorescein sodium parenteral solution (1 mL/kg) was injected v/a caudal vein and finished within about 3 s, the time of FFA was recorded from the beginning of injection, the video sight aimed at the optic disc and the surrounding area. (3) After mydriasis and anesthesia as describe above, the rats were examined with OCT. (4) Histological observation: After hematoxylin and eosin (HE) staining, the optic disc and surrounding blood vessels of retina were observed under light microscope at high power field. MAIN OUTCOME MEASURES: The results of fundus, FFA, visual electrophysiological test and OCT detection within 90 days after model establishment were observed. RESULTS: Of the 30 rats, 1 died after anesthesia in the laser group and 2 died in the AION group respectively, and finally 27 rats were involved in the analysis of results. (1) Changes in fundus: In the AION group, there was edema in upper optic disc and unclear boundary at 1 day after establishment, edema still could be observed at 6 days, and upper optic disc atrophied and appeared as pale at 90 days. (2) FFA results: In the AION group, early "low fluorescence", middle and late "high fluorescence" were observed in upper optic disc 1 day after model establishment, and there was "low fluorescence" at 6 days, and the low fluorescence could be observed all the time at 23 days. (3) Visual electrophysiological changes: In the AION group as compared with the control eyes, the experimental ones had prolonged F-VEP P100 latency [(71.65±8.81), (57.58±8.38) ms, t =3.148, P =0.012], and decreased wave amplitude [(4.77±1.90), (10.06±3.66) μV, t =4.082, P =0.003], and these changes lasted to 35 days after model establishment. (4) OCT results: In the AION group, the reflection surface of part nerve fiber layer was higher than the retina plane, the surface was rough and the thickness was increased at 6 days after model establishment. (5) Histopathological results: At 1 day after model establishment, part optic discs had highly edema, edema of nerve fibers, and loose tissue, also accompanied by the displacement of surrounding retina; At 23 days, the optic disc and surrounding nerve fiber layers became thinner, and the numbers of ganglion nuclei in the retina tissue sections were obviously decreased. These changes were not observed in the laser group, HPD group and blank control group. CONCLUSION : The changes of fundus, FFA, OCT, visual electrophysiology and histopathology confirmed that the krypton red laser irradiation (647 nm) at 2 hours after HPD was injected via caudal vein can establish more ideal animal models of AION.
基金supported by China’s National Key Reaserch and Development Plan(2016YFC0502902)
文摘We classified forest resources into four modes:high timber output and high ecological reserve(Mode T-E); high timber output and low ecological reserve(Mode T-e); low timber output and low ecological reserve(Mode t-e); and low timber output and high ecological reserve(Mode t-E). Ecological reserve is stand volume per unit area of natural forests and total area of natural forests;timber output is defined as total area of timber forests and unit area of timber production. We used this classification system to examine forest development in China between1950 and 2013. Data were acquired mainly from forest inventory statistics published by China’s Forestry Administration between the 1970 s and 2013. I Information from the 1950 s was acquired from relevant literature. Our analysis suggests that China’s forest resources transitioned from Mode t-E to Mode T-e during the period between the early 1950 s and late 1970 s, resulting in the destruction of both ecological vigor and timber resources. During the following 20 years, strategies were implemented to improve the ecological reserve and increase timber supply,resulting in a decline in the rate of forest degradation. Over the past decade, China’s forest resources have reached Mode T-E as a result of improvements in both the ecological reserve and the timber supply. Currently, the total area of timber forests is relatively low, representing the limiting factor for improvement in overall forest functionality. Nevertheless, along with increased efforts to protect natural forests and develop fast-growing forest plantations, it is hopeful that China’s forest resources will achieve a sustainable state. The four-mode TOER(timber output, ecological reserve) method introduced in this paper is a simple but an effective approach for characterizing the overall quality and quantity of forest resources. The data used for this type of evaluation are typically easy to obtain and reliable. This methodology has potential to be applied to forests in various regions and countries.
基金supported by Natural Science Foundation of China (NSFC no. 21673105)the Science and Technology Program of Gansu Province of China (Grant No.1717JR5RA194)。
文摘Tuning surface electron transfer process by sulfur(S)-vacancies engineering is an efficient strategy to develop high-efficient catalysts for electroreduction N_(2) reaction(NRR). Herein, the distinct Sb_(2)S_(3) nanorods with S-vacancies(Sv-Sb_(2)S_(3)) have been synthesized by a simple twostep method including hydrothermal and hydrogenation in H_(2)/Ar atmosphere, which shows improved performance for NRR with the NH_(3) yield rate of 10.85 μg h^(-1) mgcat^(-1) at-0.4 V vs. RHE, the faradaic efficiency(FE) of 3.75% at -0.3 V vs. RHE and excellent stability for 24 h, largely outperforming bulk Sb_(2)S_(3). X-ray photoelectron spectroscopy(XPS) and density function theory(DFT) calculations demonstrate that the abundant S-vacancies can create an electron-deficient environment and modulate the electron delocalization in Sv-Sb_(2)S_(3), which can not only facilitate the N_(2) molecule adsorption, but also activate the N≡N, resulting in the enhanced performance for NRR.