Red,white,blue,green,and yellow lights were applied to investigate their effects on folate accumulation in wheat seedlings.The different lights,especially red light,significantly increased the total folate content.Tot...Red,white,blue,green,and yellow lights were applied to investigate their effects on folate accumulation in wheat seedlings.The different lights,especially red light,significantly increased the total folate content.Total folate showed maximum accumulation under 30μmol/(m2·s)of red light,with an increase of 24%compared with the control(darkness).5-Methyltetrahydrofolate(5-CH3-THF)was the dominant folate component,and was significantly increased by red light irradiation.In addition,under red light,the folate content of leaves was higher and more sensitive to light than that of endosperm or roots.Red light up-regulated the expression of guanosine triphosphate(GTP)cyclohydrolase 1(GCH1)and aminodeoxychorismate synthase(ADCS),enhanced the activity of GCH1 and ADCS,and increased the content of precursors of folate synthesis,including pterin and p-aminobenzoic acid(p ABA).Hence,the increased folate accumulation promoted by light could be attributed to the increased content of folate synthesis precursors,the activity of key enzymes,and related gene expression。展开更多
基金the National Natural Science Foundation of China(No.31871725)the Fundamental Research Funds for the Central Universities(No.KYYZ202004)+1 种基金the Zhenjiang Key R&D Plan(No.NY2020021)the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions,China。
文摘Red,white,blue,green,and yellow lights were applied to investigate their effects on folate accumulation in wheat seedlings.The different lights,especially red light,significantly increased the total folate content.Total folate showed maximum accumulation under 30μmol/(m2·s)of red light,with an increase of 24%compared with the control(darkness).5-Methyltetrahydrofolate(5-CH3-THF)was the dominant folate component,and was significantly increased by red light irradiation.In addition,under red light,the folate content of leaves was higher and more sensitive to light than that of endosperm or roots.Red light up-regulated the expression of guanosine triphosphate(GTP)cyclohydrolase 1(GCH1)and aminodeoxychorismate synthase(ADCS),enhanced the activity of GCH1 and ADCS,and increased the content of precursors of folate synthesis,including pterin and p-aminobenzoic acid(p ABA).Hence,the increased folate accumulation promoted by light could be attributed to the increased content of folate synthesis precursors,the activity of key enzymes,and related gene expression。
